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Gene information
General information
TC009986 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007422.5: 10694741..10697769
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC009986
    iBeetle screen iB_09318

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC009986 RA
      MKSLFGIFV LYQIFSIGA TFGDHILIT KYCEAKNIH 
      IGTIFSCQN KRDNMKKII SLSTVGLRF LNVDISHLH 
      NQNFPLDQL DQVLNRNHY HLAVIIDSG CIDYADFAI 
      QDKKYFYET YHWLVPTTP QNLNNSLNF LQKSPLNIN 
      SDVNVAILN GEGTKWSIL DVYNPASSH HGQFTVTKL 
      GLCDETNGY QAKIAGNKY WSRKNMTGV QFKSAVVVP 
      DPSIKLNDY LTSDKNRQL HSMHRFQSV TVNYCREMY 
      NFSLEIQRT NSWGYLTPN GHFDGLVGL LERRLVDFG 
      SSPLIYKLD RMPVIDYSY GNWVLRSTF IYRRPKIIE 
      ASYKIFLRP LSRTVWICI VLMMVLLML FLKVVFSRE 
      KRLLQKRNL VDSSWSFLF LFTLGAFCQ QGATCHPQL 
      LSSRTLSIF VFLFCILTY QFYSASIVS YLLIDPPRK 
      INNLKDLSD SNLRAGIED ILIDRNYFV QTTDPVAIE 
      LFNKKIKFS NNNSGFYEP WDGLDLVKQ GGFAFHVET 
      STAYPIIEE TFTNEEICE LEEVQMYRT QPMHTNLQK 
      NSPFREMMN YCMLHLVEN GLMYRLRKY WDARKPMCI 
      ESAKKFTFN VGLKEFSSG LIVLSYGIL ISLGLLLRE 
      VIVHKKVYI KDLFRKKNA KNAVQPFTN
    CDS sequence
    • >TC009986 RA
      atgaaaagt ctctttggt atttttgta ttgtaccaa 
      atattctca ataggcgca acgtttggt gatcacatt 
      ttgatcaca aagtattgc gaagcaaag aacattcat 
      attgggacc attttctca tgccaaaat aaacgagat 
      aatatgaaa aaaataatt tcattaagt acagtagga 
      ttacgcttt ttaaatgtt gatatcagc caccttcat 
      aaccaaaat tttccgtta gatcaactg gatcaagtg 
      ctaaatcgt aaccattat caccttgct gttataata 
      gacagcggt tgtattgac tatgctgat tttgccatc 
      caggacaaa aaatatttt tacgaaact tatcattgg 
      ctggttcct acaacgcca caaaatctc aacaactcc 
      ctaaacttt ctacaaaaa tcaccacta aacataaat 
      tcagacgta aacgttgca attttaaac ggcgaaggc 
      actaagtgg tcaattttg gacgtgtat aaccctgcc 
      tcaagccac cacggccaa tttacagtc acaaaatta 
      ggactatgt gatgaaaca aacggctat caagcaaaa 
      atcgccggt aacaaatac tggtcaaga aaaaacatg 
      acaggggtt caattcaaa tcagctgtc gtggtccct 
      gaccctagc attaaacta aatgactat ctaacaagt 
      gacaaaaat cgccaactc cactccatg catagattt 
      caaagcgta acggttaac tactgccga gaaatgtac 
      aacttcagt ttggaaatt cagagaact aattcgtgg 
      ggttatctg accccgaac ggccatttc gacggcttg 
      gtggggctg cttgagcgc agacttgtg gattttggg 
      agctctccg ttgatttac aagcttgac cgaatgccg 
      gtcattgat tacagttac ggaaattgg gttttgcgg 
      tctactttt atctacaga cgcccgaaa atcattgag 
      gcgtcttac aagattttc ttgcggcct ttatcacga 
      accgtttgg atttgtatc gttttaatg atggtgctt 
      ttgatgctt tttttgaag gtggttttc agtcgcgag 
      aagcgcctt ttgcaaaag cgcaatttg gttgacagc 
      agttggagc ttcctgttt ttgttcacc ttgggggcg 
      ttttgccag cagggggcc acctgccac ccccagtta 
      ctgagcagt cggacgtta tccattttt gtttttctt 
      ttttgcata ttgacttat cagttttac tcggccagt 
      attgtgtct tatttattg atagacccg cctcggaaa 
      ataaataat ttaaaagat ttgtctgac agtaatttg 
      agagccgga attgaggat attttgatt gacagaaat 
      tactttgtg caaactaca gatccggta gctatcgaa 
      ttatttaat aaaaaaatc aagtttagt aacaacaat 
      tcgggcttt tatgaaccg tgggatggg ctagatttg 
      gtgaaacag ggcgggttt gcgtttcat gttgaaacc 
      agcactgcg taccccatt atcgaggaa actttcaca 
      aatgaggaa atttgcgag cttgaagaa gtgcaaatg 
      tataggacg cagcccatg catactaat cttcagaag 
      aactcgccg tttcgcgaa atgatgaac tactgtatg 
      ttgcatttg gtggaaaac ggactaatg tatcgctta 
      cgaaaatac tgggacgct cgcaagcca atgtgcatc 
      gagtctgcc aaaaaattc acatttaat gttggtctc 
      aaggagttt tcttcaggc ctgattgtt ctttcttac 
      ggtatttta atttcactg ggtttgttg ttaagggag 
      gtgatagtt cacaaaaaa gtgtacatt aaagatttg 
      tttcgtaag aaaaatgcg aaaaatgcc gttcaacca 
      ttcaccaat tga
    mRNA sequence
    • >TC009986 RA
      atgaaaagt ctctttggt atttttgta ttgtaccaa 
      atattctca ataggcgca acgtttggt gatcacatt 
      ttgatcaca aagtattgc gaagcaaag aacattcat 
      attgggacc attttctca tgccaaaat aaacgagat 
      aatatgaaa aaaataatt tcattaagt acagtagga 
      ttacgcttt ttaaatgtt gatatcagc caccttcat 
      aaccaaaat tttccgtta gatcaactg gatcaagtg 
      ctaaatcgt aaccattat caccttgct gttataata 
      gacagcggt tgtattgac tatgctgat tttgccatc 
      caggacaaa aaatatttt tacgaaact tatcattgg 
      ctggttcct acaacgcca caaaatctc aacaactcc 
      ctaaacttt ctacaaaaa tcaccacta aacataaat 
      tcagacgta aacgttgca attttaaac ggcgaaggc 
      actaagtgg tcaattttg gacgtgtat aaccctgcc 
      tcaagccac cacggccaa tttacagtc acaaaatta 
      ggactatgt gatgaaaca aacggctat caagcaaaa 
      atcgccggt aacaaatac tggtcaaga aaaaacatg 
      acaggggtt caattcaaa tcagctgtc gtggtccct 
      gaccctagc attaaacta aatgactat ctaacaagt 
      gacaaaaat cgccaactc cactccatg catagattt 
      caaagcgta acggttaac tactgccga gaaatgtac 
      aacttcagt ttggaaatt cagagaact aattcgtgg 
      ggttatctg accccgaac ggccatttc gacggcttg 
      gtggggctg cttgagcgc agacttgtg gattttggg 
      agctctccg ttgatttac aagcttgac cgaatgccg 
      gtcattgat tacagttac ggaaattgg gttttgcgg 
      tctactttt atctacaga cgcccgaaa atcattgag 
      gcgtcttac aagattttc ttgcggcct ttatcacga 
      accgtttgg atttgtatc gttttaatg atggtgctt 
      ttgatgctt tttttgaag gtggttttc agtcgcgag 
      aagcgcctt ttgcaaaag cgcaatttg gttgacagc 
      agttggagc ttcctgttt ttgttcacc ttgggggcg 
      ttttgccag cagggggcc acctgccac ccccagtta 
      ctgagcagt cggacgtta tccattttt gtttttctt 
      ttttgcata ttgacttat cagttttac tcggccagt 
      attgtgtct tatttattg atagacccg cctcggaaa 
      ataaataat ttaaaagat ttgtctgac agtaatttg 
      agagccgga attgaggat attttgatt gacagaaat 
      tactttgtg caaactaca gatccggta gctatcgaa 
      ttatttaat aaaaaaatc aagtttagt aacaacaat 
      tcgggcttt tatgaaccg tgggatggg ctagatttg 
      gtgaaacag ggcgggttt gcgtttcat gttgaaacc 
      agcactgcg taccccatt atcgaggaa actttcaca 
      aatgaggaa atttgcgag cttgaagaa gtgcaaatg 
      tataggacg cagcccatg catactaat cttcagaag 
      aactcgccg tttcgcgaa atgatgaac tactgtatg 
      ttgcatttg gtggaaaac ggactaatg tatcgctta 
      cgaaaatac tgggacgct cgcaagcca atgtgcatc 
      gagtctgcc aaaaaattc acatttaat gttggtctc 
      aaggagttt tcttcaggc ctgattgtt ctttcttac 
      ggtatttta atttcactg ggtttgttg ttaagggag 
      gtgatagtt cacaaaaaa gtgtacatt aaagatttg 
      tttcgtaag aaaaatgcg aaaaatgcc gttcaacca 
      ttcaccaat tga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY