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Gene information
General information
TC004833 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 2575179..2580076
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004833
    iBeetle screen iB_09304

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature random not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004833 RA
      MIGKYLLLW VVVTAGASV PKPRSFYNK SKSIIPDYY 
      IKEVRPPTQ GGAPVEIDF SMTVVDINS INVEDMDFR 
      MDMFLSQEW IDARIKIPE DLFEYGDDS ITLPSQYFD 
      NLWQPDLYF LNSKVVEIA TLTHKFSSV TLFRNKTIK 
      YSARMHAIV ACQMEFQHY PMDTQLCPI SIESFSYSN 
      KKMRLKWAP GGVTVSPEL KLLQYNILP LQLEEKNVY 
      TGEKSGNFS RLVVNFRFE RQIGHHLIQ TFAPSSLVV 
      VLSWFSFWL GLDAVPGRV TLLVTCLLT LVTMFTGLR 
      TDIPAVAYV KALDLWMAG CMVSVFAAL GEFVIVKVL 
      DQKYQGLKK KQCEQAMEA AITPWRPKP SIVNKRRAS 
      EGVIPMYWK NKKGKEKIL WHQVDRVSR VVFPGLFFI 
      FCAIYWTTL IFGRSATLL
    CDS sequence
    • >TC004833 RA
      atgattgga aaatattta ttattgtgg gttgtggtg 
      acagcgggg gcttcggtc cccaaacct agaagtttc 
      tataacaaa tcgaaaagc ataattccg gattattac 
      atcaaagaa gtacgacct cctactcaa ggtggtgct 
      cctgtcgaa attgatttt agtatgact gtcgtcgat 
      atcaactcc ataaatgtg gaagatatg gattttcgc 
      atggacatg tttttaagc caggaatgg atagacgca 
      agaataaaa atcccggaa gatttattc gaatatggc 
      gatgattca atcacgtta ccttcgcaa tattttgac 
      aatttgtgg caacccgat ttgtatttc ctcaactcg 
      aaagtagtc gaaattgca actctgacg cacaaattc 
      tcctcagtg actttattt cgcaacaaa acgataaaa 
      tattccgca agaatgcac gccatagtt gcgtgtcag 
      atggaattc caacactac cccatggac actcaattg 
      tgtccaata agcatcgaa agcttttcg tacagtaac 
      aaaaaaatg cgcctaaag tgggctcct ggtggcgtc 
      actgttagt ccagagtta aaactcctc caatataac 
      atcctcccg cttcaactc gaagagaaa aacgtctac 
      acgggcgaa aaaagcggg aatttttcg cgtctcgtc 
      gtcaatttc cgtttcgag cgccaaatc ggccaccat 
      ttgatccag acttttgcc ccatcgtcc ctcgtggta 
      gtactctca tggttcagt ttttggcta ggcttggat 
      gcagtacca ggacgagtt acccttcta gtcacgtgt 
      ttacttact ttggttacc atgtttaca ggcttacgg 
      accgatata ccggcagta gcatacgtc aaggcgctc 
      gatttgtgg atggcgggt tgtatggtc tcggtcttt 
      gccgcttta ggagaattt gtcattgtt aaggttttg 
      gaccagaaa tatcaaggt ttaaagaaa aagcaatgc 
      gaacaagcc atggaggct gcgattact ccctggagg 
      ccgaaaccg tctattgtt aataaaaga agggccagt 
      gaaggagtt attccgatg tattggaaa aataaaaaa 
      gggaaggaa aaaattttg tggcaccaa gtggatcga 
      gtatctcgt gtagtgttt cccggtttg tttttcatt 
      ttctgtgcg atttactgg acgactttg attttcggt 
      agaagtgcg acattgttg taa
    mRNA sequence
    • >TC004833 RA
      agaagtact ggtgtgttg gtgtaataa aatgattgg 
      aaaatattt attattgtg ggttgtggt gacagcggg 
      ggcttcggt ccccaaacc tagaagttt ctataacaa 
      atcgaaaag cataattcc ggattatta catcaaaga 
      agtacgacc tcctactca aggtggtgc tcctgtcga 
      aattgattt tagtatgac tgtcgtcga tatcaactc 
      cataaatgt ggaagatat ggattttcg catggacat 
      gtttttaag ccaggaatg gatagacgc aagaataaa 
      aatcccgga agatttatt cgaatatgg cgatgattc 
      aatcacgtt accttcgca atattttga caatttgtg 
      gcaacccga tttgtattt cctcaactc gaaagtagt 
      cgaaattgc aactctgac gcacaaatt ctcctcagt 
      gactttatt tcgcaacaa aacgataaa atattccgc 
      aagaatgca cgccatagt tgcgtgtca gatggaatt 
      ccaacacta ccccatgga cactcaatt gtgtccaat 
      aagcatcga aagcttttc gtacagtaa caaaaaaat 
      gcgcctaaa gtgggctcc tggtggcgt cactgttag 
      tccagagtt aaaactcct ccaatataa catcctccc 
      gcttcaact cgaagagaa aaacgtcta cacgggcga 
      aaaaagcgg gaatttttc gcgtctcgt cgtcaattt 
      ccgtttcga gcgccaaat cggccacca tttgatcca 
      gacttttgc cccatcgtc cctcgtggt agtactctc 
      atggttcag tttttggct aggcttgga tgcagtacc 
      aggacgagt tacccttct agtcacgtg tttacttac 
      tttggttac catgtttac aggcttacg gaccgatat 
      accggcagt agcatacgt caaggcgct cgatttgtg 
      gatggcggg ttgtatggt ctcggtctt tgccgcttt 
      aggagaatt tgtcattgt taaggtttt ggaccagaa 
      atatcaagg tttaaagaa aaagcaatg cgaacaagc 
      catggaggc tgcgattac tccctggag gccgaaacc 
      gtctattgt taataaaag aagggccag tgaaggagt 
      tattccgat gtattggaa aaataaaaa agggaagga 
      aaaaatttt gtggcacca agtggatcg agtatctcg 
      tgtagtgtt tcccggttt gtttttcat tttctgtgc 
      gatttactg gacgacttt gattttcgg tagaagtgc 
      gacattgtt gtaaatctg atgtaattt ggtcgaaaa 
      gtaggtaaa tgtaaagga aacaggttt ctagtccag 
      cgatttgtg aaatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY