Status
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Gene information
General information
TC030143 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007421.3: 55779..61104
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030143
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09332

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030143 RA
      MSHRLFELF FNIGKFLSL TPSSTNNQN PSCVQKLYE 
      ISVFLAYLG GRLFLLITS TDGIFTNLT SIQLVLAIM 
      SITNQFFFI LYIVIIVMR FKRQCWFKL IQNLATIHD 
      TMRLKWYYI TISMAFIIF VSLEIYHIY TVVEESGFE 
      NFVKTYLVS DFENYVTFF CSLFIVVIL KLILAKYRQ 
      QDVTLLQIT KAKNLLYSK QIVTILKKT KSNIFILKE 
      SVDTFNDIF GWIILCNIF EAAAKSLIY IDMIIKKNV 
      TQQNSDVFI FDNVWLFIL WVGILSTIF LCDTILKKV 
      DDILSQAYK LEASFDDLA TYETDEVQI FIDVVQHNR 
      PEFKAARFF SIDRSTLFS VLNSLTTFL LVMIQFKEN
    • >TC030143 RB
      MRLKWYYIT ISMAFIIFV SLEIYHIYT VVEESGFEN 
      FVKTYLVSD FENYVTFFC SLFIVVILK LILAKYRQQ 
      DVTLLQITK AKNLLYSKQ IVTILKKTK SNIFILKES 
      VDTFNDIFG WIILCNIFE AAAKSLIYI DMIIKKNVT 
      QQNSDVFIF DNVWLFILW VGILSTIFL CDTILKKVD 
      DILSQAYKL EASFDDLAT YETDEVQIF IDVVQHNRP 
      EFKAARFFS IDRSTLFSV LNSLTTFLL VMIQFKEN
    CDS sequence
    • >TC030143 RA
      atgagccac cgcttattc gaactgttt ttcaacatt 
      ggaaaattt ctatcacta actccatcc tcgaccaat 
      aaccaaaat ccatcatgt gtacaaaaa ttgtacgaa 
      atttcggtt tttctcgca tatttaggc ggacgtctg 
      tttctactt attacttca acggatgga attttcaca 
      aacttgacg tcaatacaa ctcgttttg gccataatg 
      agtatcaca aaccagttt ttctttatt ttgtatatt 
      gtgataatt gtgatgcga tttaaacga caatgttgg 
      ttcaaactt attcaaaat ttggctaca attcacgac 
      acaatgcgt ttgaaatgg tattacatc acaatatca 
      atggctttc ataattttc gtctcgttg gaaatttat 
      cacatttat accgtggtt gaagagtcg ggttttgaa 
      aactttgta aaaacgtat cttgtgtcc gattttgaa 
      aattacgtc acttttttc tgctctctc ttcattgtt 
      gtgatactt aaattgatt ttggccaaa tatcggcaa 
      caagatgtc acgctgctt cagatcaca aaagcgaaa 
      aatttacta tactcgaaa cagattgtc acaattttg 
      aaaaaaacc aaatcaaat attttcatc ttgaaggaa 
      agtgttgat acttttaat gatattttc ggctggatt 
      attttgtgt aatatattc gaagcggcc gcaaaaagc 
      cttatttac atcgacatg attatcaag aaaaatgtg 
      acgcaacaa aatagtgac gtgttcatt ttcgataat 
      gtgtggctt tttattctt tgggtgggc attttatca 
      acaattttt ctctgtgac acaatttta aaaaaagtg 
      gacgatatt ttgtcacaa gcgtacaaa ctcgaagcc 
      tcctttgat gaccttgca acgtacgaa acggacgaa 
      gtccaaatt tttatcgac gtagtccaa cataatcgt 
      cctgaattc aaagcggcg cgatttttc tcaatcgat 
      cgttcaaca cttttcagc gttttgaac tcactgaca 
      acatttctt ctcgtaatg atccaattt aaagaaaat 
      tga
    • >TC030143 RB
      atgcgtttg aaatggtat tacatcaca atatcaatg 
      gctttcata attttcgtc tcgttggaa atttatcac 
      atttatacc gtggttgaa gagtcgggt tttgaaaac 
      tttgtaaaa acgtatctt gtgtccgat tttgaaaat 
      tacgtcact tttttctgc tctctcttc attgttgtg 
      atacttaaa ttgattttg gccaaatat cggcaacaa 
      gatgtcacg ctgcttcag atcacaaaa gcgaaaaat 
      ttactatac tcgaaacag attgtcaca attttgaaa 
      aaaaccaaa tcaaatatt ttcatcttg aaggaaagt 
      gttgatact tttaatgat attttcggc tggattatt 
      ttgtgtaat atattcgaa gcggccgca aaaagcctt 
      atttacatc gacatgatt atcaagaaa aatgtgacg 
      caacaaaat agtgacgtg ttcattttc gataatgtg 
      tggcttttt attctttgg gtgggcatt ttatcaaca 
      atttttctc tgtgacaca attttaaaa aaagtggac 
      gatattttg tcacaagcg tacaaactc gaagcctcc 
      tttgatgac cttgcaacg tacgaaacg gacgaagtc 
      caaattttt atcgacgta gtccaacat aatcgtcct 
      gaattcaaa gcggcgcga tttttctca atcgatcgt 
      tcaacactt ttcagcgtt ttgaactca ctgacaaca 
      tttcttctc gtaatgatc caatttaaa gaaaattga
    mRNA sequence
    • >TC030143 RA
      gtaatccgc catgagcca ccgcttatt cgaactgtt 
      tttcaacat tggaaaatt tctatcact aactccatc 
      ctcgaccaa taaccaaaa tccatcatg tgtacaaaa 
      attgtacga aatttcggt ttttctcgc atatttagg 
      cggacgtct gtttctact tattacttc aacggatgg 
      aattttcac aaacttgac gtcaataca actcgtttt 
      ggccataat gagtatcac aaaccagtt tttctttat 
      tttgtatat tgtgataat tgtgatgcg atttaaacg 
      acaatgttg gttcaaact tattcaaaa tttggctac 
      aattcacga cacaatgcg tttgaaatg gtattacat 
      cacaatatc aatggcttt cataatttt cgtctcgtt 
      ggaaattta tcacattta taccgtggt tgaagagtc 
      gggttttga aaactttgt aaaaacgta tcttgtgtc 
      cgattttga aaattacgt cactttttt ctgctctct 
      cttcattgt tgtgatact taaattgat tttggccaa 
      atatcggca acaagatgt cacgctgct tcagatcac 
      aaaagcgaa aaatttact atactcgaa acagattgt 
      cacaatttt gaaaaaaac caaatcaaa tattttcat 
      cttgaagga aagtgttga tacttttaa tgatatttt 
      cggctggat tattttgtg taatatatt cgaagcggc 
      cgcaaaaag ccttattta catcgacat gattatcaa 
      gaaaaatgt gacgcaaca aaatagtga cgtgttcat 
      tttcgataa tgtgtggct ttttattct ttgggtggg 
      cattttatc aacaatttt tctctgtga cacaatttt 
      aaaaaaagt ggacgatat tttgtcaca agcgtacaa 
      actcgaagc ctcctttga tgaccttgc aacgtacga 
      aacggacga agtccaaat ttttatcga cgtagtcca 
      acataatcg tcctgaatt caaagcggc gcgattttt 
      ctcaatcga tcgttcaac acttttcag cgttttgaa 
      ctcactgac aacatttct tctcgtaat gatccaatt 
      taaagaaaa ttgacttgt aatttgttt atgccagtc 
      cgaaaaatg gacttgcaa gtggttgac ttgattttc 
      aaagttggc aaaatcttc acaataaca ccgtctagt 
      ttattacaa acgagtcca aataaaatt cacagttgt 
      tt
    • >TC030143 RB
      attttcact gatgtcact cgctacgtt cacaatctc 
      tacattttt ctttctgtg atgattttt aaacgcaag 
      ttctgaata ttttgacac aagttttct taaaatatt 
      caaatattg ttcaacttc gtgaatgtt gtattggaa 
      ctgtccgaa aaaatggac caagaccat cataggttc 
      ttgcacttt tacaatacc aagaatttc aataaaagt 
      atcaaaaat cgggtatgt tttaacaca tgacatgtc 
      taacaatac tttacaatt atctttaaa agttaaata 
      atggcttcc tccacagta tttatacat tcccacaag 
      ttttacttt tttatttgg gggcaaatt atcaaccag 
      tgttttgat ttcgcgaaa ataataaat attcaagct 
      tctttttac gcaaggctg taacgtcgt tattttact 
      ttgtagtgg tgcctccaa catttcgtc catttcttc 
      atccgtaat ttcctcttc catcttaat cctgtaaca 
      ataaaataa tgatcaatt ttcattcaa atttcggtg 
      tttaaacca aaacatgtt taccaaaac taatttcgt 
      gtattttcc actaaaagc cttctgttt gtccactca 
      acaacactt aatgtttat ctttgtaat gtgtataat 
      tttccatcg attagacgt tataaacaa attattttt 
      ccaactata gccttgtaa tccgccatg agccaccgc 
      ttattcgaa ctgtttttc aacattgga aaatttcta 
      tcactaact ccatcctcg accaataac caaaatcca 
      tcatgtgta caaaaattg tacgaaatt tcggttttt 
      ctcgcatat ttaggcgga cgtctgttt ctacttatt 
      acttcaacg gatggaatt ttcacaaac ttgacgtca 
      atacaactc gttttggcc ataatgagt atcacaaac 
      cagtttttc tttattttg tatattgtg ataattgtg 
      atgcgattt aaacgacaa tgttggttc aaacttatt 
      caaaatttg gctacaatt cacgacaca atgcgtttg 
      aaatggtat tacatcaca atatcaatg gctttcata 
      attttcgtc tcgttggaa atttatcac atttatacc 
      gtggttgaa gagtcgggt tttgaaaac tttgtaaaa 
      acgtatctt gtgtccgat tttgaaaat tacgtcact 
      tttttctgc tctctcttc attgttgtg atacttaaa 
      ttgattttg gccaaatat cggcaacaa gatgtcacg 
      ctgcttcag atcacaaaa gcgaaaaat ttactatac 
      tcgaaacag attgtcaca attttgaaa aaaaccaaa 
      tcaaatatt ttcatcttg aaggaaagt gttgatact 
      tttaatgat attttcggc tggattatt ttgtgtaat 
      atattcgaa gcggccgca aaaagcctt atttacatc 
      gacatgatt atcaagaaa aatgtgacg caacaaaat 
      agtgacgtg ttcattttc gataatgtg tggcttttt 
      attctttgg gtgggcatt ttatcaaca atttttctc 
      tgtgacaca attttaaaa aaagtggac gatattttg 
      tcacaagcg tacaaactc gaagcctcc tttgatgac 
      cttgcaacg tacgaaacg gacgaagtc caaattttt 
      atcgacgta gtccaacat aatcgtcct gaattcaaa 
      gcggcgcga tttttctca atcgatcgt tcaacactt 
      ttcagcgtt ttgaactca ctgacaaca tttcttctc 
      gtaatgatc caatttaaa gaaaattga cttgtaatt 
      tgtttatgc cagtccgaa aaatggact tgcaagtgg 
      ttgacttga ttttcaaag ttggcaaaa tcttcacaa 
      taacaccgt ctagtttat tacaaacga gtccaaata 
      aaattcaca gttgttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY