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Gene information
General information
TC006623 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12068966..12070240
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006623
    iBeetle screen iB_09392

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 6 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006623 RA
      MDPGRVKFS YLNPYCTEK CVNNLADGK LQCDCADSG 
      IYNWIWEKQ DDTCALLSK NNREVTFHP VYSSGTAAL 
      RGSKPLEKN RHHFWEMKM LSNLYGTDV MVGVGTSEI 
      TLADWRYRF FSLLGFDKE SWGYSYNGL VQHDNVTRC 
      YGSKFGLGS LIGVHLDMY NGTLEYYFN RKPLGVAFQ 
      GLKSHKLYP MISSTAAQS AMKMTCAIS IESSLQLTC 
      LQCIIKHPE LYKQFKSIP GLAKIYEHN FFWMLPKEY 
      EDPKRKAKE NIWSSKRRK MLIPIKWPR CLDSEQQTL 
      KISLHFTNY SDSESSCDM NYDGSGDAS TSKEV
    CDS sequence
    • >TC006623 RA
      atggatccc ggtagggta aagttcagt tacttgaat 
      ccgtactgt acggaaaag tgcgtcaac aacttggcg 
      gacggtaaa ttgcagtgt gactgtgca gattcggga 
      atttacaac tggatttgg gaaaagcaa gatgacacg 
      tgtgcctta ttgtcgaaa aataaccgg gaggtcaca 
      tttcatccc gtttacagt tcgggaacg gcggccctg 
      agggggagc aagcccctt gaaaagaac aggcaccat 
      ttctgggag atgaaaatg ctgtcgaat ttgtacgga 
      acagatgtt atggtagga gtagggact tcggagata 
      acactagcc gattggcga taccgcttt ttcagtttg 
      ttggggttt gataaagaa tcctggggc tattcgtac 
      aatggattg gtacaacat gacaatgtc actagatgt 
      tacggctca aaattcggc ttaggtagc ttgattggg 
      gtgcacttg gacatgtat aacggcact ctggaatat 
      tatttcaac aggaaacct ttaggtgtt gcattccaa 
      gggttgaaa tcacacaaa ctttacccc atgataagt 
      tcaacagca gcacaatca gcaatgaaa atgacttgt 
      gccatttca attgaatca tctttacaa ctcacttgc 
      cttcagtgt attatcaaa catccagaa ttgtacaaa 
      cagttcaag agcatccct ggattagct aaaatctac 
      gaacacaat tttttctgg atgcttccg aaagaatat 
      gaggaccct aaacgaaag gctaaagaa aacatatgg 
      tcgtccaag cgacgaaaa atgctgata ccgatcaag 
      tggccgcgc tgtctcgac tctgaacaa caaacgctc 
      aagatctcg ttacatttc accaactat agtgactca 
      gaaagtagc tgtgatatg aattatgat ggttccgga 
      gatgcatca acttcaaag gaggtttaa
    mRNA sequence
    • >TC006623 RA
      ctaaaccaa catggcgtt tccaaccta agatgtaaa 
      taaacgtcc agctgcagg gcattaaac caagttgta 
      ttttttcga attgtaagt tcgatggat cccggtagg 
      gtaaagttc agttacttg aatccgtac tgtacggaa 
      aagtgcgtc aacaacttg gcggacggt aaattgcag 
      tgtgactgt gcagattcg ggaatttac aactggatt 
      tgggaaaag caagatgac acgtgtgcc ttattgtcg 
      aaaaataac cgggaggtc acatttcat cccgtttac 
      agttcggga acggcggcc ctgaggggg agcaagccc 
      cttgaaaag aacaggcac catttctgg gagatgaaa 
      atgctgtcg aatttgtac ggaacagat gttatggta 
      ggagtaggg acttcggag ataacacta gccgattgg 
      cgataccgc tttttcagt ttgttgggg tttgataaa 
      gaatcctgg ggctattcg tacaatgga ttggtacaa 
      catgacaat gtcactaga tgttacggc tcaaaattc 
      ggcttaggt agcttgatt ggggtgcac ttggacatg 
      tataacggc actctggaa tattatttc aacaggaaa 
      cctttaggt gttgcattc caagggttg aaatcacac 
      aaactttac cccatgata agttcaaca gcagcacaa 
      tcagcaatg aaaatgact tgtgccatt tcaattgaa 
      tcatcttta caactcact tgccttcag tgtattatc 
      aaacatcca gaattgtac aaacagttc aagagcatc 
      cctggatta gctaaaatc tacgaacac aattttttc 
      tggatgctt ccgaaagaa tatgaggac cctaaacga 
      aaggctaaa gaaaacata tggtcgtcc aagcgacga 
      aaaatgctg ataccgatc aagtggccg cgctgtctc 
      gactctgaa caacaaacg ctcaagatc tcgttacat 
      ttcaccaac tatagtgac tcagaaagt agctgtgat 
      atgaattat gatggttcc ggagatgca tcaacttca 
      aaggaggtt taataaaga tttattcat attgagtct 
      ttcattgac caatccgag t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY