Status
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Gene information
General information
TC032272
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030316,TC000166 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 15163233..15168660
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC032272
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09340

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Adult morphology
    abdominal odoriferous glands content darker - Number of animals showing the phenotype: 1 (13 dpi)
    Days post injection


    abdominal odoriferous glands content not present - Number of animals showing the phenotype: 3 (13 dpi)
    Days post injection


    abdominal odoriferous glands partially content not present - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032272 RA
      MDEEFLIGT FETEKKFLR YGSFYPCGK RIKFIFLGL 
FMFVYSWTE FLSMITVLF VERDNLTKL SETLLFCMT 
QAAFLFKLV NFLYHNKTM LRIESILKN PILNCLDQF 
EKNIIEKYM IRVKYLARL FRILCILTV SFYGLFPFI 
DEDPDHMLP LPGWFPFDV KTHQIELVI AQTCGIAIG 
AFLNSTLDI LPTILITLG SAQFDILKI RLENITSVD 
TSKSWLVKK AIKKCVIYH TILLNYITQ IEILFHKGI 
FVQFTASVV VICLTGFQM LVISVRSIQ FILLMIYFS 
TMTCQIALY CWYGNELMY RSMGLSDAC YMSEWNKCD 
TSVCKSLAI IMERGKRPV VLKAGNIFS LKLTTLMTV 
LNLGSSKMP FTIKDYDLR NAFETERTL LTLSGFYPR 
RTKKYNFFY NTSALINLF IAYGQLFSM VVQMVIDRN 
ELSKLSETL LFFMTHFTF LCKLTNFVY YKKKMFEIE 
DNLSRKIFY GFELWQIKP KIDSCKFIA KIFRILCIL 
VVLFYTLVP YLDDKEDLS LPLPGWLPY NTKKYYYPT 
VIFQVMSVS VSAYNNSSI DVLTCMLIT VASAEFNLL 
KGALKTIDF HPKGHNTKQ LIEAKFENC VNHHKEIVK 
FAYQIETIF SKGIFLQFF ASIIVICFT GFQMIVVPI 
PSMQFIFLI IYFSCMMCQ VAMYCWYGH DIITTSDSI 
GQAFYMSNW YESDVKIRK NICIFLERT KKPVILTAG 
KFVTLSLTT FTTILRSSY SYFAVLQHL YKEDS
    CDS sequence
    • >TC032272 RA
      atggacgaa gagttttta atcgggact tttgaaacg 
gaaaaaaaa ttccttcgt tatggaagt ttctatcct 
tgtggcaaa cgtatcaag tttattttt ttgggattg 
ttcatgttt gtctattcc tggactgaa ttcctctca 
atgatcaca gtattgttc gtcgaacgt gacaattta 
accaaatta tccgaaact ttgctcttt tgcatgact 
caagccgct tttctcttc aaacttgtc aattttctc 
taccacaac aagaccatg ctgcgaatt gaaagcatt 
cttaaaaac ccaattttg aattgtttg gaccaattt 
gaaaaaaac attattgag aagtacatg attcgagtg 
aaatatctg gcaaggttg ttccgaatt ttgtgcatt 
ttaactgtg tctttttat gggttgttt ccgtttatt 
gacgaggac ccagaccat atgttacca ctaccagga 
tggttccct ttcgatgtg aaaacccat caaattgaa 
ctggtcatt gcccagact tgtgggata gcaattggg 
gcttttctc aactcaacg ttggatatt ttacctacg 
attttaata actctaggt tctgcacaa ttcgatata 
ttgaaaata agactcgaa aatatcaca agtgtggat 
acttcgaag tcttggttg gtcaaaaaa gcaatcaaa 
aaatgcgtt atctatcac accatcctt ttaaactac 
attactcaa attgaaatt ttgttccac aaaggcatt 
tttgtgcaa tttacggca agtgttgta gttatttgt 
ctcacaggt ttccagatg cttgttatt tcggtcaga 
agtatccaa tttattctc ttgatgatt tatttctcg 
acaatgacg tgtcaaatt gcgctgtat tgttggtac 
ggcaatgaa ttaatgtac aggagtatg ggcttatct 
gatgcttgt tacatgtcc gagtggaac aaatgtgac 
acttcggtg tgtaaaagt ttggcaatt attatggaa 
agagggaaa agaccggtt gttttaaag gccgggaat 
attttttcg ctgaaactt acaacactg atgaccgta 
cttaacctt gggtcaagc aaaatgcct ttcaccatc 
aaagactac gatttgcga aatgccttt gaaactgaa 
cgcaccctt ttaaccctt tcagggttt taccctcgg 
cgtacgaaa aaatacaat tttttctac aacacaagt 
gccctcata aatttattc atcgcatat ggacagcta 
ttttcaatg gttgtccaa atggtaatc gaccgaaac 
gagctttcg aaattgagt gaaacgttg ctgtttttc 
atgacgcat tttacattt ttatgcaaa ctcacaaat 
ttcgtctac tacaagaaa aaaatgttt gaaattgaa 
gacaatctt tcaaggaag atattctac gggtttgaa 
ctgtggcag atcaaacct aaaattgac tcgtgcaag 
ttcatcgct aaaattttc cgaattttg tgcattttg 
gtggttttg ttttacaca cttgtgccc tatttggac 
gataaggag gatttgagt ttgcctttg ccgggctgg 
ttgccctac aacacgaaa aaatattac tacccgact 
gtaattttt caagtaatg agtgtgtcg gttagtgct 
tataataat tcaagcatt gatgttttg acttgtatg 
ttgatcacg gtcgcttcg gcggagttt aatttattg 
aaaggggcc ttaaagacg attgatttt cacccaaaa 
gggcacaat accaagcaa ctgattgaa gcaaaattt 
gaaaattgt gtcaatcat cacaaagaa attgtcaag 
tttgcttac caaatcgag accattttt tctaaaggg 
atttttttg caatttttt gcgagcatt attgtgatt 
tgtttcacc ggttttcag atgattgtt gtgcctatc 
ccaagtatg caatttatt tttttaatc atttatttc 
tcctgtatg atgtgtcag gtggcaatg tattgctgg 
tacggacac gatattata actacgagt gattcaatt 
gggcaagct ttttatatg tccaattgg tacgaatct 
gacgtcaaa attcgcaaa aatatttgc atttttttg 
gaaaggacg aaaaaaccg gttatttta acagcggga 
aaatttgtt accttgtca ctgaccacg ttcacaacc 
atcctacgg tcctcgtat tcgtatttt gcagtgctc 
caacattta tacaaagaa gattcttaa
    mRNA sequence
    • >TC032272 RA
      tattatgga aatatttca tggacgaag agtttttaa 
tcgggactt ttgaaacgg aaaaaaaat tccttcgtt 
atggaagtt tctatcctt gtggcaaac gtatcaagt 
ttatttttt tgggattgt tcatgtttg tctattcct 
ggactgaat tcctctcaa tgatcacag tattgttcg 
tcgaacgtg acaatttaa ccaaattat ccgaaactt 
tgctctttt gcatgactc aagccgctt ttctcttca 
aacttgtca attttctct accacaaca agaccatgc 
tgcgaattg aaagcattc ttaaaaacc caattttga 
attgtttgg accaatttg aaaaaaaca ttattgaga 
agtacatga ttcgagtga aatatctgg caaggttgt 
tccgaattt tgtgcattt taactgtgt ctttttatg 
ggttgtttc cgtttattg acgaggacc cagaccata 
tgttaccac taccaggat ggttccctt tcgatgtga 
aaacccatc aaattgaac tggtcattg cccagactt 
gtgggatag caattgggg cttttctca actcaacgt 
tggatattt tacctacga ttttaataa ctctaggtt 
ctgcacaat tcgatatat tgaaaataa gactcgaaa 
atatcacaa gtgtggata cttcgaagt cttggttgg 
tcaaaaaag caatcaaaa aatgcgtta tctatcaca 
ccatccttt taaactaca ttactcaaa ttgaaattt 
tgttccaca aaggcattt ttgtgcaat ttacggcaa 
gtgttgtag ttatttgtc tcacaggtt tccagatgc 
ttgttattt cggtcagaa gtatccaat ttattctct 
tgatgattt atttctcga caatgacgt gtcaaattg 
cgctgtatt gttggtacg gcaatgaat taatgtaca 
ggagtatgg gcttatctg atgcttgtt acatgtccg 
agtggaaca aatgtgaca cttcggtgt gtaaaagtt 
tggcaatta ttatggaaa gagggaaaa gaccggttg 
ttttaaagg ccgggaata ttttttcgc tgaaactta 
caacactga tgaccgtac ttaaccttg ggtcaagca 
aaatgcctt tcaccatca aagactacg atttgcgaa 
atgcctttg aaactgaac gcacccttt taacccttt 
cagggtttt accctcggc gtacgaaaa aatacaatt 
ttttctaca acacaagtg ccctcataa atttattca 
tcgcatatg gacagctat tttcaatgg ttgtccaaa 
tggtaatcg accgaaacg agctttcga aattgagtg 
aaacgttgc tgtttttca tgacgcatt ttacatttt 
tatgcaaac tcacaaatt tcgtctact acaagaaaa 
aaatgtttg aaattgaag acaatcttt caaggaaga 
tattctacg ggtttgaac tgtggcaga tcaaaccta 
aaattgact cgtgcaagt tcatcgcta aaattttcc 
gaattttgt gcattttgg tggttttgt tttacacac 
ttgtgccct atttggacg ataaggagg atttgagtt 
tgcctttgc cgggctggt tgccctaca acacgaaaa 
aatattact acccgactg taatttttc aagtaatga 
gtgtgtcgg ttagtgctt ataataatt caagcattg 
atgttttga cttgtatgt tgatcacgg tcgcttcgg 
cggagttta atttattga aaggggcct taaagacga 
ttgattttc acccaaaag ggcacaata ccaagcaac 
tgattgaag caaaatttg aaaattgtg tcaatcatc 
acaaagaaa ttgtcaagt ttgcttacc aaatcgaga 
ccatttttt ctaaaggga tttttttgc aattttttg 
cgagcatta ttgtgattt gtttcaccg gttttcaga 
tgattgttg tgcctatcc caagtatgc aatttattt 
ttttaatca tttatttct cctgtatga tgtgtcagg 
tggcaatgt attgctggt acggacacg atattataa 
ctacgagtg attcaattg ggcaagctt tttatatgt 
ccaattggt acgaatctg acgtcaaaa ttcgcaaaa 
atatttgca tttttttgg aaaggacga aaaaaccgg 
ttattttaa cagcgggaa aatttgtta ccttgtcac 
tgaccacgt tcacaacca tcctacggt cctcgtatt 
cgtattttg cagtgctcc aacatttat acaaagaag 
attcttaat tgtctgatt taattatga acatttttc 
acgtgcact tcaactagc ac

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY