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Gene information
General information
TC016158 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 181298..187196
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC016158
      iBeetle screen iB_09325

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

      thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC016158 RA
        MELLGFVNL GEYTPEKDR DSLGDHALV LMFQPFAGK 
        SIQALGCFL SKGNVTGAV QAKLVLECI LHCENAGLY 
        IDAVTSDGA AWNRSMWKE FGIEDPLRP WCVHPVDKG 
        RLLRFASDW PHLIKCLRN LLISKKEFI LFSNSVALA 
        LRSYANEVP GLFDSEPTS KFCERVNKI IDIMNSSLP 
        SKALKYDSE DYKHFFGMM RAAGGCNNH AESLQIGQI 
        FRLMSLYSL VKPPRGSNI SGGEMLKTL ISVNDNENA 
        VLGIDKKTK FNLTFNHLS DDNESMPHV ETFNDNNEA 
        FAYVCGYLA KQYKKIECD EFSIVIIVR FGIAVWLHM 
        LVAALSASA MCPILGPCP RHGIRF
      CDS sequence
      • >TC016158 RA
        atggagtta ctgggcttt gtgaattta ggggaatac 
        actcccgaa aaagacaga gacagtttg ggagatcat 
        gccctagta ctgatgttt caacctttt gccggaaaa 
        tctattcaa gcactagga tgttttttg tcaaaggga 
        aatgttacg ggtgctgtt caagcgaaa ttagtgctt 
        gaatgtatt ctccattgc gaaaatgcc gggctctac 
        attgacgcg gtaacaagc gatggggct gcttggaat 
        cggtctatg tggaaagaa tttggaata gaggaccca 
        ttgcgtccg tggtgtgtt cacccagtt gacaaagga 
        cgattgctt agatttgct tcagactgg ccgcatttg 
        atcaaatgt ttacgcaat ttattaata tcaaaaaag 
        gagtttata ctttttagc aatagcgta gctctagct 
        ttaagatct tacgccaat gaagtacca ggtttattt 
        gattcggaa ccaacttca aaattctgt gaacgtgta 
        aataaaatt atagatatt atgaactct tctttaccc 
        agtaaagct ctgaaatat gattcagaa gattacaag 
        cacttcttt ggaatgatg cgcgcagct ggaggatgc 
        aataatcat gcagaatct ttacaaatc ggccaaatt 
        tttcgttta atgtcccta tattcactt gtaaaacct 
        cctcggggc agtaacata tcaggaggg gaaatgtta 
        aaaacttta ataagtgta aatgataat gaaaatgct 
        gtattagga atcgacaag aaaacaaaa ttcaactta 
        acatttaat catctatca gatgataac gaatcaatg 
        ccgcatgtt gaaactttt aatgataat aacgaagcc 
        tttgcttat gtttgtggt tatttggca aaacaatat 
        aaaaaaatc gaatgtgac gaattctcg atagtaata 
        attgtgcga ttcggcatc gcggtgtgg ttgcatatg 
        ctcgtggcc gccctgtcg gcctccgcc atgtgtccc 
        attctgggc ccgtgcccc cgacatgga atccgtttc 
        tag
      mRNA sequence
      • >TC016158 RA
        caaattata tgagcatat gagaagaga aaaacttat 
        gccattacc ttgccataa cacattatt aaattatat 
        aaaaaaaat tgacggcaa ttttggttt taaaacaaa 
        attatttga tgttttaaa agaaaaaac taaatttat 
        gaaagagtg ggaaaaaca cggatgtct tttattgga 
        tgaatgtaa aatttctcc atcaaagca ttttgacaa 
        gacaaaaat ggagttact gggctttgt gaatttagg 
        ggaatacac tcccgaaaa agacagaga cagtttggg 
        agatcatgc cctagtact gatgtttca accttttgc 
        cggaaaatc tattcaagc actaggatg ttttttgtc 
        aaagggaaa tgttacggg tgctgttca agcgaaatt 
        agtgcttga atgtattct ccattgcga aaatgccgg 
        gctctacat tgacgcggt aacaagcga tggggctgc 
        ttggaatcg gtctatgtg gaaagaatt tggaataga 
        ggacccatt gcgtccgtg gtgtgttca cccagttga 
        caaaggacg attgcttag atttgcttc agactggcc 
        gcatttgat caaatgttt acgcaattt attaatatc 
        aaaaaagga gtttatact ttttagcaa tagcgtagc 
        tctagcttt aagatctta cgccaatga agtaccagg 
        tttatttga ttcggaacc aacttcaaa attctgtga 
        acgtgtaaa taaaattat agatattat gaactcttc 
        tttacccag taaagctct gaaatatga ttcagaaga 
        ttacaagca cttctttgg aatgatgcg cgcagctgg 
        aggatgcaa taatcatgc agaatcttt acaaatcgg 
        ccaaatttt tcgtttaat gtccctata ttcacttgt 
        aaaacctcc tcggggcag taacatatc aggagggga 
        aatgttaaa aactttaat aagtgtaaa tgataatga 
        aaatgctgt attaggaat cgacaagaa aacaaaatt 
        caacttaac atttaatca tctatcaga tgataacga 
        atcaatgcc gcatgttga aacttttaa tgataataa 
        cgaagcctt tgcttatgt ttgtggtta tttggcaaa 
        acaatataa aaaaatcga atgtgacga attctcgat 
        agtaataat tgtgcgatt cggcatcgc ggtgtggtt 
        gcatatgct cgtggccgc cctgtcggc ctccgccat 
        gtgtcccat tctgggccc gtgcccccg acatggaat 
        ccgtttcta gatcagacc ccctaggtg gacccccca 
        aatgcacat tttgaaagt aaaaaaata tcaagcaaa 
        acaccccga ttttcaagg gtaaataaa aagcagtga 
        cccct
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY