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Gene information
General information
TC005489 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 12161822..12162929
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005489
    iBeetle screen iB_09308

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands partially colour darker - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection


    abdominal odoriferous glands content not present - Number of animals showing the phenotype: 5 (13 dpi)
    Days post injection



    abdominal odoriferous glands potentially not present - Number of animals showing the phenotype: 5 (13 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005489 RA
      MFFTRLIIL TLISPSWGC DYNGMIYYD LLPPVFGDY 
      LVEFSVKTY SETQMVEIL SEIPVLCTR QFNFSHPVD 
      LLLIADCGV EKIEAEFLL NTKYQPDSL SVTGNSIRT 
      IRRHTFRNL ATMFYTLRD NLIESIENE AFANLSNAK 
      FVDLAENRI TVFECNCFV NVPNLIQLI LEDNKINSL 
      KAKCFKFLQ NNGAVLVLR YNEISKIDP QVFDEMPAQ 
      NVSIHLEFN AFENLPVRI LRKHAFVVF NLTGNQIRH 
      LQFEIVENF SVGFLTVDA NLDPNLVQD LKFFAKVVL 
      YNRAQKMSP NTVFYLIFL TFCIFY
    CDS sequence
    • >TC005489 RA
      atgttcttt acacgctta ataattctc acacttatt 
      tccccaagt tggggttgc gactacaac ggaatgata 
      tactacgat cttttaccg ccagttttt ggcgattat 
      ttggtagaa tttagcgtg aaaacttat tccgaaacg 
      caaatggtc gaaatcctg agcgaaatt cctgtactg 
      tgcacccga caattcaat tttagccac cctgttgac 
      ttgctccta attgctgat tgtggagtc gaaaaaatc 
      gaagctgaa tttttatta aacacaaag tatcaaccc 
      gactcattg tcagtgact ggaaacagt atcagaaca 
      atcagaaga cacactttc agaaatctc gcaaccatg 
      ttttacact ctgcgggat aatttgatc gaaagtatc 
      gaaaatgaa gctttcgcc aatttgtcg aacgcgaag 
      ttcgtcgat ttggccgaa aatcgaatc acagttttt 
      gagtgcaac tgtttcgtc aatgttcca aatttgatt 
      cagttgatt ctagaggat aacaaaatc aacagtctc 
      aaagccaag tgtttcaaa tttttgcaa aataacggg 
      gctgttttg gtgttacgt tacaacgaa atttcgaaa 
      attgatcca caagttttt gacgaaatg cccgcccaa 
      aatgtatca attcatctc gagtttaat gcgttcgag 
      aatttaccg gttcgcatt ttgcgaaag cacgctttt 
      gtcgtgttt aacttgacc ggaaatcaa atcagacat 
      ttgcagttt gaaatcgtc gaaaatttt tcagttggt 
      tttttaacc gtcgacgca aatttggac ccaaacctt 
      gtccaagac ttgaaattt ttcgcaaaa gttgtgctg 
      tacaacagg gcacaaaaa atgtcccca aataccgtc 
      ttctacctg atttttctt acattttgt attttttat 
      taa
    mRNA sequence
    • >TC005489 RA
      atggtgcaa tcacccttt tatccactt ccaattagg 
      aacaaatac tttaatacc cactcagga tttcatgaa 
      aaattcggc actcacgaa atgttcttt acacgctta 
      ataattctc acacttatt tccccaagt tggggttgc 
      gactacaac ggaatgata tactacgat cttttaccg 
      ccagttttt ggcgattat ttggtagaa tttagcgtg 
      aaaacttat tccgaaacg caaatggtc gaaatcctg 
      agcgaaatt cctgtactg tgcacccga caattcaat 
      tttagccac cctgttgac ttgctccta attgctgat 
      tgtggagtc gaaaaaatc gaagctgaa tttttatta 
      aacacaaag tatcaaccc gactcattg tcagtgact 
      ggaaacagt atcagaaca atcagaaga cacactttc 
      agaaatctc gcaaccatg ttttacact ctgcgggat 
      aatttgatc gaaagtatc gaaaatgaa gctttcgcc 
      aatttgtcg aacgcgaag ttcgtcgat ttggccgaa 
      aatcgaatc acagttttt gagtgcaac tgtttcgtc 
      aatgttcca aatttgatt cagttgatt ctagaggat 
      aacaaaatc aacagtctc aaagccaag tgtttcaaa 
      tttttgcaa aataacggg gctgttttg gtgttacgt 
      tacaacgaa atttcgaaa attgatcca caagttttt 
      gacgaaatg cccgcccaa aatgtatca attcatctc 
      gagtttaat gcgttcgag aatttaccg gttcgcatt 
      ttgcgaaag cacgctttt gtcgtgttt aacttgacc 
      ggaaatcaa atcagacat ttgcagttt gaaatcgtc 
      gaaaatttt tcagttggt tttttaacc gtcgacgca 
      aatttggac ccaaacctt gtccaagac ttgaaattt 
      ttcgcaaaa gttgtgctg tacaacagg gcacaaaaa 
      atgtcccca aataccgtc ttctacctg atttttctt 
      acattttgt attttttat taattttga caacggtct 
      tgcagtttt ttccagaat gccaaccat tgctgtaat 
      tggtctttg gtctaaatg taaacttaa t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY