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Gene information
General information
TC006274 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 6071585..6107092
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006274
    iBeetle screen iB_09290

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    eggs lost during procedure: potentially due to defects in egg-shells egg-morphology or egg-size, (13 dpi)
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006274 RA
      MRVSNFFLI LLVNLGEIC VAILNSGPS YRNGGDILP 
      RFASQPNTF SAVIGDTIV LPCEVQNLG SFVIVWKRG 
      ITLLTAGQQ KITADPRIS LVGYNLEVR DIRYSDQGD 
      YTCQIGDGS HGDLIHTVE ILMPPSIQI FPNNGDVLT 
      TRKGATVSF ECRASGNPT PVVQWSKKE GLLPSGLQV 
      QTGYLLSLT NVQREDAGA YQCTASNGI GQAVTGEIK 
      LHVLFPPEI TVAKSWVNS GEGLEARLD CVVQADPPG 
      EVSWYQNSF PLQQTDRRI MSSKGKIYT LTIKNVQFS 
      DFGNYSCTV HNSIGKDRK YIELTGKPG PARIISPAY 
      SNPDYYDLR WVVQSVLPI IEVKILYRR INATTFYDH 
      KGQWHDLVI KPEQKYDAK TSERLQSFR ITNLVPDSL 
      YECLILTKN QHGYSDVSD VHQWFSSDK GRPFVGSTG 
      QIHCLGIAV MILLQIPNV LL
    CDS sequence
    • >TC006274 RA
      atgcgcgtt tccaatttt ttcctcatc ctgctagtg 
      aatctgggg gaaatttgc gtggctatc ctcaattcc 
      ggcccgagt taccggaac gggggcgac attctgccc 
      agattcgcc tctcagccc aacaccttc agcgccgtg 
      atcggcgac accatcgtg ctgccatgc gaggtgcag 
      aatctgggt tcgttcgtt atagtgtgg aaacgaggc 
      atcactctg ctaacggcc ggacaacaa aaaatcaca 
      gccgatccc agaatatca ctagtggga tataatctg 
      gaagtgagg gatatcagg tactcggat cagggagac 
      tacacgtgc cagattggc gacggctcg cacggggac 
      ctcattcac actgttgag atactcatg cctccgagt 
      atacaaatt tttccgaac aatggggac gttctgacc 
      acccggaag ggggcgacg gtgtcgttc gagtgccgc 
      gccagcggg aacccaaca ccggtggtc cagtggtcg 
      aaaaaagaa ggtctgcta ccgtctggg ttacaagtc 
      caaacaggg tatctctta tcactaacc aacgtccaa 
      cgcgaagat gcgggcgct tatcagtgc acggcttcc 
      aacggtata gggcaggcg gtcactgga gaaatcaag 
      ctccacgtc ctttttccg ccggaaatt accgtcgcc 
      aagtcgtgg gtgaactcc ggggaaggc ctggaggcc 
      cgattggac tgcgtcgtc caagcggac ccccccgga 
      gaggtttct tggtaccaa aattccttc cctttgcaa 
      caaaccgat cggagaatc atgtcaagt aaaggcaaa 
      atctacact ctgacaata aaaaatgtc cagttctcc 
      gatttcggg aattacagt tgtacggtg cacaattcg 
      atcggaaaa gaccggaaa tatatcgaa ttgaccgga 
      aagcccgga ccggcacgt atcataagt ccggcgtat 
      tcgaacccg gattattac gacttgaga tgggtagtg 
      cagtcggtg ctgccaatc attgaagtg aaaattctc 
      taccgacga atcaatgca acgactttt tacgaccat 
      aaaggccag tggcacgat ctagttata aagccggag 
      caaaagtac gatgcgaaa accagtgag aggttgcag 
      agctttcgc ataacgaat ttagtgcca gattcgttg 
      tacgaatgt ctgattttg accaaaaac cagcatggg 
      tacagtgac gtctcggat gtccatcag tggttcagt 
      tcggacaaa ggacgaccg ttcgttggg tcaacagga 
      caaattcat tgcttggga attgcagta atgatattg 
      ttgcaaatc cccaacgtt ttattgtag
    mRNA sequence
    • >TC006274 RA
      tcagtagta ttcaagcag ccgtcgagt ttcagggag 
      cgattcatg ccgttttca ggcgcgtgt ttcgtgtaa 
      tttaaaagc tcgcagcat gtcatcatc ctcccgcga 
      aatgccgaa aatcgagtg agccaagat gcgcgtttc 
      caatttttt cctcatcct gctagtgaa tctggggga 
      aatttgcgt ggctatcct caattccgg cccgagtta 
      ccggaacgg gggcgacat tctgcccag attcgcctc 
      tcagcccaa caccttcag cgccgtgat cggcgacac 
      catcgtgct gccatgcga ggtgcagaa tctgggttc 
      gttcgttat agtgtggaa acgaggcat cactctgct 
      aacggccgg acaacaaaa aatcacagc cgatcccag 
      aatatcact agtgggata taatctgga agtgaggga 
      tatcaggta ctcggatca gggagacta cacgtgcca 
      gattggcga cggctcgca cggggacct cattcacac 
      tgttgagat actcatgcc tccgagtat acaaatttt 
      tccgaacaa tggggacgt tctgaccac ccggaaggg 
      ggcgacggt gtcgttcga gtgccgcgc cagcgggaa 
      cccaacacc ggtggtcca gtggtcgaa aaaagaagg 
      tctgctacc gtctgggtt acaagtcca aacagggta 
      tctcttatc actaaccaa cgtccaacg cgaagatgc 
      gggcgctta tcagtgcac ggcttccaa cggtatagg 
      gcaggcggt cactggaga aatcaagct ccacgtcct 
      ttttccgcc ggaaattac cgtcgccaa gtcgtgggt 
      gaactccgg ggaaggcct ggaggcccg attggactg 
      cgtcgtcca agcggaccc ccccggaga ggtttcttg 
      gtaccaaaa ttccttccc tttgcaaca aaccgatcg 
      gagaatcat gtcaagtaa aggcaaaat ctacactct 
      gacaataaa aaatgtcca gttctccga tttcgggaa 
      ttacagttg tacggtgca caattcgat cggaaaaga 
      ccggaaata tatcgaatt gaccggaaa gcccggacc 
      ggcacgtat cataagtcc ggcgtattc gaacccgga 
      ttattacga cttgagatg ggtagtgca gtcggtgct 
      gccaatcat tgaagtgaa aattctcta ccgacgaat 
      caatgcaac gacttttta cgaccataa aggccagtg 
      gcacgatct agttataaa gccggagca aaagtacga 
      tgcgaaaac cagtgagag gttgcagag ctttcgcat 
      aacgaattt agtgccaga ttcgttgta cgaatgtct 
      gattttgac caaaaacca gcatgggta cagtgacgt 
      ctcggatgt ccatcagtg gttcagttc ggacaaagg 
      acgaccgtt cgttgggtc aacaggaca aattcattg 
      cttgggaat tgcagtaat gatattgtt gcaaatccc 
      caacgtttt attgtagat agttacgta atgttttta 
      tattgtaca aaatttctt caaataaaa gttacaata 
      attt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY