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Gene information
General information
TC006447 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 9736742..9738656
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC006447
      iBeetle screen iB_09366

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC006447 RA
        MPNNIEINQ LFCFKRFPE NELKILCLV LYLPVGIVL 
        FILRTILAL SVVLIGCSL SDTRIIHKL VNKIACLTL 
        GITVTVENP DKKENVEVY ISNNLSIFD HLAVHTATG 
        SVSPGAQMH PALARGLGV HYFGTQTNL ESFKKNVAD 
        FLSEQQIPV HFTPEEKIT NGKALLKFK SYPFSLTNK 
        VQPVCISIE RPFLDLSVT TLRSSYWTD VLFYMFSPL 
        TNYKLKFLP PLEKKSVAE AEFSEIVRQ NIASALKVE 
        TPELTAADL LEWEKRQLV EQQRAPPVP RPSVDSEVR 
        RMATQVKDV LPHVPLNAI YKDLNKTRN VDTTITNIL 
        EGRVHFVPE QISSTSNNP QPSTSVASG TKASPSTST 
        TSDRDLFNT AASSFARSA HERNKSFQE RKEQLIANA 
        RRRYIEKHD LKIPF
      CDS sequence
      • >TC006447 RA
        atgccaaat aatatcgaa atcaatcag cttttttgt 
        ttcaaaagg tttcccgaa aatgagttg aagattttg 
        tgtctagtg ctttactta cctgttgga atagtcctg 
        tttatttta cggacaatc ttagctttg agtgtcgtt 
        ttgatcgga tgtagttta tcagacact cggattatc 
        cataaactt gtgaataaa attgcatgt ttaacgtta 
        ggtataaca gtcacggta gaaaatccc gacaaaaaa 
        gaaaatgta gaagtgtat attagcaat aacttatcg 
        atttttgac catttggct gtccatacg gctacagga 
        tcggtctca cccggcgct caaatgcac ccagccctg 
        gctagaggc ttaggagtt cactatttc ggtactcaa 
        acgaattta gaaagtttt aagaaaaac gtagccgac 
        tttttatcc gagcaacaa atcccagta catttcaca 
        ccggaagaa aaaatcaca aatggcaaa gcactccta 
        aaatttaaa agctaccca ttctccctg acaaataaa 
        gtgcaacct gtttgtatc agcattgaa cgcccgttt 
        ctggacctg tctgttaca actctacgc tcatcttat 
        tggactgac gttttattt tacatgttt tcgcctctt 
        actaactat aaattgaaa tttttgcct cctcttgag 
        aaaaagtca gtggcggag gctgaattt tccgaaatc 
        gtacgccag aatatcgcg tcggccctt aaggtggaa 
        acccccgaa ttgacagct gccgatctc ttagagtgg 
        gaaaaacgc caacttgtt gaacaacag agagcgcca 
        cctgtgcct aggccaagc gttgatagt gaagtcagg 
        agaatggcg acacaagtt aaagatgtg ctaccacat 
        gtgccatta aacgcaatt tacaaggac ttgaacaaa 
        acacgaaat gtagacaca actataacg aatatcctc 
        gaaggccga gttcacttt gtgccagaa caaattagt 
        tctacctcc aacaatcca caaccttcc acatctgta 
        gcatctggt acgaaagca tcgccttcg acttcaact 
        acaagtgat agggatttg ttcaataca gctgcttcg 
        tcttttgcc cgatctgcc cacgagaga aacaagtca 
        tttcaggag agaaaagaa caacttatt gccaatgca 
        aggaggcga tatattgaa aaacacgac ttaaagata 
        ccgttttag
      mRNA sequence
      • >TC006447 RA
        cgtcacaaa cctaaccct ctgttatgt tatttagtg 
        taaaacctt aagataatt ttgtttttg ttatgtgaa 
        aatgccaaa taatatcga aatcaatca gcttttttg 
        tttcaaaag gtttcccga aaatgagtt gaagatttt 
        gtgtctagt gctttactt acctgttgg aatagtcct 
        gtttatttt acggacaat cttagcttt gagtgtcgt 
        tttgatcgg atgtagttt atcagacac tcggattat 
        ccataaact tgtgaataa aattgcatg tttaacgtt 
        aggtataac agtcacggt agaaaatcc cgacaaaaa 
        agaaaatgt agaagtgta tattagcaa taacttatc 
        gatttttga ccatttggc tgtccatac ggctacagg 
        atcggtctc acccggcgc tcaaatgca cccagccct 
        ggctagagg cttaggagt tcactattt cggtactca 
        aacgaattt agaaagttt taagaaaaa cgtagccga 
        ctttttatc cgagcaaca aatcccagt acatttcac 
        accggaaga aaaaatcac aaatggcaa agcactcct 
        aaaatttaa aagctaccc attctccct gacaaataa 
        agtgcaacc tgtttgtat cagcattga acgcccgtt 
        tctggacct gtctgttac aactctacg ctcatctta 
        ttggactga cgttttatt ttacatgtt ttcgcctct 
        tactaacta taaattgaa atttttgcc tcctcttga 
        gaaaaagtc agtggcgga ggctgaatt ttccgaaat 
        cgtacgcca gaatatcgc gtcggccct taaggtgga 
        aacccccga attgacagc tgccgatct cttagagtg 
        ggaaaaacg ccaacttgt tgaacaaca gagagcgcc 
        acctgtgcc taggccaag cgttgatag tgaagtcag 
        gagaatggc gacacaagt taaagatgt gctaccaca 
        tgtgccatt aaacgcaat ttacaagga cttgaacaa 
        aacacgaaa tgtagacac aactataac gaatatcct 
        cgaaggccg agttcactt tgtgccaga acaaattag 
        ttctacctc caacaatcc acaaccttc cacatctgt 
        agcatctgg tacgaaagc atcgccttc gacttcaac 
        tacaagtga tagggattt gttcaatac agctgcttc 
        gtcttttgc ccgatctgc ccacgagag aaacaagtc 
        atttcagga gagaaaaga acaacttat tgccaatgc 
        aaggaggcg atatattga aaaacacga cttaaagat 
        accgtttta ggatgtttg tttgttttt gataagttt 
        ttttttttt tagttaagt tgtggagtt tgaggcaca 
        ttttcagaa ttttggttt aagtttaag tttggcgat 
        tctttgcgg tttttacta cttaaactg taaatattg 
        ttatttatt tttggttgc aatacaata aacatttca 
        ttacacg
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY