Status
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Gene information
General information
TC005594 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10886499..10891393
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005594
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09375

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005594 RA
      MEPIAPGTE DEPLVNFSA STKQEKERE RLLNVKTVL 
      PNGKYGNVF DFKYINGTE HWYCNACAC PVMGWVFHH 
      ETGKRHSLA LGKQNQDQD RLTSSQDVE ETPTIQIAP 
      GEPVPPGFE GEIERVALI QERLDVFNV GPLVALEYL 
      LELRDYDSN KEPTYLCIL CDKKGDPRT VLTHLASYK 
      HILQYLQKH FPTCYRALA PYMTKQYKR NWQIALHEI 
      AEAIEKKFG RLKPLPVEA DKYEKDPVH YLELIAKGR 
      HFSELSGHT FEELVNKDE LTRVHDDET DKFSYNKAN 
      LPQSSQKKK SPSPPVVAN PIKKIRVAN PPPAAAGSR 
      RRSLSSVSS VSSSDLSDY DDPKKRSYS RRSRTRSPP 
      YRNRSRSPY SRRYDRRSP PRKEAYYRR DDRSERLMP 
      WEKTNYRRS KPENPPVKN KTDKMDEYK KLCKAIDND 
      MERVLKNHK KNPEKHPQY NDEWKKFWN KRYKELQAE 
      GKDVSTHDF KPEWIEFWN KRMIELHHE EVKVKKEAL 
      RKRLGLPEE PSPISFKIV GKKKFEPNK NSPMSSKPV 
      PPSALPDSD PEVIIIDKD DESKSSRRS HSPWEDEPV 
      RSSSRVSRD KSRDGSRDR SRERSIRRD KYSRSPRDR 
      SRERSWDRD YKIKDYRRE RIRIVSELP WERDRKSYR 
      NEIPSYYKP PAVMRDVTR EPVLTPPPV APVLEEEDD 
      GEINIVAVL RLLTALEEK LGSLGPKVI DLLAQALAM 
      EKNEANSSE NLLDNEINC VMFETVKEK LKGQLLAGL 
      VEPIQEKAF KNAIKKTAS LIHLAGERK KEKAKNQPK 
      VDPVKVPGV GTVDKAAIA KQIANALIL QGKTDVTQA 
      ELEQLINAV VGMAEASRN SNKPITTAS FLEQISKAG 
      SSKEKTPPK EVVKPPAPV VAERVTKSP EKMTNKDME 
      GLSDSDLQT LLQNFKDLS TEEQHSLIN YLKKLEARE 
      PDRVERLRK FVNLGQPRE KPPEEKKMG RESPFSNRM 
      GSVNPSVEE NRFEAEEEP KKFEETQVK LPIDSDEED 
      YSFEDVVKA ASKNVKEKE LEKKREIVE ESMKFESKK 
      EDLNLDDTK AIISNIMSN INKNEVAQG NLLGLGLGG 
      GQGLSVSSA DLAKTLNSI PVNMASLAN IVGSVQSMT 
      KATMAPSVN PPMSAAPKS FQPVQSNSF GGFGGPQER 
      PFQGQQQGY QNRPNMQFG VNYPQNPYQ ARPNVAYPI 
      DKNKVTYPN YAIYGQQNP QGPPQRPSQ PGFGNNQFG 
      NNVNRNNYS RW
    CDS sequence
    • >TC005594 RA
      atggaaccc atcgctccg ggcactgaa gatgagcct 
      cttgttaat ttttcagca tcgacgaaa caggagaag 
      gaacgcgag cgtttgctg aacgtgaaa actgttcta 
      ccgaacgga aaatatggg aacgttttc gactttaaa 
      tacatcaac gggacggaa cattggtac tgcaatgct 
      tgcgcctgt ccagtcatg ggatgggtt ttccaccat 
      gagactgga aagcgccac tccttggcc ctcgggaag 
      caaaaccaa gaccaagac aggctcacg tcatcgcag 
      gacgtggag gaaacaccc accatccag atagcgccc 
      ggagagcca gtccctcca ggatttgag ggagaaatt 
      gaacgagtc gctcttatt caagaacgg ttggatgtc 
      tttaatgtt ggtccctta gttgctctg gaatacctc 
      ctagaattg cgcgattac gactcaaac aaggaaccg 
      acttatttg tgcattttg tgcgacaaa aaaggcgac 
      ccgaggacc gttttgacg caccttgcg agttacaaa 
      cacatactg cagtatttg caaaaacac tttccgact 
      tgttaccgg gccctggcc ccttatatg accaaacag 
      tataaaaga aactggcaa atagcccta cacgagatc 
      gccgaagca attgagaaa aagtttggc cgtttgaaa 
      ccgttacca gtggaagca gacaagtat gagaaagac 
      cccgtccat tatttagaa ctgatagcc aaaggtcgg 
      cacttttcc gaattgtca ggtcatacg ttcgaggaa 
      ttggtcaat aaagacgaa ctgacgcga gtccatgat 
      gacgagacc gataaattt tcttataat aaggcaaat 
      ttgccacag tcaagtcag aaaaagaaa agtccgtca 
      cctcctgtt gtcgcaaat ccgatcaag aaaattcga 
      gttgctaat ccaccaccg gctgctgcc ggaagtcgc 
      cgaaggtca ctgtcgagt gtttcgagc gtttcttca 
      agcgatctt agcgactat gatgacccc aaaaaacgg 
      tcgtatagt cgcaggtcg aggacaagg tcgcccccg 
      taccgaaac cgcagccga agtccctac agccgaaga 
      tatgatagg cgatcgccc ccgaggaag gaagcttat 
      tacagacga gacgaccga agtgagcgc cttatgccg 
      tgggaaaag acgaattat cgccgctcc aagcccgaa 
      aacccaccg gttaaaaac aaaaccgat aaaatggac 
      gagtacaag aaattatgc aaggccata gacaacgac 
      atggagcgg gttttgaaa aaccataaa aagaacccc 
      gaaaaacac ccccaatac aatgatgag tggaaaaaa 
      ttctggaat aagaggtac aaggagctc caagctgaa 
      ggcaaggac gtctccact catgatttc aaaccggaa 
      tggatcgag ttctggaac aaacgcatg atcgagctg 
      caccacgaa gaagtcaaa gtgaagaaa gaagctttg 
      cggaaacgt ctaggcctt cctgaagag ccctctccc 
      attagtttt aaaatcgtc ggaaaaaag aaattcgaa 
      ccgaataaa aacagtcca atgagtagt aaaccagtg 
      ccaccttca gctttgccc gatagtgat cccgaggtt 
      attataatc gataaagat gatgagagt aagagttcg 
      cgacggtcg catagtcca tgggaggat gaaccagtg 
      aggagtagt tcccgggtt tcgcgtgac aaaagtcgg 
      gacggctcc agggacagg agtcgggaa cgctcaata 
      cgacgggat aaatacagt cgaagtccc agagaccgg 
      tcacgggag agaagttgg gaccgggat tacaagatc 
      aaggattat cgaagggaa cggattaga atcgtcagt 
      gaattgccg tgggagcgc gaccggaag tcctacagg 
      aacgaaatc cctagctac tacaaacca ccagctgtc 
      atgcgtgac gttacaaga gaacctgtc ttgacgccc 
      cctcctgtt gccccagtc cttgaagaa gaggacgat 
      ggggaaatt aatatcgtt gctgttctg cgactttta 
      acagcactt gaagagaaa ttagggtca ttgggaccc 
      aaagtcatc gatttgtta gcacaagca ctggcgatg 
      gagaagaac gaagctaat agttcggag aatttgctc 
      gataatgag atcaattgt gtgatgttt gaaacggtc 
      aaagaaaaa ttaaaaggc cagctgttg gccggttta 
      gtcgaaccc attcaggaa aaagccttt aaaaacgca 
      attaaaaaa accgcgagt ttgattcat ctcgccggc 
      gaacgcaag aaagaaaaa gccaagaat caaccgaaa 
      gttgatccc gttaaagtg ccaggtgtt ggcacagtc 
      gacaaggct gcgattgca aaacaaatc gccaatgcg 
      ttaattctg caaggcaag actgacgtg acccaagcc 
      gagctcgaa cagttgata aatgccgtg gttggaatg 
      gcggaagcg tcccgaaat tccaataaa ccgatcacc 
      accgcctct ttcctggaa caaatctcc aaagctggc 
      agttccaaa gagaaaaca ccaccgaaa gaagttgtt 
      aaacccccg gcaccggtt gtggccgaa cgcgtgacg 
      aaaagcccc gagaaaatg acgaataaa gacatggag 
      ggtttgtcc gattccgat ttgcaaact ctcctccaa 
      aacttcaaa gatttatcg acggaagaa cagcacagt 
      ttgatcaac tatttgaag aagttggaa gcgcgtgaa 
      ccggatcga gtcgagcgg ttacgaaag ttcgtaaat 
      ctgggccaa ccgcgtgaa aagcccccc gaagagaag 
      aaaatgggg cgcgaaagc cccttttcc aaccgcatg 
      gggagtgtg aaccccagt gttgaggaa aatcgcttt 
      gaagcggaa gaagaaccg aaaaagttc gaagagacg 
      caagtgaaa cttccgatt gattcggac gaagaggat 
      tacagtttc gaggacgtg gtcaaagcc gcgtccaag 
      aacgtcaaa gagaaggaa ctggagaag aagcgcgag 
      atcgtggag gaaagcatg aagtttgag tcgaagaaa 
      gaggacctg aatctggac gataccaaa gccataatc 
      tccaacatc atgagtaac attaacaag aacgaggtc 
      gcgcaaggt aatctcttg gggctgggg ctgggtggg 
      gggcaaggt ctgtcggtg agttcggcc gatttggcc 
      aaaaccctg aatagtatt ccggtgaat atggccagt 
      cttgccaac attgttggc agtgtacag agtatgaca 
      aaggccact atggcaccg tctgtaaac ccgccaatg 
      agcgccgcg cctaagagt tttcagccg gtgcaaagt 
      aacagtttt ggggggttt gggggcccg caggagaga 
      ccctttcag ggacagcag cagggatac caaaataga 
      cctaacatg cagtttggg gtgaattac ccccaaaac 
      ccatatcaa gcgcggcca aatgttgcc tacccgatt 
      gataaaaac aaggtgacg tatccgaat tatgccatt 
      tacggccaa cagaacccg caagggccg ccccagagg 
      ccgagtcag cccgggttt ggcaataac cagttcggt 
      aataatgtt aacagaaat aactatagc agatggtag
    mRNA sequence
    • >TC005594 RA
      ttggtggtg caccaataa atgtcaagc cacgaaaaa 
      ttgtcttgg aaaaaatgt tctaatttg attaaaatt 
      gaatctcct tgtgggttc tgagcttcc taatcagta 
      gacaagtga tttagttaa gaagtgttt agtgaattg 
      tgatatgga acccatcgc tccgggcac tgaagatga 
      gcctcttgt taatttttc agcatcgac gaaacagga 
      gaaggaacg cgagcgttt gctgaacgt gaaaactgt 
      tctaccgaa cggaaaata tgggaacgt tttcgactt 
      taaatacat caacgggac ggaacattg gtactgcaa 
      tgcttgcgc ctgtccagt catgggatg ggttttcca 
      ccatgagac tggaaagcg ccactcctt ggccctcgg 
      gaagcaaaa ccaagacca agacaggct cacgtcatc 
      gcaggacgt ggaggaaac acccaccat ccagatagc 
      gcccggaga gccagtccc tccaggatt tgagggaga 
      aattgaacg agtcgctct tattcaaga acggttgga 
      tgtctttaa tgttggtcc cttagttgc tctggaata 
      cctcctaga attgcgcga ttacgactc aaacaagga 
      accgactta tttgtgcat tttgtgcga caaaaaagg 
      cgacccgag gaccgtttt gacgcacct tgcgagtta 
      caaacacat actgcagta tttgcaaaa acactttcc 
      gacttgtta ccgggccct ggcccctta tatgaccaa 
      acagtataa aagaaactg gcaaatagc cctacacga 
      gatcgccga agcaattga gaaaaagtt tggccgttt 
      gaaaccgtt accagtgga agcagacaa gtatgagaa 
      agaccccgt ccattattt agaactgat agccaaagg 
      tcggcactt ttccgaatt gtcaggtca tacgttcga 
      ggaattggt caataaaga cgaactgac gcgagtcca 
      tgatgacga gaccgataa attttctta taataaggc 
      aaatttgcc acagtcaag tcagaaaaa gaaaagtcc 
      gtcacctcc tgttgtcgc aaatccgat caagaaaat 
      tcgagttgc taatccacc accggctgc tgccggaag 
      tcgccgaag gtcactgtc gagtgtttc gagcgtttc 
      ttcaagcga tcttagcga ctatgatga ccccaaaaa 
      acggtcgta tagtcgcag gtcgaggac aaggtcgcc 
      cccgtaccg aaaccgcag ccgaagtcc ctacagccg 
      aagatatga taggcgatc gcccccgag gaaggaagc 
      ttattacag acgagacga ccgaagtga gcgccttat 
      gccgtggga aaagacgaa ttatcgccg ctccaagcc 
      cgaaaaccc accggttaa aaacaaaac cgataaaat 
      ggacgagta caagaaatt atgcaaggc catagacaa 
      cgacatgga gcgggtttt gaaaaacca taaaaagaa 
      ccccgaaaa acaccccca atacaatga tgagtggaa 
      aaaattctg gaataagag gtacaagga gctccaagc 
      tgaaggcaa ggacgtctc cactcatga tttcaaacc 
      ggaatggat cgagttctg gaacaaacg catgatcga 
      gctgcacca cgaagaagt caaagtgaa gaaagaagc 
      tttgcggaa acgtctagg ccttcctga agagccctc 
      tcccattag ttttaaaat cgtcggaaa aaagaaatt 
      cgaaccgaa taaaaacag tccaatgag tagtaaacc 
      agtgccacc ttcagcttt gcccgatag tgatcccga 
      ggttattat aatcgataa agatgatga gagtaagag 
      ttcgcgacg gtcgcatag tccatggga ggatgaacc 
      agtgaggag tagttcccg ggtttcgcg tgacaaaag 
      tcgggacgg ctccaggga caggagtcg ggaacgctc 
      aatacgacg ggataaata cagtcgaag tcccagaga 
      ccggtcacg ggagagaag ttgggaccg ggattacaa 
      gatcaagga ttatcgaag ggaacggat tagaatcgt 
      cagtgaatt gccgtggga gcgcgaccg gaagtccta 
      caggaacga aatccctag ctactacaa accaccagc 
      tgtcatgcg tgacgttac aagagaacc tgtcttgac 
      gccccctcc tgttgcccc agtccttga agaagagga 
      cgatgggga aattaatat cgttgctgt tctgcgact 
      tttaacagc acttgaaga gaaattagg gtcattggg 
      acccaaagt catcgattt gttagcaca agcactggc 
      gatggagaa gaacgaagc taatagttc ggagaattt 
      gctcgataa tgagatcaa ttgtgtgat gtttgaaac 
      ggtcaaaga aaaattaaa aggccagct gttggccgg 
      tttagtcga acccattca ggaaaaagc ctttaaaaa 
      cgcaattaa aaaaaccgc gagtttgat tcatctcgc 
      cggcgaacg caagaaaga aaaagccaa gaatcaacc 
      gaaagttga tcccgttaa agtgccagg tgttggcac 
      agtcgacaa ggctgcgat tgcaaaaca aatcgccaa 
      tgcgttaat tctgcaagg caagactga cgtgaccca 
      agccgagct cgaacagtt gataaatgc cgtggttgg 
      aatggcgga agcgtcccg aaattccaa taaaccgat 
      caccaccgc ctctttcct ggaacaaat ctccaaagc 
      tggcagttc caaagagaa aacaccacc gaaagaagt 
      tgttaaacc cccggcacc ggttgtggc cgaacgcgt 
      gacgaaaag ccccgagaa aatgacgaa taaagacat 
      ggagggttt gtccgattc cgatttgca aactctcct 
      ccaaaactt caaagattt atcgacgga agaacagca 
      cagtttgat caactattt gaagaagtt ggaagcgcg 
      tgaaccgga tcgagtcga gcggttacg aaagttcgt 
      aaatctggg ccaaccgcg tgaaaagcc ccccgaaga 
      gaagaaaat ggggcgcga aagcccctt ttccaaccg 
      catggggag tgtgaaccc cagtgttga ggaaaatcg 
      ctttgaagc ggaagaaga accgaaaaa gttcgaaga 
      gacgcaagt gaaacttcc gattgattc ggacgaaga 
      ggattacag tttcgagga cgtggtcaa agccgcgtc 
      caagaacgt caaagagaa ggaactgga gaagaagcg 
      cgagatcgt ggaggaaag catgaagtt tgagtcgaa 
      gaaagagga cctgaatct ggacgatac caaagccat 
      aatctccaa catcatgag taacattaa caagaacga 
      ggtcgcgca aggtaatct cttggggct ggggctggg 
      tggggggca aggtctgtc ggtgagttc ggccgattt 
      ggccaaaac cctgaatag tattccggt gaatatggc 
      cagtcttgc caacattgt tggcagtgt acagagtat 
      gacaaaggc cactatggc accgtctgt aaacccgcc 
      aatgagcgc cgcgcctaa gagttttca gccggtgca 
      aagtaacag ttttggggg gtttggggg cccgcagga 
      gagaccctt tcagggaca gcagcaggg ataccaaaa 
      tagacctaa catgcagtt tggggtgaa ttaccccca 
      aaacccata tcaagcgcg gccaaatgt tgcctaccc 
      gattgataa aaacaaggt gacgtatcc gaattatgc 
      catttacgg ccaacagaa cccgcaagg gccgcccca 
      gaggccgag tcagcccgg gtttggcaa taaccagtt 
      cggtaataa tgttaacag aaataacta tagcagatg 
      gtagactaa gtttttatc aaataacgt aaggctttt 
      ttatgttaa tttatgttt aagcgtagt tattctatg 
      aataaatag tttcattta aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY