Status
Please wait ...
Gene information
General information
TC011652 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 16016547..16045471
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC011652
    iBeetle screen iB_09320

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 4 number 2nd lateral oviduct: - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head musculature not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax musculature not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head capsule split - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax constricted - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC011652 RA
      MELENIVAN TVYLKAREG GSDSNKGKS KKWRKILQF 
      PHISQCIDL INKIDVRYE YVVDQQPIG KLLFEQWCE 
      VKRHEYHRC VKFLDIAKR YEVETDEQR LELANAIKK 
      NSWEFTNSM YFHRYLQWK WLEAQPVTY KTFRMYRVL 
      GKGGFGEVC ACQVRATGK MYACKKLEK KRIKKRKGE 
      AMVLIEKQI LQKINSRFV VNLAYAYET KDALCLVLT 
      IMNGGDLKF HIYNMGGEP GLDIARARF YAAEVVCGL 
      DHLHQQGIV YRDCKPENI LLDDAGHVR ISDLGLAVD 
      IPEGEFVRG RVGTVGYMA PEVIDNERY TFSPDWFSF 
      GCLLYEMIE GQAPFRARK EKVKREEVD RRVKSDQEK 
      YSSKFSEDA KSLCQQLLA KSPAQRLGG KAGRYGAAV 
      VKQHAFFQS LNWRRLEAG MVEPPFVPD PHAVYAKDV 
      LDIEQFSTV KGVNIDEND SSFYSKFNS GCVSIPWQN 
      EMIETDCFR QLNVFGANS TRSSDLILQ PVTTTENEG 
      CFPFRRKKK QLPRTKPIP INPSLLPQM TES
    CDS sequence
    • >TC011652 RA
      atggaattg gaaaatatc gtcgccaac acagtctat 
      ctaaaagcg agagagggt ggttccgat agtaataaa 
      ggcaagagc aaaaagtgg aggaagatt ctgcagttt 
      ccccatatc tcgcagtgt atagatctt atcaacaaa 
      atagatgtt cgctacgag tacgtggtg gaccagcag 
      cccatcgga aagctcttg ttcgagcag tggtgtgaa 
      gtgaagcgt catgaatac caccgctgt gtcaagttc 
      ctcgacatc gccaaacgc tatgaagtc gaaacggac 
      gaacaacgg ttggagttg gcaaacgcc atcaaaaag 
      aattcatgg gaattcact aattctatg tattttcat 
      cggtacctg cagtggaag tggctggaa gcgcaaccg 
      gtcacttac aagactttt aggatgtat cgcgtcttg 
      ggcaagggc ggattcgga gaggtctgc gcttgtcag 
      gtgcgagcg actggtaaa atgtacgcg tgtaaaaag 
      ctggaaaag aaacggata aagaaacgc aaaggcgaa 
      gcgatggtt ctgatcgag aagcagata ttgcaaaag 
      atcaattca cgatttgtt gtgaatctt gcctacgct 
      tacgaaacg aaagacgcc ctttgttta gtgttgacg 
      ataatgaat gggggcgat ttaaagttt cacatctac 
      aacatgggt ggagaaccg ggactggac attgcgagg 
      gcgcgattt tacgcagct gaagtcgtc tgtggactt 
      gatcatttg catcagcag ggcattgta tatagagac 
      tgtaaaccc gaaaacatt cttctcgac gatgcaggt 
      cacgtaagg atctcagat ttaggttta gccgtagac 
      atccctgaa ggagaattc gttagaggt agagtcggc 
      actgtaggc tacatggca cctgaagtt atagataac 
      gaacgttac acattcagt cccgattgg ttcagtttc 
      ggttgtttg ttgtacgaa atgattgaa ggccaggca 
      ccgttccga gcgcgtaaa gaaaaagtt aaacgtgaa 
      gaagtggat cgacgagtg aagtccgac caggagaaa 
      tactcgtcg aaattcagt gaagatgcc aaatctttg 
      tgccaacaa ttactagcc aaaagtccc gcacagaga 
      ctggggggc aaagcggga cgatatggg gctgctgta 
      gtcaaacaa cacgctttt tttcagagt cttaattgg 
      aggagactc gaggcaggg atggtcgaa ccccctttc 
      gtaccagat cctcatgcg gtttacgcc aaagacgtg 
      ctcgatatc gagcagttt tccaccgtc aaaggcgtg 
      aatattgac gagaacgat tcctcgttt tatagcaag 
      ttcaacagt ggttgtgtg tccatacca tggcaaaat 
      gaaatgata gaaacagac tgctttaga caattaaat 
      gtgtttggt gcgaacagt acgagaagc agcgattta 
      atacttcaa cccgtcacg acgacagaa aacgaaggt 
      tgctttcct ttccgacga aagaagaaa cagctgcct 
      aggaccaag cctatcccc ataaacccc tccttgtta 
      cctcaaatg acagagagc tga
    mRNA sequence
    • >TC011652 RA
      taagttgca atcaaacat catataact ggtcatgtg 
      accaaattc aacgaatcg gatgctcaa actcatgtg 
      tgataatta attgggcat taattttta acactgaat 
      cgtcaatta atttttaat tagactatt gttaaacca 
      gcttgtagt ttgtcttag ttgttttgc tcttccata 
      gaaaaaaat ctgcgagat ttttggttt ttcatttga 
      agatcgatc gtttggatc cccacccga gcgtcacca 
      ccgccgcat cgcctcctc gtgtgaacc tctccgtag 
      ttctgtttt cgtcctctt cgttgtggt gtcgctctg 
      ttcggtgga tagacgcgt gtcggtagc tgtgtgtac 
      ctagtaaag tcggtgaaa tttgttgag aaagtcgtc 
      tttgagtgc atcagggtg gcattccaa gggtcgcag 
      acacacccc gaagtggcg gcagcatcc ccttgggtg 
      cgcatcgag ggtgcaagt gcatcagcc cgaccccca 
      accaccccc ataattaat ggaaacggg tcgttcacg 
      acccgaccc cccgggtaa aaacaacca ccaaactgg 
      aactcatgg aattggaaa atatcgtcg ccaacacag 
      tctatctaa aagcgagag agggtggtt ccgatagta 
      ataaaggca agagcaaaa agtggagga agattctgc 
      agtttcccc atatctcgc agtgtatag atcttatca 
      acaaaatag atgttcgct acgagtacg tggtggacc 
      agcagccca tcggaaagc tcttgttcg agcagtggt 
      gtgaagtga agcgtcatg aataccacc gctgtgtca 
      agttcctcg acatcgcca aacgctatg aagtcgaaa 
      cggacgaac aacggttgg agttggcaa acgccatca 
      aaaagaatt catgggaat tcactaatt ctatgtatt 
      ttcatcggt acctgcagt ggaagtggc tggaagcgc 
      aaccggtca cttacaaga cttttagga tgtatcgcg 
      tcttgggca agggcggat tcggagagg tctgcgctt 
      gtcaggtgc gagcgactg gtaaaatgt acgcgtgta 
      aaaagctgg aaaagaaac ggataaaga aacgcaaag 
      gcgaagcga tggttctga tcgagaagc agatattgc 
      aaaagatca attcacgat ttgttgtga atcttgcct 
      acgcttacg aaacgaaag acgcccttt gtttagtgt 
      tgacgataa tgaatgggg gcgatttaa agtttcaca 
      tctacaaca tgggtggag aaccgggac tggacattg 
      cgagggcgc gattttacg cagctgaag tcgtctgtg 
      gacttgatc atttgcatc agcagggca ttgtatata 
      gagactgta aacccgaaa acattcttc tcgacgatg 
      caggtcacg taaggatct cagatttag gtttagccg 
      tagacatcc ctgaaggag aattcgtta gaggtagag 
      tcggcactg taggctaca tggcacctg aagttatag 
      ataacgaac gttacacat tcagtcccg attggttca 
      gtttcggtt gtttgttgt acgaaatga ttgaaggcc 
      aggcaccgt tccgagcgc gtaaagaaa aagttaaac 
      gtgaagaag tggatcgac gagtgaagt ccgaccagg 
      agaaatact cgtcgaaat tcagtgaag atgccaaat 
      ctttgtgcc aacaattac tagccaaaa gtcccgcac 
      agagactgg ggggcaaag cgggacgat atggggctg 
      ctgtagtca aacaacacg ctttttttc agagtctta 
      attggagga gactcgagg cagggatgg tcgaacccc 
      ctttcgtac cagatcctc atgcggttt acgccaaag 
      acgtgctcg atatcgagc agttttcca ccgtcaaag 
      gcgtgaata ttgacgaga acgattcct cgttttata 
      gcaagttca acagtggtt gtgtgtcca taccatggc 
      aaaatgaaa tgatagaaa cagactgct ttagacaat 
      taaatgtgt ttggtgcga acagtacga gaagcagcg 
      atttaatac ttcaacccg tcacgacga cagaaaacg 
      aaggttgct ttcctttcc gacgaaaga agaaacagc 
      tgcctagga ccaagccta tccccataa acccctcct 
      tgttacctc aaatgacag agagctgat gcctctaat 
      aatttaatt caatgtcaa cacactttc ctccccaac 
      gtctcatat tcgtcttcc ctgaaattt acgcacgac 
      gattgttac attttttgt atatattta agtgttgaa 
      agcatgcac aatgccatt ttggtagat gatatattt 
      tttataagt ttatttttt tgcgtgaga ttactttcg 
      ggagtcatc tactgatgt ggaaacggt tagttacac 
      agcttccgt gatagatag acagttaat taaaacgtc 
      tatatatca tgaaggctg tggttagtt gtctcaaaa 
      acgtatttg aatgttgtt ttgttttca ttccacaat 
      cacgtcacc taatggatt agataattg atgtatttc 
      aagaaggaa gaaaaaaga ccttttata ttattttat 
      tgtgatatt ttatctgtg tgtttgtgt ctaccagga 
      cttgatttc aacggccca ataataaag ttcaaaaca 
      gattttaac ttgcccaag aatttgtct acattaagc 
      tcaaatatt aggatagaa atccattaa ctttcaatt 
      gtatgtata agcagtttg tattgtttt tttatttaa 
      tttatacat tttttgagc gactgtttt ttgtaaatt 
      cgttggtcc gtcactgtg agatcatta aacagagtg 
      aatttaaaa aaaaacgtc caaacgttt gtatgtata 
      ctccaggaa acaattcct gactcacgt tgactgtcc 
      aaccaaact tttttttaa attatgcct gttttgact 
      ttgtattga aatgatttt tgataatta attaaacat 
      ctaaatcat aa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY