Status
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Gene information
General information
TC033995
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC005948,TC005947
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 5061133..5073828
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033995
    iBeetle screen iB_09278

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    antenna shape swollen - Penetrance (percentage of animals/eggs on slide): more than 80%

    mandible shape swollen - Penetrance (percentage of animals/eggs on slide): more than 80%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    head appendages partially not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    Comment: Head appendages are mostly not present except antenna & mandible. Antenna & mandible segments are partially not present.
    head shape swollen - Penetrance (percentage of animals/eggs on slide): more than 80%

    head size shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs number increased - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs shape swollen - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    thorax shape swollen - Penetrance (percentage of animals/eggs on slide): more than 80%
    thorax size shortened - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen segment transformed - Penetrance (percentage of animals/eggs on slide): more than 80%
    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    Comment: Head segments are also partially absent. It's not possible to use head, abdomen & terminus combination in the annotation.
    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    Comment: Head segments are also partially absent. It's not possible to use head, abdomen & terminus combination in the annotation.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033995 RA
      MASSSTFEA KVLMAKGKR ATAAYIHAD CSSSPCPQH 
      LNAVLDSLL NPARSIDEW ETIDWCKWL MAGGRTPDE 
      FANTVRAYD NQTTCGLVW TPNFVAYRC RTCGISPCM 
      SLCTDCFKK GNHQRHDFN MFLSQAGGA CDCGDTSVM 
      KETGFCDQH GPDKSANKG KAPNDLMCV AEAMMPRII 
      FRFLLHLRE NCHMKGPIQ DADGFITML VDFNNMGAL 
      MRRVMTSAL TNPQKYRDL NEIPQNCDS EYAAYLTES 
      KRIYEEALA SLANPEPLE EYKDCPSLQ RNLAHKTFL 
      EELVFWTVM FEFPEKVVC LLLNMLPDP DYKEALMRA 
      IVLHYSRIS MMLERTNDP ENVSNRVVH ASVQLFSNE 
      SLALRMTEQ LNLLHVMVV SLRYMMSKI LVENSLHAP 
      ENNFHYVVD CSKRVMTDH CYWPLVSDL NNVLSHRPV 
      ALKFMSDDM LIKMWFGFL GMFQGMNVN QRETKEHVK 
      FEPNTYFAA FSAELEASA YPMWALVSH LTDASTADL 
      TRKVLSACL RELRSWLEA INFTCLNMN DNLQVSFHL 
      PLHRYFSVF LCQAVAKQG ITLDEVLPS NEDLMVMIM 
      HPLRVQVSV YIIFYESIP KFFFKLCYF G
    CDS sequence
    • >TC033995 RA
      atggcctca agttctact ttcgaggcg aaagtgctg 
      atggccaaa ggcaagcga gcaacggcc gcttacatc 
      cacgccgac tgctccagc tcgccgtgc ccccagcac 
      ctcaacgcc gtcctcgac agcctcctc aaccccgcg 
      cggtccatc gacgagtgg gagaccatc gactggtgc 
      aaatggctg atggccggg ggtcggacc cccgacgaa 
      ttcgccaac acagttcga gcgtacgac aaccaaacg 
      acctgtggc ctcgtctgg acgcccaac tttgtggcc 
      tatcgatgt cgcacctgc ggcatatca ccctgtatg 
      tcgctatgt accgactgt ttcaaaaaa ggcaaccat 
      cagcgacac gacttcaac atgtttctg agtcaagcg 
      ggcggtgcg tgcgattgt ggcgacacc tcggtcatg 
      aaggagaca gggttttgc gatcaacac ggccctgac 
      aaatcggca aataagggt aaagcaccg aacgatctg 
      atgtgtgtc gctgaggcg atgatgccc cggattatt 
      tttcggttt cttttacat ttgagggag aactgtcat 
      atgaaaggt ccaatccag gacgcggat ggttttatt 
      accatgttg gtggatttt aacaacatg ggggcgctg 
      atgcgacgc gtgatgaca tcagcattg accaatccg 
      cagaaatat cgcgacttg aacgaaatc ccccaaaat 
      tgcgattcg gaatatgcc gcctatctc accgaatcg 
      aaacgaatc tacgaagaa gcgttggct tcgttagcc 
      aatccggaa ccgttagaa gagtataaa gactgtcct 
      tcgctccag aggaatttg gcgcataag acgttcctc 
      gaagaactt gttttctgg actgttatg tttgaattt 
      cccgaaaaa gtcgtctgt ttgctcttg aatatgtta 
      ccggacccg gattacaag gaggctttg atgcgcgca 
      attgtttta cactatagt cgcatttcg atgatgttg 
      gaacggacg aatgatccc gaaaacgtc agtaatcga 
      gtcgttcac gctagcgtg caattattc tcgaatgaa 
      agtctcgcg ttgcgtatg accgaacaa ttgaatctc 
      ttacacgtg atggtggtg agtttgagg tacatgatg 
      agtaagatt ttggtcgag aatagtctc cacgccccc 
      gaaaataat ttccattac gtggtcgat tgcagcaag 
      cgtgtcatg acggatcat tgctattgg ccattagtt 
      agcgattta aacaatgtg ctgtcgcac cgtcctgtc 
      gcgcttaaa ttcatgtcc gatgatatg ctgatcaag 
      atgtggttt ggtttcttg ggcatgttt caaggcatg 
      aatgtcaat caacgtgaa actaaagaa cacgttaaa 
      tttgaacct aatacttat ttcgcggcg tttagtgcc 
      gaactggaa gctagtgct tatccaatg tgggcgcta 
      gtgtcgcat ttgacggat gcgagtacg gccgatttg 
      acacgaaaa gtgctttcg gcctgtttg agggaattg 
      aggagttgg ctcgaagcg atcaatttt acttgttta 
      aatatgaac gataatcta caagtgtcg tttcatttg 
      ccacttcat cgctacttt tcggttttt ttgtgtcaa 
      gctgttgcc aaacagggg attaccttg gatgaggtt 
      ttgccatcg aatgaggac ctgatggtt atgattatg 
      catccactg agggttcag gtgagtgta tacattatt 
      ttttacgaa tcaataccg aaatttttc tttaaactt 
      tgttatttt ggctga
    mRNA sequence
    • >TC033995 RA
      acatttcac acaataatg taataatta ttattaatg 
      taaatggct cacaagctc cccgttttt gttggtgtt 
      ggtaccgct gtttttagt aatttttat tgtcaacag 
      gctggaatt ccgtttaaa atcggcgga aatttcgtt 
      gttgagatg tttgtgatt gcgctgggt gtaaaaatt 
      tggttttgc gtgcgtaat aattgttcc tcttggagg 
      attttgagt taaggacgg cgtgctggt ggttttggt 
      gtcgttatg ttggtgtca agttcagga aggtgtcat 
      ggtggtcaa gcgttaacc tcgcgcaac aaaatattc 
      ttattcgcg ttgtgaata gagctgttt ttggcgtgt 
      tttcaaagt tctatgact gttggaaat atttagcat 
      ttgtgtgac gacatactc gtgcaagtg agacaacct 
      gagcgaggt cgcctcgag aatcactcc gtttcattt 
      tacgcgagt ttccaagtt gtccggttg ctaaatccg 
      tgtgaaacc gtgttagaa gtaaatctg ttgatggag 
      ttgactcgc ggtcgtgcg ctaatgtcg cgagtgagc 
      tgtgaaaaa gtgaagttg atgcggcag tgaaattgc 
      tgatgtgat gacagttta gtggcgaaa atggcctca 
      agttctact ttcgaggcg aaagtgctg atggccaaa 
      ggcaagcga gcaacggcc gcttacatc cacgccgac 
      tgctccagc tcgccgtgc ccccagcac ctcaacgcc 
      gtcctcgac agcctcctc aaccccgcg cggtccatc 
      gacgagtgg gagaccatc gactggtgc aaatggctg 
      atggccggg ggtcggacc cccgacgaa ttcgccaac 
      acagttcga gcgtacgac aaccaaacg acctgtggc 
      ctcgtctgg acgcccaac tttgtggcc tatcgatgt 
      cgcacctgc ggcatatca ccctgtatg tcgctatgt 
      accgactgt ttcaaaaaa ggcaaccat cagcgacac 
      gacttcaac atgtttctg agtcaagcg ggcggtgcg 
      tgcgattgt ggcgacacc tcggtcatg aaggagaca 
      gggttttgc gatcaacac ggccctgac aaatcggca 
      aataagggt aaagcaccg aacgatctg atgtgtgtc 
      gctgaggcg atgatgccc cggattatt tttcggttt 
      cttttacat ttgagggag aactgtcat atgaaaggt 
      ccaatccag gacgcggat ggttttatt accatgttg 
      gtggatttt aacaacatg ggggcgctg atgcgacgc 
      gtgatgaca tcagcattg accaatccg cagaaatat 
      cgcgacttg aacgaaatc ccccaaaat tgcgattcg 
      gaatatgcc gcctatctc accgaatcg aaacgaatc 
      tacgaagaa gcgttggct tcgttagcc aatccggaa 
      ccgttagaa gagtataaa gactgtcct tcgctccag 
      aggaatttg gcgcataag acgttcctc gaagaactt 
      gttttctgg actgttatg tttgaattt cccgaaaaa 
      gtcgtctgt ttgctcttg aatatgtta ccggacccg 
      gattacaag gaggctttg atgcgcgca attgtttta 
      cactatagt cgcatttcg atgatgttg gaacggacg 
      aatgatccc gaaaacgtc agtaatcga gtcgttcac 
      gctagcgtg caattattc tcgaatgaa agtctcgcg 
      ttgcgtatg accgaacaa ttgaatctc ttacacgtg 
      atggtggtg agtttgagg tacatgatg agtaagatt 
      ttggtcgag aatagtctc cacgccccc gaaaataat 
      ttccattac gtggtcgat tgcagcaag cgtgtcatg 
      acggatcat tgctattgg ccattagtt agcgattta 
      aacaatgtg ctgtcgcac cgtcctgtc gcgcttaaa 
      ttcatgtcc gatgatatg ctgatcaag atgtggttt 
      ggtttcttg ggcatgttt caaggcatg aatgtcaat 
      caacgtgaa actaaagaa cacgttaaa tttgaacct 
      aatacttat ttcgcggcg tttagtgcc gaactggaa 
      gctagtgct tatccaatg tgggcgcta gtgtcgcat 
      ttgacggat gcgagtacg gccgatttg acacgaaaa 
      gtgctttcg gcctgtttg agggaattg aggagttgg 
      ctcgaagcg atcaatttt acttgttta aatatgaac 
      gataatcta caagtgtcg tttcatttg ccacttcat 
      cgctacttt tcggttttt ttgtgtcaa gctgttgcc 
      aaacagggg attaccttg gatgaggtt ttgccatcg 
      aatgaggac ctgatggtt atgattatg catccactg 
      agggttcag gtgagtgta tacattatt ttttacgaa 
      tcaataccg aaatttttc tttaaactt tgttatttt 
      ggctgagcg tctgagtta aattaacta tggcttttt 
      tacgagctt aagatgaac cgagccaaa aaatgggtg 
      aaaaataat aaaatttat acctgctg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY