Status
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Gene information
General information
TC005854 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6412418..6432364
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC005854
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09292

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC005854 RA
      MSALEVLVR VFFVSALVG FCCPEEAPM TRAERHRLR 
      EETRDMFYH AYWAYMENA YPADELMPL SCKGRFRGL 
      TPNRGDIDD CLGNFSLTL IDTLDSLVV LGDLEEFEH 
      AVKLVIKDV SFDNDVVVS VFETNIRVL GGLLSAHIL 
      ADYLQQRDG IMPWYKGEL LNMAKDVGY RLLPAFNTT 
      TGIPHSRVN MKYGLKSDR LESARETCT ACAGSMILE 
      MAALSRLTG EPIFEEKAH KAMDELWKM RHRSSDLMG 
      TVLNVHSGD WVRRDSGVG AGIDSYYEY CLKAYILLG 
      DNKYLHRFN RHYNAVMKY ISQGPMLLD VHMHRPHTN 
      SRNYMDALL AFWPGLQVL KGDLKPAVE THEMLYQVM 
      QRHTFIPEA FTTDFQVHW GNHPLRPEF LESTYFLYG 
      ATNDPYYLE VGRTVLNSL QKYARVPCG YAAVNDVRT 
      RKQEDRMDS FVLSETLKY LYLLFADKE DLILNLDEF 
      IFTTEGHLL PLSLAGYNG NKTVSELDV EEGEFARIC 
      PNTLQLFPE TVRKPLQNM VDGMCPKRN TKRRLTAVE 
      FSASNINHL KMIKDMGIN IIALSDGRV QLLHTFSSA 
      RSSADAEEG LLFMQEMME LSKLQTQQL DATPQSVTF 
      PIKDQTLTV QACPSQFGK NLQGDQKIT ARTIIIHPF 
      RACSDLVQT EAIKGKIAI MERGDCMFV DKARKVQKH 
      GAVAAIIID NTPGSSAAS SPMFSMSGD GTDDVAIPT 
      VFLFAQDAS KLLFALSRD PSIEVTIGE YKNEGEAWA 
      HSEEESMFH KLKVSVQEF LNKHTGIAF SKSVKVGAF 
      RASLGSDKI RITYEEGEE GGKVAGVGD TVTTPQWVQ 
      VRRDLLKSI MTSEGKELY VPLNILRIY YQTLSDDPM 
      EDKRVQDTV KQAEWLLNQ LSIELANKD ALVKPEDGV 
      KVLSVLTDL GQLAGSDLE DKETLKALN SILESVAKD 
      VKFKKEHLG AVMKKLQEA DDDLVVSDV KPKETDRDS 
      SEINSSDSE HIQTKISHA VDEL
    CDS sequence
    • >TC005854 RA
      atgagtgct ttggaggtg ctagtgcgg gtgtttttc 
      gtgagtgct ttggtgggc ttctgctgc ccggaggag 
      gcccccatg accagagcg gagcgccac cggctgagg 
      gaggagacc cgggacatg ttctaccac gcgtactgg 
      gcctacatg gagaacgcg tatcctgcc gacgagctg 
      atgcccctg agctgcaag ggcaggttc agggggctc 
      acccccaac cggggggac atcgacgac tgcctgggc 
      aatttctca cttacacta attgatact ctggactct 
      ttagtggtg ttaggtgat ttagaagaa ttcgaacat 
      gccgtcaag cttgtaata aaagacgtt tcgtttgat 
      aacgatgtc gtcgtttca gttttcgaa acaaatatt 
      cgagtttta ggtggactt ttatcagcc cacattttg 
      gccgattat ttgcaacaa agggacggg attatgccc 
      tggtataag ggcgaactt ctcaacatg gccaaagat 
      gtgggctat cggctttta ccggcattt aacaccacg 
      acaggaata ccacattcg agggtgaat atgaaatat 
      gggttaaag agcgataga ttggagtcg gcgagggag 
      acgtgtaca gcatgtgct gggtctatg attttagag 
      atggcagca ctgtctcga ctgactgga gaaccgatt 
      ttcgaggag aaggctcat aaagcgatg gatgagttg 
      tggaagatg agacatcga agttcggat ttaatggga 
      actgttttg aatgttcat tcgggagat tgggttcga 
      agggattcg ggagtggga gctgggatt gattcctat 
      tacgagtac tgtctcaag gcgtatatt ttgctgggg 
      gataacaag tacttgcat aggtttaat cggcactat 
      aatgcagtc atgaagtat atatcgcaa ggtccgatg 
      ttgcttgac gtgcacatg caccgcccc cacaccaac 
      tcccgcaac tacatggac gccctcctc gccttctgg 
      cccggcctc caggtgctc aaaggcgac ctcaaacca 
      gccgtcgaa acccacgag atgctctac caggtcatg 
      cagcgccac accttcatc cccgaagcc ttcaccacc 
      gacttccag gtccactgg ggcaaccac cccctccga 
      cccgaattt ctcgaatcc acttacttc ctctacggt 
      gccacaaac gacccctac tatctcgaa gtgggcagg 
      accgtactc aacagttta caaaagtac gcccgagtg 
      ccttgcggc tatgccgcc gttaacgac gtccggaca 
      cgcaaacag gaggatcgg atggattcg tttgtgttg 
      tccgaaacg ttgaaatac ttgtatttg ttgtttgcc 
      gataaggag gatttgatt ttgaatttg gatgagttt 
      atttttacg acggaaggt catttgttg ccgttgagt 
      ttggcgggg tacaatggt aataagact gtgagcgaa 
      ttggatgtg gaggagggg gagtttgcg aggatttgt 
      cccaatacg ttacagttg ttccccgaa acggtgagg 
      aagccgtta cagaatatg gtcgatggg atgtgtccc 
      aagaggaat acgaagagg aggctgaca gctgtcgag 
      ttctcggcc tcgaatatc aatcatttg aagatgatt 
      aaggatatg gggatcaac attattgcg ttgagtgat 
      ggtcgggtg cagctgcta cacacgttt tcatcggcc 
      cgttcgagc gccgacgcc gaagagggc ctcctcttc 
      atgcaagaa atgatggaa ctctcgaaa ctccaaacc 
      caacaactg gacgccacc ccccaatca gtcaccttt 
      cccatcaaa gaccaaacc ctcacagtc caggcctgc 
      ccctcgcag ttcggcaag aacctccaa ggcgaccag 
      aaaatcacc gctcgcacc atcatcatc cacccgttc 
      cgcgcctgc tccgacctc gtccagacc gaggccatc 
      aaggggaaa atcgccatc atggagcgc ggcgactgc 
      atgttcgtc gacaaggcg cgcaaagtg caaaagcac 
      ggtgccgtg gccgccatc atcatcgac aacacacca 
      ggaagcagc gccgcctcc tcgccgatg ttctccatg 
      tcgggcgac ggcaccgac gacgtcgcc atccccacg 
      gtgttcctg ttcgcccag gacgcctcc aagctgctg 
      ttcgcgctg agcagggac cccagcatc gaagtcacc 
      ataggcgag tataaaaac gaaggtgag gcgtgggcg 
      cacagcgag gaggaaagt atgtttcat aaattgaaa 
      gtctccgtc caggagttt ttgaataaa cacaccggg 
      attgcgttc agtaagtcg gtcaaagtt ggcgcgttt 
      agggcgagt ttagggtct gataaaata cggataacg 
      tacgaggag ggcgaggag ggggggaag gtcgccggg 
      gtgggagat acagtgacc acgccccag tgggtgcaa 
      gtccgtagg gatttgctc aagtcgatt atgacgtca 
      gaggggaag gaattgtac gtccctttg aatattttg 
      cggatttat tatcaaaca ttaagtgat gatccgatg 
      gaagataag agagtgcaa gatacggtc aagcaggcc 
      gagtggttg ctcaatcaa ctgtccatt gaattggcc 
      aataaggac gcgttggtt aaaccggag gatggggtt 
      aaggtgttg tcggtatta accgacttg ggtcagttg 
      gctgggagc gatctcgag gataaggaa acactaaag 
      gcgctgaat tcgattctg gaaagtgtg gccaaggat 
      gttaagttc aagaaggaa catttgggt gcggttatg 
      aagaagttg caagaggcg gatgatgat ttggtggtc 
      agtgatgtt aagcccaaa gagacggac agggattct 
      agtgaaatt aatagtagt gatagtgaa catattcag 
      acgaaaatc agtcacgcc gttgatgag ttatga
    mRNA sequence
    • >TC005854 RA
      cgtatgatc ataaaaaaa gttcagttg agcataaaa 
      atcatatta gatttttgt ttcaggcaa cttttttag 
      tttttttgt aaatttttt attgttttt aaaaaaaaa 
      cagtatgta aggttggta tgtcagtga acgtctcct 
      ccactattt gatgttttt atttcaatt tttaaatga 
      attttgcac tttttatcg ctttaattt aattaccgt 
      tgttcctat aaacccatt cgaggccac cttgtatcg 
      ttttgacag ctccatttc gtccaatca cagcgcgtg 
      tcgtagcgc cgcccctct cgcagccag ctgagatcg 
      gaacagtca tcccagttc tttcacaaa agttgacac 
      atttccacc aaaatgtct caaaattcc cacaatcat 
      gccaaattc cccgtgtct gtgtgatcc ctcatcgtc 
      agtgcagcc cctttgacg tgtggaacg atgcccgca 
      gcccccgag acgggaccc tcctcaggc aattgttcc 
      cagcgataa gaacctctt atcacctgt cagataaat 
      tttcacgaa acaatatgt tactgagcg ctgaggcgt 
      actcctgat aaaccgcaa aatgagtgc tttggaggt 
      gctagtgcg ggtgttttt cgtgagtgc tttggtggg 
      cttctgctg cccggagga ggcccccat gaccagagc 
      ggagcgcca ccggctgag ggaggagac ccgggacat 
      gttctacca cgcgtactg ggcctacat ggagaacgc 
      gtatcctgc cgacgagct gatgcccct gagctgcaa 
      gggcaggtt cagggggct cacccccaa ccgggggga 
      catcgacga ctgcctggg caatttctc acttacact 
      aattgatac tctggactc tttagtggt gttaggtga 
      tttagaaga attcgaaca tgccgtcaa gcttgtaat 
      aaaagacgt ttcgtttga taacgatgt cgtcgtttc 
      agttttcga aacaaatat tcgagtttt aggtggact 
      tttatcagc ccacatttt ggccgatta tttgcaaca 
      aagggacgg gattatgcc ctggtataa gggcgaact 
      tctcaacat ggccaaaga tgtgggcta tcggctttt 
      accggcatt taacaccac gacaggaat accacattc 
      gagggtgaa tatgaaata tgggttaaa gagcgatag 
      attggagtc ggcgaggga gacgtgtac agcatgtgc 
      tgggtctat gattttaga gatggcagc actgtctcg 
      actgactgg agaaccgat tttcgagga gaaggctca 
      taaagcgat ggatgagtt gtggaagat gagacatcg 
      aagttcgga tttaatggg aactgtttt gaatgttca 
      ttcgggaga ttgggttcg aagggattc gggagtggg 
      agctgggat tgattccta ttacgagta ctgtctcaa 
      ggcgtatat tttgctggg ggataacaa gtacttgca 
      taggtttaa tcggcacta taatgcagt catgaagta 
      tatatcgca aggtccgat gttgcttga cgtgcacat 
      gcaccgccc ccacaccaa ctcccgcaa ctacatgga 
      cgccctcct cgccttctg gcccggcct ccaggtgct 
      caaaggcga cctcaaacc agccgtcga aacccacga 
      gatgctcta ccaggtcat gcagcgcca caccttcat 
      ccccgaagc cttcaccac cgacttcca ggtccactg 
      gggcaacca ccccctccg acccgaatt tctcgaatc 
      cacttactt cctctacgg tgccacaaa cgaccccta 
      ctatctcga agtgggcag gaccgtact caacagttt 
      acaaaagta cgcccgagt gccttgcgg ctatgccgc 
      cgttaacga cgtccggac acgcaaaca ggaggatcg 
      gatggattc gtttgtgtt gtccgaaac gttgaaata 
      cttgtattt gttgtttgc cgataagga ggatttgat 
      tttgaattt ggatgagtt tatttttac gacggaagg 
      tcatttgtt gccgttgag tttggcggg gtacaatgg 
      taataagac tgtgagcga attggatgt ggaggaggg 
      ggagtttgc gaggatttg tcccaatac gttacagtt 
      gttccccga aacggtgag gaagccgtt acagaatat 
      ggtcgatgg gatgtgtcc caagaggaa tacgaagag 
      gaggctgac agctgtcga gttctcggc ctcgaatat 
      caatcattt gaagatgat taaggatat ggggatcaa 
      cattattgc gttgagtga tggtcgggt gcagctgct 
      acacacgtt ttcatcggc ccgttcgag cgccgacgc 
      cgaagaggg cctcctctt catgcaaga aatgatgga 
      actctcgaa actccaaac ccaacaact ggacgccac 
      cccccaatc agtcacctt tcccatcaa agaccaaac 
      cctcacagt ccaggcctg cccctcgca gttcggcaa 
      gaacctcca aggcgacca gaaaatcac cgctcgcac 
      catcatcat ccacccgtt ccgcgcctg ctccgacct 
      cgtccagac cgaggccat caaggggaa aatcgccat 
      catggagcg cggcgactg catgttcgt cgacaaggc 
      gcgcaaagt gcaaaagca cggtgccgt ggccgccat 
      catcatcga caacacacc aggaagcag cgccgcctc 
      ctcgccgat gttctccat gtcgggcga cggcaccga 
      cgacgtcgc catccccac ggtgttcct gttcgccca 
      ggacgcctc caagctgct gttcgcgct gagcaggga 
      ccccagcat cgaagtcac cataggcga gtataaaaa 
      cgaaggtga ggcgtgggc gcacagcga ggaggaaag 
      tatgtttca taaattgaa agtctccgt ccaggagtt 
      tttgaataa acacaccgg gattgcgtt cagtaagtc 
      ggtcaaagt tggcgcgtt tagggcgag tttagggtc 
      tgataaaat acggataac gtacgagga gggcgagga 
      gggggggaa ggtcgccgg ggtgggaga tacagtgac 
      cacgcccca gtgggtgca agtccgtag ggatttgct 
      caagtcgat tatgacgtc agaggggaa ggaattgta 
      cgtcccttt gaatatttt gcggattta ttatcaaac 
      attaagtga tgatccgat ggaagataa gagagtgca 
      agatacggt caagcaggc cgagtggtt gctcaatca 
      actgtccat tgaattggc caataagga cgcgttggt 
      taaaccgga ggatggggt taaggtgtt gtcggtatt 
      aaccgactt gggtcagtt ggctgggag cgatctcga 
      ggataagga aacactaaa ggcgctgaa ttcgattct 
      ggaaagtgt ggccaagga tgttaagtt caagaagga 
      acatttggg tgcggttat gaagaagtt gcaagaggc 
      ggatgatga tttggtggt cagtgatgt taagcccaa 
      agagacgga cagggattc tagtgaaat taatagtag 
      tgatagtga acatattca gacgaaaat cagtcacgc 
      cgttgatga gttatgatg gagatattt ataattttt 
      gttgatttt tttttgatt ggtctcaag aggagcatt 
      tctgtccta taccctact ttatacaac ttgtacatt 
      gcatcatgt aaatatact ctatttata aagctgaat 
      gtataaaaa ttgtgagaa tatttttat attattata 
      taaaatgtt gaacataaa ccaataaat tttattaca 
      aacg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY