General information | |||
TC034159
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007423.3: 12696149..12707646 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC034159 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_09395 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
remarks: muscle pattern potentially obscured by segmentation defect,
embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 50% - 80%
head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
mandible not present - Penetrance (percentage of animals/eggs on slide): less than 30%
thorax segment not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
thorax segment partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
legs tibiotarsus potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
legs femur potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
legs swollen - Penetrance (percentage of animals/eggs on slide): less than 30%
abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
posterior terminus structure irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC034159 RA
MEGDSTNRE GAPLGDSGS SSLTLSLTP PLSETSTIS EHLSSVEND RSLPELLVN FPPDERISL EPKPPSLSD SYTSSFVSL NGLERFIST NMEGRTHAQ SILEEILSN DQFKSDSDE SLNIFANVD LDDKLLNSY NAGKSVNFD LNDEVCKRV KDFEDLIAV KNTTIAALT SELDSFREL LSNTSSLGT TTTEYKQFQ EECHNKLVE YRDAIVHRD EQIEKLTSS LQQAILNRE EMKQHFRSE IAQLQEQLE KTSNLLKDH KKSFDFDEI CAQFEHSLG PPQLAPFGE LKAAFASYV EMSKRALEN EISNLKTKF SSDKAQCES EIKSELDAK HSKEMEELR TYFEKKCAD LEKNYSEEV FSQQSRKMS ESSSCSELS SDFVGPGGD TRVDYTRKD LVKLRQSLV QLIKIFDGF NLEDLSDSD FERLTSEIG KFEFRNLLK FDLSLMKSE LQNKYHAEL EILREDYDN RVDELNVEH EKKLKSLNE EIDDLNRQL RGRIVTTSQ QEVANSGEF EIDEVVQSY ERRLQEQVT LAKIDIIAA LELQIQRLV ASDSLDDDW PEELLQLKS RFADKYQTE IAALKQQHS EEIARLKDE HLKNLNAAL ERARRRSLR DADSLSKGE LELLKERDL LKKQTLALR NLLGELIKY FTQCEDELN NTLVEELMT KNFTQIERE LDSLENTSG VKRVHLTPN FTELIDLID NSPDKDLET IDLKSELGS CLEKLKADA NAILQLSTS FAKSEELEV PKRKNESLE LTRKLINET QIKNELVDQ LSEARSIIH SLETDRGAL ENQLEQLIE RQKNLECDL LTARDKIAE LIENGHKEI VSEGYGENG DRGVRGLAE AVAALSVLQ DEARVLVEN RSQVDQNVV KLVEGLARA GDKIVEEAR REREDLKQQ IEAADKKYK ITCQFLEDQ AAEREQERD DAQKQIDSL KDHLRDREK DRATYERYT TEVEHLEQQ LRDMSKLVE VGEKKVKEV EGERQEAVE KIYELRDII RDLEEQVRV KTSTEEELR SIVSELEII VKQQVTDEK KDVSENTKE FHQYVGDLE NEVQRLRLT SEIAGSEGA LKQIKNQLS DFETTLDKR TKDLEGFHS TVSTTCSSP SEDMSIQER SIYDECEIP LQQLARLKE KLLRHSRAE DAALKRIRD LEMQIVALK NDLEESQTE REILRKQIS DHLVLISNL QIRLDEQRI RAEHIEKQT NTSLEVQNY DLKNTIASL EEKIAKRDK TIAQLKSNI DETKKRLEE REKEDDLVV QMQEQVEFL RSENAKLKE ESKLLNLDE KEVESLNRL LLSGVSIHD LENFEQCRR QMTDSFPIS PIPGRKNHE TTFLSLPNK ANSTEKHVR FEDDQLKQL EGKIELLQT HLDETEENL KKATQLFEE EQKKSEEQI KLLEAKNGE IEANFRKLL QEEKTKSEE IQARLDQAN DEMKKSKEL EANLRLQNE EIQARLDQG TEEMKKSEA KLQECSRRL ESRDKEVKT LYKVNNELK NSVKIKEEE IAEIPLLRK RNEEVGEEL RHLQHLLLE KEKIIEHME ADSKSLHTN LETIQNKLQ ETGNVVDLS RRLREEQKK NAELFEELH TLKAQLLSV DKKSLEDIT SQIERELNY SAYLDANLL NAVGEGSLE SEDVDGLKL LLKKQKSVN KQLVKAREA VETKFELLE QKYENLKKE LERVQLEDA VLIGQLRTK LTQTMDVED EALRDLRES RKYELGLKN EKIIDLESQ ISAFEHNER LLRADLEAK VNELGACRV LQEQSKAEI RKLKRLLSE HKAKSSDLV PDQIMEKIK ELNAVARDN KQMIDLIAR LNNEKLVLE ERIVALEEH HNYLPFDDP SERANYLFA KYLRSESYR KALVWQKKY LISLIEMVY VTPVEHSIV PKRAKFKHV GIMVVSICR MKYLVKRWH SGKRIVRRK SRETVNFQV GQPVSSQNF VPSALNQPK TDMPWVGTS PPTKEPQVL RFQRIATRD ALSSPGLLS KYVERFNQI QDKLGLDTQ
- >TC034159 RA
atggagggc gattcgacc aatcgggag ggtgccccc cttggagac tccggtagt agcagcttg actctgtcc ctaaccccg cctttgtcc gagacaagc acgatctcc gagcaccta tcaagcgtc gaaaacgac cgttcgttg ccagagctg ctcgtcaat ttcccccca gatgagagg atctcactg gagcccaaa ccgccatcc cttagtgat agttacacg agtagtttc gttagtcta aacgggcta gagcgcttc attagtaca aacatggag gggcggaca cacgctcaa agcatcctc gaggaaatc ctaagtaac gaccagttc aagtccgat tccgacgaa agcttgaac attttcgcc aacgtggac ctcgacgac aaactccta aacagttac aacgccggc aaaagcgtc aatttcgac ctaaacgac gaggtttgc aaacgagtc aaagacttt gaggatttg atcgcagtc aaaaacacc actattgcc gccctaact tccgaactc gactcattt cgcgaattg ttgagcaac acgtcgagt ctggggacc accaccacc gagtacaaa cagttccaa gaggaatgc cataataag ttagtcgag taccgcgac gctattgta catagagac gaacaaatc gaaaaattg accagttcg ttacaacag gcgatttta aatcgcgaa gaaatgaaa caacacttt aggagcgaa atcgcgcag ttgcaagaa cagctggaa aaaacctca aacttgctc aaggaccac aaaaaatcg tttgacttt gacgagatt tgcgcgcag tttgagcac agtttgggg cccccacag ttggcgcct ttcggcgag ttgaaggcg gcgttcgct agttacgtg gagatgagc aaaagggcg ctcgagaac gagattagc aatctgaag accaagttt agttcggat aaagcccag tgtgagagc gagattaag agcgagttg gacgccaaa cattcgaag gaaatggag gagttgcgg acctacttt gagaagaaa tgcgccgat ttggagaaa aattactcg gaggaggtg ttcagtcag cagagccgc aaaatgtcc gaatcgagt tcgtgttcc gagctcagt tcggacttt gtggggcca gggggcgac actagagtt gactacaca aggaaggat ttggttaaa ttgcgccag agtttggtt cagttgatt aaaattttc gatggtttc aatttggag gatttgtcc gattccgat tttgagcgt ctcacgtcc gaaattggc aaatttgag tttcggaat ttgctcaag ttcgattta agtctcatg aagagtgag ctgcagaat aagtatcac gctgaattg gagattttg agggaggat tacgataat cgtgtcgat gagttaaac gttgagcat gagaagaaa ttgaagagt ttgaacgag gaaattgac gatcttaat cggcagttg aggggtcgg attgttacg actagtcaa caggaagtt gcgaattcg ggcgagttt gagattgat gaagtggtg caaagctat gagagacgg ttgcaagaa caagtcact ctagctaaa attgacatc attgctgcg ttagaattg cagatacag cgacttgtc gcaagcgac tcacttgac gacgactgg cctgaagag ctcctccag ctcaaatca cgttttgcc gacaaatac cagaccgaa attgccgca ctcaagcag caacactcg gaggaaatc gctcgtttg aaagacgaa cacttgaaa aacctcaac gctgcttta gaacgtgca cgcagacgt agtcttcga gatgcggac agtttgagt aaaggcgaa cttgaatta ctcaaagaa cgcgattta ctcaaaaaa caaactctg gcgttgcgc aatttattg ggcgagttg attaaatat ttcacacag tgtgaggat gagctaaat aacactttg gtcgaggag ctcatgacg aaaaatttc acacaaatt gagagagaa ttagactcg cttgagaac accagtggc gttaaaagg gttcatctg acaccgaat tttaccgaa ttgattgat ttgatcgac aattcgccc gataaggat ttggaaacg attgattta aagagcgag cttggttcc tgtttggag aaactgaaa gccgacgcc aatgccatt ctacagttg agcacaagt tttgcaaaa agtgaggaa cttgaggtt ccaaaacgt aaaaacgag tcgttggaa ttaacccgg aaattgatc aacgaaaca cagattaaa aacgaattg gttgatcaa ttatcagag gccagaagt ataattcat tcgcttgag acagacaga ggggcgctt gaaaatcaa ctggaacag ctcatcgaa cggcaaaaa aatctcgaa tgtgatttg ctaacagcg cgtgataaa atcgccgaa ttgatcgaa aatgggcac aaggaaatt gtgtcggaa ggttacggt gaaaatggg gatcgtggt gttaggggt ttggctgaa gctgttgcc gcattgagt gtgttacaa gatgaggca agagttttg gtggaaaat cgcagtcaa gtggatcaa aatgtggtc aagttggtg gaggggttg gccagggct ggggataaa atcgtggag gaggcgagg agggagcgg gaggatctg aaacagcag atcgaggcc gccgacaag aaatacaaa ataacttgt caatttttg gaggatcaa gccgccgaa cgcgaacaa gaacgcgac gatgcccag aagcaaatc gattcatta aaagaccat ttaagagac agggaaaag gatagggcc acctatgaa cgttacacc accgaggtc gaacatctg gaacaacaa ctgcgtgat atgtcaaag ttagtcgaa gttggcgag aagaaagtc aaagaagtg gaaggcgag cgacaagaa gccgttgag aaaatctac gagttgagg gacattatc cgggacttg gaggaacaa gtcagagtt aaaacctca accgaggag gaattgagg tctatagtg agcgagttg gaaataatc gtgaaacaa caagtcacc gacgagaaa aaagacgtt tcggaaaac acgaaagag tttcatcag tacgttggc gatttggag aatgaagtt caaaggtta cgactgacc agtgagatt gcgggcagt gaaggcgcc ctgaaacaa atcaaaaat caattgagc gattttgaa acaactctc gataagcga acgaaggat ttggaaggt ttccattca acagtttcc acaacgtgt agttccccg agcgaagat atgtcaata caagaacgc tcgatttac gacgaatgc gaaattccc ctccagcag ttggcacgc ctgaaggaa aagttgttg aggcattcg agggccgaa gatgccgcg cttaaacgc atccgcgac ctcgaaatg caaattgtg gccctcaaa aacgatcta gaggagtcg caaaccgag cgcgaaatt ttgagaaaa caaatctcc gatcacttg gtcctaatt tcaaatctc caaatacgt ctcgacgaa caacgaatt cgtgccgaa cacattgag aaacaaaca aacacatcg ttggaagtg caaaactac gatttgaaa aatacaatc gcaagtttg gaggaaaaa attgccaag cgggacaaa acaatagcg cagctaaaa tcaaacatt gacgaaacg aaaaaacgt ctcgaagag cgtgaaaaa gaggacgat ctcgttgta caaatgcag gaacaagtc gagttttta cgctccgaa aatgcaaaa ttgaaagaa gaaagtaaa ttattgaat ttggacgag aaagaagtc gagtctttg aatcgtttg cttttgtcg ggcgtttcg attcacgat ttggagaat ttcgaacaa tgtagacga caaatgacc gattcgttc cccatatca ccaatcccc ggacgaaaa aatcacgaa actacgttt ttgtcgttg ccgaataaa gcaaactcg accgaaaaa cacgtccgt tttgaagac gaccaattg aagcaactt gaaggcaaa attgagctt ttgcaaacg catttggac gaaactgag gagaatttg aagaaagcc actcaattg tttgaagaa gaacagaag aaaagtgag gagcaaatc aagttactt gaggccaag aatggggaa attgaggcc aatttccgg aaattgctt caagaagaa aagaccaaa agtgaagaa atccaggcc cgacttgac caagcaaac gacgaaatg aagaaaagt aaggaactt gaggccaat ttgcggttg caaaacgaa gagatccaa gccaggctt gaccaaggc acggaagaa atgaagaaa agcgaagca aagttacag gagtgtagc agaagactc gaatcgcgg gacaaagaa gtgaaaact ttgtacaaa gtgaacaac gaattgaag aacagtgtg aagataaaa gaggaggaa attgccgaa attcccctt ttgaggaag aggaatgaa gaagtgggc gaagagttg cgtcacttg caacacctc ctcttggaa aaggagaaa ataatcgag catatggag gctgacagt aaaagtctt cacacgaat ttggaaacg attcaaaat aaattgcaa gagacggga aacgtggtc gatctcagt cggcgtttg cgcgaagaa cagaagaaa aacgccgaa ttgtttgag gagttgcac acgctaaaa gcccagctt ttgagtgtt gataaaaaa tcgctcgag gatattacg agtcaaatt gagcgcgag ttaaattac tcggcttat ttggatgcg aatttgttg aatgcggtc ggtgagggc tctcttgaa agtgaagat gtggacggc ttaaaacta ctattgaaa aaacagaaa tctgtaaac aaacaattg gtgaaagcc cgtgaagcg gtcgaaaca aaattcgaa cttttggaa caaaaatac gaaaatttg aaaaaggag ctggaacgc gtccaatta gaggacgca gttttaatc ggccagttg cggacgaag ttgactcaa acgatggac gttgaggac gaggctttg agggacttg agggagagt agaaagtac gaactgggc ttgaaaaac gagaaaata atcgatttg gagtcgcaa atttcggcg tttgagcac aacgagcga cttttgagg gccgatttg gaagcgaaa gtcaacgaa ttgggtgcg tgtagagtg ttgcaggag caaagtaag gccgaaatt cgcaaatta aagcgcctg ctgtcggag cataaagca aaatcgagt gacttagtc ccggaccaa atcatggag aaaatcaaa gaactgaac gccgtggcg cgcgataac aagcaaatg attgattta atcgcgcgt ttgaacaac gagaagttg gttttggag gagagaatc gttgcgttg gaggagcac cacaattat ttgccgttt gatgatccg tccgaacgt gcgaattat ctctttgcc aagtatttg cggtccgag agttaccgt aaagcgttg gtgtggcag aaaaaatac ctaatatcg ctcattgaa atggtttat gtcactccg gttgagcac tcgattgtt ccaaagcgg gccaagttc aagcatgtt ggtattatg gttgtgtcg atttgtcgt atgaaatat ttggttaaa aggtggcat tcgggcaaa cgaatcgtt cggagaaaa agccgagaa acggttaat ttccaagtt ggtcagcct gtgtcaagt cagaatttc gttcctagt gccttaaac cagcccaag acggatatg ccatgggtg gggacgtcg cccccgact aaagagccc caagttttg aggtttcaa aggatagcg acaagggat gcgttaagt agtcctggg ctcttgtcc aaatacgtg gaacggttc aatcaaatc caggataag ttggggttg gacactcag tga
- >TC034159 RA
aaaattttc aaattgttt gctttttga gtgccgtac ttcgtgtca ttttgtaca attaggtgc catttcgtc ccaattaaa ttaaataaa aattctaac gtcccattt tcccaattt gtcgccaaa ccaatcaat caagtcgcc tataacaac aacaaacaa actcgaaaa tgtcacact tgacgtttg ttgcaattt tatttattg tttaatggg ttaaaataa acctttgtg gcataatgg agggcgatt cgaccaatc gggagggtg ccccccttg gagactccg gtagtagca gcttgactc tgtccctaa ccccgcctt tgtccgaga caagcacga tctccgagc acctatcaa gcgtcgaaa acgaccgtt cgttgccag agctgctcg tcaatttcc ccccagatg agaggatct cactggagc ccaaaccgc catccctta gtgatagtt acacgagta gtttcgtta gtctaaacg ggctagagc gcttcatta gtacaaaca tggaggggc ggacacacg ctcaaagca tcctcgagg aaatcctaa gtaacgacc agttcaagt ccgattccg acgaaagct tgaacattt tcgccaacg tggacctcg acgacaaac tcctaaaca gttacaacg ccggcaaaa gcgtcaatt tcgacctaa acgacgagg tttgcaaac gagtcaaag actttgagg atttgatcg cagtcaaaa acaccacta ttgccgccc taacttccg aactcgact catttcgcg aattgttga gcaacacgt cgagtctgg ggaccacca ccaccgagt acaaacagt tccaagagg aatgccata ataagttag tcgagtacc gcgacgcta ttgtacata gagacgaac aaatcgaaa aattgacca gttcgttac aacaggcga ttttaaatc gcgaagaaa tgaaacaac actttagga gcgaaatcg cgcagttgc aagaacagc tggaaaaaa cctcaaact tgctcaagg accacaaaa aatcgtttg actttgacg agatttgcg cgcagtttg agcacagtt tggggcccc cacagttgg cgcctttcg gcgagttga aggcggcgt tcgctagtt acgtggaga tgagcaaaa gggcgctcg agaacgaga ttagcaatc tgaagacca agtttagtt cggataaag cccagtgtg agagcgaga ttaagagcg agttggacg ccaaacatt cgaaggaaa tggaggagt tgcggacct actttgaga agaaatgcg ccgatttgg agaaaaatt actcggagg aggtgttca gtcagcaga gccgcaaaa tgtccgaat cgagttcgt gttccgagc tcagttcgg actttgtgg ggccagggg gcgacacta gagttgact acacaagga aggatttgg ttaaattgc gccagagtt tggttcagt tgattaaaa ttttcgatg gtttcaatt tggaggatt tgtccgatt ccgattttg agcgtctca cgtccgaaa ttggcaaat ttgagtttc ggaatttgc tcaagttcg atttaagtc tcatgaaga gtgagctgc agaataagt atcacgctg aattggaga ttttgaggg aggattacg ataatcgtg tcgatgagt taaacgttg agcatgaga agaaattga agagtttga acgaggaaa ttgacgatc ttaatcggc agttgaggg gtcggattg ttacgacta gtcaacagg aagttgcga attcgggcg agtttgaga ttgatgaag tggtgcaaa gctatgaga gacggttgc aagaacaag tcactctag ctaaaattg acatcattg ctgcgttag aattgcaga tacagcgac ttgtcgcaa gcgactcac ttgacgacg actggcctg aagagctcc tccagctca aatcacgtt ttgccgaca aataccaga ccgaaattg ccgcactca agcagcaac actcggagg aaatcgctc gtttgaaag acgaacact tgaaaaacc tcaacgctg ctttagaac gtgcacgca gacgtagtc ttcgagatg cggacagtt tgagtaaag gcgaacttg aattactca aagaacgcg atttactca aaaaacaaa ctctggcgt tgcgcaatt tattgggcg agttgatta aatatttca cacagtgtg aggatgagc taaataaca ctttggtcg aggagctca tgacgaaaa atttcacac aaattgaga gagaattag actcgcttg agaacacca gtggcgtta aaagggttc atctgacac cgaatttta ccgaattga ttgatttga tcgacaatt cgcccgata aggatttgg aaacgattg atttaaaga gcgagcttg gttcctgtt tggagaaac tgaaagccg acgccaatg ccattctac agttgagca caagttttg caaaaagtg aggaacttg aggttccaa aacgtaaaa acgagtcgt tggaattaa cccggaaat tgatcaacg aaacacaga ttaaaaacg aattggttg atcaattat cagaggcca gaagtataa ttcattcgc ttgagacag acagagggg cgcttgaaa atcaactgg aacagctca tcgaacggc aaaaaaatc tcgaatgtg atttgctaa cagcgcgtg ataaaatcg ccgaattga tcgaaaatg ggcacaagg aaattgtgt cggaaggtt acggtgaaa atggggatc gtggtgtta ggggtttgg ctgaagctg ttgccgcat tgagtgtgt tacaagatg aggcaagag ttttggtgg aaaatcgca gtcaagtgg atcaaaatg tggtcaagt tggtggagg ggttggcca gggctgggg ataaaatcg tggaggagg cgaggaggg agcgggagg atctgaaac agcagatcg aggccgccg acaagaaat acaaaataa cttgtcaat ttttggagg atcaagccg ccgaacgcg aacaagaac gcgacgatg cccagaagc aaatcgatt cattaaaag accatttaa gagacaggg aaaaggata gggccacct atgaacgtt acaccaccg aggtcgaac atctggaac aacaactgc gtgatatgt caaagttag tcgaagttg gcgagaaga aagtcaaag aagtggaag gcgagcgac aagaagccg ttgagaaaa tctacgagt tgagggaca ttatccggg acttggagg aacaagtca gagttaaaa cctcaaccg aggaggaat tgaggtcta tagtgagcg agttggaaa taatcgtga aacaacaag tcaccgacg agaaaaaag acgtttcgg aaaacacga aagagtttc atcagtacg ttggcgatt tggagaatg aagttcaaa ggttacgac tgaccagtg agattgcgg gcagtgaag gcgccctga aacaaatca aaaatcaat tgagcgatt ttgaaacaa ctctcgata agcgaacga aggatttgg aaggtttcc attcaacag tttccacaa cgtgtagtt ccccgagcg aagatatgt caatacaag aacgctcga tttacgacg aatgcgaaa ttcccctcc agcagttgg cacgcctga aggaaaagt tgttgaggc attcgaggg ccgaagatg ccgcgctta aacgcatcc gcgacctcg aaatgcaaa ttgtggccc tcaaaaacg atctagagg agtcgcaaa ccgagcgcg aaattttga gaaaacaaa tctccgatc acttggtcc taatttcaa atctccaaa tacgtctcg acgaacaac gaattcgtg ccgaacaca ttgagaaac aaacaaaca catcgttgg aagtgcaaa actacgatt tgaaaaata caatcgcaa gtttggagg aaaaaattg ccaagcggg acaaaacaa tagcgcagc taaaatcaa acattgacg aaacgaaaa aacgtctcg aagagcgtg aaaaagagg acgatctcg ttgtacaaa tgcaggaac aagtcgagt ttttacgct ccgaaaatg caaaattga aagaagaaa gtaaattat tgaatttgg acgagaaag aagtcgagt ctttgaatc gtttgcttt tgtcgggcg tttcgattc acgatttgg agaatttcg aacaatgta gacgacaaa tgaccgatt cgttcccca tatcaccaa tccccggac gaaaaaatc acgaaacta cgtttttgt cgttgccga ataaagcaa actcgaccg aaaaacacg tccgttttg aagacgacc aattgaagc aacttgaag gcaaaattg agcttttgc aaacgcatt tggacgaaa ctgaggaga atttgaaga aagccactc aattgtttg aagaagaac agaagaaaa gtgaggagc aaatcaagt tacttgagg ccaagaatg gggaaattg aggccaatt tccggaaat tgcttcaag aagaaaaga ccaaaagtg aagaaatcc aggcccgac ttgaccaag caaacgacg aaatgaaga aaagtaagg aacttgagg ccaatttgc ggttgcaaa acgaagaga tccaagcca ggcttgacc aaggcacgg aagaaatga agaaaagcg aagcaaagt tacaggagt gtagcagaa gactcgaat cgcgggaca aagaagtga aaactttgt acaaagtga acaacgaat tgaagaaca gtgtgaaga taaaagagg aggaaattg ccgaaattc cccttttga ggaagagga atgaagaag tgggcgaag agttgcgtc acttgcaac acctcctct tggaaaagg agaaaataa tcgagcata tggaggctg acagtaaaa gtcttcaca cgaatttgg aaacgattc aaaataaat tgcaagaga cgggaaacg tggtcgatc tcagtcggc gtttgcgcg aagaacaga agaaaaacg ccgaattgt ttgaggagt tgcacacgc taaaagccc agcttttga gtgttgata aaaaatcgc tcgaggata ttacgagtc aaattgagc gcgagttaa attactcgg cttatttgg atgcgaatt tgttgaatg cggtcggtg agggctctc ttgaaagtg aagatgtgg acggcttaa aactactat tgaaaaaac agaaatctg taaacaaac aattggtga aagcccgtg aagcggtcg aaacaaaat tcgaacttt tggaacaaa aatacgaaa atttgaaaa aggagctgg aacgcgtcc aattagagg acgcagttt taatcggcc agttgcgga cgaagttga ctcaaacga tggacgttg aggacgagg ctttgaggg acttgaggg agagtagaa agtacgaac tgggcttga aaaacgaga aaataatcg atttggagt cgcaaattt cggcgtttg agcacaacg agcgacttt tgagggccg atttggaag cgaaagtca acgaattgg gtgcgtgta gagtgttgc aggagcaaa gtaaggccg aaattcgca aattaaagc gcctgctgt cggagcata aagcaaaat cgagtgact tagtcccgg accaaatca tggagaaaa tcaaagaac tgaacgccg tggcgcgcg ataacaagc aaatgattg atttaatcg cgcgtttga acaacgaga agttggttt tggaggaga gaatcgttg cgttggagg agcaccaca attatttgc cgtttgatg atccgtccg aacgtgcga attatctct ttgccaagt atttgcggt ccgagagtt accgtaaag cgttggtgt ggcagaaaa aatacctaa tatcgctca ttgaaatgg tttatgtca ctccggttg agcactcga ttgttccaa agcgggcca agttcaagc atgttggta ttatggttg tgtcgattt gtcgtatga aatatttgg ttaaaaggt ggcattcgg gcaaacgaa tcgttcgga gaaaaagcc gagaaacgg ttaatttcc aagttggtc agcctgtgt caagtcaga atttcgttc ctagtgcct taaaccagc ccaagacgg atatgccat gggtgggga cgtcgcccc cgactaaag agccccaag ttttgaggt ttcaaagga tagcgacaa gggatgcgt taagtagtc ctgggctct tgtccaaat acgtggaac ggttcaatc aaatccagg ataagttgg ggttggaca ctcagtgat tgattgtgt ctttaacga agtctagtc attgattta gccgaatga cattgtgcc ttgcctttc agcaacgaa gtgagatgt attttgatt aagtgtaaa gttaggttt aagagaatt ttgtaattg tttgttttt gcactttaa acgatttca gataaattc ttgtatata ttttaacga tttcaacaa aaatcggcg agattatcg acagcgtg
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
