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Gene information
General information
TC000312 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13150637..13153664
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000312
    iBeetle screen iB_09297

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000312 RA
      MCDQYLLSY YKDEIIEIL KDQDEHKHF SVNVNFVEM 
      SGNEPNLVN DFLREPEKT LEEWNNALK RVETNLLLQ 
      FEDLYTIKN NIHCRIYSL PMYPELHRT KFPQNDDVN 
      KFLQVTGTV VRTTQMKML EYQRQYICT KCKHTMVVT 
      AEYEMKNVI TPPRQCTNP EGCKGTTIV NLGELDPTF 
      CKDYQEVKM QETVNKLDI GCMPSSIWV TLEDDLVDS 
      CKPGDNVTV CGLVKRRSS QFIIGGKID IELVIRANH 
      VHVNNNSSA VLSITPELK DMFQAFWHT YSGTPLAAR 
      DMILKSICP ELYGLFIVK LAVAVVLAG GSSQHTKSD 
      TGVRVRAEP HLLLVGDPG TGKSQLLRF ASKIIPRSV 
      FTTGVGSTA AGLTVTAVM ENGEWQLEG GALVMSDGG 
      ICCIDEFNT MKEHDRTSI HEAMEQQTI SVAKAGIVC 
      KLSTRCSIL AATNPKGNL DASQPLHMN VALASPLLS 
      RFDLILLIK DKVDDGWDS QMIDYIFTA RENSNSSKL 
      IESINWTIE TLQAYFAII KKNHPMLND DAHRILSGY 
      YQAQRRKNC RNKSRTTVR LLDSLVRLS QGHARLMFH 
      KEVEIVDAV LAVILVETA MECDSSVFN LNFDTRRDF 
      PLDPEQNYR ELAQIVLQE LQLNDLLEK EFAKGVCAL 
      CPGNANCVH KNTKMFEKI IKVCEPQPS TSKINENEK 
      INTAHNKNS DARPSSSHV NKDKIELDE KDTRKKQIG 
      ESITIANEE EIIKNKRNI TKNVNKDIT EEPVSNKKR 
      NKRENPPNP KRIKLKSKE NEIKDDLAA LNAMPSVND 
      IFRMDNEIG VTIEQNKPE ETEPSQVPS QRTKTRQFL 
      HKFRFVPKE DRPPIADET TCENLEISF KDVKSSTML 
      EPEENVKKF KFKKASKHV EMFESLEDL DDLDLDI
    CDS sequence
    • >TC000312 RA
      atgtgtgac cagtacttg ttatcgtac tacaaggac 
      gaaattatc gaaattctg aaggaccaa gatgagcac 
      aaacacttt tccgtgaat gtcaatttt gtagaaatg 
      tctggaaat gagccaaat ctggtcaac gatttttta 
      cgagagccc gagaaaacg cttgaggaa tggaataat 
      gcacttaag agagtcgaa accaactta ttgctccag 
      tttgaagat ttatacaca attaaaaat aacatacat 
      tgtcgtatc tacagccta ccgatgtat ccagagttg 
      catcgaacg aaatttccg caaaacgac gacgtgaat 
      aagttttta caagtcaca gggactgtc gttagaaca 
      acccaaatg aaaatgttg gagtatcaa aggcaatac 
      atttgcacc aagtgtaaa cacacgatg gtggtcact 
      gccgaatac gagatgaaa aatgtgata accccacca 
      cgacaatgc acaaacccg gagggttgt aaaggcaca 
      actattgtg aatttgggg gagttagac ccaacattt 
      tgtaaagac taccaagag gtcaaaatg caagaaacc 
      gtcaataaa ctcgacatt ggttgcatg ccctcctca 
      atttgggtg actctcgag gacgactta gtcgattca 
      tgcaaacct ggcgataac gtaaccgtc tgtggtttg 
      gtaaaacgc cgctcaagt caattcata atcggcggt 
      aaaatcgac atcgaacta gtaatcaga gcgaatcac 
      gtccacgtc aacaacaac agcagtgcc gtcctgtca 
      ataaccccc gaactcaag gacatgttc caagccttc 
      tggcacacc tactcgggc acgcctctc gccgcacgt 
      gacatgatc ctcaaatcg atttgtccg gagctctac 
      ggccttttc atcgtcaag ttggccgtg gcggtggtg 
      ctagctggg ggtagcagc caacacacc aaatcagac 
      acgggggtc agagtacgc gccgagccc catttatta 
      ctagtaggc gaccccgga actggcaaa tcgcaatta 
      ttaaggttt gcctcaaaa ataatcccg agatcggtt 
      ttcaccact ggggtgggg tccacggcg gcggggcta 
      acagtcact gctgttatg gaaaatggg gagtggcaa 
      ttggagggc ggggctttg gtaatgtca gacggtggg 
      atttgttgc attgatgag tttaacacg atgaaggag 
      cacgacagg acgagtatt cacgaggca atggagcaa 
      caaacgatc agtgtggcg aaggcgggg attgtgtgt 
      aagcttagt acaaggtgt tcgatattg gcggcgacc 
      aatccgaag ggaaacctt gacgctagt caacccttg 
      cacatgaac gtggctctg gcaagccct ttattgagc 
      cgttttgac ttgattttg ctcattaag gataaagtt 
      gacgacggt tgggattcc caaatgatt gattacatt 
      ttcacggca cgcgaaaat tcaaactcg tcgaaacta 
      atcgaaagt attaactgg acaatcgag actttacaa 
      gcctacttt gcgatcatt aaaaaaaat catcccatg 
      ctgaacgac gatgctcat cgaatttta agcggttat 
      taccaagcg caaaggcgc aaaaattgt cggaacaaa 
      tcaaggacc acagtaagg cttttagac agtctagtg 
      cgattatca caaggacat gcccgcttg atgtttcat 
      aaagaagtc gaaatcgtc gatgctgtc ttagctgtg 
      attttagta gaaacggca atggaatgc gattcttca 
      gtttttaat ttaaatttc gataccagg agagacttt 
      cctctcgat cccgaacaa aactaccga gaattggca 
      caaattgtg ctccaggaa ttacaactt aatgattta 
      cttgaaaaa gaatttgca aagggggtt tgtgccctc 
      tgtccaggg aacgcgaat tgtgttcac aaaaataca 
      aaaatgttt gaaaaaata attaaggtt tgtgagcca 
      cagccaagc acgtcaaag attaatgaa aatgaaaaa 
      ataaacaca gctcacaac aaaaatagt gacgcaagg 
      cctagtagt tcgcacgtt aataaagat aaaattgaa 
      ttagatgaa aaagacacc agaaaaaaa caaatagga 
      gaaagtatt acgatagca aatgaagaa gagataata 
      aaaaataaa cgaaacata acgaaaaat gtcaataaa 
      gacattacg gaagaacct gtctcaaac aagaaacga 
      aacaagagg gagaatccg ccgaacccg aagaggatt 
      aaactaaaa tcgaaggaa aacgaaata aaggacgat 
      ctagccgct ctcaatgcc atgccgagt gttaacgac 
      atttttcga atggataat gaaattggc gtaacgatt 
      gaacaaaat aaacccgaa gaaaccgaa ccaagtcaa 
      gtgccttca cagcggaca aaaactcgc caatttttg 
      cataaattt cggtttgtt cccaaagaa gatcgacct 
      cctattgcc gatgaaact acgtgtgaa aatttagaa 
      atttcgttc aaggatgtt aaatctagc acgatgtta 
      gaacctgag gagaatgtt aaaaaattc aagtttaaa 
      aaagcatca aagcatgta gaaatgttc gaatccctt 
      gaggattta gatgattta gacttggat atttaa
    mRNA sequence
    • >TC000312 RA
      atgtgtgac cagtacttg ttatcgtac tacaaggac 
      gaaattatc gaaattctg aaggaccaa gatgagcac 
      aaacacttt tccgtgaat gtcaatttt gtagaaatg 
      tctggaaat gagccaaat ctggtcaac gatttttta 
      cgagagccc gagaaaacg cttgaggaa tggaataat 
      gcacttaag agagtcgaa accaactta ttgctccag 
      tttgaagat ttatacaca attaaaaat aacatacat 
      tgtcgtatc tacagccta ccgatgtat ccagagttg 
      catcgaacg aaatttccg caaaacgac gacgtgaat 
      aagttttta caagtcaca gggactgtc gttagaaca 
      acccaaatg aaaatgttg gagtatcaa aggcaatac 
      atttgcacc aagtgtaaa cacacgatg gtggtcact 
      gccgaatac gagatgaaa aatgtgata accccacca 
      cgacaatgc acaaacccg gagggttgt aaaggcaca 
      actattgtg aatttgggg gagttagac ccaacattt 
      tgtaaagac taccaagag gtcaaaatg caagaaacc 
      gtcaataaa ctcgacatt ggttgcatg ccctcctca 
      atttgggtg actctcgag gacgactta gtcgattca 
      tgcaaacct ggcgataac gtaaccgtc tgtggtttg 
      gtaaaacgc cgctcaagt caattcata atcggcggt 
      aaaatcgac atcgaacta gtaatcaga gcgaatcac 
      gtccacgtc aacaacaac agcagtgcc gtcctgtca 
      ataaccccc gaactcaag gacatgttc caagccttc 
      tggcacacc tactcgggc acgcctctc gccgcacgt 
      gacatgatc ctcaaatcg atttgtccg gagctctac 
      ggccttttc atcgtcaag ttggccgtg gcggtggtg 
      ctagctggg ggtagcagc caacacacc aaatcagac 
      acgggggtc agagtacgc gccgagccc catttatta 
      ctagtaggc gaccccgga actggcaaa tcgcaatta 
      ttaaggttt gcctcaaaa ataatcccg agatcggtt 
      ttcaccact ggggtgggg tccacggcg gcggggcta 
      acagtcact gctgttatg gaaaatggg gagtggcaa 
      ttggagggc ggggctttg gtaatgtca gacggtggg 
      atttgttgc attgatgag tttaacacg atgaaggag 
      cacgacagg acgagtatt cacgaggca atggagcaa 
      caaacgatc agtgtggcg aaggcgggg attgtgtgt 
      aagcttagt acaaggtgt tcgatattg gcggcgacc 
      aatccgaag ggaaacctt gacgctagt caacccttg 
      cacatgaac gtggctctg gcaagccct ttattgagc 
      cgttttgac ttgattttg ctcattaag gataaagtt 
      gacgacggt tgggattcc caaatgatt gattacatt 
      ttcacggca cgcgaaaat tcaaactcg tcgaaacta 
      atcgaaagt attaactgg acaatcgag actttacaa 
      gcctacttt gcgatcatt aaaaaaaat catcccatg 
      ctgaacgac gatgctcat cgaatttta agcggttat 
      taccaagcg caaaggcgc aaaaattgt cggaacaaa 
      tcaaggacc acagtaagg cttttagac agtctagtg 
      cgattatca caaggacat gcccgcttg atgtttcat 
      aaagaagtc gaaatcgtc gatgctgtc ttagctgtg 
      attttagta gaaacggca atggaatgc gattcttca 
      gtttttaat ttaaatttc gataccagg agagacttt 
      cctctcgat cccgaacaa aactaccga gaattggca 
      caaattgtg ctccaggaa ttacaactt aatgattta 
      cttgaaaaa gaatttgca aagggggtt tgtgccctc 
      tgtccaggg aacgcgaat tgtgttcac aaaaataca 
      aaaatgttt gaaaaaata attaaggtt tgtgagcca 
      cagccaagc acgtcaaag attaatgaa aatgaaaaa 
      ataaacaca gctcacaac aaaaatagt gacgcaagg 
      cctagtagt tcgcacgtt aataaagat aaaattgaa 
      ttagatgaa aaagacacc agaaaaaaa caaatagga 
      gaaagtatt acgatagca aatgaagaa gagataata 
      aaaaataaa cgaaacata acgaaaaat gtcaataaa 
      gacattacg gaagaacct gtctcaaac aagaaacga 
      aacaagagg gagaatccg ccgaacccg aagaggatt 
      aaactaaaa tcgaaggaa aacgaaata aaggacgat 
      ctagccgct ctcaatgcc atgccgagt gttaacgac 
      atttttcga atggataat gaaattggc gtaacgatt 
      gaacaaaat aaacccgaa gaaaccgaa ccaagtcaa 
      gtgccttca cagcggaca aaaactcgc caatttttg 
      cataaattt cggtttgtt cccaaagaa gatcgacct 
      cctattgcc gatgaaact acgtgtgaa aatttagaa 
      atttcgttc aaggatgtt aaatctagc acgatgtta 
      gaacctgag gagaatgtt aaaaaattc aagtttaaa 
      aaagcatca aagcatgta gaaatgttc gaatccctt 
      gaggattta gatgattta gacttggat atttaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY