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Gene information
General information
TC006097 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007423.3: 2423638..2424802
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC006097
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_09272

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Adult morphology
    odoriferous glands colour darker - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    abdominal odoriferous glands anterior content darker - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    abdominal odoriferous glands necrotic - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection


    abdominal odoriferous glands content not present - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC006097 RA
      MSLTQRQIA KILKQAQSG DLAPISALK THWSVLKYQ 
      DTGDTILHC ATRLGHLET VQYLLSFEP PGVDCTNND 
      NKTALHEAA QFSQPEIMK LLLDKGAQV NALKRADWT 
      PLMLACTKT CLETVRVLV ESGALINYR NKDGWTCMH 
      LAARGGCGA MFTFLVTKG GDCALRTKN GRTVLHIAA 
      LHGSFEIVK ILLKLGVLP IDVRDNCGN TALHEAVLG 
      RHKNICSLL IQNGADIKC TNNVDFNLL FLASSEGYV 
      DLAEYIILE LGFDINITN SNGFTALHC AARKQQKDM 
      CQYLVKMGA NRGAKDHFR RVAFDYLTT
    CDS sequence
    • >TC006097 RA
      atgtcgcta acccagcgc caaatcgcc aaaatcctc 
      aaacaggca cagtcgggg gacctcgcc cccatctcg 
      gcgttgaaa acgcactgg agcgtgtta aaataccaa 
      gacaccggt gacacaatc ctgcattgc gccacccgt 
      ttggggcac cttgaaacc gtccaatac ctcctgtcg 
      ttcgagccc ccaggcgtc gactgtacc aacaacgac 
      aacaaaacg gccctacac gaagccgcc cagttctca 
      caacccgaa atcatgaaa ctgctcctc gacaagggg 
      gctcaagtc aacgccttg aaacgcgcc gattggacc 
      cctttgatg ctggcttgc accaaaacg tgtctcgag 
      actgtgcga gttttggtg gaaagtggc gctttgatt 
      aattacaga aacaaagac gggtggact tgtatgcat 
      ttggcggca cgagggggc tgtggggcc atgtttacg 
      tttctggtc accaaaggg ggcgattgt gcattgagg 
      actaaaaac ggcaggaca gtgctacac atcgctgcg 
      ctacatggc agttttgaa attgttaaa attttgctc 
      aagttgggg gttttaccc attgatgtt agggacaat 
      tgtggtaac accgcattg cacgaagcg gtgctaggg 
      cggcacaaa aatatttgt agtttgttg atacaaaac 
      ggcgctgat attaaatgt accaataat gttgatttc 
      aatttactg tttttggct agtagtgag ggttacgtt 
      gatttagcc gagtatatt attcttgag ttaggtttt 
      gacataaat attactaat tcaaatggt tttactgcg 
      ttacattgt gcggctaga aagcagcaa aaggatatg 
      tgtcaatat ttagtcaaa atgggggct aatcgaggg 
      gcaaaggat catttcaga agggttgcc tttgactat 
      cttactacc tga
    mRNA sequence
    • >TC006097 RA
      ccgctttag ataaactaa ttaattaac aattgttca 
      ctttattta aaaacacga ccacactta tctattgca 
      agatttaat ttatgtatt tagtaaacc taacctcac 
      gccttgaca gttgtttaa cctaaaaaa tgtcgctaa 
      cccagcgcc aaatcgcca aaatcctca aacaggcac 
      agtcggggg acctcgccc ccatctcgg cgttgaaaa 
      cgcactgga gcgtgttaa aataccaag acaccggtg 
      acacaatcc tgcattgcg ccacccgtt tggggcacc 
      ttgaaaccg tccaatacc tcctgtcgt tcgagcccc 
      caggcgtcg actgtacca acaacgaca acaaaacgg 
      ccctacacg aagccgccc agttctcac aacccgaaa 
      tcatgaaac tgctcctcg acaaggggg ctcaagtca 
      acgccttga aacgcgccg attggaccc ctttgatgc 
      tggcttgca ccaaaacgt gtctcgaga ctgtgcgag 
      ttttggtgg aaagtggcg ctttgatta attacagaa 
      acaaagacg ggtggactt gtatgcatt tggcggcac 
      gagggggct gtggggcca tgtttacgt ttctggtca 
      ccaaagggg gcgattgtg cattgagga ctaaaaacg 
      gcaggacag tgctacaca tcgctgcgc tacatggca 
      gttttgaaa ttgttaaaa ttttgctca agttggggg 
      ttttaccca ttgatgtta gggacaatt gtggtaaca 
      ccgcattgc acgaagcgg tgctagggc ggcacaaaa 
      atatttgta gtttgttga tacaaaacg gcgctgata 
      ttaaatgta ccaataatg ttgatttca atttactgt 
      ttttggcta gtagtgagg gttacgttg atttagccg 
      agtatatta ttcttgagt taggttttg acataaata 
      ttactaatt caaatggtt ttactgcgt tacattgtg 
      cggctagaa agcagcaaa aggatatgt gtcaatatt 
      tagtcaaaa tgggggcta atcgagggg caaaggatc 
      atttcagaa gggttgcct ttgactatc ttactacct 
      gaggctgct ttatttggc gccatctgg taacgtcgc 
      tgttaccat ggtaactga ggcggatta caggctcct 
      gcattatca aaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY