Status
Please wait ...
Gene information
General information
TC005637 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 10421262..10423238
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005637
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09368

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005637 RA
      MSKIPENNS VKSEQPISQ TCCPGCLCN SEVKYEVCQ 
      TTKPHSIKS ARNLSTGEV IFRELPLIS GPRLDHKND 
      EKLCIGCCK LLSNENKIV CPNCSLPLC SLDCDGFSP 
      RHDFECSVF KLGQFDQNF KYGALLPLR TLFLQKTNT 
      VQWQKIFEQ CDVDFVLNR NDEIEYLVQ KFLTPLKKL 
      EEETHQIIL EDKSEETLG KIFNFLQSN THHNKQLIS 
      FYPNASLLK HSCTPNTIF TTENFEITL RAISGVSEG 
      TPLTCSYTD LFSGTHVRL KNLKKLNKT CFCARCTDP 
      TEFGTFFSA LKCMGTKQD PCGGNQLPV NPTNEETLW 
      VCDKCKIEL PHKQIVKFV KHLGGQVGK TLARKVSIE 
      ELEEFLGKL LMFLHPNHF YIFAVKNAL VELYGEESL 
      ATKIQICEE LIDIAGKFE DWGLRLGRL LHELFASKT 
      SNLVENKNV EELATTIAK MRKIAQVHQ DPDFLQIFL 
      EDESKLASL
    CDS sequence
    • >TC005637 RA
      atgtctaaa attcctgaa aataattcc gtaaagtct 
      gaacaacca atctcgcaa acgtgttgt ccaggttgt 
      ctttgcaat tctgaggta aaatatgag gtgtgccaa 
      acaactaaa ccccattcg attaaatcg gcaagaaat 
      ttgtccacc ggagaagta atttttagg gagttaccg 
      ctcatttct ggacctcga cttgaccac aaaaatgac 
      gaaaagctc tgcattggc tgttgcaaa cttctctct 
      aatgaaaat aaaattgtt tgccctaat tgttccttg 
      cctttgtgt agtcttgac tgtgatggt tttagccca 
      agacacgat tttgaatgt agcgttttc aagttagga 
      cagtttgat caaaacttt aagtatggt gctttatta 
      cccttaaga acccttttt ttgcaaaaa actaacaca 
      gttcagtgg caaaaaata tttgagcag tgtgacgta 
      gattttgtt ttaaacagg aatgatgaa atcgagtac 
      ttggtacaa aaattcttg accccactc aagaaacta 
      gaagaagaa acgcaccag ataattctt gaagacaag 
      tcggaggaa acccttggc aaaatcttc aatttctta 
      caatcgaac actcaccac aataaacaa ctgatatcg 
      ttttaccca aatgcaagt cttctaaag cacagttgt 
      acccctaac actattttc actactgaa aattttgaa 
      ataacatta agggcaatt agtggcgtt tcggaaggt 
      actccactg acttgctct tacaccgat ctttttagt 
      ggtactcat gttcgtcta aagaacctc aaaaaactc 
      aacaagact tgtttttgt gccagatgt accgaccca 
      accgaattt ggtactttt ttcagcgcc ttgaaatgc 
      atgggtacc aagcaagac ccttgtggt ggtaaccaa 
      cttccggtg aatcccaca aacgaggag actctatgg 
      gtctgtgac aagtgcaaa attgagcta ccccacaaa 
      cagatcgtc aaattcgta aaacatctc ggagggcaa 
      gttgggaaa actctcgcg aggaaagtt tcaattgaa 
      gaattagag gagttttta ggcaaattg ttaatgttt 
      ttgcacccc aaccacttt tacattttc gcggtgaaa 
      aacgctttg gttgagcta tatggtgag gagtcccta 
      gctacgaaa atccagatt tgcgaagaa ctgatcgac 
      attgctggg aaatttgaa gactggggg ttacgtctt 
      ggccgactg ttgcacgaa ttgtttgcg tccaaaacc 
      tccaatttg gttgaaaac aaaaatgtt gaagaattg 
      gcgacaaca attgcgaaa atgcgaaaa atcgcacaa 
      gtgcaccaa gacccggat tttctccaa atttttcta 
      gaagacgag tcaaaactc gcgtctttg taa
    mRNA sequence
    • >TC005637 RA
      atgattctc taagttcca cctggtttt gatgtctaa 
      aattcctga aaataattc cgtaaagtc tgaacaacc 
      aatctcgca aacgtgttg tccaggttg tctttgcaa 
      ttctgaggt aaaatatga ggtgtgcca aacaactaa 
      accccattc gattaaatc ggcaagaaa tttgtccac 
      cggagaagt aatttttag ggagttacc gctcatttc 
      tggacctcg acttgacca caaaaatga cgaaaagct 
      ctgcattgg ctgttgcaa acttctctc taatgaaaa 
      taaaattgt ttgccctaa ttgttcctt gcctttgtg 
      tagtcttga ctgtgatgg ttttagccc aagacacga 
      ttttgaatg tagcgtttt caagttagg acagtttga 
      tcaaaactt taagtatgg tgctttatt acccttaag 
      aaccctttt tttgcaaaa aactaacac agttcagtg 
      gcaaaaaat atttgagca gtgtgacgt agattttgt 
      tttaaacag gaatgatga aatcgagta cttggtaca 
      aaaattctt gaccccact caagaaact agaagaaga 
      aacgcacca gataattct tgaagacaa gtcggagga 
      aacccttgg caaaatctt caatttctt acaatcgaa 
      cactcacca caataaaca actgatatc gttttaccc 
      aaatgcaag tcttctaaa gcacagttg tacccctaa 
      cactatttt cactactga aaattttga aataacatt 
      aagggcaat tagtggcgt ttcggaagg tactccact 
      gacttgctc ttacaccga tctttttag tggtactca 
      tgttcgtct aaagaacct caaaaaact caacaagac 
      ttgtttttg tgccagatg taccgaccc aaccgaatt 
      tggtacttt tttcagcgc cttgaaatg catgggtac 
      caagcaaga cccttgtgg tggtaacca acttccggt 
      gaatcccac aaacgagga gactctatg ggtctgtga 
      caagtgcaa aattgagct accccacaa acagatcgt 
      caaattcgt aaaacatct cggagggca agttgggaa 
      aactctcgc gaggaaagt ttcaattga agaattaga 
      ggagttttt aggcaaatt gttaatgtt tttgcaccc 
      caaccactt ttacatttt cgcggtgaa aaacgcttt 
      ggttgagct atatggtga ggagtccct agctacgaa 
      aatccagat ttgcgaaga actgatcga cattgctgg 
      gaaatttga agactgggg gttacgtct tggccgact 
      gttgcacga attgtttgc gtccaaaac ctccaattt 
      ggttgaaaa caaaaatgt tgaagaatt ggcgacaac 
      aattgcgaa aatgcgaaa aatcgcaca agtgcacca 
      agacccgga ttttctcca aatttttct agaagacga 
      gtcaaaact cgcgtcttt gtaaccaca cactaataa 
      actctgcaa catttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY