Status
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Gene information
General information
TC030243 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10736497..10738381
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030243
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09337

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Adult morphology
    abdominal odoriferous glands colour darker - Number of animals showing the phenotype: 6 (13 dpi)
    Days post injection



    prothoracic odoriferous glands colour darker - Number of animals showing the phenotype: 5 (13 dpi)
    Days post injection


    abdominal odoriferous glands partially content darker - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    abdominal odoriferous glands content not present - Number of animals showing the phenotype: 1 (13 dpi)
    Days post injection


    abdominal odoriferous glands structure irregular - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030243 RA
      MSTQDIYDA VYPLLLTTA SFGLSAIFV ETKNNTRKL 
      AVFNLLKIL NIIYLSLFS ALLYVAFTS LKNTHLHVN 
      YNSGVTKIG IIFQILANI MATYVIYFI NISKSHNIL 
      TCIENIKKA DLMFRDLGE KIIYRKHFL YEVCMILFG 
      VLAILGRSI VTHIYMGRN IFLTENETH FALFFPLFV 
      SYLVQINFV LLITLVHER FALINRLLD NFNEEKVKH 
      NYLSLKPPY EYQTKYNQA KIELLMELH DFLTDVGNK 
      LNDSFSIQI LSCLTSQFL TEVFTIFYL YYESLILKN 
      KIAALVWLM WSIWTTLEI FYVTVNCHL TTKEAKNTG 
      IAIHKVLMN ESDPDAKRK LMVFSQQVN HRSLQFTAC 
      GLFYIDATL IFTIVGAAA TYLLIMLQF QEGIEAQCS 
      NNTLLN
    CDS sequence
    • >TC030243 RA
      atgtccaca caagatatt tacgatgct gtctaccct 
      cttttactc acaactgca tcatttggt ctgtctgca 
      attttcgtg gaaacgaaa aataacact cggaaactg 
      gcagttttc aacttactc aaaatcctg aacataatt 
      tacctttcg ctattttca gcactgttg tatgttgct 
      tttaccagt ttaaaaaac acacacctt cacgtaaat 
      tacaactct ggtgtcact aaaatcggg ataattttc 
      caaattctt gcaaatatt atggctact tatgtgatc 
      tactttatt aacatatca aagagccac aacatatta 
      acctgtatc gagaacatc aaaaaagct gatttgatg 
      ttcagggac ttgggagag aaaattatt tatcgcaaa 
      cactttttg tacgaagtt tgcatgatt ttgtttgga 
      gttctggcg attttggga cgaagcatt gttacgcac 
      atctacatg gggagaaac atttttctg acagagaac 
      gaaactcat tttgcactc tttttccct ctattcgtg 
      tcttatttg gtgcaaatt aactttgtg ctattaatc 
      acgctggta cacgaacgt ttcgcgttg attaataga 
      ctactggat aattttaat gaagaaaaa gtgaaacat 
      aattacctc agtttgaaa ccaccgtat gaatatcaa 
      acgaaatat aatcaagca aaaattgaa ttgttaatg 
      gaacttcac gatttctta accgatgtg ggcaataaa 
      ttaaacgac agtttttca atccaaatt ctgagctgc 
      ctgacctca caatttcta actgaagtt tttacgatt 
      ttctacttg tattatgaa agtttgatt ttgaaaaac 
      aaaattgca gctcttgtc tggttaatg tggtcaata 
      tggacaact cttgagata ttttacgta acagtaaac 
      tgtcattta actaccaaa gaagcaaaa aatacagga 
      atagccatt cacaaagtt cttatgaat gagtctgat 
      cctgacgca aaaagaaaa ttaatggta ttttcgcaa 
      caagtgaat cacagatcc ttacagttt acagcctgt 
      ggtttattt tacatcgac gcaactcta atttttaca 
      atagttggg gctgcagcc acttacctg ctgataatg 
      ctacagttt caggaagga attgaagca caatgttca 
      aataatact ctcctaaat taa
    mRNA sequence
    • >TC030243 RA
      tttccgtag ttttaaaat gtccacaca agatattta 
      cgatgctgt ctaccctct tttactcac aactgcatc 
      atttggtct gtctgcaat tttcgtgga aacgaaaaa 
      taacactcg gaaactggc agttttcaa cttactcaa 
      aatcctgaa cataattta cctttcgct attttcagc 
      actgttgta tgttgcttt taccagttt aaaaaacac 
      acaccttca cgtaaatta caactctgg tgtcactaa 
      aatcgggat aattttcca aattcttgc aaatattat 
      ggctactta tgtgatcta ctttattaa catatcaaa 
      gagccacaa catattaac ctgtatcga gaacatcaa 
      aaaagctga tttgatgtt cagggactt gggagagaa 
      aattattta tcgcaaaca ctttttgta cgaagtttg 
      catgatttt gtttggagt tctggcgat tttgggacg 
      aagcattgt tacgcacat ctacatggg gagaaacat 
      ttttctgac agagaacga aactcattt tgcactctt 
      tttccctct attcgtgtc ttatttggt gcaaattaa 
      ctttgtgct attaatcac gctggtaca cgaacgttt 
      cgcgttgat taatagact actggataa ttttaatga 
      agaaaaagt gaaacataa ttacctcag tttgaaacc 
      accgtatga atatcaaac gaaatataa tcaagcaaa 
      aattgaatt gttaatgga acttcacga tttcttaac 
      cgatgtggg caataaatt aaacgacag tttttcaat 
      ccaaattct gagctgcct gacctcaca atttctaac 
      tgaagtttt tacgatttt ctacttgta ttatgaaag 
      tttgatttt gaaaaacaa aattgcagc tcttgtctg 
      gttaatgtg gtcaatatg gacaactct tgagatatt 
      ttacgtaac agtaaactg tcatttaac taccaaaga 
      agcaaaaaa tacaggaat agccattca caaagttct 
      tatgaatga gtctgatcc tgacgcaaa aagaaaatt 
      aatggtatt ttcgcaaca agtgaatca cagatcctt 
      acagtttac agcctgtgg tttatttta catcgacgc 
      aactctaat ttttacaat agttggggc tgcagccac 
      ttacctgct gataatgct acagtttca ggaaggaat 
      tgaagcaca atgttcaaa taatactct cctaaatta 
      actagttac cttcagttt aggcacagc cccctggcg 
      tttctcatc atttttaaa attcaagag acatttttt 
      tacttcttg taatttttc cattgaagt tgttgaagg 
      tagtgtgaa actagactc attctaggg tgattttga 
      acgtaataa ataaatata aaacaatat gtgtcgtac 
      ctcttgaaa gcccattaa tttcaaaca ttccgaacc 
      tcccttcac ggacaaaat tttattttt tattgtgga 
      attgctttt ttgtagaga tcgtacttt taggttagc 
      tggatcttt aacaatatt ttcggctat tttaactag 
      gagacgtac agcctcatc acagttttg ttccgagtt 
      ggatcttag cttcgaaaa cacttcttc tgcactcat 
      tttaacaca ttaattagt taaaccgct gattaattc 
      ttttttttg gatctgtca acgttgacg ttctcatga 
      tc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY