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Gene information
General information
TC034818 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2592919..2596366
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC034818
    iBeetle screen iB_09273

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    previtellogenic egg chamber anterior partiallyshape constricted - Number of animals showing the phenotype: 4

    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 4

    mature egg potentially not present - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034818 RA
      MASRKKVLL KVIILGDSS VGKTSLMNQ 
      YVNRKFSNQ YKATIGADF LTKEVTVDD 
      RSVTMQIWD TAGQERFQS LGVAFYRGA 
      DCCVLVFDV TAPNTFKSL DSWRDEFLI 
      QASPREPEN FPFVLLGNK VDLEPRAIS 
      SKRAQQWCQ MKNNIPYFE TSAKEGLNV 
      EQAFLAIAK NALAQQNST VDLYNEFPD 
      QIKLTNTNK PNSNGDSCA C
    CDS sequence
    • >TC034818 RA
      atggcgagt cgtaaaaaa gtcttgttg aaagtcatc 
      attttgggc gactcaagt gtcggaaaa acctctctc 
      atgaatcag tatgttaat cgaaagttt tccaatcaa 
      tacaaagca acgatcggt gctgatttc cttacgaaa 
      gaagtaaca gtcgatgac aggtccgta acgatgcaa 
      atctgggac accgccgga caggagcgg ttccaatcg 
      ctcggggtg gccttttac aggggcgca gactgttgc 
      gtcttggtg tttgacgtt acggcgcct aacacgttt 
      aaatcattg gacagttgg cgcgacgag tttttgatc 
      caggcgtcg ccccgcgag cccgaaaac ttccccttt 
      gtgcttttg ggcaataaa gtggacttg gagccgcgg 
      gctatatca tcaaagagg gcgcagcaa tggtgccag 
      atgaagaac aacatcccc tatttcgaa acgtccgcg 
      aaagaaggg ctcaacgtg gagcaggcg tttttggca 
      attgcgaaa aacgctcta gctcaacaa aactcgacc 
      gttgatctt tataatgaa tttcccgat caaatcaaa 
      cttactaat acaaataag ccgaatagt aacggagat 
      tcttgcgca tgttag
    mRNA sequence
    • >TC034818 RA
      acgacgcca ttttcgtta gttgtacgc aataaatca 
      ctcagtgta tttttcccc aaatttcaa gaaaattca 
      aactcaaaa aagatggcg agtcgtaaa aaagtcttg 
      ttgaaagtc atcattttg ggcgactca agtgtcgga 
      aaaacctct ctcatgaat cagtatgtt aatcgaaag 
      ttttccaat caatacaaa gcaacgatc ggtgctgat 
      ttccttacg aaagaagta acagtcgat gacaggtcc 
      gtaacgatg caaatctgg gacaccgcc ggacaggag 
      cggttccaa tcgctcggg gtggccttt tacaggggc 
      gcagactgt tgcgtcttg gtgtttgac gttacggcg 
      cctaacacg tttaaatca ttggacagt tggcgcgac 
      gagtttttg atccaggcg tcgccccgc gagcccgaa 
      aacttcccc tttgtgctt ttgggcaat aaagtggac 
      ttggagccg cgggctata tcatcaaag agggcgcag 
      caatggtgc cagatgaag aacaacatc ccctatttc 
      gaaacgtcc gcgaaagaa gggctcaac gtggagcag 
      gcgtttttg gcaattgcg aaaaacgct ctagctcaa 
      caaaactcg accgttgat ctttataat gaatttccc 
      gatcaaatc aaacttact aatacaaat aagccgaat 
      agtaacgga gattcttgc gcatgttag gttgggttt 
      tctgctcaa tgtgatata acttttttt tctcctatt 
      attactttg gttttcgca aactcaccg aaattgttg 
      gtttacatg aattgtgta aatacaatt gttgaacga 
      aaggaattt ttattatat actttttgc ttttgtaga 
      gaatttcgt atcggcagt aaaggtttt cctcaagtt 
      aagttttgt tattattaa atatcgagt gttctgtgt 
      tgtaggcta attatttat agtaaatag aaaatttat 
      atatttttt ttaaccatt aaatacaac ttttctgta 
      gaatatatc ataaaattt gta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY