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Gene information
General information
TC034140 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12078231..12084223
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034140
    iBeetle screen iB_09393

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034140 RA
      MVKSETRVN LPWVEKYRP SSLDDLVSH DDIIKTIGK 
      FIREDQVPH LLFYGPPGT GKTSTILAC ARQLYTPAQ 
      FSSMVLELN ASDDRGIGI VRGQILTFA STRTIFKSG 
      FKLIILDEA DAMTMDAQN ALRRIIEKY TENVRFCII 
      CNYLSKIIP ALQSRCTRF RFAPLAPEQ ILPRLNYVI 
      DEEKVKVTD DGKQALLTL ANGDMRKVL NVLQSTWLA 
      YQNVTEDNV YTCVGHPLK NDIESIVKW LLNQDFQTA 
      FKNIKELKG VKGLALNDI ITEVHRYVH RIEFPLEIM 
      LSLLNQLAE IEYRLVQGA NENIQLTAL IAAFHKAKE 
      ASPPETV
    • >TC034140 RB
      MVKSETRVN LPWVEKYRP SSLDDLVSH DDIIKTIGK 
      FIREDQVPH LLFYGPPGT GKTSTILAC ARQLYTPAQ 
      FSSMVLELN ASDDRGIGI VRGQILTFA STRTIFKSG 
      FKLIILDEA DAMTMDAQN ALRRSK
    • >TC034140 RC
      MVKSETRVN LPWVEKYRP SSLDDLVSH DDIIKTIGK 
      FIREDQVPH LLFYGPPGT GKTSTILAC ARQLYTPAQ 
      FSSMVLELN ASDDRGIGI VRGQILTFA STRTIFKSG 
      FKLIILDEA DAMTMDAQN ALRRTPYYL IAIYQL
    CDS sequence
    • >TC034140 RA
      atggttaaa agcgagaca cgcgtcaat ttgccttgg 
      gtcgaaaaa taccgcccc agcagtctg gacgacttg 
      gtgtctcac gacgatatt atcaagaca atcggtaaa 
      tttattagg gaggaccag gtcccccac ttgttgttc 
      tatggcccc cctggaacg ggcaaaact agcactatt 
      ctggcttgc gcccgccag ctttacacc ccggcccag 
      ttttcctca atggttttg gaattgaat gcctctgat 
      gatcggggt attggtatt gtaagggga caaatcctc 
      acgtttgcg agcacacgc acaattttc aagtcaggc 
      ttcaagctg attattttg gacgaagcg gacgcaatg 
      accatggac gcccaaaac gccctcagg agaatcatc 
      gagaagtac acggaaaac gtgcgcttt tgcataatt 
      tgcaattac ttgagtaag attattccg gccctacag 
      tcacgctgt acgaggttt aggttcgcc ccactggcc 
      cccgaacag attctgcca cgtttgaat tatgtcatc 
      gatgaggag aaggttaag gtgacagac gatggcaaa 
      caagcgctt ctcactttg gcaaacggc gacatgcgg 
      aaagtgtta aatgtgctc cagagcacg tggttggct 
      tatcaaaac gtgacggag gataacgtc tacacgtgc 
      gtgggacac cctttgaag aacgatatt gaaagtatt 
      gtcaaatgg ctacttaac caggacttc caaactgcg 
      tttaaaaat attaaggaa ctgaagggg gttaaaggc 
      ttggctttg aatgatatc attacggaa gtccaccgt 
      tacgttcat agaattgaa tttccgctt gagatcatg 
      ttgtcgttg ttaaatcaa ctggcggag attgaatat 
      cgtctagtc caaggggcc aacgaaaac atacaactc 
      actgctctg atcgccgcc tttcacaag gccaaagaa 
      gcgtctcct cccgaaaca gtgtaa
    • >TC034140 RB
      atggttaaa agcgagaca cgcgtcaat ttgccttgg 
      gtcgaaaaa taccgcccc agcagtctg gacgacttg 
      gtgtctcac gacgatatt atcaagaca atcggtaaa 
      tttattagg gaggaccag gtcccccac ttgttgttc 
      tatggcccc cctggaacg ggcaaaact agcactatt 
      ctggcttgc gcccgccag ctttacacc ccggcccag 
      ttttcctca atggttttg gaattgaat gcctctgat 
      gatcggggt attggtatt gtaagggga caaatcctc 
      acgtttgcg agcacacgc acaattttc aagtcaggc 
      ttcaagctg attattttg gacgaagcg gacgcaatg 
      accatggac gcccaaaac gccctcagg agaagtaag 
      tag
    • >TC034140 RC
      atggttaaa agcgagaca cgcgtcaat ttgccttgg 
      gtcgaaaaa taccgcccc agcagtctg gacgacttg 
      gtgtctcac gacgatatt atcaagaca atcggtaaa 
      tttattagg gaggaccag gtcccccac ttgttgttc 
      tatggcccc cctggaacg ggcaaaact agcactatt 
      ctggcttgc gcccgccag ctttacacc ccggcccag 
      ttttcctca atggttttg gaattgaat gcctctgat 
      gatcggggt attggtatt gtaagggga caaatcctc 
      acgtttgcg agcacacgc acaattttc aagtcaggc 
      ttcaagctg attattttg gacgaagcg gacgcaatg 
      accatggac gcccaaaac gccctcagg agaacacct 
      tactattta attgccatt taccagtta taa
    mRNA sequence
    • >TC034140 RA
      acgattggc aattctgtg ccacattcc gccagattt 
      tggcgcctt tactttgat agtttttca ttttcctgt 
      tagttttcc gagtgttag tgagcactg taccttcaa 
      aattaacac tcggtgtac caaaaaagc gaaaattat 
      cacccacat ttgcctgga cttgcccta actttcaat 
      tcgccaaac ttgaaaaag cgaggttaa gtgttactg 
      tttgtgtta catttttca aaattatac gatggttaa 
      aagcgagac acgcgtcaa tttgccttg ggtcgaaaa 
      ataccgccc cagcagtct ggacgactt ggtgtctca 
      cgacgatat tatcaagac aatcggtaa atttattag 
      ggaggacca ggtccccca cttgttgtt ctatggccc 
      ccctggaac gggcaaaac tagcactat tctggcttg 
      cgcccgcca gctttacac cccggccca gttttcctc 
      aatggtttt ggaattgaa tgcctctga tgatcgggg 
      tattggtat tgtaagggg acaaatcct cacgtttgc 
      gagcacacg cacaatttt caagtcagg cttcaagct 
      gattatttt ggacgaagc ggacgcaat gaccatgga 
      cgcccaaaa cgccctcag gagaatcat cgagaagta 
      cacggaaaa cgtgcgctt ttgcataat ttgcaatta 
      cttgagtaa gattattcc ggccctaca gtcacgctg 
      tacgaggtt taggttcgc cccactggc ccccgaaca 
      gattctgcc acgtttgaa ttatgtcat cgatgagga 
      gaaggttaa ggtgacaga cgatggcaa acaagcgct 
      tctcacttt ggcaaacgg cgacatgcg gaaagtgtt 
      aaatgtgct ccagagcac gtggttggc ttatcaaaa 
      cgtgacgga ggataacgt ctacacgtg cgtgggaca 
      ccctttgaa gaacgatat tgaaagtat tgtcaaatg 
      gctacttaa ccaggactt ccaaactgc gtttaaaaa 
      tattaagga actgaaggg ggttaaagg cttggcttt 
      gaatgatat cattacgga agtccaccg ttacgttca 
      tagaattga atttccgct tgagatcat gttgtcgtt 
      gttaaatca actggcgga gattgaata tcgtctagt 
      ccaaggggc caacgaaaa catacaact cactgctct 
      gatcgccgc ctttcacaa ggccaaaga agcgtctcc 
      tcccgaaac agtgtaatt ctcccgttc aataaaaca 
      ctagctaga ta
    • >TC034140 RB
      acgattggc aattctgtg ccacattcc gccagattt 
      tggcgcctt tactttgat agtttttca ttttcctgt 
      tagttttcc gagtgttag tgagcactg taccttcaa 
      aattaacac tcggtgtac caaaaaagc gaaaattat 
      cacccacat ttgcctgga cttgcccta actttcaat 
      tcgccaaac ttgaaaaag cgaggttaa gtgttactg 
      tttgtgtta catttttca aaattatac gatggttaa 
      aagcgagac acgcgtcaa tttgccttg ggtcgaaaa 
      ataccgccc cagcagtct ggacgactt ggtgtctca 
      cgacgatat tatcaagac aatcggtaa atttattag 
      ggaggacca ggtccccca cttgttgtt ctatggccc 
      ccctggaac gggcaaaac tagcactat tctggcttg 
      cgcccgcca gctttacac cccggccca gttttcctc 
      aatggtttt ggaattgaa tgcctctga tgatcgggg 
      tattggtat tgtaagggg acaaatcct cacgtttgc 
      gagcacacg cacaatttt caagtcagg cttcaagct 
      gattatttt ggacgaagc ggacgcaat gaccatgga 
      cgcccaaaa cgccctcag gagaagtaa gtagggtta 
      ggttgttaa tgtttgtat ggaagttgc taatagcac 
      cttactatt taattgcc
    • >TC034140 RC
      acgattggc aattctgtg ccacattcc gccagattt 
      tggcgcctt tactttgat agtttttca ttttcctgt 
      tagttttcc gagtgttag tgagcactg taccttcaa 
      aattaacac tcggtgtac caaaaaagc gaaaattat 
      cacccacat ttgcctgga cttgcccta actttcaat 
      tcgccaaac ttgaaaaag cgaggttaa gtgttactg 
      tttgtgtta catttttca aaattatac gatggttaa 
      aagcgagac acgcgtcaa tttgccttg ggtcgaaaa 
      ataccgccc cagcagtct ggacgactt ggtgtctca 
      cgacgatat tatcaagac aatcggtaa atttattag 
      ggaggacca ggtccccca cttgttgtt ctatggccc 
      ccctggaac gggcaaaac tagcactat tctggcttg 
      cgcccgcca gctttacac cccggccca gttttcctc 
      aatggtttt ggaattgaa tgcctctga tgatcgggg 
      tattggtat tgtaagggg acaaatcct cacgtttgc 
      gagcacacg cacaatttt caagtcagg cttcaagct 
      gattatttt ggacgaagc ggacgcaat gaccatgga 
      cgcccaaaa cgccctcag gagaacacc ttactattt 
      aattgccat ttaccagtt ataaagttc agtacaagg 
      tgctaatcc atgagtgat atgtgtaca aggg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY