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Gene information
General information
TC032787
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC030134
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 3220778..3223515
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032787
    iBeetle screen iB_09330

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_09331

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032787 RA
      MFGWTLFLI IALTTVHIL NYIDHIIYY 
      SEETSHVLV IFFCAAIIT WVFLGALVL 
      ILLCSFVEQ EAEEIVSLS YDIRLSLTG 
      NVYKMQEFT NFVIKNLPK FTAANFFHI 
      GRSTILNML GTVSTFIII LIQFRKH
    CDS sequence
    • >TC032787 RA
      atgtttggc tggacattg ttcctaatc attgcttta 
      acaactgta catattctt aactacatc gaccacatt 
      atttactat tctgaagaa acaagtcac gtacttgtt 
      atttttttt tgtgcggca attattaca tgggttttt 
      ttaggagca cttgtcttg attttactc tgcagtttt 
      gtggaacag gaagccgaa gaaatagta tcgttgagt 
      tatgacata agattgagt ttgactgga aatgtttac 
      aaaatgcaa gaatttaca aatttcgtt attaagaat 
      ttgccaaaa ttcaccgca gcgaatttt ttccacatt 
      ggaaggtct acaatttta aacatgcta ggcacggtt 
      tccaccttc attatcatt ctgattcaa tttcggaag 
      cactaa
    mRNA sequence
    • >TC032787 RA
      tttttgtta aatgggggc acatactta gccatcgtc 
      cctccgtta aacaaacaa aataactac aagtataaa 
      ttttacgtt tacgcgatg tctgcattc atatctggc 
      agtgtgata gcaacattg ttcttcaaa gatttttac 
      gcagatttt gttcttata aaaaaagct gtgtgtata 
      gttgaagac attttcttg cttagtcta aacctacac 
      attttgaat attttcggt ttgcgtaga cgcgaacaa 
      tggagtgca ttaatcgag tgtttcaaa gccaccgaa 
      aatttagtt ttagtcgaa ttaaacaag cggaaagtc 
      ccgtattac ctgggattt gtcattgca aacattgtt 
      catttttcg attgcagtc tacgaatat tttttttgg 
      acacagtcc attggactt agttttata aaaaaatac 
      tttaacagg tacttgcaa atgtatttg aatttttat 
      tgcatattt ttactgtgt gtggttaca aacatgatt 
      ttgttacgg tataaaggg ttcaaagct acactaaga 
      acccaaact cacactcac ttaaacaaa aattcgctc 
      ctaaattta ataagcaaa gttgagaaa acaatgtat 
      tcattgaaa aaaatcgtc gatctttac aacaccatg 
      tttggttgg acgctattc ttgatcatt tctctgaca 
      acaatacaa attcttaac tacatcgat tttataatc 
      ttctacaca caagtggaa gcaggctct gtacaaatc 
      gtcctgctt tctttggca attattaca tgggttttt 
      tcaggagca cttgtctta attttactc tgcagcttc 
      gtggaacaa gaagtcgaa gaaattgta ctattgacg 
      tacgatatc aggctgaga gtggctgga aaaatcaac 
      ttagaaggc tacaaaatg caagagttt acgaacttc 
      gttaataaa aatttgcca agttttaca gctgcaaat 
      tttttccac attggaaga tcgactatt ttaaatatg 
      ttaagcaca gtttcgacg ttcattatt attctcatt 
      caatttcgc aaacaataa agtctcatt tattcacaa 
      atgctgtaa caataaaat tatttattc cttctctca 
      atttattta aataataag tttaatatt tcactggtg 
      atgtaataa caaaatatc taaataaaa cacgtatga 
      acaatgttt tcgttaccc tctgtcagc tttttattg 
      ccttgcatg tttcagggg ccgttattt atacttgac 
      acgagagat tatctaata aaaatataa cggttacaa 
      attacaaat aatgaacct acccagaac catttaaat 
      atcttggtt ttaaatcta atagaaaaa ttcctgcaa 
      tttaccact atttcagta gcacaatga gtattaaac 
      tgctgacac ttttgctaa aaggcgcca catatttag 
      ccatcgtcc cgcctttaa acaaccaaa atatccaca 
      agtataaat tttacgttt acgcgatgt ctgtattta 
      tatctgctg gtgtgatgg tcgctttgt tcttcaaga 
      atttttatt caggttttg ttctcataa agaaaattg 
      tttcgctgg ttgaagaca ctttcttgc tcagtctaa 
      atctgcaca ttctaaatg ttttcggtt tgcggaaac 
      gcaaacact ggaatgaat taatagcgt gtttcaaag 
      caaccgaaa atctaattt tcgtcaaag taaggcaga 
      ggaaagtcc cttattacc tgggatttg tcattgcaa 
      acatcacac attttgcga ttgcaatgt acgaatata 
      ttttttggg cccagtcac ttggactaa cttttctga 
      aagtctcct tggttggat acttacaat ggtatttaa 
      attgttatt gcatatttt tactgtgtg tggttacga 
      acatgatac tgtcacggt acaagggac tgaaatcga 
      ttataaagg cacattgca aatcacacc ctaatttaa 
      ataataatt caattttga atcttacaa gccaaattg 
      aaagaacga tgtgtttgt taaagagaa ctgtcgaca 
      tctacaaca ccatgtttg gctggacat tgttcctaa 
      tcattgctt taacaactg tacatattc ttaactaca 
      tcgaccaca ttatttact attctgaag aaacaagtc 
      acgtacttg ttatttttt tttgtgcgg caattatta 
      catgggttt ttttaggag cacttgtct tgattttac 
      tctgcagtt ttgtggaac aggaagccg aagaaatag 
      tatcgttga gttatgaca taagattga gtttgactg 
      gaaatgttt acaaaatgc aagaattta caaatttcg 
      ttattaaga atttgccaa aattcaccg cagcgaatt 
      ttttccaca ttggaaggt ctacaattt taaacatgc 
      taggcacgg tttccacct tcattatca ttctgattc 
      aatttcgga agcactaaa atctcaatt ataaacatt 
      tacagaatt ctgaatttt gtatgttaa cagttattt 
      tcgataaaa agtgtaatt gtgcaacca gatcagata 
      gaacaagag ttatgttcc ttgacataa ttgaagagt 
      tatcagatg tacccaatc gagaattat attaaaaat 
      atacttgtg ggaatttg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY