Status
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Gene information
General information
TC005511 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11893082..11894339
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005511
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09391

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005511 RA
      MEILELYSG IGGMHWALK VSGVEGTIK AAVDINPTA 
      NSVYKHNFP HINLLNRNV QSLTPQFIN KLGVNTILM 
      SPPCQPFTR NGLQEDIND ERTKSFIHV LAILPDLKV 
      TRILIENVK GFERSKMRD LLIETLEKC GFNYQEFIL 
      TPTQIGIPN TRHRYYCLA KKPPNVFNF KTGVLKTEF 
      PNQQNAPHC FEISKVLEQ NELTPYYLT DKVLTNYLE 
      TTDIRYSTS RNTCCFTKA YGRYVKGTG SVYSDLPEI 
      TPEIFNQLS DHEPGSSAY LKLAHGLKM RFFTPREVG 
      RLMSFPEDF TFPENTSDK QKYMLLGNS INVRVVAEL 
      IKLLQ
    CDS sequence
    • >TC005511 RA
      atggaaatt ttggaactg tacagtggc attgggggg 
      atgcattgg gcgttaaaa gttagtggg gtagaaggc 
      acaattaaa gcagcagtc gacatcaac cccacagcc 
      aattcagtc tataaacac aatttcccg cacataaat 
      ctactcaat cgcaacgtt caaagcctg acaccacag 
      ttcataaat aaattaggc gttaacaca attctaatg 
      tcgccccct tgtcagccc tttacccga aacggcctt 
      caggaggac atcaacgat gaacgaacc aaatctttc 
      atccacgtt ttagcgatt ttacctgat ctcaaagtg 
      actagaatc ttaatagaa aacgtgaaa ggttttgaa 
      cggtcaaaa atgagggac ttgttaatt gaaacgctg 
      gaaaaatgc ggctttaat taccaggaa tttattctc 
      acgcctact caaattggg atacctaac accaggcat 
      cggtactat tgtctcgcc aaaaaacct cccaatgtg 
      ttcaatttt aagacagga gttttgaaa acggaattt 
      cctaatcaa caaaacgcc cctcattgc tttgaaatc 
      agcaaagtt ttggaacaa aacgaatta actccgtat 
      tatttaacc gataaagtt ctaacaaat tatttggaa 
      accacagat atacgttat agcacttcg agaaacacg 
      tgttgtttc actaaggct tacggacgt tacgttaaa 
      ggcaccgga agtgtttat agcgatttg cccgaaatt 
      acacctgaa atattcaac caactttcc gaccacgag 
      cctggaagt agcgcctat ttgaaatta gcacatggg 
      ttgaaaatg cggtttttt acacctagg gaggttggc 
      aggctaatg agttttcct gaagatttt acattcccg 
      gaaaataca agcgataaa caaaagtat atgttactg 
      gggaacagt attaacgtc cgtgttgta gcagaatta 
      ataaaacta ttgcaatga
    mRNA sequence
    • >TC005511 RA
      aatgaggtt aacttaacc tataataaa aaacaaagc 
      aaaaaatgg aaattttgg aactgtaca gtggcattg 
      gggggatgc attgggcgt taaaagtta gtggggtag 
      aaggcacaa ttaaagcag cagtcgaca tcaacccca 
      cagccaatt cagtctata aacacaatt tcccgcaca 
      taaatctac tcaatcgca acgttcaaa gcctgacac 
      cacagttca taaataaat taggcgtta acacaattc 
      taatgtcgc ccccttgtc agcccttta cccgaaacg 
      gccttcagg aggacatca acgatgaac gaaccaaat 
      ctttcatcc acgttttag cgattttac ctgatctca 
      aagtgacta gaatcttaa tagaaaacg tgaaaggtt 
      ttgaacggt caaaaatga gggacttgt taattgaaa 
      cgctggaaa aatgcggct ttaattacc aggaattta 
      ttctcacgc ctactcaaa ttgggatac ctaacacca 
      ggcatcggt actattgtc tcgccaaaa aacctccca 
      atgtgttca attttaaga caggagttt tgaaaacgg 
      aatttccta atcaacaaa acgcccctc attgctttg 
      aaatcagca aagttttgg aacaaaacg aattaactc 
      cgtattatt taaccgata aagttctaa caaattatt 
      tggaaacca cagatatac gttatagca cttcgagaa 
      acacgtgtt gtttcacta aggcttacg gacgttacg 
      ttaaaggca ccggaagtg tttatagcg atttgcccg 
      aaattacac ctgaaatat tcaaccaac tttccgacc 
      acgagcctg gaagtagcg cctatttga aattagcac 
      atgggttga aaatgcggt tttttacac ctagggagg 
      ttggcaggc taatgagtt ttcctgaag attttacat 
      tcccggaaa atacaagcg ataaacaaa agtatatgt 
      tactgggga acagtatta acgtccgtg ttgtagcag 
      aattaataa aactattgc aatgattca gtcgtttta 
      ttgggtgat tccccgttg tgaatgcga cagttcgta 
      ctaatgaca attataacg cccgcgaaa cgacaaaat 
      ttgtcacaa aaatatttt atttaacaa taataatcc 
      cacagtaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY