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Gene information
General information
TC005333 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 14371755..14373012
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005333
    iBeetle screen iB_09307

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005333 RA
      MSPHDSGTF ARIRKIFSI LVYTSTVVL SMAELFFNY 
      KDLETVIRA TESFFTQYG LAWKIAVFV VYKTELAQI 
      IRLCDNLWP LDEFGTGHN FQFLHKFLR RFFLLYTGN 
      LALLCTQFA VTAFFDDQF KSVMVYYGE KESRSQIYD 
      NFVFTLQVI YLYVGCFVV AGFDCFFFY LLGHAVTEL 
      KMLTISFSC KEIGRNWGY EERFKCSVK HHIHVLELL 
      DKINKVYSV MLLNQHLCS LFGICFGIF LMTKDGIPP 
      NVDHFSKWS TYIFTFILQ VWTYCFAGD QIMHWSLKI 
      PDEIFYDNY WNKYSLKNG LNKIIAIQR GQKAAGVSL 
      GGFAMLDIE SFNVVIKNA VNFFMFMDK MYKRE
    CDS sequence
    • >TC005333 RA
      atgagtccg cacgattcg ggcactttt gcccgaatc 
      cggaaaata ttttcaata ctagtttac acctcgact 
      gttgttctg tcaatggct gaactgttc tttaattac 
      aaagacctg gaaacggtg atacgggcc actgaatcg 
      ttcttcacc cagtatgga ctagcgtgg aaaattgca 
      gttttcgtg gtctacaag acagaacta gctcaaata 
      atccgactt tgtgacaat ctatggcct ttggacgaa 
      ttcggaact ggacacaat ttccagttt ctgcacaaa 
      ttcttacgc agatttttc ctactctat actggaaat 
      ttggctctt ttgtgcact caatttgca gttactgca 
      tttttcgac gatcagttc aaatctgtc atggtttat 
      tacggagaa aaggagtca cgaagccaa atttacgat 
      aatttcgtc tttactttg caagtgatt tatctctat 
      gttgggtgt tttgttgtg gcaggtttt gactgtttc 
      ttcttttat ttgttggga catgccgtt accgaattg 
      aaaatgttg acaatttcc tttagttgt aaggaaatc 
      gggaggaac tggggttat gaggagagg ttcaaatgt 
      tcagtcaag catcacatt cacgttctt gagcttttg 
      gacaaaatt aacaaagtc tattcggtc atgcttttg 
      aaccaacat ctgtgctcc ttgtttggt atttgtttt 
      gggattttt ttaatgacc aaggacggg ataccacca 
      aatgtcgat cattttagc aaatggtcg acttatatt 
      tttacgttt attcttcaa gtttggacg tattgtttc 
      gccggggac caaataatg cactggtcg ttgaaaatt 
      ccagacgaa attttttac gataattat tggaacaag 
      tattcattg aaaaacgga ttaaataaa attatagcg 
      atacaaagg gggcaaaag gcagctggg gtctcactt 
      ggaggattt gcaatgctc gatattgaa agtttcaat 
      gttgtgata aaaaatgca gtaaacttt ttcatgttt 
      atggacaaa atgtacaaa agagagtaa
    mRNA sequence
    • >TC005333 RA
      cgagttatg gaaaaagta cgtctgact gaacccctt 
      tttttgctg catattgtc gggatgagt ccgcacgat 
      tcgggcact tttgcccga atccggaaa atattttca 
      atactagtt tacacctcg actgttgtt ctgtcaatg 
      gctgaactg ttctttaat tacaaagac ctggaaacg 
      gtgatacgg gccactgaa tcgttcttc acccagtat 
      ggactagcg tggaaaatt gcagttttc gtggtctac 
      aagacagaa ctagctcaa ataatccga ctttgtgac 
      aatctatgg cctttggac gaattcgga actggacac 
      aatttccag tttctgcac aaattctta cgcagattt 
      ttcctactc tatactgga aatttggct cttttgtgc 
      actcaattt gcagttact gcatttttc gacgatcag 
      ttcaaatct gtcatggtt tattacgga gaaaaggag 
      tcacgaagc caaatttac gataatttc gtctttact 
      ttgcaagtg atttatctc tatgttggg tgttttgtt 
      gtggcaggt tttgactgt ttcttcttt tatttgttg 
      ggacatgcc gttaccgaa ttgaaaatg ttgacaatt 
      tcctttagt tgtaaggaa atcgggagg aactggggt 
      tatgaggag aggttcaaa tgttcagtc aagcatcac 
      attcacgtt cttgagctt ttggacaaa attaacaaa 
      gtctattcg gtcatgctt ttgaaccaa catctgtgc 
      tccttgttt ggtatttgt tttgggatt tttttaatg 
      accaaggac gggatacca ccaaatgtc gatcatttt 
      agcaaatgg tcgacttat atttttacg tttattctt 
      caagtttgg acgtattgt ttcgccggg gaccaaata 
      atgcactgg tcgttgaaa attccagac gaaattttt 
      tacgataat tattggaac aagtattca ttgaaaaac 
      ggattaaat aaaattata gcgatacaa agggggcaa 
      aaggcagct ggggtctca cttggagga tttgcaatg 
      ctcgatatt gaaagtttc aatgttgtg ataaaaaat 
      gcagtaaac tttttcatg tttatggac aaaatgtac 
      aaaagagag taaaaagcg ccccaatta gcaataaag 
      actcaaatt accc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY