Status
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Gene information
General information
TC032095
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC000683
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 7619063..7623759
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032095
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09298

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032095 RA
      MGVGFISCS FKSLVSVLS DPKGGEIVL 
      LFPGGALEC FYNQNQPQR FRCVVKQRK 
      GFVKAAFVS GAALVPVLT FGENDLLAV 
      RGSFWKHIS FITKKFPTL ASGLFYGRG 
      VFQDNFGWV PRDIPLMTV VGTPIKVSK 
      TEYPTSEQI DDLHEKFQE ELVKLFDKY 
      KYQYFDSPQ DKCLELE
    CDS sequence
    • >TC032095 RA
      atgggtgtg ggttttata tcttgttcg tttaaatcc 
      ctagttagt gttttaagt gatccgaaa ggtggcgaa 
      atcgtgctc ttgtttccc ggaggcgcg ttggaatgt 
      ttttataat cagaatcaa cctcaacgt tttcgctgt 
      gttgtcaaa caaagaaaa ggtttcgtg aaagcggct 
      ttcgtaagc ggggctgct ttagtgccg gttttaacc 
      tttggggaa aacgattta ctagctgtc agagggagc 
      ttttggaaa catatcagt tttattacg aaaaaattt 
      cctactctt gcttctgga cttttctat ggaagagga 
      gttttccaa gacaatttt ggttgggtt ccaagggat 
      ataccctta atgacagtt gttggaaca cctattaaa 
      gtttccaaa acagaatat ccaactagt gaacaaatt 
      gacgacctc cacgagaag tttcaagaa gagttggtg 
      aagttgttc gacaagtat aaataccaa tacttcgat 
      agcccccag gataaatgt cttgaattg gagtaa
    mRNA sequence
    • >TC032095 RA
      ctttagatt ctgtccatc ccacttaga agaacattt 
      aaactatat tcaaatata gttccactt caatagtaa 
      aaatgagaa tttgtggtt tgaattttg acgtttgga 
      acaccacct ttgaaaaaa aagttcaga taatactcg 
      ccggtggag catacttta cagtcgtat attcgggga 
      tcattttag taatttttt gcctttatt tattattta 
      ctcctttct ggtggttag ttctactct acttatctt 
      ggctcttcc ttgatagac atactccta gtagaggct 
      cacgcacga tcaagtttc tggacaagc ttatgtttt 
      ggaaataca tccacaacc acttcctta ccaatctac 
      gactcactc ctgactttg agctaagcc caaatcaaa 
      attacctct ttgcctgct ttccccatg gagtcgttc 
      cgatgggac ttcacgcaa ccatcgtca atccatatt 
      caaaatttc gaaacttat acccaaaat tccacgtaa 
      aagtcgcca cagtttcat tctattttc tttttccaa 
      ttttacgag atattacaa tggctgtgg ggttcatat 
      catgttcgt ttaaatctc tagttagtg ttttaagtg 
      acccaaaag gtggcgaaa ttgtggtct tacttcccg 
      gtggcgtgt tggaaagtt cttataatc agtatcaac 
      cacagcgtt accaatgcg ttataaagc acaaaaaag 
      gctttgtga aagcagctt taacaagcg gggcggctt 
      tagtcccgg tccttacgt ttggtgaaa atgatttat 
      tggccgttg aaggaagtt tctggcaga gatttaatt 
      ttattacca agcattttc cgactcttg cccttggtt 
      ttttccatg gtcgaggga ttttccaaa attattttg 
      ggatggtgc caaggaaaa ctcctctaa tgactgttg 
      gtatgtacg tatccagtg ttttattta atttgattt 
      agaaaaaat gtagttggt acaccaatc ccagtttcc 
      aaaacagaa aatccaagt gatgaacaa atcgacgct 
      ctccatacg acttttcag aaagagtta gtaaaattg 
      ttcgaaaaa tataaatac caattcttt gtacatcct 
      caaggtaaa catctagag tttgcgtaa tttttgcca 
      ataaattta aatcccatc aaggtacat agataaaaa 
      aaatagtaa acagcagtg taacattga gtgcttaat 
      tgataataa atccgtttt tgtattgac atggttatt 
      ctgtcgtca acattgtaa gacttttaa agtgagttt 
      cacgtttgc cagttaaaa tgcgaattt tcggtttaa 
      atttcgacg cctgggaca ccacatttg aaagaaaac 
      tatacatgg tattcgctg gcggagcgt attttgcaa 
      ccgtatatt cggggagcg tttcagtaa ttttttgca 
      tttgtttat tatttaccc ctttttggt ggttagttc 
      tactctaca tatcctggc tttttcttg acaggcata 
      cacctagtc aaggctccc gcactgtaa attttttgg 
      aaaacatta tattttgga aatacgtcc acaaccact 
      tccaaatta atcttaaac taactcctg attttgaac 
      taaacccaa gccaaaact acctctttg cctgctttc 
      cgcacggag ttatgccga tgggatgtc acgcaacca 
      tcgccaatt cgtattcaa aatttcgta gtttatacc 
      ccgattttc gagtaaaaa ttgctacac tttcatttt 
      tctttcttt tcccaattc tacgagatt tagcaatgg 
      gtgtgggtt ttatatctt gttcgttta aatccctag 
      ttagtgttt taagtgatc cgaaaggtg gcgaaatcg 
      tgctcttgt ttcccggag gcgcgttgg aatgttttt 
      ataatcaga atcaacctc aacgttttc gctgtgttg 
      tcaaacaaa gaaaaggtt tcgtgaaag cggctttcg 
      taagcgggg ctgctttag tgccggttt taacctttg 
      gggaaaacg atttactag ctgtcagag ggagctttt 
      ggaaacata tcagtttta ttacgaaaa aatttccta 
      ctcttgctt ctggacttt tctatggaa gaggagttt 
      tccaagaca attttggtt gggttccaa gggatatac 
      ccttaatga cagttgttg gaacaccta ttaaagttt 
      ccaaaacag aatatccaa ctagtgaac aaattgacg 
      acctccacg agaagtttc aagaagagt tggtgaagt 
      tgttcgaca agtataaat accaatact tcgatagcc 
      cccaggata aatgtcttg aattggagt aattttttt 
      gttttgatt ttgatttca acacttaaa aatccatat 
      agtattggc ttgtcaaag aatgctttt ggcaaaaat 
      ttcgtttct tgaccaaga aatatacaa ctcttgcct 
      gt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY