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Gene information
General information
TC005616 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10595114..10596748
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005616
    iBeetle screen iB_09371

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005616 RA
      MATFFTHPL DLIKVHLQT HAGKKISII HLTTDIVKK 
      NGFLSLYNG LSASLCRQL TYSVIRFGI YDTAKLYME 
      KDSSLTSRI FVAFFAGSF GGFVGTPPD KVNVRMQND 
      VKLPPEKRF NYKHAFDGL WHVYQSEGF AKLFTGGGT 
      ASFRAGVMG VGQLTSYDQ IKRVLLRTS YFEDDLVTH 
      FTSSMGAAV IATTITQPL DVIKTRVMN AKPGEFRNI 
      LDVVLFTAK EGPLGFFKG YVPAFLRIG PHTIITFIF 
      YERLRMYFG YIPEPKVK
    CDS sequence
    • >TC005616 RA
      atggcgaca tttttcacc cacccgttg gatttaatc 
      aaagtgcat ttgcaaacc catgctggt aaaaaaata 
      tcgataata cacctcacc acagacatt gtgaaaaaa 
      aatggcttt ctaagtctc tacaacggt ctctcggct 
      tcactgtgc cgccagctc acttactct gtcatcaga 
      ttcggtatt tacgacact gcgaaactc tacatggaa 
      aaagacagc agtctcacg agccgtatt tttgtggcg 
      ttttttgca ggaagtttc ggcggtttt gtggggacg 
      ccccctgat aaagtcaac gttcggatg caaaacgac 
      gtaaaacta cccccggaa aaaagattc aattacaag 
      cacgccttt gacggcctt tggcacgtt tatcaaagc 
      gaaggcttt gcgaaactt ttcacaggg ggtggaact 
      gcgtcgttt cgagccggg gtcatgggg gtgggacag 
      ttaacttca tacgaccaa atcaaaaga gttctttta 
      agaacgagc tactttgaa gatgattta gttacgcat 
      tttacgtcc agtatgggt gctgccgtg attgccacg 
      accataact cagccttta gacgtgata aagacacga 
      gtaatgaac gcaaagcct ggcgaattt aggaatatt 
      ttggacgtt gtgttgttt acggcaaag gaggggccc 
      ttgggcttt ttcaagggt tacgtccct gcgttttta 
      aggatagga ccgcacacg atcataact tttattttt 
      tatgagcgc ttgaggatg tattttggg tacattccg 
      gaaccgaaa gtgaaataa
    mRNA sequence
    • >TC005616 RA
      ttctgacag agtcccacg atggtactt cggcggact 
      tgcctcggc aatggcgac atttttcac ccacccgtt 
      ggatttaat caaagtgca tttgcaaac ccatgctgg 
      taaaaaaat atcgataat acacctcac cacagacat 
      tgtgaaaaa aaatggctt tctaagtct ctacaacgg 
      tctctcggc ttcactgtg ccgccagct cacttactc 
      tgtcatcag attcggtat ttacgacac tgcgaaact 
      ctacatgga aaaagacag cagtctcac gagccgtat 
      ttttgtggc gttttttgc aggaagttt cggcggttt 
      tgtggggac gccccctga taaagtcaa cgttcggat 
      gcaaaacga cgtaaaact acccccgga aaaaagatt 
      caattacaa gcacgcctt tgacggcct ttggcacgt 
      ttatcaaag cgaaggctt tgcgaaact tttcacagg 
      gggtggaac tgcgtcgtt tcgagccgg ggtcatggg 
      ggtgggaca gttaacttc atacgacca aatcaaaag 
      agttctttt aagaacgag ctactttga agatgattt 
      agttacgca ttttacgtc cagtatggg tgctgccgt 
      gattgccac gaccataac tcagccttt agacgtgat 
      aaagacacg agtaatgaa cgcaaagcc tggcgaatt 
      taggaatat tttggacgt tgtgttgtt tacggcaaa 
      ggaggggcc cttgggctt tttcaaggg ttacgtccc 
      tgcgttttt aaggatagg accgcacac gatcataac 
      ttttatttt ttatgagcg cttgaggat gtattttgg 
      gtacattcc ggaaccgaa agtgaaata aaaaagtac 
      tttttcttc gatttatta caacataaa gatatacat 
      tttcaacac aaaataaaa atttgtact ggaatatta 
      aaaacgtta aaaacctta agtatcacg gcaagaaac 
      tgggagtaa atgcttaat taggtaaat gcatttgtt 
      tctaacttc tactcggat taataagta caggacatt 
      tattgacca aagtcactt tcataacgc aagggtgct 
      gctcccaag gctcaccat cgatctgaa cgggcgatt 
      tggacttaa gtcgtacct caactgttt ttgcttgac 
      ctagtttta gtggagtgg aaagaccaa cttgtagtt 
      gtgcaatat gaaaggaag agtaaattc ccaaaactt 
      ccaaaatac catcgtggt atgactgtg ccggctttg 
      tgttattcc ccaaaagac ctgaaacaa ctttgggca 
      caaaacact aaattgatg cgattttac cccataaat 
      tgacccccg ccccccatg atgggatgt ttaaaataa 
      caatcgact ccagttcgg gcaaatcga cttgtttcc 
      cgtccaaat acaagtcga ttttttgct ccaagtttt 
      tacaatctg acgtaacca cttgttgcg tcccgaaac 
      ataaataaa gtatctaca attca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY