Status
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Gene information
General information
TC011217 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 1822518..1825309
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC011217
      Gene ontology

      GO terms for Tribolium

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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
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      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
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      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
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      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
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      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_09319

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC011217 RA
        MEERGQRAY ACKFPLCNS VAYRKDRNK RFFKSPSDQ 
        GIQQLWKTI CNIEPGVRC ENFRICEDH FRVSDFRGS 
        RTLCPFAVP KAVFNDRVN DEHSYCGTD EGISEVEIE 
        DNVLPSCDL KVLECDVSE ELPSLAKPD LAVNEQCQN 
        FESVEEVLP VSNIITEDN QPSCSFAAT EARGIRKRW 
        KQPIAYYFT ASTISAVAL KQIIVKAVE KLKSLGLIV 
        LATVCDQGA TNRSAMTQL CSSVNDTPL GYHFEVDGS 
        KINCIFDPV HLLKNTRNA LIENFIEFA PGKRADFEH 
        ILMVFESDQ KKKFRSLHK LSREDFNFK NSYTKMKVS 
        VAARQLSYS VACELETYV SHNQLPPNA VHTAEFVHL 
        IDQLFDSCN GTTKYSKRE SKPLRCAIR EDSPHINFW 
        TDLLAKINN WKIINKATG KNVTNQFHF INGWKVTIR 
        SIIAIWHDL QNLGLEYLC GRVLNQDTV ENLFCCVRQ 
        HGIANTNPT CFQFMSALK TYVLNNLVM PRNSQSNCK 
        DDGDSLLDN LMALLKEGV QDDSVSATD VVFEDESLE 
        HQDFFTNVN VEDIQGLAY VSGYIVKKM AVPDCAPCK 
        DALHSPDVT PAHTTIMFR DDGKRLVYA PESFINFVN 
        GTNEPLCLF LKTCATKNF LEDQFKGYC MSKDTFQNF 
        FTCSSHSAQ MLDEFFKVS IPFLIFKYV NEINKGSQH 
        AATRALKKH VSKIQKFTS
      CDS sequence
      • >TC011217 RA
        atggaagaa agaggtcaa agggcgtat gcttgcaaa 
        ttccccctg tgtaatagc gttgcttac cgtaaggac 
        cgcaataag aggtttttc aaatctcct agtgatcaa 
        ggaatacag cagctgtgg aaaacaatt tgtaacatc 
        gaacctggc gttcgatgt gaaaatttt cgtatttgt 
        gaagatcac tttcgagtt tccgatttt agaggtagt 
        cgcacactg tgccctttt gccgttcca aaagctgtc 
        ttcaatgat cgggttaat gacgaacat tcttattgt 
        ggaactgat gaaggaatt tcagaagta gaaattgaa 
        gacaacgta ctaccctcc tgtgattta aaagttctt 
        gaatgtgat gtaagtgaa gaacttcct tcattagcg 
        aagccggat ttagcagtg aatgaacag tgtcaaaat 
        tttgaatct gtagaagaa gttttgcca gtttcaaac 
        atcattacc gaagataat caaccaagt tgttcattc 
        gctgctaca gaagcaaga ggtattcgt aaaagatgg 
        aagcagccc atcgcgtat tattttacc gcttccaca 
        atttcggca gttgcgttg aagcaaatt attgttaaa 
        gccgtagaa aaattaaaa tcattagga ctgattgtt 
        cttgctaca gtctgtgac caaggtgct accaataga 
        tcagctatg acacagttg tgttcttcg gtaaatgac 
        actccttta ggttatcat tttgaagtc gacggttcc 
        aaaattaat tgcattttt gatccagta catttattg 
        aagaacact aggaacgca ctaattgaa aattttatt 
        gaattcgcg ccaggcaag agagcagat tttgagcac 
        atcttaatg gtttttgaa tcagatcag aaaaagaaa 
        tttagatca ttgcataaa ttatctcga gaagatttt 
        aatttcaaa aattcttac acaaaaatg aaagtttct 
        gttgctgcg cgtcagttg agttacagt gttgcctgt 
        gagttagaa acgtatgtc tcacataat caattacca 
        ccaaatgca gttcacacc gcggaattt gtccattta 
        attgatcaa ctttttgat tcttgtaat ggcaccaca 
        aaatattct aaacgcgag agcaaacca ttgagatgt 
        gcaattaga gaagattcc ccacatatt aatttttgg 
        acggacctt ttggcaaaa ataaataat tggaaaata 
        ataaataaa gcaactggt aagaatgtt actaaccaa 
        tttcatttc attaatgga tggaaagtg acaattcgt 
        tccataatt gcaatttgg catgatctt caaaatttg 
        gggttggag tatttgtgt ggtcgtgtt ttaaatcaa 
        gatacggtt gaaaatttg ttctgctgt gttcgacag 
        catggcatt gcaaatacc aaccccacg tgttttcaa 
        tttatgtct gctttgaag acgtatgtt cttaacaat 
        ctagttatg ccacgcaac tctcaaagc aactgtaaa 
        gatgatggg gattcttta cttgacaat ttgatggct 
        cttctaaaa gaaggtgtt caagatgac tctgtttcc 
        gctacggat gtagtgttt gaggatgag tcgcttgaa 
        caccaagac ttttttacc aatgtaaac gtggaggac 
        atccaggga ttggcctat gtgtcaggt tatattgtc 
        aagaaaatg gcagtcccc gattgtgct ccatgtaaa 
        gacgctctt cattcccca gacgtgact cctgcacac 
        acaactata atgttccgt gatgatggt aaacgtctt 
        gtttacgca cctgagagt tttattaac tttgttaac 
        ggaactaat gaacctctg tgtttgttt ttgaaaaca 
        tgtgcgacc aaaaatttt ttagaagat cagtttaag 
        ggttattgt atgtcgaaa gacactttt caaaatttt 
        tttacttgt tccagtcac agtgctcag atgttggat 
        gagtttttc aaggtttca atccccttt ttaatcttc 
        aaatatgtt aatgaaatt aacaaagga tcgcaacat 
        gctgcaact cgcgctttg aaaaaacat gtttcaaag 
        atccagaag ttcacaagt tga
      mRNA sequence
      • >TC011217 RA
        atggaagaa agaggtcaa agggcgtat gcttgcaaa 
        ttccccctg tgtaatagc gttgcttac cgtaaggac 
        cgcaataag aggtttttc aaatctcct agtgatcaa 
        ggaatacag cagctgtgg aaaacaatt tgtaacatc 
        gaacctggc gttcgatgt gaaaatttt cgtatttgt 
        gaagatcac tttcgagtt tccgatttt agaggtagt 
        cgcacactg tgccctttt gccgttcca aaagctgtc 
        ttcaatgat cgggttaat gacgaacat tcttattgt 
        ggaactgat gaaggaatt tcagaagta gaaattgaa 
        gacaacgta ctaccctcc tgtgattta aaagttctt 
        gaatgtgat gtaagtgaa gaacttcct tcattagcg 
        aagccggat ttagcagtg aatgaacag tgtcaaaat 
        tttgaatct gtagaagaa gttttgcca gtttcaaac 
        atcattacc gaagataat caaccaagt tgttcattc 
        gctgctaca gaagcaaga ggtattcgt aaaagatgg 
        aagcagccc atcgcgtat tattttacc gcttccaca 
        atttcggca gttgcgttg aagcaaatt attgttaaa 
        gccgtagaa aaattaaaa tcattagga ctgattgtt 
        cttgctaca gtctgtgac caaggtgct accaataga 
        tcagctatg acacagttg tgttcttcg gtaaatgac 
        actccttta ggttatcat tttgaagtc gacggttcc 
        aaaattaat tgcattttt gatccagta catttattg 
        aagaacact aggaacgca ctaattgaa aattttatt 
        gaattcgcg ccaggcaag agagcagat tttgagcac 
        atcttaatg gtttttgaa tcagatcag aaaaagaaa 
        tttagatca ttgcataaa ttatctcga gaagatttt 
        aatttcaaa aattcttac acaaaaatg aaagtttct 
        gttgctgcg cgtcagttg agttacagt gttgcctgt 
        gagttagaa acgtatgtc tcacataat caattacca 
        ccaaatgca gttcacacc gcggaattt gtccattta 
        attgatcaa ctttttgat tcttgtaat ggcaccaca 
        aaatattct aaacgcgag agcaaacca ttgagatgt 
        gcaattaga gaagattcc ccacatatt aatttttgg 
        acggacctt ttggcaaaa ataaataat tggaaaata 
        ataaataaa gcaactggt aagaatgtt actaaccaa 
        tttcatttc attaatgga tggaaagtg acaattcgt 
        tccataatt gcaatttgg catgatctt caaaatttg 
        gggttggag tatttgtgt ggtcgtgtt ttaaatcaa 
        gatacggtt gaaaatttg ttctgctgt gttcgacag 
        catggcatt gcaaatacc aaccccacg tgttttcaa 
        tttatgtct gctttgaag acgtatgtt cttaacaat 
        ctagttatg ccacgcaac tctcaaagc aactgtaaa 
        gatgatggg gattcttta cttgacaat ttgatggct 
        cttctaaaa gaaggtgtt caagatgac tctgtttcc 
        gctacggat gtagtgttt gaggatgag tcgcttgaa 
        caccaagac ttttttacc aatgtaaac gtggaggac 
        atccaggga ttggcctat gtgtcaggt tatattgtc 
        aagaaaatg gcagtcccc gattgtgct ccatgtaaa 
        gacgctctt cattcccca gacgtgact cctgcacac 
        acaactata atgttccgt gatgatggt aaacgtctt 
        gtttacgca cctgagagt tttattaac tttgttaac 
        ggaactaat gaacctctg tgtttgttt ttgaaaaca 
        tgtgcgacc aaaaatttt ttagaagat cagtttaag 
        ggttattgt atgtcgaaa gacactttt caaaatttt 
        tttacttgt tccagtcac agtgctcag atgttggat 
        gagtttttc aaggtttca atccccttt ttaatcttc 
        aaatatgtt aatgaaatt aacaaagga tcgcaacat 
        gctgcaact cgcgctttg aaaaaacat gtttcaaag 
        atccagaag ttcacaagt tga
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY