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Gene information
General information
TC034112
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC006528,TC006529
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 11045140..11050973
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034112
    iBeetle screen iB_09379

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber potentially number decreased - Number of animals showing the phenotype: 3

    mature egg not present - Number of animals showing the phenotype: 4

    yolk structure irregular - Number of animals showing the phenotype: 2

    tropharium structure irregular - Number of animals showing the phenotype: 4

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20
    5
    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034112 RA
      MKVVSRKPQ HTSNTHPWV SASLVVLGL GALALAVFW 
      IRKCRSSGA PKHEYSALK TEGGNEKFR AEQQIRNGV 
      FMACKHYLT NNERYELKT QLGEIGSRT DKHWFTVQD 
      TTLGAERLL TLVPLPSMC PLNPSTTTR DTLLELFRG 
      LQHPYIHPV LDIEFWDAG AALISPLNP SGSLKDLIY 
      ASPWHEDYD KKYNTRGDG LPLRTVQCL GRQILEGLL 
      FLRNRYFPP FYHLHSGNV IIQNGVARI AGLENALLG 
      LLPRAPSAP ETLAFGYLL FEMAAGYEL PKPPSPAHL 
      QLELERVPK VAEALELIF QTSRTPALE ELVCCELFR 
      GVELRELRG ASIIQVRSS PEVLELLEV VRNPVPPSP 
      LRRKDVVVI IEDRRLEDI IEEDNEDSD ANDASDNR
    CDS sequence
    • >TC034112 RA
      atgaaagtg gtgtccagg aagccgcag catacctcc 
      aacacccat ccgtgggtt tcggcctcg ttagtcgtg 
      ctaggttta ggagctttg gcgctagcg gtgttttgg 
      attaggaaa tgcaggtcg tccggggcc cccaagcac 
      gaatacagc gccttgaaa accgaggga gggaatgag 
      aagttccgg gccgagcag cagataagg aatggggtt 
      tttatggct tgtaagcac tatcttacg aacaatgag 
      cggtacgag ctgaagacg cagttgggg gagatcggg 
      tcgaggaca gacaagcat tggtttact gtccaagac 
      accactttg ggggcggag aggttgctc acgttagtg 
      cccttaccg tccatgtgt cccttgaac ccttccaca 
      accacgaga gataccctt ctggagctg ttccgtggc 
      ttacaacac ccctacatt catccagtc ctggacatt 
      gagttttgg gacgcgggg gcggctctt atcagtcct 
      ctgaatccg tccggtagt ttgaaggac ctaatctac 
      gccagcccg tggcacgag gactacgac aagaagtac 
      aacacaagg ggagacgga ttacccctc agaacggtc 
      cagtgcctg ggccgccag atcctcgaa ggccttctc 
      tttctgcgc aaccgctac ttcccgccc ttctaccac 
      ctgcactcc ggcaacgtg ataatccag aacggcgtt 
      gcccgaatc gccggccta gaaaacgcc ctcttaggg 
      cttctccct cgggcgccc agcgccccc gaaaccctg 
      gccttcggt taccttttg ttcgaaatg gcagccggg 
      tacgaactt cccaagcct ccaagtcca gcccacttg 
      cagctcgaa ctggagcga gtccccaaa gtggccgaa 
      gccttggag ttgattttc cagactagc cgaactccc 
      gcattggag gagttggtc tgttgtgag ttgttccgc 
      ggggtggag ttgcgggag ttgcgtggg gcgagcata 
      attcaagtg aggtcgtcg ccggaggtg ctggagttg 
      ctggaggtg gtgcggaat ccggtgccg ccgtcgccg 
      ttgagacgc aaagatgta gtcgtgata atagaagac 
      aggcgacta gaagacatt atagaagaa gacaacgaa 
      gatagtgat gcaaacgac gccagtgat aatcggtag
    mRNA sequence
    • >TC034112 RA
      cctcttgtt tatcgactg attccaatt cacggcaat 
      agtgatacc atgcgtgtt tgcaatttg cgtgctttc 
      aggcgttta tttcagttc cggtgttag attcgccaa 
      gtgagcttt tgtgtaatt cattttcgt cgttaacac 
      aagctttta tcagaatcc tgttgctgg cgacatgta 
      gctttttct cccaaccac aaacctcta tcctcttaa 
      aacatgaaa gatgaaagt ggtgtccag gaagccgca 
      gcatacctc caacaccca tccgtgggt ttcggcctc 
      gttagtcgt gctaggttt aggagcttt ggcgctagc 
      ggtgttttg gattaggaa atgcaggtc gtccggggc 
      ccccaagca cgaatacag cgccttgaa aaccgaggg 
      agggaatga gaagttccg ggccgagca gcagataag 
      gaatggggt ttttatggc ttgtaagca ctatcttac 
      gaacaatga gcggtacga gctgaagac gcagttggg 
      ggagatcgg gtcgaggac agacaagca ttggtttac 
      tgtccaaga caccacttt gggggcgga gaggttgct 
      cacgttagt gcccttacc gtccatgtg tcccttgaa 
      cccttccac aaccacgag agataccct tctggagct 
      gttccgtgg cttacaaca cccctacat tcatccagt 
      cctggacat tgagttttg ggacgcggg ggcggctct 
      tatcagtcc tctgaatcc gtccggtag tttgaagga 
      cctaatcta cgccagccc gtggcacga ggactacga 
      caagaagta caacacaag gggagacgg attacccct 
      cagaacggt ccagtgcct gggccgcca gatcctcga 
      aggccttct ctttctgcg caaccgcta cttcccgcc 
      cttctacca cctgcactc cggcaacgt gataatcca 
      gaacggcgt tgcccgaat cgccggcct agaaaacgc 
      cctcttagg gcttctccc tcgggcgcc cagcgcccc 
      cgaaaccct ggccttcgg ttacctttt gttcgaaat 
      ggcagccgg gtacgaact tcccaagcc tccaagtcc 
      agcccactt gcagctcga actggagcg agtccccaa 
      agtggccga agccttgga gttgatttt ccagactag 
      ccgaactcc cgcattgga ggagttggt ctgttgtga 
      gttgttccg cggggtgga gttgcggga gttgcgtgg 
      ggcgagcat aattcaagt gaggtcgtc gccggaggt 
      gctggagtt gctggaggt ggtgcggaa tccggtgcc 
      gccgtcgcc gttgagacg caaagatgt agtcgtgat 
      aatagaaga caggcgact agaagacat tatagaaga 
      agacaacga agatagtga tgcaaacga cgccagtga 
      taatcggta gtagaatcg ttggttgtg gcatttaaa 
      tgcttagag ggtagttca gtagtacca tttaaatgc 
      tgcctctca attacgcaa tgtgtgctg tgttcaggc 
      tttaattgg gtgttattt cgtggccaa agttttgga 
      ccaacctca tgttacaaa ttactaaat gtattttca 
      tataaatcg atcgaataa tcaaaagtg agcaattcc 
      agcactgtt tcatatttt actatcgat ttacgtaga 
      gtatagcaa taatttcct gtattgtag agtttgtaa 
      gtggtagga atcaaaaca ccagacttc tccctttgt 
      cccctttta ttgtaaacg gaccgtcct ttaggacta 
      gaaggcaat acttaatgg cagaaaaca tatccatta 
      ccgtcctag cgtgactgt caattgttc tgtttatag 
      attaccgtt aattaaagc acggaaaaa gttctttag 
      gagaagcaa atttcaaat caaacactt cagttccgt 
      caaacatgg gcgtaagac tttcgtgaa attgttaat 
      acgacaatc acaccgata atgtcaaat gcaatatat 
      tagtcaacg agtctttct tttatgtgt aggcgtgta 
      atttctact gttatttca ataaaattc actgcagtt 
      a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY