Status
Please wait ...
Gene information
General information
TC007823 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007419.2: 1045237..1046661
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC007823
    iBeetle screen iB_09313

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC007823 RA
      MEQQRAIIE TSLTFFKVL GIHPLKTLR KIPVLCLIS 
      SHLILFALV CLRFFYEKI TFGLISDTF ESTFTIVHV 
      LAKFITLVI IKPTIKKLF ETTNTFWLG KTFQKEFRH 
      EVDADLRTL GLLLRLFLF FVSIVIVTF AIRPIFDDT 
      LAIHCYVPE FIPRAIFIV FNNAVFAVS AFAVGSFDM 
      FVCVMVVLI TVQFKILNY SILGLDLGK VVTENDEKL 
      CQKKIKIIV DYHDFLDRY LAKVSSIVS PALLVYFTI 
      APIVLCFEL FFMSKSSNF AEIAKSCVY ILGIVIELF 
      FFFCLPATY LMVQTQLMI TTVYNSGWE NCYFLSVRK 
      SLIIMMQRM QKETLLTAG KMIKINLET CTNAFRMAL 
      SFYTSLQIL DDKSRE
    CDS sequence
    • >TC007823 RA
      atggagcaa caaagagca atcatcgaa accagtcta 
      acatttttc aaagttttg ggcatacat ccactgaaa 
      accttgcgc aaaattcct gtcctgtgc ctaattagc 
      tcccatttg attttattc gccttggtg tgtttacga 
      ttcttttac gaaaaaatt actttcgga ctaatttcc 
      gacaccttc gaatctact ttcaccata gtccatgtc 
      cttgccaaa ttcatcaca cttgtgata attaaaccc 
      acgattaaa aaactgttc gaaactacc aacactttc 
      tggctgggg aaaaccttc cagaaggag tttcgacac 
      gaagttgat gcagatttg cgaactttg gggcttctt 
      ttgcgcctt tttctattt tttgtcagt attgtaatt 
      gtcacgttt gccataagg cccattttt gacgacact 
      ttggccatt cattgttac gtaccggag tttatccca 
      agagcgatt tttattgtc tttaataat gctgttttc 
      gcagtttcc gcgtttgcg gttggatcg tttgatatg 
      tttgtgtgt gttatggtc gttttgatt acagtacag 
      ttcaaaatt ctgaattat tcaattttg ggactagac 
      ttgggcaaa gttgtgaca gaaaacgac gaaaaactt 
      tgccaaaag aaaatcaaa atcatcgta gattatcat 
      gattttttg gacagatac ttggctaaa gtttcgtca 
      attgtaagt ccggccctg cttgtgtac ttcacaatt 
      gccccaatt gtcctttgc tttgaatta tttttcatg 
      tcgaaaagt tcaaacttt gccgaaatt gcaaaatcg 
      tgtgtctac atcttggga attgtgatc gaattgttt 
      ttctttttc tgtctccct gctacatac cttatggtt 
      cagacccaa ctgatgatc acaactgtt tataattcg 
      ggttgggaa aactgctat tttttatcg gttcggaaa 
      agtttgata attatgatg caacgaatg caaaaagaa 
      actcttctg acggctgga aaaatgatc aaaatcaat 
      ctagaaaca tgcactaat gcgttcaga atggcgctg 
      tctttctac acttctttg caaattttg gacgataag 
      tcacgagag tag
    mRNA sequence
    • >TC007823 RA
      tttgattta ttaattaat ttggtcgtg tataaggca 
      gtttgaacc atggagcaa caaagagca atcatcgaa 
      accagtcta acatttttc aaagttttg ggcatacat 
      ccactgaaa accttgcgc aaaattcct gtcctgtgc 
      ctaattagc tcccatttg attttattc gccttggtg 
      tgtttacga ttcttttac gaaaaaatt actttcgga 
      ctaatttcc gacaccttc gaatctact ttcaccata 
      gtccatgtc cttgccaaa ttcatcaca cttgtgata 
      attaaaccc acgattaaa aaactgttc gaaactacc 
      aacactttc tggctgggg aaaaccttc cagaaggag 
      tttcgacac gaagttgat gcagatttg cgaactttg 
      gggcttctt ttgcgcctt tttctattt tttgtcagt 
      attgtaatt gtcacgttt gccataagg cccattttt 
      gacgacact ttggccatt cattgttac gtaccggag 
      tttatccca agagcgatt tttattgtc tttaataat 
      gctgttttc gcagtttcc gcgtttgcg gttggatcg 
      tttgatatg tttgtgtgt gttatggtc gttttgatt 
      acagtacag ttcaaaatt ctgaattat tcaattttg 
      ggactagac ttgggcaaa gttgtgaca gaaaacgac 
      gaaaaactt tgccaaaag aaaatcaaa atcatcgta 
      gattatcat gattttttg gacagatac ttggctaaa 
      gtttcgtca attgtaagt ccggccctg cttgtgtac 
      ttcacaatt gccccaatt gtcctttgc tttgaatta 
      tttttcatg tcgaaaagt tcaaacttt gccgaaatt 
      gcaaaatcg tgtgtctac atcttggga attgtgatc 
      gaattgttt ttctttttc tgtctccct gctacatac 
      cttatggtt cagacccaa ctgatgatc acaactgtt 
      tataattcg ggttgggaa aactgctat tttttatcg 
      gttcggaaa agtttgata attatgatg caacgaatg 
      caaaaagaa actcttctg acggctgga aaaatgatc 
      aaaatcaat ctagaaaca tgcactaat gcgttcaga 
      atggcgctg tctttctac acttctttg caaattttg 
      gacgataag tcacgagag taggggtaa aactcctcc 
      cactc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY