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Gene information
General information
TC005793 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7533410..7537130
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005793
    iBeetle screen iB_09351

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005793 RA
      MHPYVRRTI RATLASLEW LRITTLRVT TRIIELIVL 
      SWLSVYYIA EALILTFTP AFLRRQKTL RGKIVLVTG 
      GAGGVGQEL ALRLARQKA RVVIWDNNE KALEKVREK 
      IESEGYKVH TYPVDVTDR ENVYKYADI VKSDIGHID 
      VLINNAGIV CGQTFLEIP DYMIEKTFK VNILSHYWT 
      TKAFLPNMI KTGKGHIVT IGSLTGLLG TYKCTDYSA 
      SKHATIGFH ESLLIELKT HGHHQIKMT LVCPYFINT 
      GMFAGCKPR NMNMLEPKD VAKRIITAI RREEVYVTM 
      PAFSRFVLP VKNFIPAKL NWALVYKVI QGPQSMMGM 
      RNFQEVEAA
    CDS sequence
    • >TC005793 RA
      atgcatcct tacgtccgt cgaacaatc agggcgact 
      ttagcctcg ctcgagtgg ttgcgcata accaccctt 
      cgtgtaacc accagaatt atcgaactg attgttttg 
      agttggttg agtgtttat tatattgcc gaagctctg 
      atcttgacc ttcacgccg gcgtttctt cgtcggcag 
      aaaaccctt cgagggaaa atcgttttg gtgacggga 
      ggtgccggt ggagtaggt caagagtta gccctgaga 
      cttgccaga caaaaggct cgagttgtc atttgggac 
      aataatgaa aaagcttta gaaaaggtg cgtgaaaaa 
      atagaatcg gaaggttac aaagttcac acttatcct 
      gtggacgtc accgatcgc gaaaacgtt tacaaatac 
      gccgacatt gtaaaatcc gacataggc catatcgac 
      gtcctcata aacaacgcc ggaatcgtt tgtggccaa 
      acattcctc gaaatcccg gattatatg atcgaaaaa 
      acgttcaaa gtgaacatt ttatcgcac tattggacc 
      actaaagcc tttctcccc aacatgatc aagacggga 
      aaaggccac attgtcaca atcggaagt ctcaccgga 
      cttttgggc acttacaag tgcaccgat tacagtgcc 
      tccaaacat gcaacgatt ggtttccac gaaagtctc 
      ctaatcgag ctcaaaacg cacggtcat catcagatt 
      aaaatgacg ttggtttgc ccgtatttt atcaacaca 
      gggatgttt gccggctgc aagcccaga aacatgaac 
      atgttggaa ccgaaggat gttgcaaaa aggataatt 
      acagccatt aggagggag gaagtctat gtcacaatg 
      ccggctttc tcgcggttt gtcctcccc gtcaaaaac 
      ttcattccg gcaaaacta aactgggcc ctcgtttac 
      aaagtaatt caagggccg caatctatg atgggaatg 
      agaaacttc caagaagta gaagctgcc taa
    mRNA sequence
    • >TC005793 RA
      agagaaagg tttgggcag gtcatcaaa ctagaattt 
      tctccaacg acctgctaa ctgagatat tgccaagga 
      aagattcac actcgcgac caaaagccg gcatttcca 
      attcaagcc gcgtcgcga gcggttacc ttatcgtga 
      tacgtgtgt gaaataaat tagtttaaa aatgcatcc 
      ttacgtccg tcgaacaat cagggcgac tttagcctc 
      gctcgagtg gttgcgcat aaccaccct tcgtgtaac 
      caccagaat tatcgaact gattgtttt gagttggtt 
      gagtgttta ttatattgc cgaagctct gatcttgac 
      cttcacgcc ggcgtttct tcgtcggca gaaaaccct 
      tcgagggaa aatcgtttt ggtgacggg aggtgccgg 
      tggagtagg tcaagagtt agccctgag acttgccag 
      acaaaaggc tcgagttgt catttggga caataatga 
      aaaagcttt agaaaaggt gcgtgaaaa aatagaatc 
      ggaaggtta caaagttca cacttatcc tgtggacgt 
      caccgatcg cgaaaacgt ttacaaata cgccgacat 
      tgtaaaatc cgacatagg ccatatcga cgtcctcat 
      aaacaacgc cggaatcgt ttgtggcca aacattcct 
      cgaaatccc ggattatat gatcgaaaa aacgttcaa 
      agtgaacat tttatcgca ctattggac cactaaagc 
      ctttctccc caacatgat caagacggg aaaaggcca 
      cattgtcac aatcggaag tctcaccgg acttttggg 
      cacttacaa gtgcaccga ttacagtgc ctccaaaca 
      tgcaacgat tggtttcca cgaaagtct cctaatcga 
      gctcaaaac gcacggtca tcatcagat taaaatgac 
      gttggtttg cccgtattt tatcaacac agggatgtt 
      tgccggctg caagcccag aaacatgaa catgttgga 
      accgaagga tgttgcaaa aaggataat tacagccat 
      taggaggga ggaagtcta tgtcacaat gccggcttt 
      ctcgcggtt tgtcctccc cgtcaaaaa cttcattcc 
      ggcaaaact aaactgggc cctcgttta caaagtaat 
      tcaagggcc gcaatctat gatgggaat gagaaactt 
      ccaagaagt agaagctgc ctaagccca catttcaaa 
      taaattaga ggtgtttgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY