Status
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Gene information
General information
TC005802 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7442547..7446324
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005802
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09348

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005802 RA
      MSPLINGFI IFSLTVTLL YFVGLLTFV NDHEENKCE 
      MTYMFEYPQ FVRISNKID DKYPRYGLY AYSEGRLTE 
      KTRNMFFDG IPVLFIPGN AGSHRQGRS LASVALRKA 
      LNSRTPFHF DYFLIDLNN ELSGLNGAL LADQLEYVN 
      NSIYTVLDL YQNRKNPAK SVVLVGHSM GGIIAKRLV 
      SNLGENSDL VPIVLGLAT PHRRPPLQL DSFSYDFYK 
      NIDTKNSST TFVSITGGY NDFLVPSFL TNHPDSILN 
      VVSTQIPRS WLPADHVCI LWCKQSILA INRALVDSV 
      DPKTKQISS DQTLRYRVF HHHLIHHSG INIRLLDSY 
      SLSPNIKNQ GEWIEPFGR QYSVQVPRL SSSPHFYMI 
      RLLNQPQHQ TLTVVALNL ETVDWAFVC GAHIPRGNS 
      RICEEFTHL THLSEISPS SNYKRRSLT VDMHELMKN 
      NTVWTHIVL RVLPTDEQV ILNVDVYNT GDRKTLVNL 
      PNLLSFKRQ TIIEQTHEK AIHYELVLP NLYHLMQSY 
      QLYVESIQC VNQFRHATA SLIVPWGHQ NQHKFFIDG 
      DNKPMNVRL YSSRPQWAS NQSAVIQLR LDPTCRYKI 
      SIQSSFIGV LSQLARFYS PLLIVNVAA VILVALNTQ 
      LKSLGQEND CCLFFTAVK EGAKPYYIL TSVKLISRF 
      LSFKPFVTY LAAPDWLIM SEEGTDFFL LPLLLYICS 
      VGIVWLLGI FLSVSLVVC ETTVHKFTL KFLAKTVSF 
      TVSWSDYLM SALHKLPCI VATFLICLC VTTTGSLAL 
      CVGMVFYFM RLTQLSQDY IEELVWYYV KKIGKKLKK 
      PNKDKDNNS INSGETPAH NAIFFHATL FLLWSILTV 
      INVPSVLTW AHNFKFSTA LKPDPAFYP GLVLSICAL 
      PLWQLDVPK IERRGYVEL GKLIIVLAV ASLIYAPIS 
      LFRLNFTLT SAIAFVTLH QLVAPNREI PETSRTEQP 
      ISYETIKAK LE
    CDS sequence
    • >TC005802 RA
      atgtcaccg ctaataaat ggttttatt atattttcg 
      ttgactgta acgctactc tatttcgtc ggtttgctg 
      acttttgtc aacgaccat gaagagaac aagtgtgag 
      atgacctac atgttcgag tacccccaa tttgtgcga 
      atatcaaac aaaatcgac gataaatac cccagatat 
      ggactttac gcttacagt gaaggtcgc ctcaccgaa 
      aaaacccga aacatgttt ttcgacggc atccctgta 
      ttatttata ccgggaaat gccggctcg catcgtcaa 
      ggacgctct ctagcatcg gtggccctt cgtaaagct 
      ttaaactcg cgaacacct tttcacttt gactatttc 
      ctaatcgat ttaaataat gaactcagc ggcctaaac 
      ggggcactt ctcgccgat caactcgaa tacgtcaac 
      aattcgatt tacaccgtt ttagattta taccaaaat 
      cgaaaaaac ccagcaaag tcggtggtc ctagttggc 
      cattctatg ggtggtatc attgccaaa cggttggta 
      tcaaacttg ggggaaaat tccgacttg gtgccaatc 
      gtccttggc ctagctaca ccacaccga aggccccca 
      ctgcaactt gattcattt tcgtacgat ttttacaaa 
      aatattgac actaaaaat agctccacc acatttgta 
      agcatcaca gggggttat aatgatttt ctagtgcct 
      tcgtttctc accaatcac cctgatagt atcttaaat 
      gtcgtgagt actcaaatc ccacggtcg tggctccca 
      gccgaccac gtttgcatc ctttggtgc aaacaatcg 
      attttagct ataaacaga gctctcgtt gattcagtc 
      gatccgaaa acaaaacaa atatcaagc gatcaaact 
      ctgcgttat agggttttt caccaccat ttgattcac 
      cacagcggt attaatatc cgacttttg gatagttat 
      agtctcagt ccgaatatt aagaatcaa ggggagtgg 
      attgagccc ttcgggcgc cagtactcg gtccaagtg 
      ccccgtctg tcaagcagc ccccatttt tacatgatt 
      aggcttttg aatcaaccc caacaccaa accctaact 
      gtggtagcc ctgaatttg gaaactgta gattgggct 
      ttcgtatgt ggggctcac ataccacgg gggaactcc 
      cggatttgc gaagagttc actcatttg acacacttg 
      tcagaaatt tcgccctca agtaattac aaaagacgt 
      tcactaact gtagatatg cacgaattg atgaaaaac 
      aacacagtg tggacacac atcgtcctg cgagtgcta 
      ccaaccgac gaacaagta attctaaac gttgatgtt 
      tataacact ggtgacaga aaaacccta gtcaattta 
      cccaactta ctatcattc aaacgccaa acaatcatc 
      gaacaaaca cacgaaaaa gcaatacac tatgagttg 
      gtactaccg aatttgtat cacttgatg cagtcgtac 
      caactgtac gtcgaatcg attcaatgc gtcaatcaa 
      ttcagacac gccacagct agcttgatt gtaccatgg 
      ggacaccaa aaccaacac aaatttttc atcgacgga 
      gataacaaa ccgatgaac gttagactg tatagttca 
      aggccacaa tgggcctca aaccagtcg gctgtgatc 
      caactaaga cttgaccca acttgtcgc tacaaaatc 
      agcatccag tcatcattt attggcgtt ttatcgcag 
      ttggcacga ttttattca ccactgttg attgttaat 
      gtggcagca gtgattttg gtggcctta aatacgcaa 
      ttaaaatcc ctaggacag gaaaacgac tgttgctta 
      tttttcaca gcggtgaaa gaaggagca aaaccgtat 
      tatattttg acaagtgtc aaattaatt tcgcgtttt 
      ttgagtttc aaaccattc gtcacgtat ttggcggcc 
      cccgattgg ttgataatg tcagaggag gggacagat 
      tttttcttg ctgcccctc ctactttac atttgtagc 
      gttggtatt gtgtggctt ttggggatt tttttgtct 
      gtctcattg gtagtatgt gagacgaca gtacataag 
      tttacccta aagtttttg gcaaaaact gtcagtttt 
      actgtcagt tggtcggat tatttaatg tcagctctg 
      cacaaactg ccttgcatt gttgccacc tttctcata 
      tgtctttgt gtgacaacg accggtagt ctcgcactt 
      tgtgtcgga atggttttt tattttatg aggctgaca 
      caactatca caagattac attgaggag ttagtgtgg 
      tattatgtg aaaaaaatc ggcaaaaaa cttaaaaaa 
      ccgaacaaa gataaagat aataatagt attaatagt 
      ggggagaca ccagctcac aacgctata tttttccac 
      gccacttta tttcttctg tggtccata ctaacagtc 
      ataaacgtg ccatccgtg ctaacgtgg gcccacaat 
      ttcaaattt agcaccgcc ctcaaacca gaccccgcc 
      ttttatccc gggctggtc ctaagcatc tgcgctttg 
      cctctgtgg caattggac gttccaaaa atcgaaagg 
      cggggctat gtcgagcta ggcaaattg ataatagtt 
      ctggccgtc gccagcttg atttacgcg ccgatttca 
      ctttttaga ttaaatttc acattgact agtgcaatt 
      gcgtttgtt actttgcat cagttggta gcgccgaat 
      cgagaaata cccgaaacg agccgaacg gaacaaccg 
      atttcttac gagacgatc aaggcgaaa cttgaatag
    mRNA sequence
    • >TC005802 RA
      ccccgagtt atgtcaccg ctaataaat ggttttatt 
      atattttcg ttgactgta acgctactc tatttcgtc 
      ggtttgctg acttttgtc aacgaccat gaagagaac 
      aagtgtgag atgacctac atgttcgag tacccccaa 
      tttgtgcga atatcaaac aaaatcgac gataaatac 
      cccagatat ggactttac gcttacagt gaaggtcgc 
      ctcaccgaa aaaacccga aacatgttt ttcgacggc 
      atccctgta ttatttata ccgggaaat gccggctcg 
      catcgtcaa ggacgctct ctagcatcg gtggccctt 
      cgtaaagct ttaaactcg cgaacacct tttcacttt 
      gactatttc ctaatcgat ttaaataat gaactcagc 
      ggcctaaac ggggcactt ctcgccgat caactcgaa 
      tacgtcaac aattcgatt tacaccgtt ttagattta 
      taccaaaat cgaaaaaac ccagcaaag tcggtggtc 
      ctagttggc cattctatg ggtggtatc attgccaaa 
      cggttggta tcaaacttg ggggaaaat tccgacttg 
      gtgccaatc gtccttggc ctagctaca ccacaccga 
      aggccccca ctgcaactt gattcattt tcgtacgat 
      ttttacaaa aatattgac actaaaaat agctccacc 
      acatttgta agcatcaca gggggttat aatgatttt 
      ctagtgcct tcgtttctc accaatcac cctgatagt 
      atcttaaat gtcgtgagt actcaaatc ccacggtcg 
      tggctccca gccgaccac gtttgcatc ctttggtgc 
      aaacaatcg attttagct ataaacaga gctctcgtt 
      gattcagtc gatccgaaa acaaaacaa atatcaagc 
      gatcaaact ctgcgttat agggttttt caccaccat 
      ttgattcac cacagcggt attaatatc cgacttttg 
      gatagttat agtctcagt ccgaatatt aagaatcaa 
      ggggagtgg attgagccc ttcgggcgc cagtactcg 
      gtccaagtg ccccgtctg tcaagcagc ccccatttt 
      tacatgatt aggcttttg aatcaaccc caacaccaa 
      accctaact gtggtagcc ctgaatttg gaaactgta 
      gattgggct ttcgtatgt ggggctcac ataccacgg 
      gggaactcc cggatttgc gaagagttc actcatttg 
      acacacttg tcagaaatt tcgccctca agtaattac 
      aaaagacgt tcactaact gtagatatg cacgaattg 
      atgaaaaac aacacagtg tggacacac atcgtcctg 
      cgagtgcta ccaaccgac gaacaagta attctaaac 
      gttgatgtt tataacact ggtgacaga aaaacccta 
      gtcaattta cccaactta ctatcattc aaacgccaa 
      acaatcatc gaacaaaca cacgaaaaa gcaatacac 
      tatgagttg gtactaccg aatttgtat cacttgatg 
      cagtcgtac caactgtac gtcgaatcg attcaatgc 
      gtcaatcaa ttcagacac gccacagct agcttgatt 
      gtaccatgg ggacaccaa aaccaacac aaatttttc 
      atcgacgga gataacaaa ccgatgaac gttagactg 
      tatagttca aggccacaa tgggcctca aaccagtcg 
      gctgtgatc caactaaga cttgaccca acttgtcgc 
      tacaaaatc agcatccag tcatcattt attggcgtt 
      ttatcgcag ttggcacga ttttattca ccactgttg 
      attgttaat gtggcagca gtgattttg gtggcctta 
      aatacgcaa ttaaaatcc ctaggacag gaaaacgac 
      tgttgctta tttttcaca gcggtgaaa gaaggagca 
      aaaccgtat tatattttg acaagtgtc aaattaatt 
      tcgcgtttt ttgagtttc aaaccattc gtcacgtat 
      ttggcggcc cccgattgg ttgataatg tcagaggag 
      gggacagat tttttcttg ctgcccctc ctactttac 
      atttgtagc gttggtatt gtgtggctt ttggggatt 
      tttttgtct gtctcattg gtagtatgt gagacgaca 
      gtacataag tttacccta aagtttttg gcaaaaact 
      gtcagtttt actgtcagt tggtcggat tatttaatg 
      tcagctctg cacaaactg ccttgcatt gttgccacc 
      tttctcata tgtctttgt gtgacaacg accggtagt 
      ctcgcactt tgtgtcgga atggttttt tattttatg 
      aggctgaca caactatca caagattac attgaggag 
      ttagtgtgg tattatgtg aaaaaaatc ggcaaaaaa 
      cttaaaaaa ccgaacaaa gataaagat aataatagt 
      attaatagt ggggagaca ccagctcac aacgctata 
      tttttccac gccacttta tttcttctg tggtccata 
      ctaacagtc ataaacgtg ccatccgtg ctaacgtgg 
      gcccacaat ttcaaattt agcaccgcc ctcaaacca 
      gaccccgcc ttttatccc gggctggtc ctaagcatc 
      tgcgctttg cctctgtgg caattggac gttccaaaa 
      atcgaaagg cggggctat gtcgagcta ggcaaattg 
      ataatagtt ctggccgtc gccagcttg atttacgcg 
      ccgatttca ctttttaga ttaaatttc acattgact 
      agtgcaatt gcgtttgtt actttgcat cagttggta 
      gcgccgaat cgagaaata cccgaaacg agccgaacg 
      gaacaaccg atttcttac gagacgatc aaggcgaaa 
      cttgaatag gttccagtc aaggctgtt tttatttgt 
      gatctctgc tatttgatt taattcaaa tttcccgct 
      cttgtccag gatcaagat ggctaaact ggactccac 
      aggaatgag aattgtgat tttcccgaa caatttgtt 
      tatttacgc tttgttaga cacgattag caagcaaat 
      gtttaattt taagcttta atgcggcag tatttcacc 
      tgtttatcg ttaagtgca aataaacca ataatgacg 
      ttattttgt cgttttctt tcaattttc ttaccttaa 
      cacgtccct tccttgctc cggaaatac acttcgggc 
      acaaagcat ggtttttat cacagtttt atcacaaaa 
      acacgacac aacaccgac ttgtcactc gcgccttta 
      ctataac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY