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Gene information
General information
TC030194 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4059326..4060956
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030194
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09334

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030194 RA
      MACRDLPLQ TIYKISENL CMFPAYNFA KSQIEKARF 
      RKIQGCLII FIIILAIIV SLYMRHYHF KGLFTPTHL 
      TLDNLNELF LTGVALGLV IRTCFGKTD HWFQLQYNL 
      QTLDKILGN CSEDGNFWK NIYVQLILY GLIFVYGIY 
      YMVLMMVVE KVGSWMDFV YVVHEFCYL YDILVHFLF 
      INIASNLHT KYKSMNRLF NFAKTTDSF RKLGTISRV 
      LAETVHAFN TTFGWVFLL IIAKNVNQI LRCLNYILN 
      NTFNLSISN TILGIYISL TLASIMLSC DFVVTESRK 
      SVFLCFKLQ EQFESLVDR NELFNLGKQ LEANTVTFT 
      AAGFFEINR SALFAIFGT VATYSLVLV QFNGDFANQ 
      QNCTCLQ
    CDS sequence
    • >TC030194 RA
      atggcttgt cgagacctg cctttacaa acaatttac 
      aaaatttcc gaaaacttg tgcatgttt ccagcgtac 
      aattttgca aaatcccag atcgaaaaa gccagattt 
      cgtaaaatc caaggctgt ttgataata tttattatc 
      atacttgca ataatagtt tcgctctac atgagacat 
      tatcacttt aaaggcctg ttcacaccc actcacctc 
      actttagat aacttgaac gaattgttt ttgacgggt 
      gtggccttg ggtctcgtc atccggact tgtttcgga 
      aaaacggac cactggttc caattacag tataatttg 
      caaacctta gataaaatt ttgggtaat tgctccgaa 
      gatggaaat ttttggaaa aatatttac gtacaattg 
      attttgtat ggtttgatt ttcgtttac gggatttat 
      tacatggta cttatgatg gtggtggaa aaagtgggc 
      tcttggatg gacttcgta tacgtggtc cacgaattt 
      tgttattta tacgacatc ctggtccat tttttgttc 
      ataaatatt gcgtcaaac ttgcacact aaatacaaa 
      tctatgaac cggcttttc aattttgca aaaacaact 
      gattctttc cgaaaactt ggaacaata tcgcgagtt 
      ttggccgaa actgtgcat gcttttaat acgactttt 
      ggatgggtt ttccttcta ataattgcg aaaaacgta 
      aatcaaatc ctccgttgt ttaaactac attttgaac 
      aacaccttc aacttatcc atttctaac acaatctta 
      ggaatctat atttcgtta actctcgct tctattatg 
      ttatcctgc gatttcgtg gtcacagaa agtcgaaaa 
      tcggttttt ctatgtttt aaattgcaa gaacaattc 
      gagagtctt gtcgaccga aacgaattg tttaatttg 
      gggaaacaa ctggaggcg aatacggtt actttcaca 
      gcggctggt tttttcgag attaatcga agtgccctt 
      tttgctatt tttgggacc gtggctacg tattctctc 
      gtcctggtc cagtttaat ggcgatttc gcgaatcaa 
      caaaactgt acctgcttg caataa
    mRNA sequence
    • >TC030194 RA
      taaattttc gacaagacg gtaattaaa attgtttca 
      gggaagaat ccataattt tttttcaaa attttcaat 
      cgtttcttg aaaaattat tccaacaat ggcttgtcg 
      agacctgcc tttacaaac aatttacaa aatttccga 
      aaacttgtg catgtttcc agcgtacaa ttttgcaaa 
      atcccagat cgaaaaagc cagatttcg taaaatcca 
      aggctgttt gataatatt tattatcat acttgcaat 
      aatagtttc gctctacat gagacatta tcactttaa 
      aggcctgtt cacacccac tcacctcac tttagataa 
      cttgaacga attgttttt gacgggtgt ggccttggg 
      tctcgtcat ccggacttg tttcggaaa aacggacca 
      ctggttcca attacagta taatttgca aaccttaga 
      taaaatttt gggtaattg ctccgaaga tggaaattt 
      ttggaaaaa tatttacgt acaattgat tttgtatgg 
      tttgatttt cgtttacgg gatttatta catggtact 
      tatgatggt ggtggaaaa agtgggctc ttggatgga 
      cttcgtata cgtggtcca cgaattttg ttatttata 
      cgacatcct ggtccattt tttgttcat aaatattgc 
      gtcaaactt gcacactaa atacaaatc tatgaaccg 
      gcttttcaa ttttgcaaa aacaactga ttctttccg 
      aaaacttgg aacaatatc gcgagtttt ggccgaaac 
      tgtgcatgc ttttaatac gacttttgg atgggtttt 
      ccttctaat aattgcgaa aaacgtaaa tcaaatcct 
      ccgttgttt aaactacat tttgaacaa caccttcaa 
      cttatccat ttctaacac aatcttagg aatctatat 
      ttcgttaac tctcgcttc tattatgtt atcctgcga 
      tttcgtggt cacagaaag tcgaaaatc ggtttttct 
      atgttttaa attgcaaga acaattcga gagtcttgt 
      cgaccgaaa cgaattgtt taatttggg gaaacaact 
      ggaggcgaa tacggttac tttcacagc ggctggttt 
      tttcgagat taatcgaag tgccctttt tgctatttt 
      tgggaccgt ggctacgta ttctctcgt cctggtcca 
      gtttaatgg cgatttcgc gaatcaaca aaactgtac 
      ctgcttgca ataaaattg ccacaaatt gattgtttt 
      ttatttacc caaaatgac aggtaatgg caattaact 
      gatagaaga gaaaaattt cacttataa ataggggag 
      agaaactaa tggattagt gtgtttgcc gtgttagtg 
      caatgagtt cgaagaaag atatccgtt ttatgaaaa 
      ttccctaca ctattggca gctatctgg ctgtgaccc 
      cggactaca attttgaaa aaaaagtca taaagcggc 
      ccagagttc accaaattt acgcatttt tgctcgcgt 
      gtgctatat ctgtattaa gtgggataa cagttgtgg 
      ataatgcta cactcagta tgaaacaac aagtaccga 
      ctgttttca tcctcgatt taataaatt tcttttttc 
      tat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY