Status
Please wait ...
Gene information
General information
TC006411 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 8505700..8507478
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006411
    iBeetle screen iB_09359

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006411 RA
      MATSKTTNT YNRQNWEDA DFPILCQTC LGDNPYIRM 
      TKEKYGKEC KICARPFTV FRWCPGARM RFKKTEVCQ 
      TCSKLKNVC QTCLLDLEY GLPIQVRDA ALKLKDDLP 
      KSDVNKEYY IQNMERELQ RSDTGVLSN KLNEPNDLL 
      MRLARTAPY YKRNRPHVC SFWVKGECR RGEECPYRH 
      EKPTDPEDP LADQNIKDR YYGVNDPVA DKLLKRAAA 
      MPALPPPED KTITTLYIG NLGTLTEQD IRDHFYQYG 
      EIRSVSLVP KQQCAFVQY TTRAAAETA AEKTFNKLI 
      LGGRRLTIK WGRSQGRAG PAINPGLEA YEPVPGLPG 
      ALPELSNNF FNLEPGHIP LPNMPPPPS LIVPPMFGP 
      PPMPPFFFN PPQLSAYVP TAASSSTVT SEATGGPSK 
      STVHYPSQD PARLGATQL HADRQE
    CDS sequence
    • >TC006411 RA
      atggcaacg tcaaaaaca acaaacaca tacaatcgg 
      caaaactgg gaggatgcg gacttcccg attttgtgt 
      caaacctgt ctgggggac aacccttat attcgcatg 
      actaaagaa aaatatggc aaggagtgt aaaatctgt 
      gcgcgccct ttcacagtg tttcggtgg tgtcctggg 
      gctcgtatg cgtttcaaa aagacagaa gtttgccag 
      acttgtagc aagttgaag aacgtgtgt cagacgtgt 
      ctcctggac ttggagtac ggcctccct atacaggtc 
      cgggacgct gccctcaaa ctcaaggat gatctcccc 
      aagagcgac gttaataag gaatattat atccagaat 
      atggagcgc gagttgcaa cggtcggat actggcgtt 
      ttgtcgaat aagttgaac gaaccgaat gacttgcta 
      atgcgtttg gcgcgaaca gccccctac tacaaacga 
      aacaggccc cacgtatgt tcattttgg gtgaaaggg 
      gagtgcaga agaggcgag gagtgtcct tacagacac 
      gagaagcca acagatcct gaagacccc cttgctgat 
      caaaacata aaagaccgc tactacggc gtgaacgac 
      cctgttgct gataaatta ttgaaaaga gcggcagcc 
      atgcctgca ttaccgccc cctgaagac aaaaccatc 
      acaactcta tacatcggc aatcttggt actctcacc 
      gaacaagac atcagggac cacttctac caatacggg 
      gagatccgc tctgttagt ttagtccct aaacagcaa 
      tgcgccttc gtccagtac acgaccaga gcagccgct 
      gaaacggcc gccgaaaaa accttcaat aagttgatt 
      ttgggcggc cgccgactt accatcaaa tgggggcgg 
      tctcaagga cgcgcgggc cccgcgatt aacccggga 
      cttgaggcc tatgaacct gtgccaggc cttccgggg 
      gctctgcct gaattgagt aataatttc tttaatctc 
      gaaccggga cacattcca ttgccgaat atgccgcca 
      ccgccgtct ctcatcgtt ccgccgatg ttcggcccc 
      ccgcccatg cccccattt ttctttaat ccgccccag 
      ttgtcggcg tatgtcccc acagcggct tcgagcagc 
      actgtgacg agtgaggcg acagggggg ccctccaaa 
      agcacagtg cattatccc agccaggat ccggccagg 
      ctgggagcc acgcagttg catgcagat aggcaggag 
      tag
    mRNA sequence
    • >TC006411 RA
      gttggcagc actgagtca cgtcaagta actatcaat 
      aaagcttgt ttttagttt tcgtataat ttgagcaaa 
      acccgagca taaatggtg tgaaactgc aatggcaac 
      gtcaaaaac aacaaacac atacaatcg gcaaaactg 
      ggaggatgc ggacttccc gattttgtg tcaaacctg 
      tctggggga caaccctta tattcgcat gactaaaga 
      aaaatatgg caaggagtg taaaatctg tgcgcgccc 
      tttcacagt gtttcggtg gtgtcctgg ggctcgtat 
      gcgtttcaa aaagacaga agtttgcca gacttgtag 
      caagttgaa gaacgtgtg tcagacgtg tctcctgga 
      cttggagta cggcctccc tatacaggt ccgggacgc 
      tgccctcaa actcaagga tgatctccc caagagcga 
      cgttaataa ggaatatta tatccagaa tatggagcg 
      cgagttgca acggtcgga tactggcgt tttgtcgaa 
      taagttgaa cgaaccgaa tgacttgct aatgcgttt 
      ggcgcgaac agcccccta ctacaaacg aaacaggcc 
      ccacgtatg ttcattttg ggtgaaagg ggagtgcag 
      aagaggcga ggagtgtcc ttacagaca cgagaagcc 
      aacagatcc tgaagaccc ccttgctga tcaaaacat 
      aaaagaccg ctactacgg cgtgaacga ccctgttgc 
      tgataaatt attgaaaag agcggcagc catgcctgc 
      attaccgcc ccctgaaga caaaaccat cacaactct 
      atacatcgg caatcttgg tactctcac cgaacaaga 
      catcaggga ccacttcta ccaatacgg ggagatccg 
      ctctgttag tttagtccc taaacagca atgcgcctt 
      cgtccagta cacgaccag agcagccgc tgaaacggc 
      cgccgaaaa aaccttcaa taagttgat tttgggcgg 
      ccgccgact taccatcaa atgggggcg gtctcaagg 
      acgcgcggg ccccgcgat taacccggg acttgaggc 
      ctatgaacc tgtgccagg ccttccggg ggctctgcc 
      tgaattgag taataattt ctttaatct cgaaccggg 
      acacattcc attgccgaa tatgccgcc accgccgtc 
      tctcatcgt tccgccgat gttcggccc cccgcccat 
      gcccccatt tttctttaa tccgcccca gttgtcggc 
      gtatgtccc cacagcggc ttcgagcag cactgtgac 
      gagtgaggc gacaggggg gccctccaa aagcacagt 
      gcattatcc cagccagga tccggccag gctgggagc 
      cacgcagtt gcatgcaga taggcagga gtaggtctt 
      aggtcttat gaggtgtac aatttttta tcaataaaa 
      aattatgac cagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY