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Gene information
General information
TC005457 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12810625..12815321
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005457
    iBeetle screen iB_09400

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005457 RA
      MNSLEPVPV QSVSIGPDS KLWKMKQLV SAIQLYFQT 
      KREDTATVI NMHLQSLSV TIDLSVFDP HSTIAPQFF 
      VSLYELMNK MEQRSSFAW NCVDVLANI CRNPAARSA 
      LIHTYQFIP SLSRLLSDQ LSNEKKIRL LRLMQDLTC 
      GIKISWQIP HLPQLMVTL SKWIETSNE EIITLSMGI 
      LVNLCYKNL SAIYTLFGN VDVKKILQI CSLMRGPTI 
      EIHTCKMLI FLDYLNGDI PENILLKIT NVTFESVVE 
      AFRIRDSIL LRQIVEFFL DVGKQNTLS VLVQSQDYS 
      KQLTAVLDI IQNNHGMET GDSTFECEP ECFALVFEF 
      LGFLTEIKV PAVTSKYAD LIQIALKWI HTDLVSSQA 
      LALLKSIAV NVDENKMEI LEPLVQGLP IFLNTLDSS 
      RNEDGPAYV EQNLKIGGL LQLLRIMTQ VATIRPKIL 
      EDLKVATVC KVLGCLQDA ENVSNSKTN DLDMANIDT 
      VNLYVHGIA LVNELAKHD ITWMNLQTN LMQHKQTHM 
      LLAQCLYQS SKEEKALVF EMSTIRCFP RKSVADAMV 
      ALQAATAPD LKSSSHSIQ SQDLNFPVL SVTQINKVD 
      SLLSKLREN FTQKQFGSV LTSDVIELY EYKLASMGN 
      AERAAMASV EAASERCTH LQHRNAQLS AELNRSHQL 
      LYHVQQCHE QVSKLTESL EEKVETLKK QSEVDRGKL 
      KVCKSQLGV KEDELNECK AELESYKKK YEKSVAVRR 
      EVEEQNAQL KQYIAKLEE NSTKLQKLV QKKEEALKN 
      TNLKVSELE QGLTQKENE LHQAKAELQ ETTKELNTR 
      KQILETITK FAMNSTRLE Q
    CDS sequence
    • >TC005457 RA
      atgaattcc ctggaacca gtcccagtg cagtccgtt 
      tccatcggc cccgactcc aaactgtgg aaaatgaag 
      cagctcgtg agcgcaatc cagctatat ttccagaca 
      aaacgcgaa gacaccgcc acagtaatc aacatgcac 
      ctccagagc ctgagtgtg accatagac ttgagcgtc 
      ttcgacccg cactcgaca atcgccccc cagtttttc 
      gtcagtctg tacgagctt atgaacaaa atggagcag 
      cggtcgtcc tttgcctgg aactgcgtc gacgttttg 
      gccaacatt tgccgcaac cccgcggcc cgcagcgcc 
      ctaatccac acctatcag ttcattccg agtctgtcg 
      cggctgttg agcgaccag ttatcaaac gagaaaaaa 
      atccggctg ttgagactc atgcaggac ctcacttgc 
      ggcatcaaa atctcgtgg caaatcccg cacttgccc 
      cagctcatg gtcacgctg agcaagtgg atcgagacc 
      tccaacgag gagataatc acgctctcg atggggatt 
      ttggtcaat ttgtgttac aaaaaccta tcggcgatt 
      tacacgctt ttcgggaac gtggacgta aagaaaata 
      ctccagatt tgttcgctg atgaggggc ccaactatt 
      gaaatacac acgtgcaaa atgttgatt tttttggac 
      tacttgaac ggcgatatt cccgaaaat attctcctg 
      aaaatcaca aatgtgact tttgagtcg gttgttgaa 
      gcttttcgg atacgagac tcgattttg ttgcgacaa 
      attgtcgaa tttttcctt gatgtgggc aagcaaaac 
      acgcttagt gttttagta caaagtcag gactatagt 
      aagcagctc acagccgtc ttggatatt atacaaaat 
      aatcacgga atggagacc ggcgacagc actttcgag 
      tgcgaaccc gaatgtttc gctttagtt ttcgaattt 
      ttgggtttt ttgacggaa atcaaagtc ccggcagtg 
      acttcaaag tacgctgat ttgatccaa atcgcctta 
      aagtggatt cacaccgac ttggtgtca tcccaagct 
      ttggctctt ttaaaatca attgccgta aacgtggac 
      gaaaacaaa atggagatt ttggagcct ctcgttcag 
      ggacttccc atttttttg aacactttg gacagcagt 
      cgtaacgaa gatggccct gcttacgtc gaacagaat 
      ttaaaaatc ggcggcttg ttacaactg ttacgaatt 
      atgacacaa gttgcgaca attcggcct aaaattttg 
      gaagatttg aaagttgca acggtttgt aaagttttg 
      gggtgtctc caagacgct gaaaacgtc tcaaatagt 
      aaaacaaac gacctggac atggccaac attgacacg 
      gttaattta tacgtccac ggaatagcc ctagttaac 
      gaattggcc aagcatgac atcacgtgg atgaacctt 
      cagaccaat ctcatgcag cacaagcaa acccacatg 
      ttgctagcg cagtgcctg taccaaagt tcgaaagag 
      gaaaaagcg ctagttttt gaaatgtcg acaattcgg 
      tgtttcccc cgaaagagc gtcgctgac gcgatggtg 
      gcgctccaa gcggcgaca gcgcccgac cttaaaagc 
      tcctcccac agcatccaa tcacaagac ttgaatttc 
      ccggttctt tcggttaca caaatcaac aaagttgat 
      tcgctcctc tccaaactg cgcgaaaac ttcacccag 
      aagcagttc ggttcggtt ctaacctct gacgtcatc 
      gagttgtac gagtacaag ttggcgtcg atgggcaat 
      gcagagcgc gctgcgatg gcaagtgtc gaggcggcg 
      tccgagcgc tgcacgcac ttgcagcac aggaacgcc 
      caattgagt gccgaatta aatcgctcg catcaattg 
      ttataccac gtgcaacag tgccacgag caggtctcg 
      aaattaacc gagtcgttg gaagaaaaa gtcgaaact 
      ttgaaaaag cagtcagag gttgaccga ggcaagttg 
      aaagtgtgt aaaagtcag ctgggggtg aaggaggac 
      gagctaaac gagtgcaaa gccgagctg gagagttac 
      aagaagaaa tatgagaag agtgtggcg gtcaggagg 
      gaggtggag gagcagaac gcccagttg aagcaatac 
      atcgccaaa ttggaggag aattcgacc aagttgcag 
      aaactggtg cagaagaag gaggaggcg ctgaaaaac 
      acgaatttg aaagtgagc gagttggag caaggtttg 
      acgcaaaaa gagaacgag ttgcaccag gcgaaggcc 
      gagctgcaa gaaaccacc aaagaatta aatacccgg 
      aaacaaatt ttagaaact atcacgaaa ttcgccatg 
      aactcgacg cgattggag caataa
    mRNA sequence
    • >TC005457 RA
      ggtttacaa tctcctgtc acctgtcat tttcaaatc 
      gctcgcttt attattatt ttcttttat tttgtttaa 
      tgttttacg atgaattcc ctggaacca gtcccagtg 
      cagtccgtt tccatcggc cccgactcc aaactgtgg 
      aaaatgaag cagctcgtg agcgcaatc cagctatat 
      ttccagaca aaacgcgaa gacaccgcc acagtaatc 
      aacatgcac ctccagagc ctgagtgtg accatagac 
      ttgagcgtc ttcgacccg cactcgaca atcgccccc 
      cagtttttc gtcagtctg tacgagctt atgaacaaa 
      atggagcag cggtcgtcc tttgcctgg aactgcgtc 
      gacgttttg gccaacatt tgccgcaac cccgcggcc 
      cgcagcgcc ctaatccac acctatcag ttcattccg 
      agtctgtcg cggctgttg agcgaccag ttatcaaac 
      gagaaaaaa atccggctg ttgagactc atgcaggac 
      ctcacttgc ggcatcaaa atctcgtgg caaatcccg 
      cacttgccc cagctcatg gtcacgctg agcaagtgg 
      atcgagacc tccaacgag gagataatc acgctctcg 
      atggggatt ttggtcaat ttgtgttac aaaaaccta 
      tcggcgatt tacacgctt ttcgggaac gtggacgta 
      aagaaaata ctccagatt tgttcgctg atgaggggc 
      ccaactatt gaaatacac acgtgcaaa atgttgatt 
      tttttggac tacttgaac ggcgatatt cccgaaaat 
      attctcctg aaaatcaca aatgtgact tttgagtcg 
      gttgttgaa gcttttcgg atacgagac tcgattttg 
      ttgcgacaa attgtcgaa tttttcctt gatgtgggc 
      aagcaaaac acgcttagt gttttagta caaagtcag 
      gactatagt aagcagctc acagccgtc ttggatatt 
      atacaaaat aatcacgga atggagacc ggcgacagc 
      actttcgag tgcgaaccc gaatgtttc gctttagtt 
      ttcgaattt ttgggtttt ttgacggaa atcaaagtc 
      ccggcagtg acttcaaag tacgctgat ttgatccaa 
      atcgcctta aagtggatt cacaccgac ttggtgtca 
      tcccaagct ttggctctt ttaaaatca attgccgta 
      aacgtggac gaaaacaaa atggagatt ttggagcct 
      ctcgttcag ggacttccc atttttttg aacactttg 
      gacagcagt cgtaacgaa gatggccct gcttacgtc 
      gaacagaat ttaaaaatc ggcggcttg ttacaactg 
      ttacgaatt atgacacaa gttgcgaca attcggcct 
      aaaattttg gaagatttg aaagttgca acggtttgt 
      aaagttttg gggtgtctc caagacgct gaaaacgtc 
      tcaaatagt aaaacaaac gacctggac atggccaac 
      attgacacg gttaattta tacgtccac ggaatagcc 
      ctagttaac gaattggcc aagcatgac atcacgtgg 
      atgaacctt cagaccaat ctcatgcag cacaagcaa 
      acccacatg ttgctagcg cagtgcctg taccaaagt 
      tcgaaagag gaaaaagcg ctagttttt gaaatgtcg 
      acaattcgg tgtttcccc cgaaagagc gtcgctgac 
      gcgatggtg gcgctccaa gcggcgaca gcgcccgac 
      cttaaaagc tcctcccac agcatccaa tcacaagac 
      ttgaatttc ccggttctt tcggttaca caaatcaac 
      aaagttgat tcgctcctc tccaaactg cgcgaaaac 
      ttcacccag aagcagttc ggttcggtt ctaacctct 
      gacgtcatc gagttgtac gagtacaag ttggcgtcg 
      atgggcaat gcagagcgc gctgcgatg gcaagtgtc 
      gaggcggcg tccgagcgc tgcacgcac ttgcagcac 
      aggaacgcc caattgagt gccgaatta aatcgctcg 
      catcaattg ttataccac gtgcaacag tgccacgag 
      caggtctcg aaattaacc gagtcgttg gaagaaaaa 
      gtcgaaact ttgaaaaag cagtcagag gttgaccga 
      ggcaagttg aaagtgtgt aaaagtcag ctgggggtg 
      aaggaggac gagctaaac gagtgcaaa gccgagctg 
      gagagttac aagaagaaa tatgagaag agtgtggcg 
      gtcaggagg gaggtggag gagcagaac gcccagttg 
      aagcaatac atcgccaaa ttggaggag aattcgacc 
      aagttgcag aaactggtg cagaagaag gaggaggcg 
      ctgaaaaac acgaatttg aaagtgagc gagttggag 
      caaggtttg acgcaaaaa gagaacgag ttgcaccag 
      gcgaaggcc gagctgcaa gaaaccacc aaagaatta 
      aatacccgg aaacaaatt ttagaaact atcacgaaa 
      ttcgccatg aactcgacg cgattggag caataattt 
      ttagatttt atctttata agagttgta taactgcca 
      gtactttta taattttac aataaaaaa ttggatgat 
      gc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY