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Gene information
General information
TC034022
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005866
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6190693..6193850
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034022
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09291

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larva segment random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034022 RA
      MFARNSVFG NNFEILREN LRDPARPNF DSEKCLNDL 
      RPVEEIPQP YQEVFAEYL CFNPIQSKL IDDAFYTDQ 
      SIVVSAPTG SGKTAIFEL AIVRLLIAS ESSQKFKIV 
      YICPMKALC EERLVDWNK KFSNFGINP ISVTGDSEN 
      IDFQSLRNY NLIITTPEK WDCLTRKWR ENLDLVEIV 
      KLFMIDEVH LLNEECRGS TLETIVCRM KTIEESVKT 
      HDLNHKIRF IAVSATIAN IEDIAEWNN AKSFKFSDD 
      FRPVRLNKI VLGYSEPPK STPFKFDLA LNYKLHSLM 
      MQYSHGKPT LIFCSTRKI VEMTARHIV QHLTIGLKP 
      EQKQRIVEV ASTISDAKA KETLIHGVG YHHAGMLPE 
      TRRAIENLF RNNELPVLV TTSTLAMGV NLPAHLGLY 
      RNGPFANDR KGRSAPV
    CDS sequence
    • >TC034022 RA
      atgtttgcc cgaaattcg gtttttgga aataatttt 
      gagattttg agagaaaat cttagagac cctgctagg 
      ccaaacttt gacagcgag aagtgtctt aatgatttg 
      cgacctgtt gaagaaatc cctcagcct taccaagaa 
      gttttcgca gaatatctc tgcttcaat ccgattcag 
      tcaaaacta atcgacgat gctttttac acagaccaa 
      tcaatagtc gtgtctgca ccgactggt tcaggaaaa 
      acggcaatt ttcgaacta gcaatagtc cgacttttg 
      atagcttcc gagtcatca caaaaattt aaaattgtc 
      tacatttgt cccatgaaa gccctatgt gaggagcga 
      ttagtagat tggaacaaa aaattctca aattttggc 
      ataaaccca atttcagtg accggcgat agcgaaaat 
      atcgacttc caaagtctc cgaaactac aaccttata 
      atcacaacc ccggaaaag tgggactgt ctcacacgc 
      aaatggcgc gagaacctc gacttggtt gaaatcgta 
      aaactcttc atgattgac gaagttcat ctgctcaat 
      gaagagtgt cgtggatcg actctcgaa acaattgtt 
      tgccggatg aaaaccatc gaagaatcg gttaaaacg 
      cacgattta aaccataaa atccgcttc attgctgtt 
      tctgcaacc atcgcaaac atcgaggat atcgccgag 
      tggaacaat gccaagtct ttcaaattc tccgacgat 
      tttcgccca gttaggctc aacaaaatc gtcttaggg 
      tactcagaa cccccgaaa tcgactccc ttcaagttc 
      gatttggcc ctaaactac aaattgcac tccttgatg 
      atgcaatat tcgcacggg aagcccact cttattttt 
      tgcagtacc cggaagatc gtggaaatg acggccaga 
      cacatagtc caacacttg acaatcggt ctaaaaccg 
      gagcaaaag cagcgcatt gtagaagta gcatcgaca 
      atttccgac gctaaagcc aaagaaacc ctcattcat 
      ggtgtgggg taccaccat gctggtatg ctgccggaa 
      acgagacgt gcgatcgaa aacttattt cgcaacaat 
      gaacttccg gttttggtc acgactagc acactagca 
      atgggtgtg aacctccca gcccatttg ggattatac 
      cgaaacggc ccttttgca aatgatcgg aagggccgg 
      tcggcccca gtatga
    mRNA sequence
    • >TC034022 RA
      actgtgact tgctggggc aaaaatgtt tgcccgaaa 
      ttcggtttt tggaaataa ttttgagat tttgagaga 
      aaatcttag agaccctgc taggccaaa ctttgacag 
      cgagaagtg tcttaatga tttgcgacc tgttgaaga 
      aatccctca gccttacca agaagtttt cgcagaata 
      tctctgctt caatccgat tcagtcaaa actaatcga 
      cgatgcttt ttacacaga ccaatcaat agtcgtgtc 
      tgcaccgac tggttcagg aaaaacggc aattttcga 
      actagcaat agtccgact tttgatagc ttccgagtc 
      atcacaaaa atttaaaat tgtctacat ttgtcccat 
      gaaagccct atgtgagga gcgattagt agattggaa 
      caaaaaatt ctcaaattt tggcataaa cccaatttc 
      agtgaccgg cgatagcga aaatatcga cttccaaag 
      tctccgaaa ctacaacct tataatcac aaccccgga 
      aaagtggga ctgtctcac acgcaaatg gcgcgagaa 
      cctcgactt ggttgaaat cgtaaaact cttcatgat 
      tgacgaagt tcatctgct caatgaaga gtgtcgtgg 
      atcgactct cgaaacaat tgtttgccg gatgaaaac 
      catcgaaga atcggttaa aacgcacga tttaaacca 
      taaaatccg cttcattgc tgtttctgc aaccatcgc 
      aaacatcga ggatatcgc cgagtggaa caatgccaa 
      gtctttcaa attctccga cgattttcg cccagttag 
      gctcaacaa aatcgtctt agggtactc agaaccccc 
      gaaatcgac tcccttcaa gttcgattt ggccctaaa 
      ctacaaatt gcactcctt gatgatgca atattcgca 
      cgggaagcc cactcttat tttttgcag tacccggaa 
      gatcgtgga aatgacggc cagacacat agtccaaca 
      cttgacaat cggtctaaa accggagca aaagcagcg 
      cattgtaga agtagcatc gacaatttc cgacgctaa 
      agccaaaga aaccctcat tcatggtgt ggggtacca 
      ccatgctgg tatgctgcc ggaaacgag acgtgcgat 
      cgaaaactt atttcgcaa caatgaact tccggtttt 
      ggtcacgac tagcacact agcaatggg tgtgaacct 
      cccagccca tttgggatt ataccgaaa cggcccttt 
      tgcaaatga tcggaaggg ccggtcggc cccagtatg 
      acactgaag ccactgccc ttatcctaa ccacatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY