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Gene information
General information
TC030652 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12746784..12751881
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030652
    iBeetle screen iB_09397

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030652 RA
      MGDCQLNYP PGEDRFCKY AYSSWCPTC ERSSPRRRS 
      RSETTECRR KCKEMKSLS PKKYARSRE YENMDLELN 
      NYNWYEPPR DYKIRSSQL CELPTSYIS NNCQTSKLP 
      PPEGVRNYE PALKMKQED FYDAFKYEK PKHHLNGFA 
      KKPPRSPYT VYDNQLSFD TSTYFNKIR DASNLHERY 
      IKEIESVRE RIALWKTGK KLEIEAKNQ KFVPVHGSK 
      LEQKYAKEL QAVKERLAS KNAKKSDVD DIITINQSE 
      IGPRPRKIA IKTKKINLG KMKKVRLPS SKINTPIAE 
      FTESDYSGS DESEAEEVL NADDESESL SEATCNGAE 
      PEIIPPQIP EGGDASGWP LRPSLFPHI PPYIRFSTH 
      DSDSPIEVP AGRRLFKWK LSTITPVVV RKTLTNSGF 
      ALVRKSNQW LGTWGKHMK SPMFKTLKE TQKLNHFPG 
      TFQLGRKDR LWRNFQRLI NKFGYKEFG FIPQTYVLP 
      HELKILKQN WEYKNGSGD AWIVKPPAS ARGAGIKVI 
      NKWSQLPKK MSLVVQKYI SNPYLINGS KFDLRLYVL 
      VTSFNPLRI YLYPDGLAR FASAKYSSD SKDLKDRFV 
      HLTNYSINK LSSQYTANE DANACQGHK WTITKLLEY 
      MSRQGVDTK ALWRNLQQL VIKTIITGE APIHQLCEE 
      NMLSRYNCY ELFGVDVLL DENLKAWLL EVNISPSLH 
      SASPLDAHV KGPLVQTLF DLAQFHFPP RLSQTVRHS 
      PQCFDPKLY TTSLTKKER EKHTLFEQY ESRHDYLDD 
      ILAELTGDD VRKLTQAED EWCVKGRFE RIFPTSQTH 
      KYLNFMEVR YYNRLFDAW ECRFEKNRQ EGINLLKAL 
      CAEKVHLKV APQSTLHKN SLSTSHGCA SLQPSREGQ 
      SRPLAEILQ EVTIESSSC N
    CDS sequence
    • >TC030652 RA
      atgggagat tgccaattg aattacccc cccggtgaa 
      gacagattt tgcaagtac gcgtattcg agttggtgc 
      cccacttgc gagcgcagc tccccgagg cggcgctcc 
      cgcagcgag accaccgaa tgccggcga aagtgcaag 
      gaaatgaag agtttgtcg ccgaaaaag tacgccagg 
      agccgcgag tacgagaac atggacttg gaattgaac 
      aattacaac tggtatgag ccccctcgc gattacaaa 
      atccggtca agccagctg tgcgaattg ccaacctct 
      tatatatcg aacaattgc caaacgtca aaattacca 
      ccaccagaa ggcgtcaga aattacgag cccgccctc 
      aaaatgaaa caagaagac ttttacgac gccttcaag 
      tacgagaaa ccgaaacac cacctcaac ggcttcgcc 
      aaaaagccc cccaggagt ccgtacacc gtctacgac 
      aatcagctc agtttcgac accagcacc tattttaat 
      aaaatccgc gatgcctca aacttgcac gagcgctac 
      attaaggag atcgagtcg gtgcgggag cgcattgcc 
      ctgtggaag acgggcaag aagctggaa atcgaagcc 
      aaaaaccaa aaatttgtg ccggtgcac ggcagcaag 
      ttggagcag aaatatgca aaggagctg caagctgtg 
      aaggagaga cttgcgtcc aaaaatgcc aaaaagtcg 
      gacgttgac gatattatt acgataaat cagagcgag 
      attggccca aggcccagg aaaattgcc atcaagacg 
      aagaaaatc aatctgggg aaaatgaag aaggtgcgc 
      ctgccttcc agcaagata aacacaccc atcgccgaa 
      ttcacggaa agcgactac agcggttct gacgaaagc 
      gaagccgaa gaagtgcta aacgccgac gacgaatcg 
      gaaagtctc agtgaggcc acgtgcaat ggcgccgaa 
      cccgaaatc atcccacca caaatacca gaaggtggg 
      gacgcctcg gggtggccc ctccgcccg agcctcttc 
      ccccacatc cccccatac atccgcttc agcacgcac 
      gactccgac agccccatt gaggtcccg gcaggtcgg 
      cgtttgttc aagtggaaa ttaagcaca ataacgccg 
      gttgtggtt cgcaaaact ttaacaaac tcgggcttt 
      gcgttggtc agaaagtcc aatcaatgg ttgggtacg 
      tggggcaaa cacatgaaa tcaccaatg tttaagacg 
      ttaaaagag acgcaaaaa ttgaatcat ttccctgga 
      acgtttcaa ttggggcgt aaagaccgg ctgtggcgc 
      aatttccaa agacttatc aataaattc ggatacaaa 
      gaatttggg ttcattcct caaacgtat gttctacct 
      cacgaattg aaaatactc aagcagaat tgggagtat 
      aagaacggc agtggggac gcgtggatt gttaaaccg 
      ccggcttca gctcgcggt gctggcatc aaagtgata 
      aacaagtgg tcccaactc cccaaaaaa atgtcccta 
      gttgtgcaa aaatacatt tcgaatccg tacctgata 
      aacgggagc aagtttgat ctcagattg tacgtcctg 
      gtcactagt ttcaatcca ttgcgcatt tacctgtat 
      ccagacggc ttggcgaga tttgcaagt gccaaatat 
      agttccgac tccaaggac ttgaaagac cgttttgtg 
      catttaacc aattacagt atcaataaa ttgtcgagt 
      cagtacaca gccaatgag gacgctaac gcgtgtcaa 
      ggccacaaa tggacgata acgaaacta ctcgaatac 
      atgtcgagg caaggtgtc gacacgaaa gccctctgg 
      cgcaattta caacaactc gtcatcaag acaatcatt 
      acgggggaa gcccccatc caccaattg tgcgaagaa 
      aacatgctc agtcggtac aattgctac gaattgttc 
      ggggtcgat gtcctactc gatgaaaat ctcaaagct 
      tggctgctg gaagtgaac atttcgccc agtttgcat 
      tcagccagc ccgctagat gcacatgtt aagggcccc 
      cttgtccaa accttattt gatctcgca cagttccat 
      tttccgcca aggttgtct caaaccgtc cgtcacagc 
      ccccaatgt ttcgacccc aaattatac acaacttcg 
      ctgacgaaa aaagagcgg gaaaagcac acacttttc 
      gaacagtat gagtcacga cacgactac ctcgacgat 
      attttggcc gaattgact ggcgatgat gtgcgaaag 
      ctgacgcaa gccgaagac gagtggtgt gttaagggg 
      cgtttcgag aggattttc cccacctcg cagacgcac 
      aagtatttg aatttcatg gaagttcgg tattataat 
      cggctgttt gatgcgtgg gagtgccga tttgagaag 
      aacaggcag gaagggatt aacttattg aaggcgtta 
      tgcgccgaa aaagtgcac ttaaaagtg gcgccgcag 
      agtacgcta cataagaat tcgctgagt acgagccat 
      gggtgtgcg agtctgcag ccgtcgagg gaaggccag 
      agtcggcct cttgcagaa atcctgcaa gaggtcaca 
      atcgaaagc tctagctgt aattaa
    mRNA sequence
    • >TC030652 RA
      cctgtgtta gaaaaggaa aatgaaaag aacgtaatc 
      tgagctagt cccggcggg cgtaattgt gaactacac 
      gcgggcaaa aatggtacg gttcctcga cctgtcttg 
      aagaaattt taatggcaa tttccctca gaaaatgct 
      aattggagt ttattgatt ttcccaaaa atagccccc 
      tgcgatggg agattgcca attgaatta cccccccgg 
      tgaagacag attttgcaa gtacgcgta ttcgagttg 
      gtgccccac ttgcgagcg cagctcccc gaggcggcg 
      ctcccgcag cgagaccac cgaatgccg gcgaaagtg 
      caaggaaat gaagagttt gtcgccgaa aaagtacgc 
      caggagccg cgagtacga gaacatgga cttggaatt 
      gaacaatta caactggta tgagccccc tcgcgatta 
      caaaatccg gtcaagcca gctgtgcga attgccaac 
      ctcttatat atcgaacaa ttgccaaac gtcaaaatt 
      accaccacc agaaggcgt cagaaatta cgagcccgc 
      cctcaaaat gaaacaaga agactttta cgacgcctt 
      caagtacga gaaaccgaa acaccacct caacggctt 
      cgccaaaaa gccccccag gagtccgta caccgtcta 
      cgacaatca gctcagttt cgacaccag cacctattt 
      taataaaat ccgcgatgc ctcaaactt gcacgagcg 
      ctacattaa ggagatcga gtcggtgcg ggagcgcat 
      tgccctgtg gaagacggg caagaagct ggaaatcga 
      agccaaaaa ccaaaaatt tgtgccggt gcacggcag 
      caagttgga gcagaaata tgcaaagga gctgcaagc 
      tgtgaagga gagacttgc gtccaaaaa tgccaaaaa 
      gtcggacgt tgacgatat tattacgat aaatcagag 
      cgagattgg cccaaggcc caggaaaat tgccatcaa 
      gacgaagaa aatcaatct ggggaaaat gaagaaggt 
      gcgcctgcc ttccagcaa gataaacac acccatcgc 
      cgaattcac ggaaagcga ctacagcgg ttctgacga 
      aagcgaagc cgaagaagt gctaaacgc cgacgacga 
      atcggaaag tctcagtga ggccacgtg caatggcgc 
      cgaacccga aatcatccc accacaaat accagaagg 
      tggggacgc ctcggggtg gcccctccg cccgagcct 
      cttccccca catcccccc atacatccg cttcagcac 
      gcacgactc cgacagccc cattgaggt cccggcagg 
      tcggcgttt gttcaagtg gaaattaag cacaataac 
      gccggttgt ggttcgcaa aactttaac aaactcggg 
      ctttgcgtt ggtcagaaa gtccaatca atggttggg 
      tacgtgggg caaacacat gaaatcacc aatgtttaa 
      gacgttaaa agagacgca aaaattgaa tcatttccc 
      tggaacgtt tcaattggg gcgtaaaga ccggctgtg 
      gcgcaattt ccaaagact tatcaataa attcggata 
      caaagaatt tgggttcat tcctcaaac gtatgttct 
      acctcacga attgaaaat actcaagca gaattggga 
      gtataagaa cggcagtgg ggacgcgtg gattgttaa 
      accgccggc ttcagctcg cggtgctgg catcaaagt 
      gataaacaa gtggtccca actccccaa aaaaatgtc 
      cctagttgt gcaaaaata catttcgaa tccgtacct 
      gataaacgg gagcaagtt tgatctcag attgtacgt 
      cctggtcac tagtttcaa tccattgcg catttacct 
      gtatccaga cggcttggc gagatttgc aagtgccaa 
      atatagttc cgactccaa ggacttgaa agaccgttt 
      tgtgcattt aaccaatta cagtatcaa taaattgtc 
      gagtcagta cacagccaa tgaggacgc taacgcgtg 
      tcaaggcca caaatggac gataacgaa actactcga 
      atacatgtc gaggcaagg tgtcgacac gaaagccct 
      ctggcgcaa tttacaaca actcgtcat caagacaat 
      cattacggg ggaagcccc catccacca attgtgcga 
      agaaaacat gctcagtcg gtacaattg ctacgaatt 
      gttcggggt cgatgtcct actcgatga aaatctcaa 
      agcttggct gctggaagt gaacatttc gcccagttt 
      gcattcagc cagcccgct agatgcaca tgttaaggg 
      cccccttgt ccaaacctt atttgatct cgcacagtt 
      ccattttcc gccaaggtt gtctcaaac cgtccgtca 
      cagccccca atgtttcga ccccaaatt atacacaac 
      ttcgctgac gaaaaaaga gcgggaaaa gcacacact 
      tttcgaaca gtatgagtc acgacacga ctacctcga 
      cgatatttt ggccgaatt gactggcga tgatgtgcg 
      aaagctgac gcaagccga agacgagtg gtgtgttaa 
      ggggcgttt cgagaggat tttccccac ctcgcagac 
      gcacaagta tttgaattt catggaagt tcggtatta 
      taatcggct gtttgatgc gtgggagtg ccgatttga 
      gaagaacag gcaggaagg gattaactt attgaaggc 
      gttatgcgc cgaaaaagt gcacttaaa agtggcgcc 
      gcagagtac gctacataa gaattcgct gagtacgag 
      ccatgggtg tgcgagtct gcagccgtc gagggaagg 
      ccagagtcg gcctcttgc agaaatcct gcaagaggt 
      cacaatcga aagctctag ctgtaatta actgtataa 
      gtgagttgt ttgttgcgg tttttcccg ttctaatcg 
      agtggcggt ttcagatgg aattctaga agtgtgttt 
      cttttgtta cgaatgagg acaaacacc agcatttaa 
      actgccaaa tttgtacgt tgttttaat tccttgtta 
      aattattgt agcgagata ttccattta catttgcat 
      ttacagcaa agtgttttt taattgatt tttattagt 
      gtattgagt ctgatgaat aaaatattt ctttacatt 
      aaatgtctt cgtctgggt ccgggaccg cgttcctcg 
      ggatctcaa tcctctgga ctgtgacgt tgagttgca 
      tccttatat ccagcaaaa agcacagaa acaggatga 
      agaggggca ccttgaggc gcagggtgt agcccagct 
      tgagcagaa aaagcaaag gttcggcga gtggcatga 
      ctcagcacc accacgatc acattttta caaagaaac 
      gcccaaatt ttggcaaaa tattttgaa attggttga 
      catttgtca gcgacaggt gtcagttcg ccagcc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY