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Gene information
General information
TC030191 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 4071553..4072791
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030191
    iBeetle screen iB_09333

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030191 RA
      MSYRLSRND IRFLKVMYK LSHFLSITP NYDFENFVI 
      ISPRCDKIS AICFLLSTI LGTCWIIYV RIYCKEIRF 
      FEISFEILG SLDSLILLM VLVSIILGS LKTKEWAKL 
      NNKFQYIDE KLKTRDQKE RNLFKNAYF QLVLSISVY 
      SSSVGYTQY VRIAAKGLP TLKLFALHE FYGFCYLWV 
      LILICNIAL AFKQRYQLI NEQMAVRIN PKNCASFVI 
      EVRKLSRLL GEMVGLFND IFGWPLVFI TGRFVIKIL 
      VALNFFTST LEIDNLYLK HKLEISSLV QTAITLVAM 
      CGLVLICDS AKSESQQTV FLCYKLMEK FPERSHEQQ 
      ELYMAAQVI EKSVANFTA AGFFDVKRS TLFGILATT 
      TTYLIVTIQ FNQGLNK
    CDS sequence
    • >TC030191 RA
      atgtcatac agactgtct cgcaatgat attcgcttc 
      ttgaaagta atgtacaaa cttagccat tttttgtca 
      atcacacca aactacgat ttcgaaaat ttcgtgatc 
      atttcgcct cgatgtgac aaaatttct gcaatttgt 
      ttcctactt tcgacaatt ttaggcact tgttggata 
      atctacgtg cgcatttac tgcaaagaa atacgtttt 
      ttcgaaata tcgttcgaa atattgggc tctttggac 
      agtttaatt ctcttgatg gtgctcgtg tcgattatt 
      ttaggttcg cttaaaaca aaggaatgg gcgaaattg 
      aacaacaag ttccaatat atcgacgaa aaactcaag 
      acgcgagac cagaaagaa cgaaatttg tttaaaaat 
      gcctatttc cagctcgtt ttgtccatt tctgtttac 
      agttcttca gttggctac actcaatat gtgcgtatc 
      gctgcgaag ggtttgcca actttgaaa ctgttcgct 
      ttacatgaa ttttacggt ttttgctat ttgtgggtc 
      ctgatttta atttgcaac atcgcactt gcgttcaag 
      cagaggtac cagctgata aatgaacag atggctgtt 
      cgaattaat ccgaaaaat tgtgcgagt tttgtgatc 
      gaagtccga aaattgtcg cgacttttg ggcgaaatg 
      gtcggtttg tttaatgac atttttggt tggccgctg 
      gtttttatc actgggcgg ttcgtgata aagatttta 
      gtcgcgtta aattttttt acaagcact ttggagatc 
      gataatttg tacttgaaa cacaagctt gaaatttcg 
      agtcttgtc cagacggca attacgctg gtcgctatg 
      tgtggtttg gttttaatt tgtgactcg gccaagtct 
      gagagtcaa caaactgtt ttcctgtgt tacaaatta 
      atggaaaaa ttcccggaa aggtcgcac gaacaacaa 
      gagttgtac atggcggca caggtgatt gagaaaagt 
      gttgccaac tttacggcc gctggtttt ttcgatgtt 
      aagcggagc acactgttt gggattttg gcaacgacg 
      accacttat ttgattgtc acgattcag ttcaatcaa 
      ggtttaaat aaataa
    mRNA sequence
    • >TC030191 RA
      aatgaattg tttcattat tataatatt aaactgtaa 
      aatgtcata cagactgtc tcgcaatga tattcgctt 
      cttgaaagt aatgtacaa acttagcca ttttttgtc 
      aatcacacc aaactacga tttcgaaaa tttcgtgat 
      catttcgcc tcgatgtga caaaatttc tgcaatttg 
      tttcctact ttcgacaat tttaggcac ttgttggat 
      aatctacgt gcgcattta ctgcaaaga aatacgttt 
      tttcgaaat atcgttcga aatattggg ctctttgga 
      cagtttaat tctcttgat ggtgctcgt gtcgattat 
      tttaggttc gcttaaaac aaaggaatg ggcgaaatt 
      gaacaacaa gttccaata tatcgacga aaaactcaa 
      gacgcgaga ccagaaaga acgaaattt gtttaaaaa 
      tgcctattt ccagctcgt tttgtccat ttctgttta 
      cagttcttc agttggcta cactcaata tgtgcgtat 
      cgctgcgaa gggtttgcc aactttgaa actgttcgc 
      tttacatga attttacgg tttttgcta tttgtgggt 
      cctgatttt aatttgcaa catcgcact tgcgttcaa 
      gcagaggta ccagctgat aaatgaaca gatggctgt 
      tcgaattaa tccgaaaaa ttgtgcgag ttttgtgat 
      cgaagtccg aaaattgtc gcgactttt gggcgaaat 
      ggtcggttt gtttaatga catttttgg ttggccgct 
      ggtttttat cactgggcg gttcgtgat aaagatttt 
      agtcgcgtt aaatttttt tacaagcac tttggagat 
      cgataattt gtacttgaa acacaagct tgaaatttc 
      gagtcttgt ccagacggc aattacgct ggtcgctat 
      gtgtggttt ggttttaat ttgtgactc ggccaagtc 
      tgagagtca acaaactgt tttcctgtg ttacaaatt 
      aatggaaaa attcccgga aaggtcgca cgaacaaca 
      agagttgta catggcggc acaggtgat tgagaaaag 
      tgttgccaa ctttacggc cgctggttt tttcgatgt 
      taagcggag cacactgtt tgggatttt ggcaacgac 
      gaccactta tttgattgt cacgattca gttcaatca 
      aggtttaaa taaataaaa gaaagtcgg tggaataat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY