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Gene information
General information
TC000195 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 14909085..14910662
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000195
    iBeetle screen iB_09296

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000195 RA
      MFKVAKFDS NKVSEDAYK ELFKKTPPI TNRKNKNKI 
      HKGKVTKRV KEKKDVLKE EKIKSEIKA ILNNSLQSG 
      SVNLKKKKI KEKARNAPG KYVSFLSRK NNEFPQTNQ 
      TEESQQKKK KKRKRKTLS LDDNPQDLT PLDYKQKKI 
      QKVKNKLLQ AINPQPDGP PKKQKQGEK SLRERMMEK 
      LQAARFRYI NEQIYSNDS KEAQKIFKE DPDAFKAYH 
      EGYRQQVAK WPLNPLDVI IKSVKKMPK THVVADFGC 
      GDAKLAQSI KQKVHSFDL VATNEAVTA CDMAHVPLE 
      NNSVDVVVF CLSLMGTNL HDYLLEANR VLVLGGILK 
      IAEE
    CDS sequence
    • >TC000195 RA
      atgttcaaa gtggcgaag tttgatagc aataaggtt 
      tcagaagat gcctacaag gagttattt aagaagaca 
      cctcctata actaatagg aagaacaaa aataaaatt 
      cataaagga aaggtaaca aaacgtgtt aaagagaaa 
      aaagatgtt ttgaaagaa gaaaagata aagagtgag 
      ataaaagcg attttaaac aatagtctt caatcaggt 
      agtgttaat ctgaaaaag aagaaaata aaggaaaaa 
      gcaaggaac gcacctgga aaatatgtg tctttttta 
      agtcgcaaa aacaatgaa tttcctcaa accaatcaa 
      actgaagag tctcagcaa aagaaaaag aaaaaacgt 
      aaacgtaaa accctttct cttgatgac aatccgcaa 
      gacttaact ccattagat tacaagcaa aaaaagatt 
      caaaaagtg aaaaataag ttgttacaa gcaataaat 
      ccccaacct gatggccca ccaaaaaag caaaaacag 
      ggcgaaaag tcgttaagg gagcgaatg atggagaaa 
      ttacaagct gccaggttt cgatacatc aatgaacaa 
      atttactca aatgatagt aaagaagct cagaagatt 
      tttaaagag gatccagac gcgttcaag gcctatcat 
      gaaggctac agacaacag gttgccaaa tggccttta 
      aacccgctc gatgtgata ataaaaagt gtgaaaaaa 
      atgccaaaa acacacgtc gttgccgat tttggttgt 
      ggtgatgct aaattggct caatcgata aaacaaaaa 
      gtacattct tttgattta gttgccaca aatgaggcc 
      gtgactgcg tgtgatatg gcacacgtg cctttggag 
      aataatagt gttgacgtt gttgtcttt tgcttgtct 
      ctaatgggg acaaatctg cacgattat cttctggaa 
      gccaataga gtgttggtt ttgggagga attttgaaa 
      attgccgaa gagtga
    mRNA sequence
    • >TC000195 RA
      ttattaaaa taatgttaa gtaactctc agcttataa 
      aacattttt gtagagttt acgtaattt taatttgag 
      attacctgt gagttgcag catttttgc aaattttgt 
      ggcacctta accaccatc atgtttaaa tgtcaagat 
      ttgatgcta gtctttctt atttctcat gttgtgaat 
      aagaaacct aaacagtgc caagtaaca actaaaagg 
      aataaaaat gttcaaagt ggcgaagtt tgatagcaa 
      taaggtttc agaagatgc ctacaagga gttatttaa 
      gaagacacc tcctataac taataggaa gaacaaaaa 
      taaaattca taaaggaaa ggtaacaaa acgtgttaa 
      agagaaaaa agatgtttt gaaagaaga aaagataaa 
      gagtgagat aaaagcgat tttaaacaa tagtcttca 
      atcaggtag tgttaatct gaaaaagaa gaaaataaa 
      ggaaaaagc aaggaacgc acctggaaa atatgtgtc 
      ttttttaag tcgcaaaaa caatgaatt tcctcaaac 
      caatcaaac tgaagagtc tcagcaaaa gaaaaagaa 
      aaaacgtaa acgtaaaac cctttctct tgatgacaa 
      tccgcaaga cttaactcc attagatta caagcaaaa 
      aaagattca aaaagtgaa aaataagtt gttacaagc 
      aataaatcc ccaacctga tggcccacc aaaaaagca 
      aaaacaggg cgaaaagtc gttaaggga gcgaatgat 
      ggagaaatt acaagctgc caggtttcg atacatcaa 
      tgaacaaat ttactcaaa tgatagtaa agaagctca 
      gaagatttt taaagagga tccagacgc gttcaaggc 
      ctatcatga aggctacag acaacaggt tgccaaatg 
      gcctttaaa cccgctcga tgtgataat aaaaagtgt 
      gaaaaaaat gccaaaaac acacgtcgt tgccgattt 
      tggttgtgg tgatgctaa attggctca atcgataaa 
      acaaaaagt acattcttt tgatttagt tgccacaaa 
      tgaggccgt gactgcgtg tgatatggc acacgtgcc 
      tttggagaa taatagtgt tgacgttgt tgtcttttg 
      cttgtctct aatggggac aaatctgca cgattatct 
      tctggaagc caatagagt gttggtttt gggaggaat 
      tttgaaaat tgccgaaga gtgaaacga tttaatttt 
      aaaaatacc tggaaggac ttgtcccac aacttattc 
      tattttctg gactttagg aaagaaggc aacatcaaa 
      aatagaaag aaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY