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Gene information
General information
TC030639 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5172287..5176296
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030639
    iBeetle screen iB_09281

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030639 RA
      MISNTVIEQ CCFYFNKLT SLDTWKRIQ 
      WMNKKAPPQ EAYRPTPIE HIANVVTHG 
      VWVIPSVLA TLELLERSR NGAQTMSAL 
      VYGATLVFL FSVSTSFHC SFFCNQRGK 
      LKDVLHRCD RAMIYVFIA GSYFPWLTL 
      EQLPQDGWA SFMNWFVWL LALLGIIYQ 
      QTFHEKYKT LETCFYLIM GIGPRLITN 
      FRARIFRTD GAKNRRCSL RNGGDVFQK
    CDS sequence
    • >TC030639 RA
      atgataagt aacactgtc atcgagcag tgttgcttc 
      tatttcaac aaactcacc tctttggac acatggaaa 
      aggatacaa tggatgaac aaaaaggcc ccaccgcag 
      gaagcttac agaccgaca cccatcgaa cacattgcc 
      aacgttgtc acccacggc gtatgggtc atacctagc 
      gtcctcgcc acccttgaa ctacttgaa cgatctcga 
      aatggtgct caaaccatg tcggcgctg gtttatggg 
      gccacactt gtgtttctc ttcagcgtt tcgacgtcg 
      tttcactgc tcctttttc tgcaaccag cgcggaaag 
      ctcaaagat gtcctccac cgatgtgat agggctatg 
      atttacgtg ttcatagcc ggctcatat ttcccctgg 
      ctgaccctc gagcagctc cctcaggac ggctgggct 
      tccttcatg aattggttt gtctggctg ttggccctt 
      ttaggcata atttatcaa cagacgttc cacgagaaa 
      tacaaaact ttggaaact tgcttctat ctaattatg 
      gggattggc ccccggctt attactaat ttcagagca 
      cgaattttc cggactgac ggagctaaa aatcggcgg 
      tgctctcta cgtaatggg ggtgatgtt tttcaaaag 
      tga
    mRNA sequence
    • >TC030639 RA
      cctgtcact tgtcaaaaa caataacaa acccagttc 
      aaagggcaa agggtggcc gagggggcc tgctttgtt 
      tatccatta cctggaatg ataagtaac actgtcatc 
      gagcagtgt tgcttctat ttcaacaaa ctcacctct 
      ttggacaca tggaaaagg atacaatgg atgaacaaa 
      aaggcccca ccgcaggaa gcttacaga ccgacaccc 
      atcgaacac attgccaac gttgtcacc cacggcgta 
      tgggtcata cctagcgtc ctcgccacc cttgaacta 
      cttgaacga tctcgaaat ggtgctcaa accatgtcg 
      gcgctggtt tatggggcc acacttgtg tttctcttc 
      agcgtttcg acgtcgttt cactgctcc tttttctgc 
      aaccagcgc ggaaagctc aaagatgtc ctccaccga 
      tgtgatagg gctatgatt tacgtgttc atagccggc 
      tcatatttc ccctggctg accctcgag cagctccct 
      caggacggc tgggcttcc ttcatgaat tggtttgtc 
      tggctgttg gccctttta ggcataatt tatcaacag 
      acgttccac gagaaatac aaaactttg gaaacttgc 
      ttctatcta attatgggg attggcccc cggcttatt 
      actaatttc agagcacga attttccgg actgacgga 
      gctaaaaat cggcggtgc tctctacgt aatgggggt 
      gatgttttt caaaagtga tggaattat cccttgtgc 
      tcacgcaat ttggcattt attcgtcgt aatggccgc 
      aggggtgca ctattacgc cattcggaa taatttgta 
      tccagttaa caccttttt gttgaaaaa ttaagtaat 
      actcgaaag cgtgacgtc ttccgctga attatttta 
      gcgtcgctt ttgttgtgc aataaaaac tgctcaaat 
      ttggtcttc agaatactc atcagtgtc ggatcaatt 
      taagttggc aacgccgaa taacaagag agtttgttg 
      ccaacttaa tttcaaact gtggcgccg aaatgtacc 
      taaaatggt aacagagga gtcaagttt taatactat 
      tttaggttc gtttcttca accgatctt gtgattgta 
      tacaataaa tatctcgtt tatcagtga tgtactctt 
      ccaaagaag ctccactat aattattat tgtaaataa 
      aaaaatcgt atatgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY