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Gene information
General information
TC034229
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030267
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 4603419..4611866
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034229
    iBeetle screen iB_09339

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034229 RA
      MVYSWDSVA KEANKTGLL VHKYWNEFH 
      ENTTITSEN DDNFGTISL QLLQSKLKF 
      TVCGFVDLD WSLIHLIIQ TTAMYLVWI 
      IQLKS
    CDS sequence
    • >TC034229 RA
      atggtttat tcctgggat tcggtggcc aaagaggcc 
      aataaaacc ggattactt gtacacaaa tactggaac 
      gaatttcac gaaaatacc acaataacg agcgaaaac 
      gacgacaac tttggcaca atttccctg cagttgtta 
      caaagcaaa ctgaaattt acagtgtgt gggtttgtg 
      gacttggat tggtccttg atacacttg attattcaa 
      acaacagca atgtatctg gtctggatt atacaactc 
      aaatcataa
    mRNA sequence
    • >TC034229 RA
      ttctgatgt cctcgcaaa acagtcctc acctcatct 
      aattgattg cagcgcgtt tgatgtgtt gggacgctt 
      caataaaac cagccggaa ttaatttaa atcctttat 
      tatttggaa cgaaatcac ttacaacta gacactaca 
      cgaaatcca aactcaaaa ctcctgtgt aaaaataat 
      tatgaactt aatatctgc cgctgcgcc ccgagcgat 
      ggcgtgggg agcgagacc ttctttccc tcgaccgag 
      aacgtcttc ttggtgatt tagacctag aacgcctgc 
      ggccagatt ttcgccgag aatacaagt atctgaaac 
      aatatttcg aaatcgtat cgaacttcc ttcttccag 
      caactcaac ccatcacaa actcttaat ttaattgca 
      ttacataat caattacac ggtcaaaca tgctcacgt 
      cgaagccgg ttcattata ttaagcatg aaccgtaat 
      accttaatg tatccatta ataaataag cagtcggat 
      attattgat atgttgatg tgtcgcagg agcaaaacc 
      attatcgac agttttaat aatgacgcc aacattcac 
      gatccattt aaagccgct gtattattt agcgagatt 
      tttcggcat cttccattt ccagttgta cgaccaaaa 
      caaagcgaa aatttccac acgagtgct gaatcattt 
      ctggagcgt accgtacat tacatatca cgtaacact 
      cattttctt acaattctt cttttttca acacaaaaa 
      tggtccgag atacaaaac ggttagaaa aatgctctt 
      cgcaaccgg gtgttttct taaattaac cgccgacta 
      tgtgtggtt tgtcacaaa gcgtcacca actcaaaaa 
      aatgcttca cgatattaa tttagtttg ccaaagaaa 
      caccgtcac attcaataa cacaaaact aaaaattca 
      aatcagttt aattatcgc gctaatagt ggttttagg 
      gaccggtat cgcaatcac aagttgctt acacggttt 
      caacctttg gctaatttg gatgaaagt aagtttcgt 
      atcactggg cctaacaaa ttttgttat gcacatcat 
      aaacaaagc catcaaaga cttattttc aacaatcaa 
      caacaacct cgataactt aaaacacac gacaccgaa 
      cgagaccgt aacaatttt taaaacaaa tacggaaat 
      tcattccca aataacgca acttgcgag acacacgaa 
      ttcaatgtt tgcggttcc tttactggg aaactcact 
      tctttcact cattctgac ggtattttt cacatacca 
      atttattgt tcgtgttaa cgaaaaagt tccacgtgt 
      ttattttct tgtacaaga cactgtaaa cctaactct 
      gcttgcatt tgaaaccct gggtatggt ttattcctg 
      ggattcggt ggccaaaga ggccaataa aaccggatt 
      acttgtaca caaatactg gaacgaatt tcacgaaaa 
      taccacaat aacgagcga aaacgacga caactttgg 
      cacaatttc cctgcagtt gttacaaag caaactgaa 
      atttacagt gtgtgggtt tgtggactt ggattggtc 
      cttgataca cttgattat tcaaacaac agcaatgta 
      tctggtctg gattataca actcaaatc ataaacaat 
      taaataata aatttattt ttcaatagt ttttaactt 
      accttcgca actcgcgag aaatcggct tcaggtgca 
      tgaaattgc aaaaacctg agcgcgtac actccccca 
      tttcgtact ggcgacagc aagcttccc taaagtcag 
      tcacggggc tcagctcgg cataaacag gtcgccccc 
      cgaaccaac gattattca aatcgttaa cagccctct 
      cagcgtctt cctcacgtc gaaactacc aaactatgt 
      attgacgag acacaagtt tactgaaac ttactttaa 
      tgtagacat ttccgacaa ggtggtccc ccaaattat 
      cgcacacgt tcatttcct caatttccc cgtacttat 
      cttcacatt ctacgaaaa cgtcctcga aaaagttat 
      cgtaatgct cttgcatct cttcgtcag agacgtttg 
      caaccactg aaatttgga tcgtgaaaa gtggacaca 
      aacctgagg ggaacttac agtgactcc catcggcag 
      atttcgcgg agttttggg gattcacgt acaaattct 
      gcaaaagac acgtttggg aaaacgttg gtttgttgt 
      gtattctgg aacaacgat cgccatgcc ggcaagccc 
      cgattttga agtagaaag agcagttga ctcta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY