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Gene information
General information
TC030327 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14362941..14364382
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030327
    iBeetle screen iB_09342

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030327 RA
      MENPLKLLH IIGLDPRQS DKYSTIKKV ISFLIVLAV 
      LLSALIEFF LHHNESQVY DTAPQSTVP NLQALLKMF 
      ALIIYKKEL IDLFTKGNH FWKLDKFGD CHKQKLTKL 
      HKYVDLFFY VYAVIITGA FLQLALLIL IFEPGKPIF 
      LCYGGLYGL ESPQFEFYA VLDFLAIGV IAISVTAYD 
      SIFFYFALY IYTEFKMIK IAFKRENCA QFIEAVKHH 
      DFLLQYISK VNEVFSVIF LTQFFSGLL GICFNLFMI 
      STQGTRDMK SFSTYFVGL VGYTAQSFT FCLIGELIS 
      ELSEDISNE IFYTDWLDD EVYRNTTAR LIVMNRAQE 
      SPKLTIGKF ADMNLRTFI IILRNAYSF LAFINEVLD
    CDS sequence
    • >TC030327 RA
      atggaaaac cctttaaaa ttgctacac atcataggc 
      ttagatcct cgccaaagt gataaatat tcaacaatt 
      aaaaaagtt atttctttt ctcatcgta ctggcagtg 
      ttgctgtca gccctaata gagtttttt ttgcatcat 
      aatgagtct caagtgtat gacactgca ccccaatcc 
      actgttccg aaccttcaa gcattgttg aagatgttt 
      gcattgata atttacaaa aaagaatta attgacttg 
      tttacaaaa ggcaatcat ttttggaaa ttggataaa 
      tttggtgat tgccacaag cagaaatta accaagtta 
      cacaaatac gtcgattta tttttttac gtttatgcg 
      gttattata actggggct tttctacaa cttgcactc 
      ctcatctta attttcgaa cccggaaaa cctattttt 
      ttgtgttat ggagggttg tatggactt gaatcaccc 
      cagtttgag ttttatgca gtgctggat ttcctggca 
      attggtgtg attgcaata tcagtaaca gcatacgac 
      agcatattc ttttacttc gctctgtac atttataca 
      gagtttaaa atgatcaaa attgctttt aagagagaa 
      aattgtgcc cagtttatc gaagctgtg aaacatcac 
      gatttttta ctacaatat attagtaaa gtgaacgag 
      gtgttttca gtgatattt ctaacacaa ttcttttcg 
      ggccttctt ggcatctgc ttcaactta ttcatgatt 
      tcaacacaa gggactcga gacatgaaa tcgttttcg 
      acttatttt gtcggtctt gtgggatac actgcccaa 
      tcattcacg ttctgtttg atcggagaa ttaatctca 
      gaattgtca gaagatatt tcaaacgaa attttttac 
      accgactgg cttgatgat gaggtttac agaaataca 
      actgcaaga cttattgta atgaacagg gcacaagag 
      tcaccaaaa ttgaccatt ggaaagttt gccgacatg 
      aatttgcga acttttata ataattcta cggaatgct 
      tattcgttt ttagcgttt attaacgag gtattagac 
      taa
    mRNA sequence
    • >TC030327 RA
      ttagtggcc atttttata aggtctagt agttaaaaa 
      cttaaaaat tcttcaaac agttcagtg tgaagcttt 
      ttctcgtat catttatgt attagaaca atttatttc 
      taaactacg attatagtt agaaacttt cttttcttc 
      aaaagcttc taaccaatt gtaaaataa gttggtgcc 
      agaatggaa aacccttta aaattgcta cacatcata 
      ggcttagat cctcgccaa agtgataaa tattcaaca 
      attaaaaaa gttatttct tttctcatc gtactggca 
      gtgttgctg tcagcccta atagagttt tttttgcat 
      cataatgag tctcaagtg tatgacact gcaccccaa 
      tccactgtt ccgaacctt caagcattg ttgaagatg 
      tttgcattg ataatttac aaaaaagaa ttaattgac 
      ttgtttaca aaaggcaat catttttgg aaattggat 
      aaatttggt gattgccac aagcagaaa ttaaccaag 
      ttacacaaa tacgtcgat ttatttttt tacgtttat 
      gcggttatt ataactggg gcttttcta caacttgca 
      ctcctcatc ttaattttc gaacccgga aaacctatt 
      tttttgtgt tatggaggg ttgtatgga cttgaatca 
      ccccagttt gagttttat gcagtgctg gatttcctg 
      gcaattggt gtgattgca atatcagta acagcatac 
      gacagcata ttcttttac ttcgctctg tacatttat 
      acagagttt aaaatgatc aaaattgct tttaagaga 
      gaaaattgt gcccagttt atcgaagct gtgaaacat 
      cacgatttt ttactacaa tatattagt aaagtgaac 
      gaggtgttt tcagtgata tttctaaca caattcttt 
      tcgggcctt cttggcatc tgcttcaac ttattcatg 
      atttcaaca caagggact cgagacatg aaatcgttt 
      tcgacttat tttgtcggt cttgtggga tacactgcc 
      caatcattc acgttctgt ttgatcgga gaattaatc 
      tcagaattg tcagaagat atttcaaac gaaattttt 
      tacaccgac tggcttgat gatgaggtt tacagaaat 
      acaactgca agacttatt gtaatgaac agggcacaa 
      gagtcacca aaattgacc attggaaag tttgccgac 
      atgaatttg cgaactttt ataataatt ctacggaat 
      gcttattcg tttttagcg tttattaac gaggtatta 
      gactaaatt acaaattga gattaaact gagtaaaaa 
      tac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY