Status
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Gene information
General information
TC015223 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7028432..7040706
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC015223
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09364

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC015223 RA
      MIGRRLRSA IPPTWKSNK FRLIFIPVV CVKKLGYLA 
      CRTVMPSEK LERVVLKRI NKMEGDITQ NNSRAELVR 
      SSEESNETS HSERTIKDV KFREWVMVF ILCFVNLIN 
      YMDRFTLAG ILEDIQKYF NVQNDKGGL LQTAFVLSY 
      MIFAPIFGY LGDRYSRKN IMAFGVFLW SLTTLIGSF 
      MTDFWFFLL FRALVGIGE ASYSTIAPT IISDLFVGD 
      VRSKMLALF YFAIPVGSG MGYIVGSET AKAFGKWQW 
      GLRVTPLLG VVAVVLIFF VLRDPERGQ SEGSSHIQT 
      TPWTEDLKD LVKNKSFMI STAGFTCVT FVAGALAWW 
      GPKFIEMGL SLQPHASSL DSQSVSYKF GLVSMVSGI 
      LGVPAGSLV AQHLRHRYP RIDAHLCGI ALLLSTPMV 
      FAACLTASY SLSLCFTFV FFGELFLNL TWSIVADIL 
      LYVVLPTRR STAEGFQLL VSHALGDAG SPYLIGVIS 
      EAVLIMLNN SSKSKGNDF KALQYALFT TCFVEILGA 
      FFFLVNALY ILQDKHKVD KAIQANSQQ LLTDGSETE 
      NPS
    CDS sequence
    • >TC015223 RA
      atgatcggt cggcgcctg cgcagtgca atcccgcca 
      acatggaaa tcaaacaag tttcgtttg attttcatt 
      ccggtcgta tgtgtcaag aagttaggt tatttagcg 
      tgtcgtact gtcatgccg tctgaaaag ttagaacga 
      gtagtgctg aaaagaatt aataaaatg gaaggtgac 
      ataacgcag aataactca agagcagaa cttgtacgt 
      tccagtgag gaaagcaac gaaacctcg cacagtgaa 
      aggactatc aaagatgtt aaatttcgg gaatgggtt 
      atggttttt atattatgt ttcgtcaac cttatcaac 
      tacatggat aggttcacc ttagcaggc attttggaa 
      gacattcag aaatatttc aacgtgcag aatgacaaa 
      ggcgggctt ttgcagacg gccttcgtc ctgagttac 
      atgattttt gcccccatt ttcgggtac ttgggggac 
      aggtacagc aggaaaaat atcatggcg ttcggggtc 
      ttcctttgg agtcttacc accctgatt ggctccttc 
      atgacggat ttttggttc ttcctgttg ttcagggct 
      ttggtagga ataggtgaa gccagctat tccaccatt 
      gcgccgacg attatcagc gatttgttc gtcggtgat 
      gtcaggtcg aaaatgctg gcgcttttt tacttcgcc 
      attccggta ggaagcggt atgggttat atcgtgggt 
      tccgaaacg gccaaagcc ttcgggaag tggcagtgg 
      gggctccgt gtgacccca cttctgggg gttgtggcc 
      gtagtccta atcttcttt gtgcttcgc gaccccgaa 
      aggggtcaa agtgagggc agtagccac atccagacc 
      accccatgg actgaagat ttgaaggat ttggtcaaa 
      aataaaagt ttcatgatc tcgactgcg ggcttcacg 
      tgtgtcact ttcgtggcc ggagcgttg gcctggtgg 
      gggcctaaa tttatcgaa atggggctc agtttacag 
      ccgcatgcg agcagcttg gatagtcag agcgtttcg 
      tataaattc ggcttggtg tcgatggtg tcgggcatt 
      ctgggcgtc ccagcggga agtctcgtg gcgcaacat 
      ttgcgccac cgttacccc cgtatcgac gcccatttg 
      tgcggtatt gccctttta ttgtcgact cctatggtg 
      ttcgccgct tgcctcacc gcctcgtac agcctcagt 
      ctttgtttc actttcgta tttttcggt gaactcttc 
      ctaaacctc acctggtcg atcgtggcc gacattctg 
      ctgtacgtt gtgttgcct acgcgaagg tcgaccgct 
      gagggcttc caacttctg gtgtcacac gcgctgggg 
      gatgccgga agtccttac ctgatcgga gttatttcc 
      gaagccgtt ttaataatg ctcaacaat tcgtcaaag 
      tccaaaggg aatgatttt aaggcgctt cagtacgct 
      ttattcacg acgtgtttc gtcgaaatc ctgggagca 
      ttcttcttc ctcgtcaat gccttgtac attttgcaa 
      gataagcac aaagtcgac aaagcaatt caggcaaac 
      tcccaacaa ctcctaacg gatggttca gaaacggaa 
      aatccttcc tga
    mRNA sequence
    • >TC015223 RA
      aaaacagcg atctgtaat tggcgactt ttcaacagt 
      tgggttcgg gcagctgtc accgttttg cgacttttg 
      ttgcaaact ttgcgtgtt tttgtgcat gttttgtgt 
      ccgagttag tgtttttgt ccgtgattg ttatcctta 
      agtgttttt accgcgtgc ccccttaaa aagcccttt 
      tggttggga aggatatcg tatgatcgg tcggcgcct 
      gcgcagtgc aatcccgcc aacatggaa atcaaacaa 
      gtttcgttt gattttcat tccggtcgt atgtgtcaa 
      gaagttagg ttatttagc gtgtcgtac tgtcatgcc 
      gtctgaaaa gttagaacg agtagtgct gaaaagaat 
      taataaaat ggaaggtga cataacgca gaataactc 
      aagagcaga acttgtacg ttccagtga ggaaagcaa 
      cgaaacctc gcacagtga aaggactat caaagatgt 
      taaatttcg ggaatgggt tatggtttt tatattatg 
      tttcgtcaa ccttatcaa ctacatgga taggttcac 
      cttagcagg cattttgga agacattca gaaatattt 
      caacgtgca gaatgacaa aggcgggct tttgcagac 
      ggccttcgt cctgagtta catgatttt tgcccccat 
      tttcgggta cttggggga caggtacag caggaaaaa 
      tatcatggc gttcggggt cttcctttg gagtcttac 
      caccctgat tggctcctt catgacgga tttttggtt 
      cttcctgtt gttcagggc tttggtagg aataggtga 
      agccagcta ttccaccat tgcgccgac gattatcag 
      cgatttgtt cgtcggtga tgtcaggtc gaaaatgct 
      ggcgctttt ttacttcgc cattccggt aggaagcgg 
      tatgggtta tatcgtggg ttccgaaac ggccaaagc 
      cttcgggaa gtggcagtg ggggctccg tgtgacccc 
      acttctggg ggttgtggc cgtagtcct aatcttctt 
      tgtgcttcg cgaccccga aaggggtca aagtgaggg 
      cagtagcca catccagac caccccatg gactgaaga 
      tttgaagga tttggtcaa aaataaaag tttcatgat 
      ctcgactgc gggcttcac gtgtgtcac tttcgtggc 
      cggagcgtt ggcctggtg ggggcctaa atttatcga 
      aatggggct cagtttaca gccgcatgc gagcagctt 
      ggatagtca gagcgtttc gtataaatt cggcttggt 
      gtcgatggt gtcgggcat tctgggcgt cccagcggg 
      aagtctcgt ggcgcaaca tttgcgcca ccgttaccc 
      ccgtatcga cgcccattt gtgcggtat tgccctttt 
      attgtcgac tcctatggt gttcgccgc ttgcctcac 
      cgcctcgta cagcctcag tctttgttt cactttcgt 
      atttttcgg tgaactctt cctaaacct cacctggtc 
      gatcgtggc cgacattct gctgtacgt tgtgttgcc 
      tacgcgaag gtcgaccgc tgagggctt ccaacttct 
      ggtgtcaca cgcgctggg ggatgccgg aagtcctta 
      cctgatcgg agttatttc cgaagccgt tttaataat 
      gctcaacaa ttcgtcaaa gtccaaagg gaatgattt 
      taaggcgct tcagtacgc tttattcac gacgtgttt 
      cgtcgaaat cctgggagc attcttctt cctcgtcaa 
      tgccttgta cattttgca agataagca caaagtcga 
      caaagcaat tcaggcaaa ctcccaaca actcctaac 
      ggatggttc agaaacgga aaatccttc ctgattatt 
      ggtgtatta agcgagtga attagactt tcgtagcgc 
      gtgtgattt gaacgtgta aggtttttc gtgtaaata 
      tgagtttgt gagagtact aattgtgtt ttgtacaag 
      actgatttt catttgcgt tttgtcaaa cgtaaagat 
      caaatacgc acacgattt cgtattata ttatgatct 
      tgtgatcat tcagtacgt tttttagaa tggtattag 
      ttattgttt tatttatta caaatttgt tatcacata 
      tagcgaaat aaaaattaa taagccac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY