Status
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Gene information
General information
TC034012
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005898,TC005897
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5876642..5883965
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034012
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09286

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034012 RA
      MSEHIEMSF RGPRVLSAH RRHNFGVYA GPQTNFEGH 
      ANKIVAKLE EDGALMEDT PVSEQLRKE ALRDLPQCL 
      TMKRCVKEK LTKSVSLKS KRRPIGFWK RFKYKSSIR 
      FKKFVANVK NFNYFELWH GSLKDIEGR FGSGYASYF 
      KFLRWLFVM NFFVALFSF PLLVVPQII YDATENNNS 
      NIRQFQASD IFIGNGFFD DTVLYYGHY TNHTIQLIS 
      FLLFDVPVA YFTLIVVLY LISFVILAV SVAQSYRRT 
      FIETKGGLQ NVFANRIFC GWDYSIATK EAAHLKSLA 
      IFNELKELL NEERRYDED RTCIYKFWT CSTQIVANI 
      LILVIIFGV GWLTWVLLD NYGQEDNAK VLITTIVIN 
      VILMVLPSV FHYVATYED YANPRTALY ITLIRTYLL 
      GSVIIGTVL SFWLINSGN TDCWENHLG AEIYRFVLF 
      DFIFSVVLT TVIDSAYFG TLFFLGRDP KTEFNVAKH 
      TLQIIFNQA LFWVGFLYS PILPVIVAL KMFIIWYVR 
      EGCVRKFCK PPARIWRAA QTQTWFMMM VFVGILLVI 
      AAHGYTLKS VPPSSCGPF NGYNYTYEI VTNNIEYLE 
      EEAAVWKVL FNVTKPGGV ALFMVALGI WVYYVKEQA 
      LAQREKVKT MREMLIWMA KDKKFLVQW YNDVTKGEI 
      GTNLRDDRF MDPRKVLPH ECDDDIIVR YVNQNSSKM 
      RNQRNSIPV PTHPVRSSP RALARVPSQ RKSSSSQTE 
      RVVLIKEEN LTFYPCTRL GQNDGGMRR DYVKAVNYI 
      NSCHKFVNA IIEKNNSGN KTATFLLPL TTNYLPLFP 
      YLIGKKPPK KEEIKPLRK TVERQTAKK PPPEPQIPV 
      GDCEEIPQP QQYASHVAD MVKDQIHSF LNHLHEDMN 
      KAVRKTKSE TPKCKNLDI VKQRLEELS KICSLQKKI 
      ESKSQIVAQ GTPPKPPSL PPPLCERLD AGSDISEGE 
      SDEKLSQKM KITCYKESP DISDSSDIK SDSDLEAFL
    CDS sequence
    • >TC034012 RA
      atgtcggaa cacatcgag atgtctttc cggggcccc 
      cgggtgttg tccgcccac cggagacac aacttcggt 
      gtttacgcg ggcccccag accaatttc gaaggccat 
      gcgaataaa atcgtcgcc aaattggag gaagacggg 
      gccttgatg gaggacacc cctgtctct gaacaactg 
      cgaaaggag gccttgagg gacctgccc cagtgcttg 
      accatgaag cggtgcgtc aaagagaaa ttgaccaag 
      tctgtgagt ctcaaatct aaacgaagg ccaataggg 
      ttttggaaa cggttcaaa tacaagtcc agtattaga 
      ttcaaaaaa ttcgttgcc aatgtgaag aatttcaat 
      tatttcgaa ttgtggcac ggcagtttg aaagacatt 
      gaggggcgg tttgggtcc ggttatgcg tcctatttc 
      aagtttttg cgttggctg tttgtaatg aattttttc 
      gtagccttg ttttcgttt ccgttgttg gtagtccct 
      caaattatt tacgacgct actgaaaat aataactca 
      aatattcgg caattccaa gcttcggat atttttatc 
      gggaatggt ttttttgac gacacggta ctttattac 
      ggacactac accaatcat acaatccag ttaatttcg 
      tttttattg tttgacgtg cctgtagcc tatttcaca 
      cttattgta gtgctttat ttgatttct tttgttatt 
      ttggccgta agtgttgcc caatcgtac cgtcgcaca 
      tttatcgag actaaaggt ggtcttcaa aatgttttc 
      gcaaaccga attttctgc ggatgggat tatagtatt 
      gcgaccaaa gaagccgct catttaaaa tccttggca 
      atttttaac gaattaaaa gaattatta aatgaagaa 
      cgtcgatat gatgaggat aggacttgt atttacaaa 
      ttctggact tgtagtaca caaattgtt gcaaatatt 
      ttgattctg gttattatt tttggtgtg ggttggcta 
      acctgggtg ctactcgac aattacgga caagaagac 
      aatgccaaa gtcctaatt acaacaatt gtaatcaat 
      gtaattctt atggtctta ccttcagtc ttccactat 
      gtcgcaaca tacgaggat tatgcaaat ccacgaacg 
      gcactgtat atcacactg atccggacg tatttgcta 
      ggttccgtc attatcggc actgttttg tcattctgg 
      ttgattaat tccggcaac actgactgc tgggaaaac 
      catcttggg gccgaaatt tatcgtttt gttctcttt 
      gatttcatt ttttcagtt gttttgacc acagttatc 
      gattctgca tattttgga acccttttc tttttgggt 
      cgtgaccct aagacagag ttcaacgtt gccaaacat 
      actcttcag attatcttc aatcaggcg ctattctgg 
      gttgggttt ttgtattcg cccattttg ccggtcata 
      gtggcccta aaaatgttc ataatttgg tatgtgagg 
      gaagggtgc gttcgcaaa ttctgtaaa cccccggct 
      cggatttgg cgcgctgcg cagacgcag acgtggttt 
      atgatgatg gtgtttgtt ggtattttg ttggtgatt 
      gccgcccat gggtacacc cttaaaagt gttccaccg 
      tcgagttgt ggtcccttc aatggctat aactatacc 
      tatgaaatt gtgactaac aatattgag tatttggaa 
      gaggaggct gcggtttgg aaagtcctg tttaatgtt 
      actaaaccg ggaggggtg gccttgttt atggttgct 
      ttggggatt tgggtttat tacgtcaag gagcaggcc 
      ctagctcaa agagagaag gttaaaact atgagggaa 
      atgctcata tggatggcc aaagataag aagtttttg 
      gtgcagtgg tataatgat gtaactaaa ggagaaatt 
      ggtacgaat ttgcgtgat gataggttt atggatcct 
      cgcaaagtg ctacctcat gagtgtgat gatgatatt 
      atcgtccgg tatgtgaat caaaattcg tcgaaaatg 
      cgaaatcag cgaaattca attccggtg ccaacacac 
      cccgtgcgt agttccccc agggctttg gcccgagtc 
      ccctctcag cgaaaatcc agcagttcc caaacggag 
      cgcgtcgtc ctcatcaag gaagaaaat ctcactttc 
      tacccttgt acgcgattg gggcaaaac gacggaggg 
      atgcgccga gactacgtc aaagcagtg aattacatc 
      aactcatgc cacaaattt gttaacgcc ataatcgag 
      aaaaacaac tctggtaat aaaaccgca actttcctc 
      ctgccccta accaccaat tacctcccg ctgttccct 
      tacctcatc ggcaaaaag ccgccaaag aaggaggaa 
      atcaagccg ctgcgcaaa accgttgag cgccaaacg 
      gcgaaaaag ccccctccc gagccccaa atccccgtc 
      ggggattgc gaggaaatc ccgcaaccg cagcaatac 
      gcgagtcac gtggccgac atggtcaag gatcaaata 
      cacagtttt ctcaaccat ttgcacgag gatatgaac 
      aaagcggtg cgaaaaacc aagagtgag accccgaag 
      tgcaaaaat ctagatatt gtgaaacaa aggctcgaa 
      gagttgtcg aagatctgc agtttgcag aaaaaaatc 
      gaaagtaaa tcgcaaatt gtggcccag gggactccc 
      cccaagccg ccatctttg ccaccgcca ttgtgcgag 
      cgcttggat gcgggtagc gacatctcg gagggggag 
      agcgacgag aaattgtcg caaaaaatg aaaattacg 
      tgctataag gaaagtccc gatatttcg gactcttcg 
      gatatcaag tccgattcg gacctggaa gcgtttttg 
      taa
    mRNA sequence
    • >TC034012 RA
      acctcaaaa aataatgta ttaatttat ttttgttct 
      taattaaca aaaacagtt acaaatgtg atgactcag 
      acggtacgc ttcgcgttt tgttgttta cttttttcc 
      ttatttgtg ctcttaatt cggcttaat aaccttatc 
      acggaagtg cccgcaaat aaacttatc acttaatcg 
      cttccaaat tactgatag cgcacacaa tgcacttta 
      tcaatccca gtttgtgcc gcgtttcgt agcccccat 
      gtcggaaca catcgagat gtctttccg gggcccccg 
      ggtgttgtc cgcccaccg gagacacaa cttcggtgt 
      ttacgcggg cccccagac caatttcga aggccatgc 
      gaataaaat cgtcgccaa attggagga agacggggc 
      cttgatgga ggacacccc tgtctctga acaactgcg 
      aaaggaggc cttgaggga cctgcccca gtgcttgac 
      catgaagcg gtgcgtcaa agagaaatt gaccaagtc 
      tgtgagtct caaatctaa acgaaggcc aatagggtt 
      ttggaaacg gttcaaata caagtccag tattagatt 
      caaaaaatt cgttgccaa tgtgaagaa tttcaatta 
      tttcgaatt gtggcacgg cagtttgaa agacattga 
      ggggcggtt tgggtccgg ttatgcgtc ctatttcaa 
      gtttttgcg ttggctgtt tgtaatgaa ttttttcgt 
      agccttgtt ttcgtttcc gttgttggt agtccctca 
      aattattta cgacgctac tgaaaataa taactcaaa 
      tattcggca attccaagc ttcggatat ttttatcgg 
      gaatggttt ttttgacga cacggtact ttattacgg 
      acactacac caatcatac aatccagtt aatttcgtt 
      tttattgtt tgacgtgcc tgtagccta tttcacact 
      tattgtagt gctttattt gatttcttt tgttatttt 
      ggccgtaag tgttgccca atcgtaccg tcgcacatt 
      tatcgagac taaaggtgg tcttcaaaa tgttttcgc 
      aaaccgaat tttctgcgg atgggatta tagtattgc 
      gaccaaaga agccgctca tttaaaatc cttggcaat 
      ttttaacga attaaaaga attattaaa tgaagaacg 
      tcgatatga tgaggatag gacttgtat ttacaaatt 
      ctggacttg tagtacaca aattgttgc aaatatttt 
      gattctggt tattatttt tggtgtggg ttggctaac 
      ctgggtgct actcgacaa ttacggaca agaagacaa 
      tgccaaagt cctaattac aacaattgt aatcaatgt 
      aattcttat ggtcttacc ttcagtctt ccactatgt 
      cgcaacata cgaggatta tgcaaatcc acgaacggc 
      actgtatat cacactgat ccggacgta tttgctagg 
      ttccgtcat tatcggcac tgttttgtc attctggtt 
      gattaattc cggcaacac tgactgctg ggaaaacca 
      tcttggggc cgaaattta tcgttttgt tctctttga 
      tttcatttt ttcagttgt tttgaccac agttatcga 
      ttctgcata ttttggaac ccttttctt tttgggtcg 
      tgaccctaa gacagagtt caacgttgc caaacatac 
      tcttcagat tatcttcaa tcaggcgct attctgggt 
      tgggttttt gtattcgcc cattttgcc ggtcatagt 
      ggccctaaa aatgttcat aatttggta tgtgaggga 
      agggtgcgt tcgcaaatt ctgtaaacc cccggctcg 
      gatttggcg cgctgcgca gacgcagac gtggtttat 
      gatgatggt gtttgttgg tattttgtt ggtgattgc 
      cgcccatgg gtacaccct taaaagtgt tccaccgtc 
      gagttgtgg tcccttcaa tggctataa ctataccta 
      tgaaattgt gactaacaa tattgagta tttggaaga 
      ggaggctgc ggtttggaa agtcctgtt taatgttac 
      taaaccggg aggggtggc cttgtttat ggttgcttt 
      ggggatttg ggtttatta cgtcaagga gcaggccct 
      agctcaaag agagaaggt taaaactat gagggaaat 
      gctcatatg gatggccaa agataagaa gtttttggt 
      gcagtggta taatgatgt aactaaagg agaaattgg 
      tacgaattt gcgtgatga taggtttat ggatcctcg 
      caaagtgct acctcatga gtgtgatga tgatattat 
      cgtccggta tgtgaatca aaattcgtc gaaaatgcg 
      aaatcagcg aaattcaat tccggtgcc aacacaccc 
      cgtgcgtag ttcccccag ggctttggc ccgagtccc 
      ctctcagcg aaaatccag cagttccca aacggagcg 
      cgtcgtcct catcaagga agaaaatct cactttcta 
      cccttgtac gcgattggg gcaaaacga cggagggat 
      gcgccgaga ctacgtcaa agcagtgaa ttacatcaa 
      ctcatgcca caaatttgt taacgccat aatcgagaa 
      aaacaactc tggtaataa aaccgcaac tttcctcct 
      gcccctaac caccaatta cctcccgct gttccctta 
      cctcatcgg caaaaagcc gccaaagaa ggaggaaat 
      caagccgct gcgcaaaac cgttgagcg ccaaacggc 
      gaaaaagcc ccctcccga gccccaaat ccccgtcgg 
      ggattgcga ggaaatccc gcaaccgca gcaatacgc 
      gagtcacgt ggccgacat ggtcaagga tcaaataca 
      cagttttct caaccattt gcacgagga tatgaacaa 
      agcggtgcg aaaaaccaa gagtgagac cccgaagtg 
      caaaaatct agatattgt gaaacaaag gctcgaaga 
      gttgtcgaa gatctgcag tttgcagaa aaaaatcga 
      aagtaaatc gcaaattgt ggcccaggg gactccccc 
      caagccgcc atctttgcc accgccatt gtgcgagcg 
      cttggatgc gggtagcga catctcgga gggggagag 
      cgacgagaa attgtcgca aaaaatgaa aattacgtg 
      ctataagga aagtcccga tatttcgga ctcttcgga 
      tatcaagtc cgattcgga cctggaagc gtttttgta 
      aatttcgta aagtttgta cacacaatg aattctcta 
      ataaattta aaattacgc a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY