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Gene information
General information
TC006604 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11856805..11860680
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006604
    iBeetle screen iB_09390

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4

    vitellogenic egg chamber colour darker - Number of animals showing the phenotype: 2

    tropharium structure irregular -
    tropharium structure irregular - Number of animals showing the phenotype: 4

    egg chamber structure degenerated - Number of animals showing the phenotype: 4

    previtellogenic egg chamber potentiallynumber decreased - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006604 RA
      MTSLFDQFE QASTKMRNN NFTSAEMSS LGYINMTLD 
      QDAPIFSKT KKDFTPPDK ITHVAISNK QLAVALGNN 
      TLFRMNLHN PQQQDEISL SKYTSTCRL TNLFMDPTG 
      NHLLLTFAP KSLEGGPEL LYLARKSNK LKTTTKFRG 
      HEFTDVAWN HLNESESTT GPILLGTSK GLIFETEIV 
      LEGDKFFTS GFSSSFEQY WRQVFDIGK GSNTPITGL 
      DYHKVLGTD KYIIFAATP TRLYYFTGR AEHEEKPLL 
      QQVFNRYLN IPEKETYLE RDSSLKYSR LKFWSENLT 
      VPNAFAWMT EKGVTYCQF ESGFDDSMA TLKAKTRLI 
      EYPKPLYED YSALEKFPI ALALTEFHV LLAYTDAIK 
      GVCLLNEEV VYEDNYNEA FGKLVNIVK DGSTGEIWA 
      VTENAVFRF KVTKEERNV WQIFCENQQ FDLAKKYSR 
      GNEAFYNQV LIKEADMLF NNKQYELSA QRYAETQSS 
      FEEICLKFI QVDQQDSLK IFLRRKLDT LKPQDKTQI 
      TMIVLWVVE LYLSKLEEK RLAGLEQSA AYLDIQKEF 
      EVFLALTEV SDCIKKNKS TIYELMASH GDKSNLIKL 
      TIVNKDFEQ LIRHHIYKN SFHEALEVL KSQNKYELY 
      YQFAPILMQ EVPKYTVKA LIEQGKRLL PVRLLPALV 
      TCEGEFHAK EVMKYLEFC TDKLKNTDR AIHNFLLSL 
      YAKHDKAKL MQYFTSQGQ ELSLVNYDV HFALRLCQD 
      DPDLKEACV HLSGLLGLW ESAVELALT DNNLKLAKQ 
      LADMPPEED VELRKKLWL KIAQHVVSG KDDIQQAME 
      FLKQCDLIR IEDILPFFS DFVTIDHFK EAICSSLKE 
      YNQHIQDLK DEMEEATKS AQLVREEIQ SFRNRYTFI 
      SSEENCEIC NGKLMVRPF YMFPCQHKF HTDCLMNEL 
      NPLLGPAKK NKLADLERQ LKILNTQAN VDNVSTGSG 
      MSAREIVKS EIDNIVASE CLFCGENMI RNIDKPFIE 
      DYDYERIAK EWE
    CDS sequence
    • >TC006604 RA
      atgacctcg ttattcgat cagttcgaa caagcgtcc 
      acgaaaatg cgcaacaat aacttcacc tcggccgaa 
      atgagctca ttggggtac attaacatg acactggac 
      caagacgcc cccattttc agtaagacc aagaaggat 
      tttacgccg cctgataaa atcacacat gttgccatc 
      tccaataaa caactagca gtggctcta gggaataac 
      actttgttc agaatgaac ttacacaat ccccaacaa 
      caagacgaa atttcccta tcgaaatac acctcaact 
      tgccgtttg acaaactta ttcatggac ccgactggc 
      aaccacttg ttgctaact ttcgccccc aaaagcctg 
      gaagggggc cctgaactg ctctacttg gcgcgcaaa 
      tcgaacaaa ttgaagacc acgacgaaa ttccgcgga 
      cacgaattt accgacgtt gcgtggaat cacttaaat 
      gaatccgaa agtaccact gggcccata ttgttaggg 
      acttccaaa ggcttgatc ttcgaaacg gaaatcgtg 
      cttgaaggc gataagttc ttcacgtcc ggattttca 
      tcaagcttt gaacagtat tggagacag gttttcgac 
      attggcaaa ggttcaaac acgccaatc acaggcctg 
      gactaccac aaggtgctg ggtaccgac aagtacatc 
      atttttgcg gccacgccc acccgcctg tactacttt 
      acgggacgg gccgaacac gaggaaaaa ccgcttctt 
      cagcaggtt ttcaaccga tatttgaat atcccggaa 
      aaggagacg tatctagag cgcgacagc agtttaaaa 
      tactctcga ttgaaattc tggtcggaa aatttaaca 
      gttccgaac gcctttgcg tggatgacc gaaaagggc 
      gtgacttac tgccagttc gagtcggga ttcgacgac 
      agtatggcc acactcaag gcaaaaacg cgcctgatc 
      gaatacccc aaacccttg tacgaggat tattcggcc 
      cttgaaaag ttcccaata gctttggct ttgacggaa 
      tttcacgtc ttgttggct tacactgac gcaatcaaa 
      ggcgtgtgt ctcttgaac gaggaggtt gtgtatgag 
      gataattac aatgaggcg tttggcaaa ctggttaat 
      attgttaag gatggcagc actggcgag atttgggcg 
      gtgactgaa aacgcggtt tttcgcttc aaagtcaca 
      aaagaggag cgaaacgtt tggcaaatt ttctgcgag 
      aaccaacag tttgacctc gccaaaaaa tactcgaga 
      gggaacgaa gcgttctac aaccaggtc ttaatcaaa 
      gaggctgat atgttgttc aataataaa caatacgag 
      ctcagtgcg caacgttac gccgaaaca cagtccagt 
      tttgaggag atttgtttg aagtttatc caagtcgat 
      caacaagac tcgttaaag attttcctg aggcggaaa 
      ttagacacg ctcaagccg caagacaag actcaaatc 
      accatgatc gtcctttgg gtggtcgaa ttatatttg 
      agcaagttg gaggagaag agattggcg ggtttggag 
      caaagtgcg gcttattta gacatccag aaggagttt 
      gaggtgttt ttggccctg acggaggtt tcggattgc 
      atcaagaag aacaaatct acgatttac gaactgatg 
      gccagtcac ggcgataag agcaatttg attaaattg 
      acgattgtt aacaaggat tttgagcaa ttgattcgt 
      caccacatc tacaagaac agcttccat gaggctctt 
      gaagtcctc aaaagccaa aacaagtac gaactttac 
      taccaattc gccccaatt ctcatgcaa gaagtgccg 
      aaatacaca gtcaaggct ttaatcgaa caggggaaa 
      agactgctc cctgttcgc ctcctcccg gctttagtc 
      acctgcgaa ggcgagttt cacgccaag gaagtgatg 
      aaatatctc gaattctgc accgacaag ctcaaaaac 
      accgacaga gccattcac aatttccta ctttcgttg 
      tacgccaaa cacgataag gccaaactg atgcaatat 
      ttcacctca caagggcag gaattaagt ttggttaat 
      tacgacgta cactttgcc ttaagattg tgtcaggat 
      gatcctgat ttgaaggag gcttgcgta catttatca 
      ggactgtta ggcttgtgg gaatcggca gttgagttg 
      gctttgact gataataat ttgaaattg gccaaacaa 
      ttggcggac atgccgcca gaagaggac gtcgaattg 
      cggaaaaaa ctgtggttg aaaatagcc caacatgtg 
      gtgagtggc aaggacgac atacagcaa gcgatggaa 
      ttcctcaaa cagtgcgac ttgatccga atcgaagac 
      attctcccg ttcttctcc gattttgtc acaattgac 
      cacttcaaa gaggcgatt tgtagctcg ttgaaagag 
      tacaaccaa cacatccaa gacttgaaa gacgaaatg 
      gaggaagca acaaaatcg gcacaacta gtccgcgag 
      gaaattcaa tcgttccgc aaccgctac acttttata 
      agtagcgaa gaaaattgc gagatttgt aatggaaag 
      ttgatggtt aggccgttt tacatgttt ccctgccag 
      cacaagttc cacactgat tgtttaatg aacgaattg 
      aacccgctt ttaggtccg gccaaaaag aacaaattg 
      gcggatttg gaacgccaa ttgaagatt ttaaatacg 
      caagcgaac gtcgataat gtgagcaca ggttctgga 
      atgtcagcg agggaaata gtcaagtcg gaaattgat 
      aatattgtt gcgtccgag tgtttgttt tgcggcgaa 
      aatatgatt aggaatatc gataaaccg tttatagag 
      gattacgat tacgaacgg atcgcaaag gagtgggaa 
      tag
    mRNA sequence
    • >TC006604 RA
      gaaaaataa actgtggaa tgataatca caatcagtc 
      gttgcactt atgatatta tgattattt cgtattttg 
      tgtaaatag gaagtgcga cactgacct aacctcact 
      tattatcct attaatcaa acaaaaaag cagcaaaat 
      gacctcgtt attcgatca gttcgaaca agcgtccac 
      gaaaatgcg caacaataa cttcacctc ggccgaaat 
      gagctcatt ggggtacat taacatgac actggacca 
      agacgcccc cattttcag taagaccaa gaaggattt 
      tacgccgcc tgataaaat cacacatgt tgccatctc 
      caataaaca actagcagt ggctctagg gaataacac 
      tttgttcag aatgaactt acacaatcc ccaacaaca 
      agacgaaat ttccctatc gaaatacac ctcaacttg 
      ccgtttgac aaacttatt catggaccc gactggcaa 
      ccacttgtt gctaacttt cgcccccaa aagcctgga 
      agggggccc tgaactgct ctacttggc gcgcaaatc 
      gaacaaatt gaagaccac gacgaaatt ccgcggaca 
      cgaatttac cgacgttgc gtggaatca cttaaatga 
      atccgaaag taccactgg gcccatatt gttagggac 
      ttccaaagg cttgatctt cgaaacgga aatcgtgct 
      tgaaggcga taagttctt cacgtccgg attttcatc 
      aagctttga acagtattg gagacaggt tttcgacat 
      tggcaaagg ttcaaacac gccaatcac aggcctgga 
      ctaccacaa ggtgctggg taccgacaa gtacatcat 
      ttttgcggc cacgcccac ccgcctgta ctactttac 
      gggacgggc cgaacacga ggaaaaacc gcttcttca 
      gcaggtttt caaccgata tttgaatat cccggaaaa 
      ggagacgta tctagagcg cgacagcag tttaaaata 
      ctctcgatt gaaattctg gtcggaaaa tttaacagt 
      tccgaacgc ctttgcgtg gatgaccga aaagggcgt 
      gacttactg ccagttcga gtcgggatt cgacgacag 
      tatggccac actcaaggc aaaaacgcg cctgatcga 
      ataccccaa acccttgta cgaggatta ttcggccct 
      tgaaaagtt cccaatagc tttggcttt gacggaatt 
      tcacgtctt gttggctta cactgacgc aatcaaagg 
      cgtgtgtct cttgaacga ggaggttgt gtatgagga 
      taattacaa tgaggcgtt tggcaaact ggttaatat 
      tgttaagga tggcagcac tggcgagat ttgggcggt 
      gactgaaaa cgcggtttt tcgcttcaa agtcacaaa 
      agaggagcg aaacgtttg gcaaatttt ctgcgagaa 
      ccaacagtt tgacctcgc caaaaaata ctcgagagg 
      gaacgaagc gttctacaa ccaggtctt aatcaaaga 
      ggctgatat gttgttcaa taataaaca atacgagct 
      cagtgcgca acgttacgc cgaaacaca gtccagttt 
      tgaggagat ttgtttgaa gtttatcca agtcgatca 
      acaagactc gttaaagat tttcctgag gcggaaatt 
      agacacgct caagccgca agacaagac tcaaatcac 
      catgatcgt cctttgggt ggtcgaatt atatttgag 
      caagttgga ggagaagag attggcggg tttggagca 
      aagtgcggc ttatttaga catccagaa ggagtttga 
      ggtgttttt ggccctgac ggaggtttc ggattgcat 
      caagaagaa caaatctac gatttacga actgatggc 
      cagtcacgg cgataagag caatttgat taaattgac 
      gattgttaa caaggattt tgagcaatt gattcgtca 
      ccacatcta caagaacag cttccatga ggctcttga 
      agtcctcaa aagccaaaa caagtacga actttacta 
      ccaattcgc cccaattct catgcaaga agtgccgaa 
      atacacagt caaggcttt aatcgaaca ggggaaaag 
      actgctccc tgttcgcct cctcccggc tttagtcac 
      ctgcgaagg cgagtttca cgccaagga agtgatgaa 
      atatctcga attctgcac cgacaagct caaaaacac 
      cgacagagc cattcacaa tttcctact ttcgttgta 
      cgccaaaca cgataaggc caaactgat gcaatattt 
      cacctcaca agggcagga attaagttt ggttaatta 
      cgacgtaca ctttgcctt aagattgtg tcaggatga 
      tcctgattt gaaggaggc ttgcgtaca tttatcagg 
      actgttagg cttgtggga atcggcagt tgagttggc 
      tttgactga taataattt gaaattggc caaacaatt 
      ggcggacat gccgccaga agaggacgt cgaattgcg 
      gaaaaaact gtggttgaa aatagccca acatgtggt 
      gagtggcaa ggacgacat acagcaagc gatggaatt 
      cctcaaaca gtgcgactt gatccgaat cgaagacat 
      tctcccgtt cttctccga ttttgtcac aattgacca 
      cttcaaaga ggcgatttg tagctcgtt gaaagagta 
      caaccaaca catccaaga cttgaaaga cgaaatgga 
      ggaagcaac aaaatcggc acaactagt ccgcgagga 
      aattcaatc gttccgcaa ccgctacac ttttataag 
      tagcgaaga aaattgcga gatttgtaa tggaaagtt 
      gatggttag gccgtttta catgtttcc ctgccagca 
      caagttcca cactgattg tttaatgaa cgaattgaa 
      cccgctttt aggtccggc caaaaagaa caaattggc 
      ggatttgga acgccaatt gaagatttt aaatacgca 
      agcgaacgt cgataatgt gagcacagg ttctggaat 
      gtcagcgag ggaaatagt caagtcgga aattgataa 
      tattgttgc gtccgagtg tttgttttg cggcgaaaa 
      tatgattag gaatatcga taaaccgtt tatagagga 
      ttacgatta cgaacggat cgcaaagga gtgggaata 
      ggctgttta aaaaacatt attcgtaac ccaatttga 
      tctctattt tattgtatt gtattgctc tactgtgga 
      ggcattaaa ccatgtatg tgattttcc gtatctggg 
      agcgataat aaactgttg cttgtttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY