Status
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Gene information
General information
TC006548 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11309640..11317774
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006548
    iBeetle screen iB_09382

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006548 RA
      MTELGVNGN DEKDSDKEA VSDKDLPLS FMQDRHLEP 
      IEGVECITQ TWRMKERMK TVSVALVLC LNVGVDPPD 
      VMKTQPCAR LECWIDPLT LSAPKSIQK IGENLQAQY 
      ERWQPRARY KQSLDPTSE EVKKLCTSL RRNAKEERV 
      LFHYNGHGV PKPTENGEI WVFNRTYTQ YIPLSIYDL 
      QTWMGAPSI YVYDCSNAG IIIESFERF AAQHEAEYE 
      QATSQSRQN PVVAPLPSY KSCIQLAAC GAKEVLPMN 
      PDLPADLFT SCLTTPIKV ALRWFVLQS TSKLVPKVT 
      LDLIDKIPG QITDRRTML GELNWIFTA ITDTIAWNT 
      LQRDLFQKL FRQDLLVAS LFRNFLLAE RILKSCDCT 
      PVSSPLLPP TSQHPMWNA WDLALDLSL GQLPGIINR 
      NEPFQNLPF FEEQLTAFQ VWLELGCEE RSPPEQLPI 
      VLQVLLSQV HRMRALELL GRFLDLGPW AVNLALSVG 
      IFPYVLKLL QSSAKELRP LLVFIWAKI LAVDNTCQG 
      DLVRDSGHK YFLSVLQDT SLSSEYRTQ SAFVLASIV 
      NNFHDGQEA ALKGSLVSI CLEQLNDPS SQLRQWVAI 
      CLGKLWDHY ENARWTGVR DIAHEKLYK LLNDPCPEV 
      RAAAVYALG TFINSVTER SEHANNIDH AIVMTLVKK 
      VSNDMSHLV RKELVNALQ WVVLAFEHV FITLSMKEI 
      LSHYPLQEV NVTSPGLRR IGSRDRLRT SEDSVDKMK 
      RVSSSSSIN NMGQMKGSL SHTGSLGTL PGLGYGSIY 
      MKIWDALCS LETDPHPEV ESMCKTITK YIANKVKES 
      REFVDNKCA SLPPSPNRG NYLSGESPP TLHPATDIP 
      KYTPRGRKI HPNIINEET DMKIKQRKA LVSTKFIEW 
      SCKRFAQSK RKSGFDVES PQFYERDWR YTRNNNIRR 
      EALDETSRI LSSKLESQL FNTRTSLPP SVLQFHPFD 
      QQIAVAGKD HFGIWDWGT GAKTTVCHN KSAKAGSSR 
      ITSLEWINA HDVAMLMVA SDDGSVKVW KPNTGHSRE 
      PTLISAWQA LNDLKWKYS GTLLAWEQY TQTCIAAGD 
      SRIVRFWDV EKELFAYDI MTGADCAIT CIDSTFSGV 
      SHENYSKAY LDREKDDEG ISIDEDFKW TRYGMVVAG 
      FQDGSIRVF DRRCSPTEA RIAVFMEHS GPILGVHLL 
      GNTFYSGST DGHVRVFDV RNHRVVHTT QAIQNNMSC 
      FALHRGTAT YACGSLNQN VSIYGFNGC LLNTIKFYE 
      GFMGHRTGN VTCLNYHPL KVALATGTS DCSVSVYCL 
      EGKR
    CDS sequence
    • >TC006548 RA
      atgacagag ttgggtgtc aacggcaat gacgagaaa 
      gacagtgac aaggaggct gtctccgac aaggacctg 
      cccctgagc ttcatgcaa gacaggcac ttggagccc 
      atcgagggc gtcgagtgc ataacgcag acatggcgc 
      atgaaagaa cgcatgaaa acagtgagt gtggcccta 
      gttctgtgt ctgaacgtg ggcgtggac ccgcccgac 
      gtgatgaaa acccagcca tgtgcgcgt ctggagtgc 
      tggatagat cccttaacc ttgtccgcc ccaaagtcg 
      atccagaaa atcggggag aacctgcag gcgcagtac 
      gagcggtgg cagccccgc gcgcgctac aagcagtcc 
      ctggacccc acttcggaa gaagtcaaa aaactgtgc 
      acttcgctg cgccgcaac gccaaagag gaacgcgtt 
      ctgttccac tacaacggt catggagtt cctaaaccg 
      acggaaaac ggtgaaatc tgggtgttc aatcgaacc 
      tacacgcaa tacatcccc ctgtccatc tacgacctg 
      caaacgtgg atgggggcg ccctcgatc tacgtctac 
      gactgctcg aacgccggg atcataatc gagtctttc 
      gagcggttt gccgcccaa cacgaggcc gagtacgag 
      caggccacg agtcagtcg cggcaaaat ccagtggtg 
      gcgcctttg ccctcctat aagagttgc atacagttg 
      gcggcgtgc ggggcgaag gaagtgcta ccgatgaat 
      cctgattta ccggccgat ttgttcacg tcttgcctt 
      actacgcca atcaaggtc gccttaaga tggttcgtt 
      ctacagtct acctcaaaa ctagtgccc aaagtcacc 
      ctggatttg atcgataaa ataccgggt caaataacc 
      gaccgccgc acaatgctc ggtgagctt aattggatc 
      tttacagca attacagac accatagcc tggaatacg 
      ttacagaga gatcttttt caaaaactc ttccgtcaa 
      gacctcctc gttgcgtcc cttttccgt aatttcctc 
      ctcgctgaa cgcatccta aaatcgtgc gattgcact 
      cccgtcagt tccccactg ttaccacca acctcccaa 
      caccccatg tggaacgca tgggaccta gccctcgac 
      ttgagcctc ggccaactt cccggaatc atcaaccga 
      aacgaacct ttccaaaac ttgcctttc ttcgaagag 
      caattgacc gcgttccaa gtttggcta gaactcgga 
      tgcgaggag cgcagtcca cccgaacaa ttgccaatt 
      gttttacaa gtcctactc agtcaagtc catcggatg 
      cgagcgctt gaactgttg gggcggttt ctcgatttg 
      ggaccgtgg gctgttaat ttggccttg tctgttggg 
      attttccca tacgttctt aaattatta cagagtagt 
      gctaaggag ttgaggcct ttgttggtt ttcatctgg 
      gctaagatt ctagctgtt gataatacg tgtcaaggt 
      gacttggtg agggacagt ggccataaa tatttcttg 
      tcggtactt caggacact tcactttcg tcggaatat 
      cggacacag tcggctttt gttttggcg tcaattgtt 
      aacaatttc cacgacggg caagaagcg gccttgaaa 
      ggttcccta gttagtatc tgtctggaa caattgaac 
      gaccccagt tcacagttg agacaatgg gtcgctatt 
      tgtttaggg aaattatgg gatcattac gaaaacgcc 
      cgctggacc ggtgttaga gacatcgcc catgaaaaa 
      ctctacaaa cttttgaac gacccatgc ccagaggtt 
      cgggcagcg gccgtttac gccttaggt acgtttata 
      aattcggtg accgagcgt tcggaacac gccaataac 
      atcgaccac gcgatagtt atgacccta gttaagaaa 
      gtcagtaat gacatgagc catctagtc cgtaaagaa 
      ttagtaaat gcgctccaa tgggtggtg ttagctttc 
      gaacacgtg tttataact ttgtcaatg aaggagatt 
      ctaagtcac tatcccttg caagaagtc aacgtgact 
      tcgcccggc ttgcgacga atcggcagc cgggaccgg 
      ttacgaact tccgaagac agtgtcgat aaaatgaaa 
      cgggtgtcc tccagctcc tccataaac aacatgggg 
      caaatgaaa ggctccctc agccacacc ggcagcctt 
      ggcacgtta ccaggcctc ggctacggt tcgatttac 
      atgaaaata tgggacgct ttgtgttcg cttgaaacc 
      gacccgcac cccgaagtg gaatcaatg tgtaagact 
      ataaccaag tacatagca aacaaagtg aaggaatcg 
      cgcgagttc gtcgataac aagtgtgca agtttgccc 
      ccgagcccc aaccggggg aactacctg tcgggcgaa 
      agcccccca actctccac cccgccacc gacatcccc 
      aaatacacc ccaaggggg cgcaaaatc caccccaac 
      atcatcaac gaagaaacc gacatgaaa atcaagcaa 
      cgcaaggct ttagtgagt acgaagttc atcgaatgg 
      tcgtgcaaa cgcttcgcg caatcgaag cgaaagagt 
      ggctttgac gtcgaatcg ccccaattt tacgaacgc 
      gattggagg tacaccagg aataataat ataagacgg 
      gaggctttg gacgaaact tcgcgtatc ctttcgtca 
      aaactcgaa tctcagttg ttcaacact aggacctcg 
      ctcccgccc agtgtcctc cagtttcac ccctttgac 
      cagcaaatc gcagtggct gggaaggat catttcggg 
      atttgggat tgggggact ggggctaag acgacagtg 
      tgtcataat aagtcggca aaggctggg tctagtcgg 
      atcacttcg ttggagtgg atcaatgcc catgatgtg 
      gcgatgttg atggtggct tcggacgat ggtagtgtt 
      aaagtttgg aagccgaat acgggtcac agtcgcgaa 
      ccgactctg ataagtgca tggcaggct ttgaatgat 
      ttgaagtgg aaatattca gggacgctg ttggcgtgg 
      gagcagtac acacagacg tgcattgcg gcaggggat 
      tcgaggatt gtccggttt tgggacgtg gagaaggag 
      ctgtttgcc tatgatatt atgacagga gcggactgc 
      gcgatcact tgcatcgac tcgacattt tcgggcgtt 
      tcgcacgaa aactacagt aaagcgtac ctagaccgc 
      gaaaaagac gacgaagga attagcatc gacgaggat 
      ttcaaatgg actcgttac ggcatggtg gtggccggc 
      ttccaagac ggttcaatc cgagtgttt gaccgccgt 
      tgcagcccc acagaggcc cgaatcgcc gtgtttatg 
      gaacattcc ggcccgatt ttaggcgtc cacttactc 
      ggaaacaca ttttatagt ggaagcacc gacggccac 
      gtccgagtc ttcgacgtg cgcaaccac agggttgtg 
      cacacgacc caagccatc cagaacaac atgtcctgc 
      tttgcgctg catcggggg accgccacc tacgcctgc 
      gggtctctg aaccaaaat gtgagcatt tacgggttc 
      aacgggtgt ctcctgaac acgatcaag ttctacgag 
      ggcttcatg ggccaccgt accggcaac gtcacgtgc 
      ctgaactac catccgctg aaggtggcg ctggccaca 
      ggcacgtcc gactgctcg gtgagcgtc tactgtttg 
      gaaggtaaa aggtga
    mRNA sequence
    • >TC006548 RA
      ttggtagta agatggcag ttttgttgt cgtgccatg 
      cgtgtgtaa attttaggt gttgattcc gttttttga 
      tgtaatcgt tacgactta aataaatcc cacttcaca 
      actcctaaa aaacccgaa aacgcggct gaaaactat 
      gacagagtt gggtgtcaa cggcaatga cgagaaaga 
      cagtgacaa ggaggctgt ctccgacaa ggacctgcc 
      cctgagctt catgcaaga caggcactt ggagcccat 
      cgagggcgt cgagtgcat aacgcagac atggcgcat 
      gaaagaacg catgaaaac agtgagtgt ggccctagt 
      tctgtgtct gaacgtggg cgtggaccc gcccgacgt 
      gatgaaaac ccagccatg tgcgcgtct ggagtgctg 
      gatagatcc cttaacctt gtccgcccc aaagtcgat 
      ccagaaaat cggggagaa cctgcaggc gcagtacga 
      gcggtggca gccccgcgc gcgctacaa gcagtccct 
      ggaccccac ttcggaaga agtcaaaaa actgtgcac 
      ttcgctgcg ccgcaacgc caaagagga acgcgttct 
      gttccacta caacggtca tggagttcc taaaccgac 
      ggaaaacgg tgaaatctg ggtgttcaa tcgaaccta 
      cacgcaata catccccct gtccatcta cgacctgca 
      aacgtggat gggggcgcc ctcgatcta cgtctacga 
      ctgctcgaa cgccgggat cataatcga gtctttcga 
      gcggtttgc cgcccaaca cgaggccga gtacgagca 
      ggccacgag tcagtcgcg gcaaaatcc agtggtggc 
      gcctttgcc ctcctataa gagttgcat acagttggc 
      ggcgtgcgg ggcgaagga agtgctacc gatgaatcc 
      tgatttacc ggccgattt gttcacgtc ttgccttac 
      tacgccaat caaggtcgc cttaagatg gttcgttct 
      acagtctac ctcaaaact agtgcccaa agtcaccct 
      ggatttgat cgataaaat accgggtca aataaccga 
      ccgccgcac aatgctcgg tgagcttaa ttggatctt 
      tacagcaat tacagacac catagcctg gaatacgtt 
      acagagaga tctttttca aaaactctt ccgtcaaga 
      cctcctcgt tgcgtccct tttccgtaa tttcctcct 
      cgctgaacg catcctaaa atcgtgcga ttgcactcc 
      cgtcagttc cccactgtt accaccaac ctcccaaca 
      ccccatgtg gaacgcatg ggacctagc cctcgactt 
      gagcctcgg ccaacttcc cggaatcat caaccgaaa 
      cgaaccttt ccaaaactt gcctttctt cgaagagca 
      attgaccgc gttccaagt ttggctaga actcggatg 
      cgaggagcg cagtccacc cgaacaatt gccaattgt 
      tttacaagt cctactcag tcaagtcca tcggatgcg 
      agcgcttga actgttggg gcggtttct cgatttggg 
      accgtgggc tgttaattt ggccttgtc tgttgggat 
      tttcccata cgttcttaa attattaca gagtagtgc 
      taaggagtt gaggccttt gttggtttt catctgggc 
      taagattct agctgttga taatacgtg tcaaggtga 
      cttggtgag ggacagtgg ccataaata tttcttgtc 
      ggtacttca ggacacttc actttcgtc ggaatatcg 
      gacacagtc ggcttttgt tttggcgtc aattgttaa 
      caatttcca cgacgggca agaagcggc cttgaaagg 
      ttccctagt tagtatctg tctggaaca attgaacga 
      ccccagttc acagttgag acaatgggt cgctatttg 
      tttagggaa attatggga tcattacga aaacgcccg 
      ctggaccgg tgttagaga catcgccca tgaaaaact 
      ctacaaact tttgaacga cccatgccc agaggttcg 
      ggcagcggc cgtttacgc cttaggtac gtttataaa 
      ttcggtgac cgagcgttc ggaacacgc caataacat 
      cgaccacgc gatagttat gaccctagt taagaaagt 
      cagtaatga catgagcca tctagtccg taaagaatt 
      agtaaatgc gctccaatg ggtggtgtt agctttcga 
      acacgtgtt tataacttt gtcaatgaa ggagattct 
      aagtcacta tcccttgca agaagtcaa cgtgacttc 
      gcccggctt gcgacgaat cggcagccg ggaccggtt 
      acgaacttc cgaagacag tgtcgataa aatgaaacg 
      ggtgtcctc cagctcctc cataaacaa catggggca 
      aatgaaagg ctccctcag ccacaccgg cagccttgg 
      cacgttacc aggcctcgg ctacggttc gatttacat 
      gaaaatatg ggacgcttt gtgttcgct tgaaaccga 
      cccgcaccc cgaagtgga atcaatgtg taagactat 
      aaccaagta catagcaaa caaagtgaa ggaatcgcg 
      cgagttcgt cgataacaa gtgtgcaag tttgccccc 
      gagccccaa ccgggggaa ctacctgtc gggcgaaag 
      ccccccaac tctccaccc cgccaccga catccccaa 
      atacacccc aagggggcg caaaatcca ccccaacat 
      catcaacga agaaaccga catgaaaat caagcaacg 
      caaggcttt agtgagtac gaagttcat cgaatggtc 
      gtgcaaacg cttcgcgca atcgaagcg aaagagtgg 
      ctttgacgt cgaatcgcc ccaatttta cgaacgcga 
      ttggaggta caccaggaa taataatat aagacggga 
      ggctttgga cgaaacttc gcgtatcct ttcgtcaaa 
      actcgaatc tcagttgtt caacactag gacctcgct 
      cccgcccag tgtcctcca gtttcaccc ctttgacca 
      gcaaatcgc agtggctgg gaaggatca tttcgggat 
      ttgggattg ggggactgg ggctaagac gacagtgtg 
      tcataataa gtcggcaaa ggctgggtc tagtcggat 
      cacttcgtt ggagtggat caatgccca tgatgtggc 
      gatgttgat ggtggcttc ggacgatgg tagtgttaa 
      agtttggaa gccgaatac gggtcacag tcgcgaacc 
      gactctgat aagtgcatg gcaggcttt gaatgattt 
      gaagtggaa atattcagg gacgctgtt ggcgtggga 
      gcagtacac acagacgtg cattgcggc aggggattc 
      gaggattgt ccggttttg ggacgtgga gaaggagct 
      gtttgccta tgatattat gacaggagc ggactgcgc 
      gatcacttg catcgactc gacattttc gggcgtttc 
      gcacgaaaa ctacagtaa agcgtacct agaccgcga 
      aaaagacga cgaaggaat tagcatcga cgaggattt 
      caaatggac tcgttacgg catggtggt ggccggctt 
      ccaagacgg ttcaatccg agtgtttga ccgccgttg 
      cagccccac agaggcccg aatcgccgt gtttatgga 
      acattccgg cccgatttt aggcgtcca cttactcgg 
      aaacacatt ttatagtgg aagcaccga cggccacgt 
      ccgagtctt cgacgtgcg caaccacag ggttgtgca 
      cacgaccca agccatcca gaacaacat gtcctgctt 
      tgcgctgca tcgggggac cgccaccta cgcctgcgg 
      gtctctgaa ccaaaatgt gagcattta cgggttcaa 
      cgggtgtct cctgaacac gatcaagtt ctacgaggg 
      cttcatggg ccaccgtac cggcaacgt cacgtgcct 
      gaactacca tccgctgaa ggtggcgct ggccacagg 
      cacgtccga ctgctcggt gagcgtcta ctgtttgga 
      aggtaaaag gtgacgcga ttcccagcc ctagagctc 
      ggtctggag cctcttggg ctactacta ttagcggaa 
      attttgctt tgcgcttcg gttttactg taaatattt 
      tacgttggt aacttgaaa tttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY