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Gene information
General information
TC032844
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC008210
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 6731744..6735920
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032844
    iBeetle screen iB_09315

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032844 RA
      MLSYKKITI ISACCFVVT IIFGFVAYE PIFEYAIRD 
      QISLHRRNF VRQFYLKYP IPLDFRVNF FNVSNPDEV 
      ENGGVPVLS EVGPYCYDL YKERIDVED NEAEDSLTY 
      TPYDIYLFN QERSGNLSQ DDYVTIIHP LVVFLNDAL 
      GFLFPEKSI FLTAKVRDI LFDGMLINC TSRDFTAMA 
      VCTQIRTKI PGIQFESKD YLKYALLGQ QNGTLPTRI 
      TVLRGIKES ENLGKLVAV DNVTKSDFW SNEECNEYK 
      GTDGWIFPP FSGRLKTIW MHATTLCQN IHADFVGPA 
      TSNGFAVNK YYSDFQNIC TNCSLQEPC LPEGLIDVT 
      KCLTAPIYI SLPHFLRSD ESLIRGVKG LNPDTESHI 
      TRILLEGTL SLPMEAQIR LQFNFPVQP VKKITIMQN 
      VSEVIHPVL WVEMGVVLN GWFLRMIKT FFYFLTALE 
      VMKYISLVA SLLGTAYGG YHLYKNKKL YSFKENIQT 
      ALRRRNIMK KVYLKIPMP LDFRVYFFN ITNPSEVQN 
      GELPVVKEV GPYCYDAFK EKIDVLENE GEDSLTYTP 
      YETYFFNQD KSGRLTADD YVTVLHPLI VGIVNTVSR 
      DSPPLLPIV DRAIKSIFK DPQNIYITT KVRDFLFDG 
      MTINCKVQD FSATAVCTQ LKAQIPGLI EIEKNVYKF 
      SILGPRNGT LPNRYKVFR GMKKWHELG RLVEVNHEK 
      ESTVWSTKK CNRFRGTDG WIFPPFIDK EVGFWTYSS 
      DLCRNMHLV FVEETSFHG VPAEKYYAD LGDMSSNPD 
      EKCYCPKTC LPKGMMDLT RCMGVPIYA TLPHFLRVD 
      KEVRRTVRG LKPITDEHI VRVIIQPLL GTPLEAQKR 
      MQFNLPIQP VKKISLMKT LPQALHPIF WIEEAIVLE 
      GPLFKMIKV VFVALKVFD VVKYCLLAV CLAFVAFGS 
      YLCYKERKQ KKQAVTPVS KTASTEHLL KSEQNAFEE 
      NKKIEDYIK NDEKIGKF
    CDS sequence
    • >TC032844 RA
      atgctttct tacaagaag ataactatt atatcagca 
      tgttgcttt gttgtgacg atcattttt gggttcgtt 
      gcttacgaa ccaattttc gaatatgca attagagac 
      caaatttct ctgcatcgg aggaacttt gtgagacag 
      ttttaccta aaataccca atacctttg gacttccgt 
      gtcaatttt ttcaacgtt tcaaaccct gacgaagta 
      gaaaacggt ggagtccct gttttgagt gaagtcgga 
      ccttactgc tatgatctt tacaaggag agaatagac 
      gtggaggac aacgaggcc gaggacagc ctcacctac 
      accccttac gacatttac ttatttaat caggagaga 
      tcgggaaac ttaagtcag gacgattac gttacaatt 
      attcacccc cttgtcgtg tttctaaac gacgcactt 
      ggatttctg ttccctgaa aaatccatc tttcttacc 
      gccaaagtc agagatatt ctatttgac ggaatgtta 
      ataaactgc acgtctcgg gatttcaca gcgatggca 
      gtttgtaca caaatacga acaaaaatt cccggaata 
      cagtttgaa agtaaagat tatttgaaa tatgcgctc 
      cttggacaa caaaatggg accttacct acacgtata 
      acggttttg agaggcata aaagagtca gagaatttg 
      ggcaaattg gtggctgtt gataacgtg acaaaaagt 
      gatttctgg tccaatgag gaatgtaat gagtataag 
      ggcaccgat ggttggatc ttccccccg ttttctggg 
      aggttgaag acgatctgg atgcacgca acaacactg 
      tgccaaaat atccatgcc gatttcgtt ggtcctgct 
      acaagtaac ggttttgca gtaaataaa tactactct 
      gactttcaa aatatttgt acgaattgc tcgcttcaa 
      gagccttgc cttccggaa ggattaatc gacgtcacg 
      aaatgtcta acagctcct atttacatc agtttaccc 
      cactttttg cgaagcgat gaaagtctg attcgcggg 
      gtcaaaggc ttgaatcct gatactgaa agtcacatc 
      acgagaatt ctcctcgag gggacactg tcgcttccg 
      atggaggcc cagattcgt ctccagttc aattttcct 
      gtccaacca gtcaagaaa ataactatt atgcaaaac 
      gtgtctgaa gtaattcat ccggtttta tgggttgaa 
      atgggagtg gttctgaac ggatggttt ctccgaatg 
      attaaaact ttcttttac ttcctcaca gctttagaa 
      gttatgaaa tacatctcg ctggtcgca agtctcctt 
      ggaacggca tacggaggc taccacttg tacaaaaac 
      aagaaactt tactcgttc aaggaaaat attcaaact 
      gcgcttagg cggagaaat ataatgaag aaggtgtac 
      ttgaaaatc cccatgccg ttggatttt cgcgtctat 
      tttttcaac atcacgaat ccgtctgaa gtgcaaaac 
      ggcgaactg ccggtggtt aaagaagtc ggaccgtac 
      tgttacgat gcctttaag gaaaaaatc gacgttttg 
      gagaacgag ggcgaggac agtctcact tacaccccc 
      tacgaaact tattttttc aatcaggac aaatccggg 
      cgcttgact gccgacgat tacgtgacg gttctccat 
      cccctgatt gtgggtatt gttaatacc gtttcaaga 
      gattcaccg cctttgtta cccatcgtg gaccgagcc 
      attaaatca atattcaaa gacccgcaa aatatctac 
      atcacaacg aaagttcgg gactttttg tttgacgga 
      atgacgatt aactgcaaa gttcaggac ttttcggca 
      acggctgtt tgcacgcaa cttaaagcc cagattccg 
      ggattgatt gaaattgag aaaaatgtt tacaagttt 
      tcgattcta ggaccgcga aatggcacg ttaccgaac 
      cggtataaa gtcttcaga gggatgaaa aagtggcac 
      gaattgggt cgtttagtt gaggtgaat cacgaaaag 
      gagtcaaca gtgtggtcc acgaaaaaa tgtaacaga 
      tttagggga actgacggt tggattttc cccccgttt 
      atcgacaag gaagtgggt ttttggacg tattcttcc 
      gatttgtgc cgcaatatg cacctcgtc ttcgtcgaa 
      gagacgagt tttcacgga gttcccgct gagaaatat 
      tacgccgat ttgggcgac atgtcttca aaccctgac 
      gagaagtgt tactgtccg aagacttgt ctcccgaaa 
      ggcatgatg gacttgacc cgctgtatg ggggtgccc 
      atctacgcc accctgccg cacttcctc cgagtggac 
      aaagaagtg aggagaact gtcagaggt ttgaagcca 
      atcaccgac gaacacatc gtgcgagtg ataatccaa 
      cctttgttg ggaactcca ctagaagca caaaagaga 
      atgcaattc aatttgccg atccaacct gtcaagaaa 
      attagttta atgaaaacg ttgcctcag gccctacat 
      cccattttc tggatcgag gaagcgatc gttttggaa 
      ggaccgctg tttaaaatg attaaagtc gttttcgtt 
      gctttgaaa gtgtttgat gttgttaag tattgcttg 
      ttggcggtt tgtctcgcg tttgtggct tttgggagc 
      tacttgtgc tacaaggag aggaagcag aagaaacag 
      gcggtgacg cccgtgtcc aaaactgcc agtactgag 
      catttactt aagtctgaa caaaacgct tttgaagaa 
      aataaaaaa attgaagac tatattaaa aatgacgaa 
      aaaattgga aaattttaa
    mRNA sequence
    • >TC032844 RA
      ttctgcaag aattattgt tagtgcaat atgctttct 
      tacaagaag ataactatt atatcagca tgttgcttt 
      gttgtgacg atcattttt gggttcgtt gcttacgaa 
      ccaattttc gaatatgca attagagac caaatttct 
      ctgcatcgg aggaacttt gtgagacag ttttaccta 
      aaataccca atacctttg gacttccgt gtcaatttt 
      ttcaacgtt tcaaaccct gacgaagta gaaaacggt 
      ggagtccct gttttgagt gaagtcgga ccttactgc 
      tatgatctt tacaaggag agaatagac gtggaggac 
      aacgaggcc gaggacagc ctcacctac accccttac 
      gacatttac ttatttaat caggagaga tcgggaaac 
      ttaagtcag gacgattac gttacaatt attcacccc 
      cttgtcgtg tttctaaac gacgcactt ggatttctg 
      ttccctgaa aaatccatc tttcttacc gccaaagtc 
      agagatatt ctatttgac ggaatgtta ataaactgc 
      acgtctcgg gatttcaca gcgatggca gtttgtaca 
      caaatacga acaaaaatt cccggaata cagtttgaa 
      agtaaagat tatttgaaa tatgcgctc cttggacaa 
      caaaatggg accttacct acacgtata acggttttg 
      agaggcata aaagagtca gagaatttg ggcaaattg 
      gtggctgtt gataacgtg acaaaaagt gatttctgg 
      tccaatgag gaatgtaat gagtataag ggcaccgat 
      ggttggatc ttccccccg ttttctggg aggttgaag 
      acgatctgg atgcacgca acaacactg tgccaaaat 
      atccatgcc gatttcgtt ggtcctgct acaagtaac 
      ggttttgca gtaaataaa tactactct gactttcaa 
      aatatttgt acgaattgc tcgcttcaa gagccttgc 
      cttccggaa ggattaatc gacgtcacg aaatgtcta 
      acagctcct atttacatc agtttaccc cactttttg 
      cgaagcgat gaaagtctg attcgcggg gtcaaaggc 
      ttgaatcct gatactgaa agtcacatc acgagaatt 
      ctcctcgag gggacactg tcgcttccg atggaggcc 
      cagattcgt ctccagttc aattttcct gtccaacca 
      gtcaagaaa ataactatt atgcaaaac gtgtctgaa 
      gtaattcat ccggtttta tgggttgaa atgggagtg 
      gttctgaac ggatggttt ctccgaatg attaaaact 
      ttcttttac ttcctcaca gctttagaa gttatgaaa 
      tacatctcg ctggtcgca agtctcctt ggaacggca 
      tacggaggc taccacttg tacaaaaac aagaaactt 
      tactcgttc aaggaaaat attcaaact gcgcttagg 
      cggagaaat ataatgaag aaggtgtac ttgaaaatc 
      cccatgccg ttggatttt cgcgtctat tttttcaac 
      atcacgaat ccgtctgaa gtgcaaaac ggcgaactg 
      ccggtggtt aaagaagtc ggaccgtac tgttacgat 
      gcctttaag gaaaaaatc gacgttttg gagaacgag 
      ggcgaggac agtctcact tacaccccc tacgaaact 
      tattttttc aatcaggac aaatccggg cgcttgact 
      gccgacgat tacgtgacg gttctccat cccctgatt 
      gtgggtatt gttaatacc gtttcaaga gattcaccg 
      cctttgtta cccatcgtg gaccgagcc attaaatca 
      atattcaaa gacccgcaa aatatctac atcacaacg 
      aaagttcgg gactttttg tttgacgga atgacgatt 
      aactgcaaa gttcaggac ttttcggca acggctgtt 
      tgcacgcaa cttaaagcc cagattccg ggattgatt 
      gaaattgag aaaaatgtt tacaagttt tcgattcta 
      ggaccgcga aatggcacg ttaccgaac cggtataaa 
      gtcttcaga gggatgaaa aagtggcac gaattgggt 
      cgtttagtt gaggtgaat cacgaaaag gagtcaaca 
      gtgtggtcc acgaaaaaa tgtaacaga tttagggga 
      actgacggt tggattttc cccccgttt atcgacaag 
      gaagtgggt ttttggacg tattcttcc gatttgtgc 
      cgcaatatg cacctcgtc ttcgtcgaa gagacgagt 
      tttcacgga gttcccgct gagaaatat tacgccgat 
      ttgggcgac atgtcttca aaccctgac gagaagtgt 
      tactgtccg aagacttgt ctcccgaaa ggcatgatg 
      gacttgacc cgctgtatg ggggtgccc atctacgcc 
      accctgccg cacttcctc cgagtggac aaagaagtg 
      aggagaact gtcagaggt ttgaagcca atcaccgac 
      gaacacatc gtgcgagtg ataatccaa cctttgttg 
      ggaactcca ctagaagca caaaagaga atgcaattc 
      aatttgccg atccaacct gtcaagaaa attagttta 
      atgaaaacg ttgcctcag gccctacat cccattttc 
      tggatcgag gaagcgatc gttttggaa ggaccgctg 
      tttaaaatg attaaagtc gttttcgtt gctttgaaa 
      gtgtttgat gttgttaag tattgcttg ttggcggtt 
      tgtctcgcg tttgtggct tttgggagc tacttgtgc 
      tacaaggag aggaagcag aagaaacag gcggtgacg 
      cccgtgtcc aaaactgcc agtactgag catttactt 
      aagtctgaa caaaacgct tttgaagaa aataaaaaa 
      attgaagac tatattaaa aatgacgaa aaaattgga 
      aaattttaa cgtaatttc aacacactt agtagaatc 
      actggaact tcatcaaag agcacaaaa ttactcaac 
      aactgccta ttatacata aataaattt tttggttac 
      at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY