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Gene information
General information
TC005587 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10989451..10994430
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005587
    iBeetle screen iB_09377

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    larva segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva bristle pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax segment unilateral potentiallyfused - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs sometimes number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs structure irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005587 RA
      MVWQKPLIS PPTTTQHPN RPSGPAKAP IFRTTFHEG 
      DPTGGESTE SCDENDNMT DCRHVRQAV DLQKVKKAL 
      IKTGLTTEC EQCKKMPKV ASEMEPEFE FDNSLWLCL 
      RCGNQACGR NKNMHALEH FKTPHSDSH AICVDTTNW 
      SVWCYDCDE VVNATCKKK LLEAVEYLQ KLAEGKSNA 
      LPKPAEHQV MDLVPVTSS MSFGNMFNF TASNVPRPR 
      GLMNLGNTC FFNSVVQCL AQTPYLLNL LQETSEPGQ 
      FFQLPGGKL NPQDSDSPV LEPLTGELE KWRPLMATL 
      AETIVEVNN GRSEAYVPR LLLSKLTAR MPQFGGGNQ 
      HDSHELLRH LLEAVREED LRRYQSVIL DRLGLNTKT 
      DPSTVEGEK KKIIKFYGQ QASDLLLPT EQVFRGVLV 
      STLQCQECQ HTSHRDEFF LDLSLPITE KQLPPVLRR 
      KAEEIEDNK PSRHQIKKE KRAERKKNK KQKSHKNVN 
      IVMGPSNQP TAGLDVDMD KSDSESDAD VEDNVEVSE 
      EVTKGMESG YNSDKVDNS SPDSNNRGI SPEMRIDDS 
      GVPSPAIGM LSASHGTPD NSPASSETN IDMGSPLID 
      HCSPEEDGN EYFDRPESR LAFVNNKNV DLKTGLSKL 
      SLLNDGDSS KVGTFCNDK MDDDDNFEG ACGGEDPKD 
      EKMDEDDED VDLWTNTMS ARYQCEEGE YSVQSCLNQ 
      FTACELMTG NNKVSCELC TKRHGGPDK KTVYTNATK 
      QLLIYNPPA VLILHLKRF QVYRFRSAK VPKFVKFPT 
      LLDLAPFCS KRSQNLPTF EAGQTKVLY SLYGVVEHS 
      GSIHGGHYV AYVKVRPKL EENSYRWQF LPKNQKNEK 
      GQAPKGAQG EPEVPSGKW YYISDSHVS EASETRVLS 
      AQAYLLFYE RIL
    CDS sequence
    • >TC005587 RA
      atggtgtgg caaaaaccg ctgatatcg ccgcccacg 
      accacgcaa cacccgaat cgcccttcc ggccccgca 
      aaagcgccc atttttcgc accacattt catgaaggc 
      gaccccacg gggggcgaa tcgaccgaa tcctgcgac 
      gagaacgac aacatgacg gattgccgt cacgtccgc 
      caggccgtg gacttgcaa aaggtcaag aaggcccta 
      atcaagacc ggactgacc accgaatgc gagcaatgc 
      aagaagatg cccaaagtc gcgtccgaa atggaaccc 
      gaattcgag ttcgataac tcgctatgg ctgtgtttg 
      cggtgcggg aaccaggcc tgcggccgg aacaaaaac 
      atgcacgcc ttggagcac ttcaagact ccgcactcg 
      gactcgcac gccatttgc gtcgatacg accaactgg 
      agtgtctgg tgttatgac tgtgatgaa gtcgtcaat 
      gcgacttgc aagaaaaaa ctgctggaa gccgtcgaa 
      tacctccag aagctggcc gagggcaag agcaacgcg 
      ctgcccaaa cccgccgag caccaagtg atggattta 
      gtcccggta acttcgagc atgagtttt gggaacatg 
      ttcaacttc accgcttcg aacgtacct cgaccgcgt 
      ggcttaatg aacctaggc aatacgtgt ttctttaat 
      tcagtagtt caatgttta gctcaaacg ccctatttg 
      ttaaacctt ttacaagaa acctccgaa ccggggcag 
      tttttccaa ctaccgggg ggcaagttg aacccccag 
      gatagcgac tccccggta cttgagccc ttgaccggg 
      gagttggag aagtggcgc ccgcttatg gccacgttg 
      gccgaaact atagtcgaa gtgaacaat ggacgcagt 
      gaagcatac gttcctcgt ttacttctc tccaaacta 
      accgctcga atgccgcag tttggcggc ggcaaccaa 
      cacgactcg cacgagctc ctgcgccac ttactcgag 
      gccgtgcga gaggaggac ttgcgccgc taccaatcg 
      gttatcctc gaccggtta ggtttgaac acgaaaacc 
      gacccgagc acggtcgag ggtgagaag aagaaaata 
      atcaagttt tacggccaa caagcttcc gatctattg 
      ctaccaacc gaacaagtt ttccgaggg gtgcttgtt 
      agcacgctg cagtgccag gagtgccaa cacacctcg 
      caccgcgac gagtttttc ctagattta agtctaccg 
      atcactgaa aaacaacta ccgccggtt ttgcgacgc 
      aaagccgag gaaatcgag gataataaa ccgtcacgc 
      caccagatc aagaaggag aaacgtgcg gaaagaaag 
      aaaaacaag aagcaaaag tcgcataaa aacgtcaat 
      attgtcatg gggcccagc aaccagccc acggccggg 
      cttgacgtg gacatggat aagtcggat agtgagtcg 
      gacgccgac gttgaggac aacgtcgag gtgtccgag 
      gaggtgacc aaagggatg gagtcgggg tataattcg 
      gacaaggtc gataacagc agccccgac tcgaacaat 
      cgcggtatt tcccccgaa atgcgcatt gacgacagt 
      ggggttccc agtcccgcg attgggatg cttagtgcg 
      tcgcacggc accccggac aatagcccg gcctcgagt 
      gagaccaac atcgacatg gggagcccc cttatcgat 
      cattgtagt cccgaagaa gacggtaat gaatatttc 
      gacaggcct gagtcgaga ctggccttc gtgaacaac 
      aaaaacgtc gatttgaag acaggtttg agtaaatta 
      agtctactg aacgatggt gattcgagt aaagttggt 
      acgttttgc aacgataag atggacgat gatgataat 
      ttcgaaggt gcctgtggg ggcgaggat ccaaaggat 
      gaaaagatg gatgaggac gacgaagac gtggacctg 
      tggaccaac acgatgtcg gcgcgttac cagtgcgaa 
      gaaggcgaa tactctgtc caatcctgc ctcaaccag 
      ttcacagct tgcgaactg atgaccggt aacaacaaa 
      gtcagctgc gagctgtgc acgaagcgc cacggtggc 
      cccgataag aaaaccgtc tacaccaac gccaccaag 
      cagctcctt atctacaac ccccctgcc gttctaatc 
      ctgcacttg aagcgtttc caagtgtac cgatttcgc 
      tccgccaaa gtccccaaa ttcgtcaag ttccccacg 
      cttctcgat ctcgcgccg ttctgttcg aaacgctcc 
      caaaacctc ccgactttc gaggccggt cagacgaaa 
      gtcttgtac tctttatac ggggttgtg gagcatagc 
      gggtccatt cacgggggc cactacgtg gcctacgtg 
      aaagtgcgg cccaagttg gaggagaac agctatagg 
      tggcagttt ttgcccaaa aatcagaaa aacgagaag 
      gggcaggcg cccaagggg gcgcaaggc gagcccgag 
      gtgccctcc gggaagtgg tactatatc agcgattcg 
      cacgtttcc gaagccagc gagacgcgg gttttgagc 
      gcgcaagcc tatttgctg ttctatgag aggatcttg 
      taa
    mRNA sequence
    • >TC005587 RA
      ggaatggtg tggcaaaaa ccgctgata tcgccgccc 
      acgaccacg caacacccg aatcgccct tccggcccc 
      gcaaaagcg cccattttt cgcaccaca tttcatgaa 
      ggcgacccc acggggggc gaatcgacc gaatcctgc 
      gacgagaac gacaacatg acggattgc cgtcacgtc 
      cgccaggcc gtggacttg caaaaggtc aagaaggcc 
      ctaatcaag accggactg accaccgaa tgcgagcaa 
      tgcaagaag atgcccaaa gtcgcgtcc gaaatggaa 
      cccgaattc gagttcgat aactcgcta tggctgtgt 
      ttgcggtgc gggaaccag gcctgcggc cggaacaaa 
      aacatgcac gccttggag cacttcaag actccgcac 
      tcggactcg cacgccatt tgcgtcgat acgaccaac 
      tggagtgtc tggtgttat gactgtgat gaagtcgtc 
      aatgcgact tgcaagaaa aaactgctg gaagccgtc 
      gaatacctc cagaagctg gccgagggc aagagcaac 
      gcgctgccc aaacccgcc gagcaccaa gtgatggat 
      ttagtcccg gtaacttcg agcatgagt tttgggaac 
      atgttcaac ttcaccgct tcgaacgta cctcgaccg 
      cgtggctta atgaaccta ggcaatacg tgtttcttt 
      aattcagta gttcaatgt ttagctcaa acgccctat 
      ttgttaaac cttttacaa gaaacctcc gaaccgggg 
      cagtttttc caactaccg gggggcaag ttgaacccc 
      caggatagc gactccccg gtacttgag cccttgacc 
      ggggagttg gagaagtgg cgcccgctt atggccacg 
      ttggccgaa actatagtc gaagtgaac aatggacgc 
      agtgaagca tacgttcct cgtttactt ctctccaaa 
      ctaaccgct cgaatgccg cagtttggc ggcggcaac 
      caacacgac tcgcacgag ctcctgcgc cacttactc 
      gaggccgtg cgagaggag gacttgcgc cgctaccaa 
      tcggttatc ctcgaccgg ttaggtttg aacacgaaa 
      accgacccg agcacggtc gagggtgag aagaagaaa 
      ataatcaag ttttacggc caacaagct tccgatcta 
      ttgctacca accgaacaa gttttccga ggggtgctt 
      gttagcacg ctgcagtgc caggagtgc caacacacc 
      tcgcaccgc gacgagttt ttcctagat ttaagtcta 
      ccgatcact gaaaaacaa ctaccgccg gttttgcga 
      cgcaaagcc gaggaaatc gaggataat aaaccgtca 
      cgccaccag atcaagaag gagaaacgt gcggaaaga 
      aagaaaaac aagaagcaa aagtcgcat aaaaacgtc 
      aatattgtc atggggccc agcaaccag cccacggcc 
      gggcttgac gtggacatg gataagtcg gatagtgag 
      tcggacgcc gacgttgag gacaacgtc gaggtgtcc 
      gaggaggtg accaaaggg atggagtcg gggtataat 
      tcggacaag gtcgataac agcagcccc gactcgaac 
      aatcgcggt atttccccc gaaatgcgc attgacgac 
      agtggggtt cccagtccc gcgattggg atgcttagt 
      gcgtcgcac ggcaccccg gacaatagc ccggcctcg 
      agtgagacc aacatcgac atggggagc ccccttatc 
      gatcattgt agtcccgaa gaagacggt aatgaatat 
      ttcgacagg cctgagtcg agactggcc ttcgtgaac 
      aacaaaaac gtcgatttg aagacaggt ttgagtaaa 
      ttaagtcta ctgaacgat ggtgattcg agtaaagtt 
      ggtacgttt tgcaacgat aagatggac gatgatgat 
      aatttcgaa ggtgcctgt gggggcgag gatccaaag 
      gatgaaaag atggatgag gacgacgaa gacgtggac 
      ctgtggacc aacacgatg tcggcgcgt taccagtgc 
      gaagaaggc gaatactct gtccaatcc tgcctcaac 
      cagttcaca gcttgcgaa ctgatgacc ggtaacaac 
      aaagtcagc tgcgagctg tgcacgaag cgccacggt 
      ggccccgat aagaaaacc gtctacacc aacgccacc 
      aagcagctc cttatctac aacccccct gccgttcta 
      atcctgcac ttgaagcgt ttccaagtg taccgattt 
      cgctccgcc aaagtcccc aaattcgtc aagttcccc 
      acgcttctc gatctcgcg ccgttctgt tcgaaacgc 
      tcccaaaac ctcccgact ttcgaggcc ggtcagacg 
      aaagtcttg tactcttta tacggggtt gtggagcat 
      agcgggtcc attcacggg ggccactac gtggcctac 
      gtgaaagtg cggcccaag ttggaggag aacagctat 
      aggtggcag tttttgccc aaaaatcag aaaaacgag 
      aaggggcag gcgcccaag ggggcgcaa ggcgagccc 
      gaggtgccc tccgggaag tggtactat atcagcgat 
      tcgcacgtt tccgaagcc agcgagacg cgggttttg 
      agcgcgcaa gcctatttg ctgttctat gagaggatc 
      ttgtaagct ttgaccgtg ctgtttatt ttagcttct 
      ttatacata taattattg ttttggaat tgatttttg 
      ggtcttacg gtggaattt tattgagaa attttatca 
      tttcaatcc gaaatagcc gcttgataa ttgtgagga 
      gattgacag ttttttaga tttattgtt acattgtaa 
      gcgttttct gggttcatt atgtaaact gtaacacct 
      cttttggtc tgtgcatac tagtgtaat tattttttt 
      aagcgattt ctccgaatg tattcagca aattaaata 
      aactgtaaa ggacgag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY