Status
Please wait ...
Gene information
General information
TC005551 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11405923..11407607
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005551
    iBeetle screen iB_09385

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005551 RA
      MKKTGNKWL GLSLDLENG AQNGHPPKP AIPSKNGTS 
      DLSPVPKKR SFNFHQRLA PKTVPDLAV HSKKIAEVE 
      HWLQNNVCD NKNNSPNAK FLLISGPPG SGKSITIQV 
      LCKKLSIDI TEWVNPLDQ DFEVCRGAN QVTRFVEFL 
      TESKWNSLF STGNKKVIL VEEFPNTFL RNCDEFGPV 
      LEECYYKGN YPVIFVCTD TFDNKINLS EKLFSEDVR 
      VKYKIATIS FNGCAPTLM KSALKRAQN LVQGNPDLF 
      KIPSQSTVT AIISTAMGD LKCALNQYY FAALLGTSD 
      LPTIQTQKS GTKRKRDSS TSIQIMSRD ENLGLFHAL 
      GRVLNPKLG ENGRLACDF DRLVNEFTT QPGLFTGFL 
      FENYLKYFG DLVDASQAS ENLSLGQTF LEKLVDRHD 
      ILDYAIWVS VLGLMVHNG HKVSRWNQI KGPTKVQKV 
      VPNREVPVT DLYYYNVIN NKTNSGFVL S
    CDS sequence
    • >TC005551 RA
      atgaaaaaa acgggaaat aaatggtta ggtttgtcg 
      cttgacttg gaaaacggg gcccagaat ggccatccc 
      cccaaacct gcaatccct tcgaaaaac ggcacttcg 
      gacctgtcc ccagtgcct aaaaaacgc agctttaac 
      tttcaccaa cgcctggcg ccgaaaacg gtgcccgac 
      ttggccgtt cattcgaaa aaaatcgcc gaagtcgag 
      cactggtta caaaacaac gtctgtgat aataaaaac 
      aattctccg aatgcgaaa ttcttgttg atttcgggg 
      cccccaggg tcgggcaaa tcaatcacc attcaggtt 
      ttgtgcaaa aaattatca attgatata actgaatgg 
      gtgaacccc ctagaccag gattttgag gtttgtcgg 
      ggggcgaat caagtgacc cggttcgtt gagtttttg 
      accgaatcc aaatggaat tctttattt tccacaggc 
      aacaaaaaa gtgatttta gttgaggag tttccaaac 
      acgttttta cggaattgt gacgagttt gggccagtt 
      ttagaggag tgttactac aaggggaat tacccggtt 
      attttcgtg tgtacggac actttcgat aataaaatt 
      aacctaagc gagaaatta tttagtgag gacgtaagg 
      gttaagtat aaaattgcg acgattagt ttcaacggt 
      tgcgcccca actttaatg aagagtgcc ctgaaaagg 
      gcccaaaac ctcgtgcag gggaatccc gatttattt 
      aaaattccg tctcaaagt acagtcact gccatcatt 
      tctacagca atgggcgac ttgaaatgc gccctaaat 
      cagtattat tttgccgcc cttttagga acttcagac 
      ctgccaacg attcaaacc caaaaatca ggcaccaaa 
      cgcaaacgt gattcgtcc acttcgatt caaataatg 
      tcacgtgac gaaaatttg ggccttttt cacgccctt 
      ggccgcgtt ttgaacccg aagttgggc gaaaatggc 
      cgacttgcg tgcgatttt gaccgatta gtcaacgaa 
      ttcacaaca cagcctggg cttttcaca gggtttttg 
      ttcgaaaat tacttgaaa tatttcggg gatttggtg 
      gatgccagt caagcgagt gagaatttg agtttaggt 
      caaacattt ctagaaaaa ttggtcgat cgacatgat 
      attctcgac tacgcgatt tgggtctcc gtcttgggc 
      ttgatggta cacaatggg cataaggtg tcgcgatgg 
      aaccaaatc aagggcccc actaaggtg caaaaggtt 
      gtgccaaat cgcgaggtt ccagtgact gatctttat 
      tactacaat gtgattaac aataagact aattcgggc 
      tttgtattg tcgtga
    mRNA sequence
    • >TC005551 RA
      caacaagtt tcaaacaat ctcgaataa aatcatgaa 
      aaaaacggg aaataaatg gttaggttt gtcgcttga 
      cttggaaaa cggggccca gaatggcca tccccccaa 
      acctgcaat cccttcgaa aaacggcac ttcggacct 
      gtccccagt gcctaaaaa acgcagctt taactttca 
      ccaacgcct ggcgccgaa aacggtgcc cgacttggc 
      cgttcattc gaaaaaaat cgccgaagt cgagcactg 
      gttacaaaa caacgtctg tgataataa aaacaattc 
      tccgaatgc gaaattctt gttgatttc ggggccccc 
      agggtcggg caaatcaat caccattca ggttttgtg 
      caaaaaatt atcaattga tataactga atgggtgaa 
      ccccctaga ccaggattt tgaggtttg tcggggggc 
      gaatcaagt gacccggtt cgttgagtt tttgaccga 
      atccaaatg gaattcttt attttccac aggcaacaa 
      aaaagtgat tttagttga ggagtttcc aaacacgtt 
      tttacggaa ttgtgacga gtttgggcc agttttaga 
      ggagtgtta ctacaaggg gaattaccc ggttatttt 
      cgtgtgtac ggacacttt cgataataa aattaacct 
      aagcgagaa attatttag tgaggacgt aagggttaa 
      gtataaaat tgcgacgat tagtttcaa cggttgcgc 
      cccaacttt aatgaagag tgccctgaa aagggccca 
      aaacctcgt gcaggggaa tcccgattt atttaaaat 
      tccgtctca aagtacagt cactgccat catttctac 
      agcaatggg cgacttgaa atgcgccct aaatcagta 
      ttattttgc cgccctttt aggaacttc agacctgcc 
      aacgattca aacccaaaa atcaggcac caaacgcaa 
      acgtgattc gtccacttc gattcaaat aatgtcacg 
      tgacgaaaa tttgggcct ttttcacgc ccttggccg 
      cgttttgaa cccgaagtt gggcgaaaa tggccgact 
      tgcgtgcga ttttgaccg attagtcaa cgaattcac 
      aacacagcc tgggctttt cacagggtt tttgttcga 
      aaattactt gaaatattt cggggattt ggtggatgc 
      cagtcaagc gagtgagaa tttgagttt aggtcaaac 
      atttctaga aaaattggt cgatcgaca tgatattct 
      cgactacgc gatttgggt ctccgtctt gggcttgat 
      ggtacacaa tgggcataa ggtgtcgcg atggaacca 
      aatcaaggg ccccactaa ggtgcaaaa ggttgtgcc 
      aaatcgcga ggttccagt gactgatct ttattacta 
      caatgtgat taacaataa gactaattc gggctttgt 
      attgtcgtg attaataaa tttgaattt ggcgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY