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Gene information
General information
TC034845 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10994683..11002650
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034845
    iBeetle screen iB_09378

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg not present - Number of animals showing the phenotype: 4
    vitellogenic egg chamber latenumber increased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber sometimesstructure irregular - Number of animals showing the phenotype: 3
    yolk deposition irregular - Number of animals showing the phenotype: 4

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20
    2
    embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034845 RA
      MAFLEWRKF HFFDLKKDV DEGKIAELF KESKVTVTC 
      SGNNHIVLG DSNGQIFLC SRSWHVRTF RAYELTVEL 
      AHQLRNSPL LVTIGQDET GINPLIKVW DTSRFDKNG 
      TPFCCRISR AIPGNRPVH ASCLCVHEG AQLMAVGFV 
      DGSLVLYRG DITRDRSSK QKLLRDASS TVTGLAFKT 
      TSTNIFLFL ATDSAVMVY NITHKDKEV KFHLDNIGC 
      AKKCSVLAE SMIESHFMV GRNDAIYCY TADGRGPCY 
      AVDGEKVML EWFRSYLVI ISKTTRPNL AITNDNQSA 
      PSQGDLITV LDIHNKFIV FSATVSSIR SVLNEWGAF 
      YILDNDNRL YHLDEKDLQ SKLSLLFKK NLYDVAIRI 
      AKSQQYDSD GLVNIFRQY GDHLCDKGD YVGAIEQYI 
      KTIGKLEPS YVIRKFLDS QHIEKLTMY LEALHKQGH 
      ATEDHTTLL LNCYTKLNN TVGQSNSLK EFILMKEGD 
      LNYDVDIAI KVCRQGSPA EALMLAKKH EKHDWYIKL 
      QIEDHQKYV DVLDYISNL TFENAEFYM KKYGNILIQ 
      NAPYESTQF LKRLCTNYN SHNSLDNSL VGSFELSQK 
      SDPEDYIHL FLNNSERLV EFLEYLIGE GCILSTPVY 
      NTLLEHYLH VWGNLENVS ERNKYAQKT LKLLQNPDI 
      KYDKSQALV VCHMHSFSE GILYLYEEQ KLYQQILRH 
      HITKNDTSS ILACCRRFG HQEPTLWVQ ALWSCVRDT 
      KNPPIDLLN EILTVIAKE RLLSPQLVV DALGTGSVD 
      ITLGHIRSY LTNELQQEQ KKTKEISDL THNYRKDTE 
      KLKEQLETL KSGTIVIQG SRCAACHHP LELPTIHFL 
      CQHSYHQHC FQSFCEEEN ECPACQPEN KNLLELLKA 
      REYNKDLHE TFHSQLEKA QDGFSVAAE YFGRGVFNK 
      YKVIRDEVR ETAPADKIE KKRVVEPEV RNYGLGAEA 
      RLRQVEKQG QVLIPTSEG RVRVQEHRY SSSLEAKNR 
      RVRVQEHRY SSSLEANIS NYVPKSYER KREYSGDIA 
      GTNPFESDY DESKNPFAS EDDDYDENN PFRDDCDKN 
      SNPFYK
    CDS sequence
    • >TC034845 RA
      atggccttt ttggagtgg cgcaagttt catttcttt 
      gacctgaaa aaagacgtg gacgagggg aaaatagcc 
      gagcttttt aaagaatct aaagtcacg gtaacatgt 
      agcggtaac aaccacatt gttttgggc gattcaaac 
      ggccaaatt ttcctgtgt tctcgctcg tggcatgtc 
      cgcactttc cgggcctat gagctaaca gtagagctc 
      gcccaccag ttgagaaac tcccctctt ttggtcaca 
      attgggcaa gatgagact gggattaat cccttgatt 
      aaagtctgg gacacgagc agatttgac aaaaatggg 
      acgccgttt tgttgccgc atttcgcgg gcaattccc 
      gggaaccgg cccgtgcac gcgtcatgt ctgtgtgtc 
      catgagggg gcgcagtta atggccgtt gggtttgtc 
      gatggttct ttggtcttg tatcggggt gatatcacg 
      cgtgaccgg agctccaaa caaaaactc ttgcgtgac 
      gcaagctct actgtgacc ggtcttgca ttcaaaacg 
      acttcaacc aacattttt ttattcctg gctactgat 
      agcgctgtt atggtttat aatattact cataaagat 
      aaagaagtg aagtttcat ttggataac atcggatgt 
      gcgaaaaaa tgcagcgtt ttggccgaa tcgatgatt 
      gagagtcat ttcatggtc ggccgaaat gatgctatt 
      tattgctac acggccgat ggtcgcggc ccttgctat 
      gccgtagac ggcgaaaaa gtcatgtta gagtggttt 
      cggagctac cttgtcatt atctcaaaa acaacacga 
      ccaaacctc gccataaca aacgacaat caaagtgcc 
      cccagccaa ggcgacctg atcacagtt ttagacatt 
      cacaacaaa tttatcgta ttttcggca actgtttca 
      tcaatccgg tcagtttta aatgaatgg ggggccttc 
      tacatcctc gataacgac aacagactt taccacctg 
      gacgagaaa gacctccaa tcgaaatta tcgctcctt 
      ttcaagaaa aatttatac gacgttgca atccgtatc 
      gcaaagtcg caacagtac gattccgac ggccttgtc 
      aacattttc cggcaatac ggcgaccat ttatgcgac 
      aagggtgac tatgtgggg gcgatcgaa cagtacata 
      aagacaatc gggaaattg gagcccagt tatgtcatt 
      cggaaattt ctggactcg caacacatc gaaaaatta 
      acaatgtat ctggaagcc ttacacaag caaggccac 
      gccaccgaa gaccacaca accctcctc ttgaactgc 
      tacacaaaa ctgaacaac acggtcgga caatcgaac 
      agtttgaag gagtttatt ttgatgaaa gagggcgat 
      ttgaactac gacgtagac attgcgatt aaagtgtgc 
      cggcaaggg agcccagct gaggcccta atgttggcc 
      aaaaagcac gaaaaacac gactggtac atcaaacta 
      caaatcgaa gaccaccaa aaatacgtg gacgtgctg 
      gactacatt tcgaaccta actttcgaa aacgccgaa 
      ttttacatg aaaaaatac ggcaatatc ttgattcaa 
      aacgccccg tatgaaagc acgcaattt ctgaaacgc 
      ttatgcacc aactacaac tcgcacaac tcattggac 
      aactccctc gttggtagt ttcgagctg tcgcaaaaa 
      tcggacccg gaggattac atccactta ttcctaaat 
      aactctgaa cgtttggtc gaatttttg gaatacttg 
      atcggcgag ggctgcatt ctgagcacc cctgtgtac 
      aacacatta ctcgagcac tatttgcac gtttggggc 
      aacctggag aacgtctcc gaacgcaac aaatacgcg 
      caaaaaaca ctgaaattg ttacaaaac cctgacatt 
      aaatacgac aagagccag gcgttggtc gtgtgccac 
      atgcattcg ttcagtgag ggtattttg tacttgtac 
      gaggagcag aagttgtac cagcagatt ttgcggcac 
      catatcacc aaaaacgac acgagctcg attctggcg 
      tgttgtcgc agattcggg caccaggag cccaccctg 
      tgggtgcaa gcgctgtgg tcgtgtgtg cgtgacacc 
      aaaaaccca ccaattgat ttactgaac gagattttg 
      accgtcatt gcgaaagag cgcttgctg tcgccccag 
      ctggtcgtg gacgcgctg gggacgggc agtgtggac 
      ataacactg gggcacatc cggtcgtat ctaacgaac 
      gagttgcaa caagagcag aaaaaaacg aaggagatc 
      tcagatttg acacataat tatcgcaag gatacggag 
      aagttgaag gagcagttg gagacgttg aaaagtggg 
      acgattgtg attcaaggc tcgcgctgt gcggcgtgt 
      caccacccg ttggagttg ccaactatt catttcctg 
      tgccagcac agctaccac cagcactgc ttccagagc 
      ttctgcgag gaggagaac gagtgtccg gcttgccag 
      cccgagaat aagaatttg ttggagttg ttgaaagct 
      cgtgagtat aataaggat ttgcatgag acgtttcac 
      tcgcagctg gagaaggcg caagatggg ttttcagtc 
      gcagcggag tatttcgga cgcggggtt tttaataag 
      tacaaagtg atcagagat gaggtacgt gagactgct 
      cccgccgat aaaattgaa aagaagcgt gttgttgag 
      cctgaggtt aggaattac gggctgggg gccgaagct 
      aggctgaga caggtggaa aaacagggg caggttttg 
      atcccgacg tctgaaggg agggtgcgc gtgcaggag 
      cataggtat agttcgtcc ctggaggcg aaaaatagg 
      agggtgcgc gtgcaggag cataggtat agttcgtcc 
      ctggaggcg aatattagt aactacgta ccaaagagt 
      tatgagagg aagagggag tacagtggg gatattgcg 
      ggcacgaac ccgttcgaa agtgattat gatgagagc 
      aagaacccg tttgctagt gaggatgat gactacgat 
      gagaataat ccctttcgg gacgattgt gataagaat 
      tcaaatccg ttttataaa taa
    mRNA sequence
    • >TC034845 RA
      gctgtcaaa gcaacaaaa atgttgggt tttgttaaa 
      taagagaaa aaacgttgt aaaatggcc tttttggag 
      tggcgcaag tttcatttc tttgacctg aaaaaagac 
      gtggacgag gggaaaata gccgagctt tttaaagaa 
      tctaaagtc acggtaaca tgtagcggt aacaaccac 
      attgttttg ggcgattca aacggccaa attttcctg 
      tgttctcgc tcgtggcat gtccgcact ttccgggcc 
      tatgagcta acagtagag ctcgcccac cagttgaga 
      aactcccct cttttggtc acaattggg caagatgag 
      actgggatt aatcccttg attaaagtc tgggacacg 
      agcagattt gacaaaaat gggacgccg ttttgttgc 
      cgcatttcg cgggcaatt cccgggaac cggcccgtg 
      cacgcgtca tgtctgtgt gtccatgag ggggcgcag 
      ttaatggcc gttgggttt gtcgatggt tctttggtc 
      ttgtatcgg ggtgatatc acgcgtgac cggagctcc 
      aaacaaaaa ctcttgcgt gacgcaagc tctactgtg 
      accggtctt gcattcaaa acgacttca accaacatt 
      tttttattc ctggctact gatagcgct gttatggtt 
      tataatatt actcataaa gataaagaa gtgaagttt 
      catttggat aacatcgga tgtgcgaaa aaatgcagc 
      gttttggcc gaatcgatg attgagagt catttcatg 
      gtcggccga aatgatgct atttattgc tacacggcc 
      gatggtcgc ggcccttgc tatgccgta gacggcgaa 
      aaagtcatg ttagagtgg tttcggagc taccttgtc 
      attatctca aaaacaaca cgaccaaac ctcgccata 
      acaaacgac aatcaaagt gcccccagc caaggcgac 
      ctgatcaca gttttagac attcacaac aaatttatc 
      gtattttcg gcaactgtt tcatcaatc cggtcagtt 
      ttaaatgaa tggggggcc ttctacatc ctcgataac 
      gacaacaga ctttaccac ctggacgag aaagacctc 
      caatcgaaa ttatcgctc cttttcaag aaaaattta 
      tacgacgtt gcaatccgt atcgcaaag tcgcaacag 
      tacgattcc gacggcctt gtcaacatt ttccggcaa 
      tacggcgac catttatgc gacaagggt gactatgtg 
      ggggcgatc gaacagtac ataaagaca atcgggaaa 
      ttggagccc agttatgtc attcggaaa tttctggac 
      tcgcaacac atcgaaaaa ttaacaatg tatctggaa 
      gccttacac aagcaaggc cacgccacc gaagaccac 
      acaaccctc ctcttgaac tgctacaca aaactgaac 
      aacacggtc ggacaatcg aacagtttg aaggagttt 
      attttgatg aaagagggc gatttgaac tacgacgta 
      gacattgcg attaaagtg tgccggcaa gggagccca 
      gctgaggcc ctaatgttg gccaaaaag cacgaaaaa 
      cacgactgg tacatcaaa ctacaaatc gaagaccac 
      caaaaatac gtggacgtg ctggactac atttcgaac 
      ctaactttc gaaaacgcc gaattttac atgaaaaaa 
      tacggcaat atcttgatt caaaacgcc ccgtatgaa 
      agcacgcaa tttctgaaa cgcttatgc accaactac 
      aactcgcac aactcattg gacaactcc ctcgttggt 
      agtttcgag ctgtcgcaa aaatcggac ccggaggat 
      tacatccac ttattccta aataactct gaacgtttg 
      gtcgaattt ttggaatac ttgatcggc gagggctgc 
      attctgagc acccctgtg tacaacaca ttactcgag 
      cactatttg cacgtttgg ggcaacctg gagaacgtc 
      tccgaacgc aacaaatac gcgcaaaaa acactgaaa 
      ttgttacaa aaccctgac attaaatac gacaagagc 
      caggcgttg gtcgtgtgc cacatgcat tcgttcagt 
      gagggtatt ttgtacttg tacgaggag cagaagttg 
      taccagcag attttgcgg caccatatc accaaaaac 
      gacacgagc tcgattctg gcgtgttgt cgcagattc 
      gggcaccag gagcccacc ctgtgggtg caagcgctg 
      tggtcgtgt gtgcgtgac accaaaaac ccaccaatt 
      gatttactg aacgagatt ttgaccgtc attgcgaaa 
      gagcgcttg ctgtcgccc cagctggtc gtggacgcg 
      ctggggacg ggcagtgtg gacataaca ctggggcac 
      atccggtcg tatctaacg aacgagttg caacaagag 
      cagaaaaaa acgaaggag atctcagat ttgacacat 
      aattatcgc aaggatacg gagaagttg aaggagcag 
      ttggagacg ttgaaaagt gggacgatt gtgattcaa 
      ggctcgcgc tgtgcggcg tgtcaccac ccgttggag 
      ttgccaact attcatttc ctgtgccag cacagctac 
      caccagcac tgcttccag agcttctgc gaggaggag 
      aacgagtgt ccggcttgc cagcccgag aataagaat 
      ttgttggag ttgttgaaa gctcgtgag tataataag 
      gatttgcat gagacgttt cactcgcag ctggagaag 
      gcgcaagat gggttttca gtcgcagcg gagtatttc 
      ggacgcggg gtttttaat aagtacaaa gtgatcaga 
      gatgaggta cgtgagact gctcccgcc gataaaatt 
      gaaaagaag cgtgttgtt gagcctgag gttaggaat 
      tacgggctg ggggccgaa gctaggctg agacaggtg 
      gaaaaacag gggcaggtt ttgatcccg acgtctgaa 
      gggagggtg cgcgtgcag gagcatagg tatagttcg 
      tccctggag gcgaaaaat aggagggtg cgcgtgcag 
      gagcatagg tatagttcg tccctggag gcgaatatt 
      agtaactac gtaccaaag agttatgag aggaagagg 
      gagtacagt ggggatatt gcgggcacg aacccgttc 
      gaaagtgat tatgatgag agcaagaac ccgtttgct 
      agtgaggat gatgactac gatgagaat aatcccttt 
      cgggacgat tgtgataag aattcaaat ccgttttat 
      aaataagtg attgtactc attataact aataaaaac 
      attctaatt acgtaattt acggattgc ctgggaaag 
      gttctgtcc tgtttttgc gcaacacga actgaatct 
      tccggtcca tgtcttcgt tgtaaatgg ctttgcact 
      tttcaaaat tgatgcgtc tacgaaact gaagggatt 
      ttacttttg gaaggcaag tttcagcaa ttacctgga 
      agggttttt cgaaaaaac gcgtccggc ggtattgct 
      ccaaaattc cctcccggc ctaatatcc tgttcagga 
      cacgacaag cagcctcaa cgtcgttat tttggacga 
      aaactgtcc gtgcaatga aagatgcgt gtctcatct 
      tgtcttaca gttacttga caagtgagg ttatgtaac 
      tcactctca aatttgatt tttcacgaa atccaaagg 
      agtttaatt aattgagtc aattttgtg cttagttta 
      attagcact tgataattt taaatttct ataacaggt 
      actgacctt ttccttgaa aaattatta atatgtaaa 
      aaattgatt ggtgctttg gccgtcacg tgacctgac 
      ttcgcctca cgattgggc aaaa
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