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Gene information
General information
TC005817 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 7242756..7247579
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005817
    iBeetle screen iB_09294

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult nutritional state - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 2

    ovary without detectable defects - Number of animals showing the phenotype: 1

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005817 RA
      MNIIEERTY EITSKLEKE KSNLSLLTE RLKKSSQIT 
      KGIDTILNS FEERLSRLE DTILPVYND TENLQKSQV 
      NIDQTLTYL DDVISYYNV SSDVESTIE KGPGESGID 
      LDDYIHSLN RLAKAQKYF EKHIPQSVE LENVTTLFH 
      KGSDKLNSE FKTILDKYN TPMLPVVLL DLINYENSD 
      NREKLPPVQ IPDHTKAYL IKIANWLLD NGRDEYLTV 
      YGKVRGAVL QRSLTMLRN HQKSVSGGS VHGVASSPM 
      LRPKFQNRH EASRKPSTR RLHHAFERK ANRMLLKAS 
      QTLEHSTGF TLGTRRASS HLDPSYNGE EFDNEQELE 
      NYLVCVIAL HKLMQIEQA LIKGIIAPR HQPRVFELI 
      VREAMDTIV QDGENIVSR AKKCISRHD FGTVLVVFP 
      ILKQLRSLK PEFERTVEE CDLNVKSKF DAILGMLHS 
      TGAKALEDF IESLRSDSV TQLPSDGTV HELTSNVIM 
      FLEQLLDYT DTIGMVLAQ DVSYSRQLD RLKAADTNK 
      ALLGLYIKK VLVQLNHTL ISKSEQYSD PALKAIFRL 
      NNNNYVLKS LQRSSLLEL YLISEPKCE EYYYNSIQE 
      HKKAYSQSW GKLLNYIWC DDSPVNLLH GDKLRDKDR 
      AVIKEKFAG FNKEIEDIA KVQRGYSIP DVELRESIK 
      RDNKELIIP KYNSFYNMY AGVQFTKNP EKYIKHKPD 
      EVSAVIDRF FDVAA
    CDS sequence
    • >TC005817 RA
      atgaatatt atcgaggaa cgcacttat gagattaca 
      agcaaattg gagaaagag aaatctaac ctatctttg 
      ctgactgaa agactgaaa aaaagctct caaatcacg 
      aaaggcatt gatacgatt ctcaattcg tttgaggag 
      cgtctttca cggcttgaa gacactatt ttaccggtc 
      tacaatgat accgaaaat ttacaaaaa tcacaagtc 
      aatatcgac caaactctc acatatctc gacgatgtt 
      ataagctat tacaacgta tctagtgac gttgaaagc 
      acaattgag aaaggacct ggtgagagc ggtatcgat 
      ttagacgac tacatccac tcattaaat cgcctggcg 
      aaagcccag aaatatttc gaaaaacac atcccccaa 
      agcgtggaa ctcgaaaat gtcaccact ttatttcat 
      aaaggtagc gacaaactc aattccgaa tttaaaaca 
      attttggac aaatacaac actccaatg cttccggtc 
      gttctcctc gatttgatc aattacgag aattctgac 
      aaccgcgaa aagctccca ccggttcaa attccagac 
      catacgaaa gcctatttg attaaaatc gcaaattgg 
      ctacttgac aacggccga gatgaatat ttaacagtt 
      tatggtaaa gtgcgtggg gctgtttta cagcggtct 
      ttgaccatg ttacgcaac caccagaag agcgtaagc 
      ggtgggagt gtccatggt gttgccagt tcaccaatg 
      ctgcgcccc aaatttcaa aaccgacat gaagctagt 
      cgcaagcca tcaacgcgc cgcctccac cacgccttc 
      gaacgcaaa gccaatcgc atgctttta aaagcatcg 
      caaacgctt gaacattcg acaggtttc actctcggc 
      acccgacgc gcctcctcc cacctcgac ccctcctac 
      aacggggag gagttcgac aacgagcaa gaactggag 
      aattatcta gtgtgcgtg atcgcacta cataaactg 
      atgcaaatc gagcaggcc ttgatcaag ggcataatc 
      gcgccaagg caccagccc cgagttttc gaattgata 
      gtcagggag gccatggac acaatcgtc caagatggc 
      gagaatata gtctcgcgt gcgaagaaa tgcatcagt 
      cggcacgat ttcggcaca gttttggtc gttttcccg 
      attttgaag cagctgcgt tcgctgaaa cccgagttt 
      gaacgaacg gtggaggag tgcgatttg aatgtcaag 
      tcgaagttt gacgccatt ttggggatg ttgcactcg 
      acgggggcg aaagcactc gaggacttc atcgagagt 
      ttacggtcc gattcggtg acgcagttg cctagcgac 
      gggaccgtg cacgagctc acaagcaac gtgatcatg 
      tttttggaa caattgctc gattatacc gacactatt 
      ggcatggtt ctggcacag gatgttagt tattcgaga 
      caattggac cgtttgaag gccgctgac acgaacaag 
      gcccttctg ggcctttat attaagaag gttttagtg 
      cagttgaac cacacgttg attagtaaa agcgaacag 
      tatagtgac ccagctttg aaggcaatt ttccgctta 
      aataacaac aattatgtg ctgaagtcg ctccagcgc 
      agctcatta ctggagttg tacctaatt tccgaaccg 
      aaatgtgag gaatattac tataactct atccaagaa 
      cacaagaaa gcttactcg caaagttgg ggcaaactc 
      ttgaactac atctggtgt gacgactcg cctgttaat 
      cttttgcac ggtgataag ctccgtgat aaagacagg 
      gccgtaatt aaggagaaa tttgctggt tttaataaa 
      gaaattgaa gacatcgcc aaagttcag cgcggatat 
      tcgattcca gacgtcgaa ttgcgcgaa agtatcaaa 
      agggataat aaagagttg attattccg aaatacaat 
      tcgttttat aacatgtat gcaggtgtc caatttaca 
      aagaacccg gaaaagtac atcaagcat aagcctgat 
      gaggtgtcg gcggttatt gaccgtttc tttgatgtg 
      gcggcctaa
    mRNA sequence
    • >TC005817 RA
      ggatttgcc aattgtcaa gtttgttga tttagtaac 
      aaaagacgt caataatgt tttgctcgt aaagtcttc 
      aattttgga aataattct ccaaaatat tgggattaa 
      acaagatga atattatcg aggaacgca cttatgaga 
      ttacaagca aattggaga aagagaaat ctaacctat 
      ctttgctga ctgaaagac tgaaaaaaa gctctcaaa 
      tcacgaaag gcattgata cgattctca attcgtttg 
      aggagcgtc tttcacggc ttgaagaca ctattttac 
      cggtctaca atgataccg aaaatttac aaaaatcac 
      aagtcaata tcgaccaaa ctctcacat atctcgacg 
      atgttataa gctattaca acgtatcta gtgacgttg 
      aaagcacaa ttgagaaag gacctggtg agagcggta 
      tcgatttag acgactaca tccactcat taaatcgcc 
      tggcgaaag cccagaaat atttcgaaa aacacatcc 
      cccaaagcg tggaactcg aaaatgtca ccactttat 
      ttcataaag gtagcgaca aactcaatt ccgaattta 
      aaacaattt tggacaaat acaacactc caatgcttc 
      cggtcgttc tcctcgatt tgatcaatt acgagaatt 
      ctgacaacc gcgaaaagc tcccaccgg ttcaaattc 
      cagaccata cgaaagcct atttgatta aaatcgcaa 
      attggctac ttgacaacg gccgagatg aatatttaa 
      cagtttatg gtaaagtgc gtggggctg ttttacagc 
      ggtctttga ccatgttac gcaaccacc agaagagcg 
      taagcggtg ggagtgtcc atggtgttg ccagttcac 
      caatgctgc gccccaaat ttcaaaacc gacatgaag 
      ctagtcgca agccatcaa cgcgccgcc tccaccacg 
      ccttcgaac gcaaagcca atcgcatgc ttttaaaag 
      catcgcaaa cgcttgaac attcgacag gtttcactc 
      tcggcaccc gacgcgcct cctcccacc tcgacccct 
      cctacaacg gggaggagt tcgacaacg agcaagaac 
      tggagaatt atctagtgt gcgtgatcg cactacata 
      aactgatgc aaatcgagc aggccttga tcaagggca 
      taatcgcgc caaggcacc agccccgag ttttcgaat 
      tgatagtca gggaggcca tggacacaa tcgtccaag 
      atggcgaga atatagtct cgcgtgcga agaaatgca 
      tcagtcggc acgatttcg gcacagttt tggtcgttt 
      tcccgattt tgaagcagc tgcgttcgc tgaaacccg 
      agtttgaac gaacggtgg aggagtgcg atttgaatg 
      tcaagtcga agtttgacg ccattttgg ggatgttgc 
      actcgacgg gggcgaaag cactcgagg acttcatcg 
      agagtttac ggtccgatt cggtgacgc agttgccta 
      gcgacggga ccgtgcacg agctcacaa gcaacgtga 
      tcatgtttt tggaacaat tgctcgatt ataccgaca 
      ctattggca tggttctgg cacaggatg ttagttatt 
      cgagacaat tggaccgtt tgaaggccg ctgacacga 
      acaaggccc ttctgggcc tttatatta agaaggttt 
      tagtgcagt tgaaccaca cgttgatta gtaaaagcg 
      aacagtata gtgacccag ctttgaagg caattttcc 
      gcttaaata acaacaatt atgtgctga agtcgctcc 
      agcgcagct cattactgg agttgtacc taatttccg 
      aaccgaaat gtgaggaat attactata actctatcc 
      aagaacaca agaaagctt actcgcaaa gttggggca 
      aactcttga actacatct ggtgtgacg actcgcctg 
      ttaatcttt tgcacggtg ataagctcc gtgataaag 
      acagggccg taattaagg agaaatttg ctggtttta 
      ataaagaaa ttgaagaca tcgccaaag ttcagcgcg 
      gatattcga ttccagacg tcgaattgc gcgaaagta 
      tcaaaaggg ataataaag agttgatta ttccgaaat 
      acaattcgt tttataaca tgtatgcag gtgtccaat 
      ttacaaaga acccggaaa agtacatca agcataagc 
      ctgatgagg tgtcggcgg ttattgacc gtttctttg 
      atgtggcgg cctaaatat agaatttga aagtttgat 
      aaataaata aatttttcc gaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY