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Gene information
General information
TC006133 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2920856..2922577
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006133
    iBeetle screen iB_09275

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006133 RA
      MANSETPLQ ASRKNAYYR SIFDKYDRD HDGFINIGE 
      LNDLIESRE YDHDIPEHV VRKIHQMAD QNHDDRIDF 
      TEFVDMINH PDLQYVFGH YVNRYIQMV VPRRRGASE 
      VDGAYDDQY SCYPPAVGM IIISLIEVI FFCVDEAIE 
      ADSTKSASG PISSVFIYD PYKRQEAWR FITYMFVHV 
      GAFHLFVNL LVQILLGTP LEMVHRWWR VLLIYFAGV 
      LAGSLATSI ADPTVRLAG ASGGVYSLI TAHIATIIM 
      NWREMSFPI VQLFIFLII MASDIGTAI YNRYFLEMD 
      EHIGYMAHF AGAIAGLLV GLNVLRNLE VTTVERVIW 
      WLSIVTYCL LMAIAIIWN FAWPEYFLK SRERI
    CDS sequence
    • >TC006133 RA
      atggccaac tcggagacg ccgctccaa gccagccgc 
      aaaaacgcc tactaccgc agcattttc gacaaatac 
      gaccgggac catgacggt tttatcaac atcggggag 
      ctgaatgac ctgatcgag agccgggaa tatgaccac 
      gacatcccc gagcacgtc gtgcggaaa atccaccaa 
      atggccgat cagaaccac gacgaccga atcgatttc 
      accgaattc gtggacatg ataaaccac ccggacctc 
      cagtacgtt ttcgggcac tatgtcaac aggtacatc 
      cagatggtg gtgccccgc cgacggggc gccagcgag 
      gtggacggg gcctacgac gaccagtac tcctgctac 
      cccccggcc gtgggcatg atcattatt tcgctaatc 
      gaggtcatt tttttctgt gtggatgag gcaatcgag 
      gcggattcg accaaatcg gcctcgggc cccatcagt 
      tccgtcttc atctacgac ccctacaag agacaggag 
      gcttggcgg ttcatcact tacatgttt gtgcacgtt 
      ggagccttc catttgttt gtcaatctt ttggtgcaa 
      attctactg gggactccg ctagaaatg gtgcaccgg 
      tggtggcga gtgctcctc atttatttc gcgggggtc 
      ctcgccggg tcgcttgct acctcaatc gccgacccc 
      accgtgaga cttgcgggg gccagcggt ggggtctac 
      tccctcatc acggcacat atagccacc attataatg 
      aattggagg gaaatgtcg ttccccatc gtccagtta 
      tttattttt ttgatcatt atggcctcc gacattggg 
      accgccatt tacaatcgg tatttcctc gaaatggac 
      gaacatatc gggtacatg gcccacttt gcgggggct 
      attgccggg ctcttggtg gggctcaac gtcctaaga 
      aacctagaa gtgacaacg gtcgaacgg gtcatttgg 
      tggctctct atcgtcacg tattgtctc ctgatggca 
      atcgcaatc atttggaat tttgcctgg cctgagtat 
      tttttgaaa agtcgggaa cggatttga
    mRNA sequence
    • >TC006133 RA
      agttaccaa aacataacc gtgtaatga taattaaca 
      aaacaaaag tgataataa taataagtg ataacatgt 
      agtaacacg agtgttagt aacgtaaac agtgattgt 
      gtgtgtgcg ccaattgag gttatgtgg ccatggcca 
      actcggaga cgccgctcc aagccagcc gcaaaaacg 
      cctactacc gcagcattt tcgacaaat acgaccggg 
      accatgacg gttttatca acatcgggg agctgaatg 
      acctgatcg agagccggg aatatgacc acgacatcc 
      ccgagcacg tcgtgcgga aaatccacc aaatggccg 
      atcagaacc acgacgacc gaatcgatt tcaccgaat 
      tcgtggaca tgataaacc acccggacc tccagtacg 
      ttttcgggc actatgtca acaggtaca tccagatgg 
      tggtgcccc gccgacggg gcgccagcg aggtggacg 
      gggcctacg acgaccagt actcctgct accccccgg 
      ccgtgggca tgatcatta tttcgctaa tcgaggtca 
      tttttttct gtgtggatg aggcaatcg aggcggatt 
      cgaccaaat cggcctcgg gccccatca gttccgtct 
      tcatctacg acccctaca agagacagg aggcttggc 
      ggttcatca cttacatgt ttgtgcacg ttggagcct 
      tccatttgt ttgtcaatc ttttggtgc aaattctac 
      tggggactc cgctagaaa tggtgcacc ggtggtggc 
      gagtgctcc tcatttatt tcgcggggg tcctcgccg 
      ggtcgcttg ctacctcaa tcgccgacc ccaccgtga 
      gacttgcgg gggccagcg gtggggtct actccctca 
      tcacggcac atatagcca ccattataa tgaattgga 
      gggaaatgt cgttcccca tcgtccagt tatttattt 
      ttttgatca ttatggcct ccgacattg ggaccgcca 
      tttacaatc ggtatttcc tcgaaatgg acgaacata 
      tcgggtaca tggcccact ttgcggggg ctattgccg 
      ggctcttgg tggggctca acgtcctaa gaaacctag 
      aagtgacaa cggtcgaac gggtcattt ggtggctct 
      ctatcgtca cgtattgtc tcctgatgg caatcgcaa 
      tcatttgga attttgcct ggcctgagt attttttga 
      aaagtcggg aacggattt gaggttagg aaatgacac 
      aaaagtgta acaattatt atttttaag cacagatta 
      ggtacaaag tgaccagtt tttgcagta ttgtatgac 
      tagaggttg attttagta ttatttttg attttgtaa 
      aagtggggt tatgtcggc gcataatca gaaagtgtt 
      ttatttgag cattgcgcg agcccccaa ctcatctgg 
      acggactcg caaccgtgt ttcaagccc ctagtatta 
      aggtcagcc ccctcaaac atttttttg taagcaatt 
      taatatgca aagtaaaag tgaggttat gtttgacaa 
      tccacgaaa aggcctagt tatagtatt tgcggtgcc 
      ttcctagca caaaactct agtattttt tatattcta 
      ctttattac gtccaaata tttacaaca ataaaagac 
      ttattactt g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY